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The map label for this gene is nagB

Identifier: 162447152

GI number: 162447152

Start: 295843

End: 296565

Strand: Direct

Name: nagB

Synonym: ACL_0284

Alternate gene names: 162447152

Gene position: 295843-296565 (Clockwise)

Preceding gene: 162447151

Following gene: 162447153

Centisome position: 19.76

GC content: 30.43

Gene sequence:

>723_bases
TTGAAAATAAACGTATTTGAGACAAAAGAAGAATTATATAGAGCAGTAGCTGATTTTTATATTAAGGCAATCAATGAAAA
ACCAAATATGACATTAGGTTTAGCAACAGGTACAACACCGATTCCTTTATACCAAAATCTGATCAAAGCATATCAAGATA
AATTAGTATCATTCAAAGATATTACAACATTTAACTTAGATGAATATATTGGGTTACCTAAAACACATAAAGAATCATAC
TTTAGTTTTATGAGAAATCAATTATTTAATCATGTAGATATTAATTTAGATAATACACATATTCCAAGTGGTGTATTAGA
GCCTAGTGAAGCAATAAAAGAGTTTCAAACTGCTTTAGATGCTCATCAGATAGACATCCAATTATTAGGCTTAGGATCTA
ACGGACATATCGGTTTTAATGAACCAGGTACATCATTTGAATCTACAACGCATAAAACACAACTTGCATTATCAACTATT
CAAGATAACTCAAGAATGTTTGATTCTATTGATGAGGTTCCAACTGAATCAATTACAATGGGTATTAAAGATATTATGCG
TGCATCTAAAATCGTTATGATAGCAACAGGTGCTCAAAAAGCTGATGCTGTTTACAAAATGATTCAAGGTCCAGTAGATG
AAAGTCTACCAGCATCAATTTTACAAAAACATGATGATGTAGTTATCTTCCTAGATAAAGACGCAGCATCGTTATTAAAA
TAG

Upstream 100 bases:

>100_bases
GCTGGTGATAACGTATTTGAGTTTTATAGAAAAACTGTTCAAGCTTATGACATTGAAAAACCAGGTAATGTGTACGATAG
ATTAATCAGAGGTTAACGAA

Downstream 100 bases:

>100_bases
AAAGCAGGTAAGAAATCATGGCATTAAAAAATAAATTAAAATTAGACCCAACATATGAACCAGCAATTATTTACTTTAAT
GAATTTAAAGAAGAAGTAAA

Product: glucosamine-6-phosphate deaminase

Products: NA

Alternate protein names: GlcN6P deaminase; GNPDA; Glucosamine-6-phosphate isomerase

Number of amino acids: Translated: 240; Mature: 240

Protein sequence:

>240_residues
MKINVFETKEELYRAVADFYIKAINEKPNMTLGLATGTTPIPLYQNLIKAYQDKLVSFKDITTFNLDEYIGLPKTHKESY
FSFMRNQLFNHVDINLDNTHIPSGVLEPSEAIKEFQTALDAHQIDIQLLGLGSNGHIGFNEPGTSFESTTHKTQLALSTI
QDNSRMFDSIDEVPTESITMGIKDIMRASKIVMIATGAQKADAVYKMIQGPVDESLPASILQKHDDVVIFLDKDAASLLK

Sequences:

>Translated_240_residues
MKINVFETKEELYRAVADFYIKAINEKPNMTLGLATGTTPIPLYQNLIKAYQDKLVSFKDITTFNLDEYIGLPKTHKESY
FSFMRNQLFNHVDINLDNTHIPSGVLEPSEAIKEFQTALDAHQIDIQLLGLGSNGHIGFNEPGTSFESTTHKTQLALSTI
QDNSRMFDSIDEVPTESITMGIKDIMRASKIVMIATGAQKADAVYKMIQGPVDESLPASILQKHDDVVIFLDKDAASLLK
>Mature_240_residues
MKINVFETKEELYRAVADFYIKAINEKPNMTLGLATGTTPIPLYQNLIKAYQDKLVSFKDITTFNLDEYIGLPKTHKESY
FSFMRNQLFNHVDINLDNTHIPSGVLEPSEAIKEFQTALDAHQIDIQLLGLGSNGHIGFNEPGTSFESTTHKTQLALSTI
QDNSRMFDSIDEVPTESITMGIKDIMRASKIVMIATGAQKADAVYKMIQGPVDESLPASILQKHDDVVIFLDKDAASLLK

Specific function: Catalyzes the reversible isomerization-deamination of glucosamine 6-phosphate (GlcN6P) to form fructose 6-phosphate (Fru6P) and ammonium ion

COG id: COG0363

COG function: function code G; 6-phosphogluconolactonase/Glucosamine-6-phosphate isomerase/deaminase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glucosamine/galactosamine-6-phosphate isomerase family. NagB subfamily

Homologues:

Organism=Homo sapiens, GI13027378, Length=214, Percent_Identity=42.5233644859813, Blast_Score=174, Evalue=8e-44,
Organism=Homo sapiens, GI19923881, Length=214, Percent_Identity=40.6542056074766, Blast_Score=169, Evalue=2e-42,
Organism=Escherichia coli, GI1786893, Length=248, Percent_Identity=34.2741935483871, Blast_Score=153, Evalue=8e-39,
Organism=Escherichia coli, GI1789530, Length=223, Percent_Identity=30.9417040358744, Blast_Score=117, Evalue=5e-28,
Organism=Escherichia coli, GI48994958, Length=232, Percent_Identity=26.7241379310345, Blast_Score=84, Evalue=6e-18,
Organism=Caenorhabditis elegans, GI17554876, Length=247, Percent_Identity=36.4372469635627, Blast_Score=166, Evalue=1e-41,
Organism=Drosophila melanogaster, GI24581960, Length=248, Percent_Identity=35.4838709677419, Blast_Score=167, Evalue=6e-42,
Organism=Drosophila melanogaster, GI19920764, Length=248, Percent_Identity=35.4838709677419, Blast_Score=167, Evalue=6e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NAGB_ACHLI (A9NEX8)

Other databases:

- EMBL:   CP000896
- RefSeq:   YP_001620284.1
- ProteinModelPortal:   A9NEX8
- SMR:   A9NEX8
- GeneID:   5804341
- GenomeReviews:   CP000896_GR
- KEGG:   acl:ACL_0284
- HOGENOM:   HBG725991
- OMA:   NRIIQFR
- BioCyc:   ALAI441768:ACL_0284-MONOMER
- HAMAP:   MF_01241
- InterPro:   IPR006148
- InterPro:   IPR004547
- PANTHER:   PTHR11280
- TIGRFAMs:   TIGR00502

Pfam domain/function: PF01182 Glucosamine_iso

EC number: =3.5.99.6

Molecular weight: Translated: 26786; Mature: 26786

Theoretical pI: Translated: 4.96; Mature: 4.96

Prosite motif: PS01161 GLC_GALNAC_ISOMERASE

Important sites: ACT_SITE 67-67 ACT_SITE 134-134 ACT_SITE 136-136 ACT_SITE 141-141

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKINVFETKEELYRAVADFYIKAINEKPNMTLGLATGTTPIPLYQNLIKAYQDKLVSFKD
CEEEEEHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCHHC
ITTFNLDEYIGLPKTHKESYFSFMRNQLFNHVDINLDNTHIPSGVLEPSEAIKEFQTALD
CEEECHHHHCCCCCCHHHHHHHHHHHHHHCEEECCCCCCCCCCCCCCHHHHHHHHHHHHH
AHQIDIQLLGLGSNGHIGFNEPGTSFESTTHKTQLALSTIQDNSRMFDSIDEVPTESITM
HHEEEEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHCCHHHHHH
GIKDIMRASKIVMIATGAQKADAVYKMIQGPVDESLPASILQKHDDVVIFLDKDAASLLK
HHHHHHHHCCEEEEECCCHHHHHHHHHHHCCCCCCHHHHHHHCCCCEEEEECCCHHHHCC
>Mature Secondary Structure
MKINVFETKEELYRAVADFYIKAINEKPNMTLGLATGTTPIPLYQNLIKAYQDKLVSFKD
CEEEEEHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCHHC
ITTFNLDEYIGLPKTHKESYFSFMRNQLFNHVDINLDNTHIPSGVLEPSEAIKEFQTALD
CEEECHHHHCCCCCCHHHHHHHHHHHHHHCEEECCCCCCCCCCCCCCHHHHHHHHHHHHH
AHQIDIQLLGLGSNGHIGFNEPGTSFESTTHKTQLALSTIQDNSRMFDSIDEVPTESITM
HHEEEEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHCCHHHHHH
GIKDIMRASKIVMIATGAQKADAVYKMIQGPVDESLPASILQKHDDVVIFLDKDAASLLK
HHHHHHHHCCEEEEECCCHHHHHHHHHHHCCCCCCHHHHHHHCCCCEEEEECCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA