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The map label for this gene is cobB [H]

Identifier: 162447157

GI number: 162447157

Start: 300176

End: 300886

Strand: Direct

Name: cobB [H]

Synonym: ACL_0289

Alternate gene names: 162447157

Gene position: 300176-300886 (Clockwise)

Preceding gene: 162447156

Following gene: 162447158

Centisome position: 20.05

GC content: 31.5

Gene sequence:

>711_bases
ATGACTATAAAAGAACTACAAACTATCATAGATACATCGAACTATATCGTCTTTTTTGGTGGTGCTGGAGTATCTACAGA
ATCTGGTATTAAAGACTTTAGAAGTAAAGATGGTTTATATAGCCAAAAATTTAAAAACTACCGACCAGAAGATATCCTAA
GTAGAAGTTTTTTCTTTAATCATACAAAAGATTTCTATGAGTATTTTAGACGCCACTTCTTAGATACTGGCATTAAACCA
AATAAAGCACATATGTGGCTCAAAGATGCTGAAAGTATCGGTAAAGTTAAATGTGTTATTACTCAAAATATTGATGGACT
CCATCAAATGGCAGGTTCAAAAAATGTAGTAGAACTCCACGGATCAGCTCACAGATATTACACAGTAAAAAAGAAACAAC
CTATTGATGGGTTAACCTACATTTTAAATACTGAAGATATACCAGTAGATAGTGAAGGCGATCTTATAAAGCCAGATGTT
GTACTTTATGAAGAACCACTTAACTCTGAAGTAGTAAGAAAAGCCATAGAAGAAATATCAAAGGCAGATACTTTAATTGT
TGCAGGTACATCACTAACGGTTTATCCAGCAGCAAGTTTTATAGAATACTTTAAGGGTAAACACTTAATTGTGATAAATA
AAGAGTATATGCAATTTAAACACTTTATTGAAGGTCAAGTTGGTGAAATTTTAAGTCAAATTAAGTTATAA

Upstream 100 bases:

>100_bases
TAGATTAGAAAAAACTACTTTTCATCGAGAGTAGTTTTTTTATGAATCACTAGTATATTTTATATTCTAGTGATATGATG
AGGTTAAAGGAGAACTTTTT

Downstream 100 bases:

>100_bases
GAAAATAAAAAAAGTTCTCAAGCATTTACTTGAGAACTTGTTTATTATTTACTGGAGCCGGTGAGCAGGATCGAACTGCC
GTCTAAGCCTTGGCAAGGCC

Product: hypothetical protein

Products: NA

Alternate protein names: Regulatory protein SIR2 homolog [H]

Number of amino acids: Translated: 236; Mature: 235

Protein sequence:

>236_residues
MTIKELQTIIDTSNYIVFFGGAGVSTESGIKDFRSKDGLYSQKFKNYRPEDILSRSFFFNHTKDFYEYFRRHFLDTGIKP
NKAHMWLKDAESIGKVKCVITQNIDGLHQMAGSKNVVELHGSAHRYYTVKKKQPIDGLTYILNTEDIPVDSEGDLIKPDV
VLYEEPLNSEVVRKAIEEISKADTLIVAGTSLTVYPAASFIEYFKGKHLIVINKEYMQFKHFIEGQVGEILSQIKL

Sequences:

>Translated_236_residues
MTIKELQTIIDTSNYIVFFGGAGVSTESGIKDFRSKDGLYSQKFKNYRPEDILSRSFFFNHTKDFYEYFRRHFLDTGIKP
NKAHMWLKDAESIGKVKCVITQNIDGLHQMAGSKNVVELHGSAHRYYTVKKKQPIDGLTYILNTEDIPVDSEGDLIKPDV
VLYEEPLNSEVVRKAIEEISKADTLIVAGTSLTVYPAASFIEYFKGKHLIVINKEYMQFKHFIEGQVGEILSQIKL
>Mature_235_residues
TIKELQTIIDTSNYIVFFGGAGVSTESGIKDFRSKDGLYSQKFKNYRPEDILSRSFFFNHTKDFYEYFRRHFLDTGIKPN
KAHMWLKDAESIGKVKCVITQNIDGLHQMAGSKNVVELHGSAHRYYTVKKKQPIDGLTYILNTEDIPVDSEGDLIKPDVV
LYEEPLNSEVVRKAIEEISKADTLIVAGTSLTVYPAASFIEYFKGKHLIVINKEYMQFKHFIEGQVGEILSQIKL

Specific function: Modulates the activities of several enzymes which are inactive in their acetylated form [H]

COG id: COG0846

COG function: function code K; NAD-dependent protein deacetylases, SIR2 family

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 deacetylase sirtuin-type domain [H]

Homologues:

Organism=Homo sapiens, GI300795542, Length=251, Percent_Identity=27.8884462151394, Blast_Score=101, Evalue=5e-22,
Organism=Homo sapiens, GI6912662, Length=234, Percent_Identity=31.6239316239316, Blast_Score=99, Evalue=2e-21,
Organism=Homo sapiens, GI6912664, Length=266, Percent_Identity=27.8195488721804, Blast_Score=98, Evalue=5e-21,
Organism=Homo sapiens, GI63054862, Length=213, Percent_Identity=33.3333333333333, Blast_Score=97, Evalue=9e-21,
Organism=Homo sapiens, GI6912660, Length=235, Percent_Identity=31.063829787234, Blast_Score=97, Evalue=1e-20,
Organism=Homo sapiens, GI13787215, Length=238, Percent_Identity=28.5714285714286, Blast_Score=96, Evalue=2e-20,
Organism=Homo sapiens, GI13775602, Length=211, Percent_Identity=31.2796208530806, Blast_Score=87, Evalue=1e-17,
Organism=Homo sapiens, GI7657575, Length=234, Percent_Identity=27.7777777777778, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI13775600, Length=211, Percent_Identity=31.2796208530806, Blast_Score=86, Evalue=3e-17,
Organism=Homo sapiens, GI300797705, Length=187, Percent_Identity=30.4812834224599, Blast_Score=80, Evalue=2e-15,
Organism=Escherichia coli, GI308199517, Length=210, Percent_Identity=33.3333333333333, Blast_Score=103, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI17541892, Length=215, Percent_Identity=32.093023255814, Blast_Score=97, Evalue=7e-21,
Organism=Caenorhabditis elegans, GI71990482, Length=256, Percent_Identity=31.25, Blast_Score=94, Evalue=9e-20,
Organism=Caenorhabditis elegans, GI17567771, Length=252, Percent_Identity=28.968253968254, Blast_Score=90, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI71990487, Length=258, Percent_Identity=31.0077519379845, Blast_Score=88, Evalue=3e-18,
Organism=Saccharomyces cerevisiae, GI6325242, Length=211, Percent_Identity=32.2274881516588, Blast_Score=79, Evalue=6e-16,
Organism=Drosophila melanogaster, GI17137536, Length=269, Percent_Identity=27.5092936802974, Blast_Score=88, Evalue=6e-18,
Organism=Drosophila melanogaster, GI28571445, Length=164, Percent_Identity=33.5365853658537, Blast_Score=83, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24648389, Length=210, Percent_Identity=30.952380952381, Blast_Score=82, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003000 [H]

Pfam domain/function: PF02146 SIR2 [H]

EC number: 3.5.1.- [C]

Molecular weight: Translated: 26971; Mature: 26840

Theoretical pI: Translated: 7.29; Mature: 7.29

Prosite motif: PS50305 SIRTUIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIKELQTIIDTSNYIVFFGGAGVSTESGIKDFRSKDGLYSQKFKNYRPEDILSRSFFFN
CCHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHCCCCHHHHHCCCCCHHHHHHHHHHC
HTKDFYEYFRRHFLDTGIKPNKAHMWLKDAESIGKVKCVITQNIDGLHQMAGSKNVVELH
CCHHHHHHHHHHHHHCCCCCCCCEEEECCHHCCCCEEEEEECCCCHHHHHCCCCCEEEEC
GSAHRYYTVKKKQPIDGLTYILNTEDIPVDSEGDLIKPDVVLYEEPLNSEVVRKAIEEIS
CCCEEEEEECCCCCCCCEEEEECCCCCCCCCCCCEECCCEEEEECCCCHHHHHHHHHHHH
KADTLIVAGTSLTVYPAASFIEYFKGKHLIVINKEYMQFKHFIEGQVGEILSQIKL
HCCEEEEECCCEEEHHHHHHHHHHCCCEEEEECHHHHHHHHHHCCHHHHHHHHHCC
>Mature Secondary Structure 
TIKELQTIIDTSNYIVFFGGAGVSTESGIKDFRSKDGLYSQKFKNYRPEDILSRSFFFN
CHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHCCCCHHHHHCCCCCHHHHHHHHHHC
HTKDFYEYFRRHFLDTGIKPNKAHMWLKDAESIGKVKCVITQNIDGLHQMAGSKNVVELH
CCHHHHHHHHHHHHHCCCCCCCCEEEECCHHCCCCEEEEEECCCCHHHHHCCCCCEEEEC
GSAHRYYTVKKKQPIDGLTYILNTEDIPVDSEGDLIKPDVVLYEEPLNSEVVRKAIEEIS
CCCEEEEEECCCCCCCCEEEEECCCCCCCCCCCCEECCCEEEEECCCCHHHHHHHHHHHH
KADTLIVAGTSLTVYPAASFIEYFKGKHLIVINKEYMQFKHFIEGQVGEILSQIKL
HCCEEEEECCCEEEHHHHHHHHHHCCCEEEEECHHHHHHHHHHCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11466286 [H]