Click here to switch to the map view.

The map label for this gene is nth [H]

Identifier: 162447100

GI number: 162447100

Start: 221679

End: 222323

Strand: Direct

Name: nth [H]

Synonym: ACL_0230

Alternate gene names: 162447100

Gene position: 221679-222323 (Clockwise)

Preceding gene: 162447099

Following gene: 162447101

Centisome position: 14.81

GC content: 33.02

Gene sequence:

>645_bases
ATGACTAAAAGTCAACGTATATTTTTCAGTCACTATTTAGAAGAGCTTTTCCCAGATGCAAAAGCAGAACTAGATTTCAC
AAATAATTTTGAACTGATTGTTGCAGTTGTACTATCGGCACAAACAACAGATATCGCGGTCAACAAAGTCACAAAGGATC
TATTTAGAAAATATCCCACTCCAAACGATCTCATGCATGCAGATGTTGATGATGTTATGGACACAATTAAAACAATTGGT
CTATATAAAACAAAATCCAAAAATATCATTGGTCTTGCTAAAAGATTAGTTGAAGATTATGATGGACTTGTACCAAGTGA
AAGAAAAGATTTAGAATCACTACCAGGTGTTGGTAGAAAGACAGCGAATGTTGTATTATCTAATGCATTTGGTATACCAG
CGCTAGCTGTAGATACGCATATCTTAAGAATTTCTAAAAGACTAGGGCTTGCAGATGAAACAGATGATGTACTCGAAGTT
GAAATGAAATTGAATAAACAATTTCCTAAAGAACTGTGGCATAAACTTCATCATCAACTTATCTTTTTTGGACGATATCA
TTGTATTGCTAGAAAACCTAATTGTGATACGTGTAAGATGCAAGATATGTGTCCACACTTTAAAAATGTAATTTCTAAAC
AATAA

Upstream 100 bases:

>100_bases
TTAAGTTTTTAGAAAGAACATTAAATAGAGAAAAATATATCGCAGAACCACTTGATGAACAAGCAGAACGCGCGATTGAT
GAAATATTTAAGGCTATCTA

Downstream 100 bases:

>100_bases
AAAAATATCAAATAACAAAATAAAAATCTACATTTTTCTTTGAGAAGATGTAGATTTTTATTATTGTAAACACTATAATG
AAGCCAAAAAGGGAGATTTT

Product: endonuclease III

Products: NA

Alternate protein names: DNA-(apurinic or apyrimidinic site) lyase [H]

Number of amino acids: Translated: 214; Mature: 213

Protein sequence:

>214_residues
MTKSQRIFFSHYLEELFPDAKAELDFTNNFELIVAVVLSAQTTDIAVNKVTKDLFRKYPTPNDLMHADVDDVMDTIKTIG
LYKTKSKNIIGLAKRLVEDYDGLVPSERKDLESLPGVGRKTANVVLSNAFGIPALAVDTHILRISKRLGLADETDDVLEV
EMKLNKQFPKELWHKLHHQLIFFGRYHCIARKPNCDTCKMQDMCPHFKNVISKQ

Sequences:

>Translated_214_residues
MTKSQRIFFSHYLEELFPDAKAELDFTNNFELIVAVVLSAQTTDIAVNKVTKDLFRKYPTPNDLMHADVDDVMDTIKTIG
LYKTKSKNIIGLAKRLVEDYDGLVPSERKDLESLPGVGRKTANVVLSNAFGIPALAVDTHILRISKRLGLADETDDVLEV
EMKLNKQFPKELWHKLHHQLIFFGRYHCIARKPNCDTCKMQDMCPHFKNVISKQ
>Mature_213_residues
TKSQRIFFSHYLEELFPDAKAELDFTNNFELIVAVVLSAQTTDIAVNKVTKDLFRKYPTPNDLMHADVDDVMDTIKTIGL
YKTKSKNIIGLAKRLVEDYDGLVPSERKDLESLPGVGRKTANVVLSNAFGIPALAVDTHILRISKRLGLADETDDVLEVE
MKLNKQFPKELWHKLHHQLIFFGRYHCIARKPNCDTCKMQDMCPHFKNVISKQ

Specific function: Has Both An Apurinic And/Or Apyrimidinic Endonuclease Activity And A DNA N-Glycosylase Activity. Incises Damaged DNA At Cytosines, Thymines And Guanines. Acts On A Damaged Strand, 5' From The Damaged Site. Required For The Repair Of Both Oxidative DNA Da

COG id: COG0177

COG function: function code L; Predicted EndoIII-related endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Nth/MutY family [H]

Homologues:

Organism=Homo sapiens, GI4505471, Length=185, Percent_Identity=29.1891891891892, Blast_Score=103, Evalue=1e-22,
Organism=Escherichia coli, GI1787920, Length=210, Percent_Identity=47.6190476190476, Blast_Score=193, Evalue=7e-51,
Organism=Escherichia coli, GI1789331, Length=179, Percent_Identity=25.1396648044693, Blast_Score=70, Evalue=9e-14,
Organism=Caenorhabditis elegans, GI17554540, Length=189, Percent_Identity=34.3915343915344, Blast_Score=102, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6324530, Length=190, Percent_Identity=27.3684210526316, Blast_Score=79, Evalue=5e-16,
Organism=Drosophila melanogaster, GI45550361, Length=197, Percent_Identity=31.4720812182741, Blast_Score=103, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011257
- InterPro:   IPR004036
- InterPro:   IPR005759
- InterPro:   IPR004035
- InterPro:   IPR003651
- InterPro:   IPR003265
- InterPro:   IPR000445
- InterPro:   IPR003583
- InterPro:   IPR023170 [H]

Pfam domain/function: PF00633 HHH; PF00730 HhH-GPD [H]

EC number: =4.2.99.18 [H]

Molecular weight: Translated: 24448; Mature: 24316

Theoretical pI: Translated: 8.00; Mature: 8.00

Prosite motif: PS01155 ENDONUCLEASE_III_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKSQRIFFSHYLEELFPDAKAELDFTNNFELIVAVVLSAQTTDIAVNKVTKDLFRKYPT
CCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCC
PNDLMHADVDDVMDTIKTIGLYKTKSKNIIGLAKRLVEDYDGLVPSERKDLESLPGVGRK
CCCCHHCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCCH
TANVVLSNAFGIPALAVDTHILRISKRLGLADETDDVLEVEMKLNKQFPKELWHKLHHQL
HHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHH
IFFGRYHCIARKPNCDTCKMQDMCPHFKNVISKQ
HHHHHHHEEECCCCCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TKSQRIFFSHYLEELFPDAKAELDFTNNFELIVAVVLSAQTTDIAVNKVTKDLFRKYPT
CCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCC
PNDLMHADVDDVMDTIKTIGLYKTKSKNIIGLAKRLVEDYDGLVPSERKDLESLPGVGRK
CCCCHHCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCCH
TANVVLSNAFGIPALAVDTHILRISKRLGLADETDDVLEVEMKLNKQFPKELWHKLHHQL
HHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHH
IFFGRYHCIARKPNCDTCKMQDMCPHFKNVISKQ
HHHHHHHEEECCCCCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7704260; 8760912; 9384377 [H]