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The map label for this gene is nusA [H]

Identifier: 162447183

GI number: 162447183

Start: 326574

End: 327650

Strand: Direct

Name: nusA [H]

Synonym: ACL_0317

Alternate gene names: 162447183

Gene position: 326574-327650 (Clockwise)

Preceding gene: 162447182

Following gene: 162447184

Centisome position: 21.82

GC content: 32.87

Gene sequence:

>1077_bases
ATGATCAGTAAAGCATTTTTCCAAAATATTGAAGAAGTCGCTGAAGATAACGACTTAAATAAAGAACAAGTATTCCATGC
TTTTGAGCAAGGATTAATTGCTGCATGTAAAAAGCAATTAGGTGTACAAACATGCCGTGTAGAATTTAAAGAAGAAAAAC
ATGAATTATTAATCTATGGTCAATATTATGTATTACCTGAAGGTGAACTTAATTTAGAACTAGATAAAAAGTATACTTTC
TTAAAATTAGAAGATGCTAAAGCATTAAAGAAATCAGCTAAAGCTGGCGAGATTTTAGAAGTAAAAATTGAACCAAGTGA
ATTTAGCTATAATGCAAGCCGTGATTTAAAACATAGATTCAATGAAGTTTTAAATCAAATTAAGAAAGAAAACATCTATC
AAGGATTAAAAGATCTTCAATATGAAATGGTAACAGCTAGAGTCTTAGAAGAAGAAAAAGACTTCTACCGTATTGAAATC
AACAAGGATGTTATTACAATGCTACCTAAAAAGGAAGCATTACCTACAGATAAATTCCATGTAGGGGATCGTATTAAAGT
TTATGTTACGGATGTAGAAATGAAGACGAAAGGTCCTAAAATTTATGTTTCTCGTACACATGTTGCTTTAGTCACTCGTC
TACTTGAAGAATATGTACCAGAAATCAAAGATGGTACGATTGAAGTTTTAGGTATCGCACGTGATCCAGGTGATCGTTCT
AAAGTAGGTTTAAAATCTAATAATGAGAACGTTGACGTTATCGGAGCTACAGTTGGTGAAGGTGGCGTACGTATTAAAGA
AATCTCTAAATTCCTATCTGGAGAAAAAATTGATTTATTCAGATGGAGTGATAATGAACAAGAACTCATTGGTAACTCTT
TACAACCGGCACCGGTTGTAGCAGTTACGAGAGTAAATCCTAAAGAAAAGAGTGCATTAGCAATTGTGCCTGATGCTCAA
TTATCTTTAGCAATTGGTAAACTTGGTCAAAATGTTAAGTTAGCAGTTCAAGCAAGTGGTTGGTCTATTGATATTAAATC
TGAGACTACAGCAGCTGAAGAAGGCATTATTTACTAA

Upstream 100 bases:

>100_bases
TAGTTTTTTCATTAAAGGGCGTCCAAAAAAACTACAAATTAAATATGAAAACATAAGATTTATCCGCCAAGCGGTTAAAT
TTTAAAAGGAGAGAATAAAA

Downstream 100 bases:

>100_bases
GAGGGTTTAAAATGAAAACAGTTAAAAAGATTCCAATGCGTACATGTGTCGTTACAAAGACTGTTCATCCTAAAAAAGAT
TTGGTACGCATTGTAGCAAA

Product: transcription elongation factor NusA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 358; Mature: 358

Protein sequence:

>358_residues
MISKAFFQNIEEVAEDNDLNKEQVFHAFEQGLIAACKKQLGVQTCRVEFKEEKHELLIYGQYYVLPEGELNLELDKKYTF
LKLEDAKALKKSAKAGEILEVKIEPSEFSYNASRDLKHRFNEVLNQIKKENIYQGLKDLQYEMVTARVLEEEKDFYRIEI
NKDVITMLPKKEALPTDKFHVGDRIKVYVTDVEMKTKGPKIYVSRTHVALVTRLLEEYVPEIKDGTIEVLGIARDPGDRS
KVGLKSNNENVDVIGATVGEGGVRIKEISKFLSGEKIDLFRWSDNEQELIGNSLQPAPVVAVTRVNPKEKSALAIVPDAQ
LSLAIGKLGQNVKLAVQASGWSIDIKSETTAAEEGIIY

Sequences:

>Translated_358_residues
MISKAFFQNIEEVAEDNDLNKEQVFHAFEQGLIAACKKQLGVQTCRVEFKEEKHELLIYGQYYVLPEGELNLELDKKYTF
LKLEDAKALKKSAKAGEILEVKIEPSEFSYNASRDLKHRFNEVLNQIKKENIYQGLKDLQYEMVTARVLEEEKDFYRIEI
NKDVITMLPKKEALPTDKFHVGDRIKVYVTDVEMKTKGPKIYVSRTHVALVTRLLEEYVPEIKDGTIEVLGIARDPGDRS
KVGLKSNNENVDVIGATVGEGGVRIKEISKFLSGEKIDLFRWSDNEQELIGNSLQPAPVVAVTRVNPKEKSALAIVPDAQ
LSLAIGKLGQNVKLAVQASGWSIDIKSETTAAEEGIIY
>Mature_358_residues
MISKAFFQNIEEVAEDNDLNKEQVFHAFEQGLIAACKKQLGVQTCRVEFKEEKHELLIYGQYYVLPEGELNLELDKKYTF
LKLEDAKALKKSAKAGEILEVKIEPSEFSYNASRDLKHRFNEVLNQIKKENIYQGLKDLQYEMVTARVLEEEKDFYRIEI
NKDVITMLPKKEALPTDKFHVGDRIKVYVTDVEMKTKGPKIYVSRTHVALVTRLLEEYVPEIKDGTIEVLGIARDPGDRS
KVGLKSNNENVDVIGATVGEGGVRIKEISKFLSGEKIDLFRWSDNEQELIGNSLQPAPVVAVTRVNPKEKSALAIVPDAQ
LSLAIGKLGQNVKLAVQASGWSIDIKSETTAAEEGIIY

Specific function: Participates in both the termination and antitermination of transcription. Interacts with RNA polymerase and binds RNA [H]

COG id: COG0195

COG function: function code K; Transcription elongation factor

Gene ontology:
GO:0003700: Transcription termination factor
GO:0003723: Transcription termination factor
GO:0006351: Transcription termination factor
GO:0031554: Transcription termination factor

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI1789560, Length=348, Percent_Identity=27.0114942528736, Blast_Score=157, Evalue=1e-39,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1612 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004087
- InterPro:   IPR015946
- InterPro:   IPR009019
- InterPro:   IPR004088
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR022967
- InterPro:   IPR013735
- InterPro:   IPR010213 [H]

Pfam domain/function: PF08529 NusA_N; PF00575 S1 [H]

EC number: NA

Molecular weight: Translated: 40376; Mature: 40376

Theoretical pI: Translated: 5.28; Mature: 5.28

Prosite motif: PS50084 KH_TYPE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISKAFFQNIEEVAEDNDLNKEQVFHAFEQGLIAACKKQLGVQTCRVEFKEEKHELLIYG
CCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCHHEEEEEECCCCCEEEEEE
QYYVLPEGELNLELDKKYTFLKLEDAKALKKSAKAGEILEVKIEPSEFSYNASRDLKHRF
EEEEECCCCEEEEECCCEEEEEECCHHHHHHCCCCCCEEEEEECCCCCCCCCCHHHHHHH
NEVLNQIKKENIYQGLKDLQYEMVTARVLEEEKDFYRIEINKDVITMLPKKEALPTDKFH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCEEECCCCCCCCCCCEE
VGDRIKVYVTDVEMKTKGPKIYVSRTHVALVTRLLEEYVPEIKDGTIEVLGIARDPGDRS
CCCEEEEEEEEEEEECCCCEEEEECHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCCC
KVGLKSNNENVDVIGATVGEGGVRIKEISKFLSGEKIDLFRWSDNEQELIGNSLQPAPVV
CCCCCCCCCCEEEEEEEECCCCEEHHHHHHHHCCCEEEEEEECCCHHHHHCCCCCCCCEE
AVTRVNPKEKSALAIVPDAQLSLAIGKLGQNVKLAVQASGWSIDIKSETTAAEEGIIY
EEEECCCCCCCEEEEECCCHHHHHHHHCCCCEEEEEEECCEEEEECCCCCCCCCCCCC
>Mature Secondary Structure
MISKAFFQNIEEVAEDNDLNKEQVFHAFEQGLIAACKKQLGVQTCRVEFKEEKHELLIYG
CCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCHHEEEEEECCCCCEEEEEE
QYYVLPEGELNLELDKKYTFLKLEDAKALKKSAKAGEILEVKIEPSEFSYNASRDLKHRF
EEEEECCCCEEEEECCCEEEEEECCHHHHHHCCCCCCEEEEEECCCCCCCCCCHHHHHHH
NEVLNQIKKENIYQGLKDLQYEMVTARVLEEEKDFYRIEINKDVITMLPKKEALPTDKFH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCEEECCCCCCCCCCCEE
VGDRIKVYVTDVEMKTKGPKIYVSRTHVALVTRLLEEYVPEIKDGTIEVLGIARDPGDRS
CCCEEEEEEEEEEEECCCCEEEEECHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCCC
KVGLKSNNENVDVIGATVGEGGVRIKEISKFLSGEKIDLFRWSDNEQELIGNSLQPAPVV
CCCCCCCCCCEEEEEEEECCCCEEHHHHHHHHCCCEEEEEEECCCHHHHHCCCCCCCCEE
AVTRVNPKEKSALAIVPDAQLSLAIGKLGQNVKLAVQASGWSIDIKSETTAAEEGIIY
EEEECCCCCCCEEEEECCCHHHHHHHHCCCCEEEEEEECCEEEEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11058132 [H]