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The map label for this gene is metK1

Identifier: 162447194

GI number: 162447194

Start: 338497

End: 339657

Strand: Direct

Name: metK1

Synonym: ACL_0328

Alternate gene names: 162447194

Gene position: 338497-339657 (Clockwise)

Preceding gene: 162447193

Following gene: 162447195

Centisome position: 22.61

GC content: 33.68

Gene sequence:

>1161_bases
ATGAGTTATATTTTTTCAAGCGAAAGTGTGTCTAAAGGACATCCAGATAAAGTGGCGGATTTTATAGCAGATTCAGTATT
AGATTTAGTATTAAAAAATGACCCTAAAGCACGTTGTGCGATTGAGGTTGCTTTAGCACACACAACTGTTTATATTTTTG
GTGAGGTATCAACAAGTTATATATTAACAGATGAAATCATTATTGAAACTGCAAAAAAAGCAATCGATTTTTGTGGTTAT
AATGATGAGAAATTCATCTTTGATGCAAAATCAGCAACCTATCATGTAAACATTCATAAACAAAGTGCAGATATCGCGCT
AGGGGTTGATGAAACAGATAATAAAGAACAAGGTGCAGGGGATCAAGGTATTATGTTTGGTTATGCTAAAGATGATACGA
AAGAACTTTTACCTTTACCGATTTTTCTAGCACATGAATTAACTAAGCGTTTAGCTTTTGTTAGAGAAGAAGGTATTGTA
TCCGGACTTGGACCAGATGGTAAATCTCAAGTATCTGTTTTATATAATGAAAACCATGAACCACTGGAAGTTACAGCGAT
TGTATTATCTACACAACATGAAGCATCCAAAACACTTGAAAGTGTTAAAGCAGACATGATGAAACATGTTATCTTAGAAG
TCATTCCAAAACATTTAATTACAGATAACACTAAATACTATATTAATCCAACCGGTAGATTCGTCATTGGTGGACCAGTA
GGAGATAGTGGCTTAACAGGTCGTAAACTTATTGTAGACACTTACGGTGGATACTCACGCCACGGTGGTGGTGCATTTAG
TGGTAAAGATGCATCTAAAGTAGACAGATCAGCATCTTATATGGCAAGATACTTAGCAAAACAAGTCGTAGGAAGTGGTA
TTGCAAAAACTTGTGAAATCCAATTAGCTTATGCTATTGGTGTTGCAGAACCTGTAAGTTTATATGTAAACACTTTTGGT
ACATCTAAAGTAAGTAATGAGGTAATTGCTGAAACAATCTCCAAACACTTTGATTTAAGACCTAAAGCAATTATTAATTA
TCTTGGACTTACGAACCCAATTTTCAAACAAACCACACATAATGGCCATTTTGGTAAGTTAGAATCTAATTTAAGTTGGG
AAAAATTAGATAAAATTGAAATTTTCGAACAATTAGTATAA

Upstream 100 bases:

>100_bases
GTGCTCAACTGACAGGGGGCTAACCCACCAATAAGGAGATTTCATCACTTCCTTCTTGGGAAGTGTTTTTATTAAGTAAT
GGAAGAAAGAGGTAAATATT

Downstream 100 bases:

>100_bases
AATACATATATTCACTAACCATACGCAGCTTGAGAGGGTGACAGTAAGATGGACAATTTATTAATTTTTTACATTACAGC
AGGTATAGTCGTACTGATTC

Product: S-adenosylmethionine synthetase

Products: NA

Alternate protein names: AdoMet synthase; MAT; Methionine adenosyltransferase

Number of amino acids: Translated: 386; Mature: 385

Protein sequence:

>386_residues
MSYIFSSESVSKGHPDKVADFIADSVLDLVLKNDPKARCAIEVALAHTTVYIFGEVSTSYILTDEIIIETAKKAIDFCGY
NDEKFIFDAKSATYHVNIHKQSADIALGVDETDNKEQGAGDQGIMFGYAKDDTKELLPLPIFLAHELTKRLAFVREEGIV
SGLGPDGKSQVSVLYNENHEPLEVTAIVLSTQHEASKTLESVKADMMKHVILEVIPKHLITDNTKYYINPTGRFVIGGPV
GDSGLTGRKLIVDTYGGYSRHGGGAFSGKDASKVDRSASYMARYLAKQVVGSGIAKTCEIQLAYAIGVAEPVSLYVNTFG
TSKVSNEVIAETISKHFDLRPKAIINYLGLTNPIFKQTTHNGHFGKLESNLSWEKLDKIEIFEQLV

Sequences:

>Translated_386_residues
MSYIFSSESVSKGHPDKVADFIADSVLDLVLKNDPKARCAIEVALAHTTVYIFGEVSTSYILTDEIIIETAKKAIDFCGY
NDEKFIFDAKSATYHVNIHKQSADIALGVDETDNKEQGAGDQGIMFGYAKDDTKELLPLPIFLAHELTKRLAFVREEGIV
SGLGPDGKSQVSVLYNENHEPLEVTAIVLSTQHEASKTLESVKADMMKHVILEVIPKHLITDNTKYYINPTGRFVIGGPV
GDSGLTGRKLIVDTYGGYSRHGGGAFSGKDASKVDRSASYMARYLAKQVVGSGIAKTCEIQLAYAIGVAEPVSLYVNTFG
TSKVSNEVIAETISKHFDLRPKAIINYLGLTNPIFKQTTHNGHFGKLESNLSWEKLDKIEIFEQLV
>Mature_385_residues
SYIFSSESVSKGHPDKVADFIADSVLDLVLKNDPKARCAIEVALAHTTVYIFGEVSTSYILTDEIIIETAKKAIDFCGYN
DEKFIFDAKSATYHVNIHKQSADIALGVDETDNKEQGAGDQGIMFGYAKDDTKELLPLPIFLAHELTKRLAFVREEGIVS
GLGPDGKSQVSVLYNENHEPLEVTAIVLSTQHEASKTLESVKADMMKHVILEVIPKHLITDNTKYYINPTGRFVIGGPVG
DSGLTGRKLIVDTYGGYSRHGGGAFSGKDASKVDRSASYMARYLAKQVVGSGIAKTCEIQLAYAIGVAEPVSLYVNTFGT
SKVSNEVIAETISKHFDLRPKAIINYLGLTNPIFKQTTHNGHFGKLESNLSWEKLDKIEIFEQLV

Specific function: Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the e

COG id: COG0192

COG function: function code H; S-adenosylmethionine synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AdoMet synthase family

Homologues:

Organism=Homo sapiens, GI4557737, Length=376, Percent_Identity=45.2127659574468, Blast_Score=320, Evalue=2e-87,
Organism=Homo sapiens, GI5174529, Length=383, Percent_Identity=44.1253263707572, Blast_Score=312, Evalue=4e-85,
Organism=Escherichia coli, GI1789311, Length=382, Percent_Identity=50.7853403141361, Blast_Score=383, Evalue=1e-107,
Organism=Caenorhabditis elegans, GI17538498, Length=383, Percent_Identity=45.1697127937337, Blast_Score=330, Evalue=1e-90,
Organism=Caenorhabditis elegans, GI17551082, Length=388, Percent_Identity=44.0721649484536, Blast_Score=326, Evalue=1e-89,
Organism=Caenorhabditis elegans, GI17538494, Length=388, Percent_Identity=44.5876288659794, Blast_Score=325, Evalue=3e-89,
Organism=Caenorhabditis elegans, GI25145633, Length=387, Percent_Identity=44.1860465116279, Blast_Score=321, Evalue=4e-88,
Organism=Caenorhabditis elegans, GI32565905, Length=388, Percent_Identity=43.0412371134021, Blast_Score=294, Evalue=6e-80,
Organism=Caenorhabditis elegans, GI32565911, Length=336, Percent_Identity=44.6428571428571, Blast_Score=285, Evalue=3e-77,
Organism=Caenorhabditis elegans, GI25145635, Length=340, Percent_Identity=43.5294117647059, Blast_Score=275, Evalue=2e-74,
Organism=Caenorhabditis elegans, GI32565903, Length=314, Percent_Identity=46.4968152866242, Blast_Score=270, Evalue=8e-73,
Organism=Saccharomyces cerevisiae, GI6323209, Length=378, Percent_Identity=48.1481481481481, Blast_Score=340, Evalue=2e-94,
Organism=Saccharomyces cerevisiae, GI6320710, Length=383, Percent_Identity=46.7362924281984, Blast_Score=332, Evalue=4e-92,
Organism=Drosophila melanogaster, GI24580527, Length=384, Percent_Identity=44.53125, Blast_Score=307, Evalue=8e-84,
Organism=Drosophila melanogaster, GI24580525, Length=384, Percent_Identity=44.53125, Blast_Score=307, Evalue=8e-84,
Organism=Drosophila melanogaster, GI45552159, Length=384, Percent_Identity=44.0104166666667, Blast_Score=306, Evalue=1e-83,
Organism=Drosophila melanogaster, GI24580529, Length=384, Percent_Identity=44.0104166666667, Blast_Score=306, Evalue=1e-83,
Organism=Drosophila melanogaster, GI24580537, Length=384, Percent_Identity=44.0104166666667, Blast_Score=306, Evalue=1e-83,
Organism=Drosophila melanogaster, GI24580533, Length=384, Percent_Identity=44.0104166666667, Blast_Score=306, Evalue=1e-83,
Organism=Drosophila melanogaster, GI24580535, Length=384, Percent_Identity=44.0104166666667, Blast_Score=306, Evalue=1e-83,
Organism=Drosophila melanogaster, GI24580531, Length=384, Percent_Identity=44.0104166666667, Blast_Score=306, Evalue=1e-83,
Organism=Drosophila melanogaster, GI24580541, Length=370, Percent_Identity=44.0540540540541, Blast_Score=287, Evalue=9e-78,
Organism=Drosophila melanogaster, GI24580539, Length=122, Percent_Identity=36.0655737704918, Blast_Score=81, Evalue=1e-15,

Paralogues:

None

Copy number: 7,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): METK_ACHLI (A9NF20)

Other databases:

- EMBL:   CP000896
- RefSeq:   YP_001620326.1
- ProteinModelPortal:   A9NF20
- SMR:   A9NF20
- GeneID:   5804302
- GenomeReviews:   CP000896_GR
- KEGG:   acl:ACL_0328
- HOGENOM:   HBG443662
- OMA:   ENHEPLE
- BioCyc:   ALAI441768:ACL_0328-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00086
- InterPro:   IPR022631
- InterPro:   IPR022630
- InterPro:   IPR022629
- InterPro:   IPR022628
- InterPro:   IPR002133
- InterPro:   IPR022636
- PANTHER:   PTHR11964
- PIRSF:   PIRSF000497
- TIGRFAMs:   TIGR01034

Pfam domain/function: PF02773 S-AdoMet_synt_C; PF02772 S-AdoMet_synt_M; PF00438 S-AdoMet_synt_N; SSF55973 S-AdoMet_synt

EC number: =2.5.1.6

Molecular weight: Translated: 42289; Mature: 42157

Theoretical pI: Translated: 6.06; Mature: 6.06

Prosite motif: PS00376 ADOMET_SYNTHASE_1; PS00377 ADOMET_SYNTHASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSYIFSSESVSKGHPDKVADFIADSVLDLVLKNDPKARCAIEVALAHTTVYIFGEVSTSY
CCEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEEEEEEEEEEECCCEE
ILTDEIIIETAKKAIDFCGYNDEKFIFDAKSATYHVNIHKQSADIALGVDETDNKEQGAG
EEEHHHHHHHHHHHHHHCCCCCCEEEEECCCCEEEEEEEECCCCEEEECCCCCCCCCCCC
DQGIMFGYAKDDTKELLPLPIFLAHELTKRLAFVREEGIVSGLGPDGKSQVSVLYNENHE
CCCEEEEECCCCCHHHCCCHHHHHHHHHHHHHHHHHCCCEECCCCCCCCEEEEEECCCCC
PLEVTAIVLSTQHEASKTLESVKADMMKHVILEVIPKHLITDNTKYYINPTGRFVIGGPV
CEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCEEEECCC
GDSGLTGRKLIVDTYGGYSRHGGGAFSGKDASKVDRSASYMARYLAKQVVGSGIAKTCEI
CCCCCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEE
QLAYAIGVAEPVSLYVNTFGTSKVSNEVIAETISKHFDLRPKAIINYLGLTNPIFKQTTH
EEEEEECCCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHCCC
NGHFGKLESNLSWEKLDKIEIFEQLV
CCCCCCCCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
SYIFSSESVSKGHPDKVADFIADSVLDLVLKNDPKARCAIEVALAHTTVYIFGEVSTSY
CEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEEEEEEEEEEECCCEE
ILTDEIIIETAKKAIDFCGYNDEKFIFDAKSATYHVNIHKQSADIALGVDETDNKEQGAG
EEEHHHHHHHHHHHHHHCCCCCCEEEEECCCCEEEEEEEECCCCEEEECCCCCCCCCCCC
DQGIMFGYAKDDTKELLPLPIFLAHELTKRLAFVREEGIVSGLGPDGKSQVSVLYNENHE
CCCEEEEECCCCCHHHCCCHHHHHHHHHHHHHHHHHCCCEECCCCCCCCEEEEEECCCCC
PLEVTAIVLSTQHEASKTLESVKADMMKHVILEVIPKHLITDNTKYYINPTGRFVIGGPV
CEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCEEEECCC
GDSGLTGRKLIVDTYGGYSRHGGGAFSGKDASKVDRSASYMARYLAKQVVGSGIAKTCEI
CCCCCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEE
QLAYAIGVAEPVSLYVNTFGTSKVSNEVIAETISKHFDLRPKAIINYLGLTNPIFKQTTH
EEEEEECCCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHCCC
NGHFGKLESNLSWEKLDKIEIFEQLV
CCCCCCCCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA