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The map label for this gene is dnaB1 [H]

Identifier: 162447067

GI number: 162447067

Start: 189386

End: 190720

Strand: Direct

Name: dnaB1 [H]

Synonym: ACL_0197

Alternate gene names: 162447067

Gene position: 189386-190720 (Clockwise)

Preceding gene: 162447066

Following gene: 162447069

Centisome position: 12.65

GC content: 33.48

Gene sequence:

>1335_bases
ATGGCCAATAAAATTCCTAACGCAGTAGATGCTGAAAAATCAGTATTAGGGGCTATTCTTTTAGATAGTCAAACTGCACC
GAATATTTTTGATGAGATCTCCGAAAGTGATTTTTACTTAAATGCACATGGACTTATCTTTCAAGCAATGAAGGATTTGT
ATAATGATCGTAAATCGATTGACTACACATCATTAAAATCAATCTTAGAGCATAAATCTCAATTAGAAACTGTTGGTGGA
TTATCTTATATTCTTGATTTATCAGAATATACTGTATCCATTGACCATATTGATACCTATATTGAAATTGTTAAAGACTT
ATCCTTAAAAAGAGATGTTATTAGAATTACACAAGAGCTTGCTACTCAAGGTCTTACATCAGATATTAACTCTGCTGAAT
ACTTAGATAAAGTTGAAGGTGCAATCTTAAGTTTATCTCAAAGAAGAAAAGTTGGAGCATTTAAAAATATCGAAGATATT
GTTGGCGATGTCACCGAAAAATTACATCACTTACAAAATCAAAAAGGTGAAGTAACAGGTTTAAGAACTGGTTATGCTAC
ATTAGATAAATATACGAATGGGCTTCAACCAGAAGAACTTATTATCTTAGCTGCTAGACCTGCTGTTGGTAAATCAGCGT
TTGCGATGAACCTAGCTTTAAATGCTGCTAAGTATAATAAAGGCGGTAAAGCGGGCGTTGCAATTTTCTCTCTTGAAATG
AGTAATGAACAATTAGTAACCCGTATGATAGCAAGTATGTCTCATATAGAAAACTCTAAATTAAGAACAGGTTTTTTAAC
ACCACAAGAATGGCGTAACTATGAGAATATGACAGGTATTTTAAATGACTATAATATCTATTTTGATGATTCATCTTCAT
CAAATATCAATGATATTAGAGCAAAATGTCGCCGTCTTGCTCAAGAAGGTAAATTAGATTTTGTTGTGATTGACTATCTT
CAATTAATTCATGCTGATGGTAATAACAGACAAGAAGAAGTTTCTAAAATCTCTAGAGCTTTAAAACAAATGGCTAAAGA
ATTACGTGTTCCGGTACTGGCATTAAGTCAGCTTTCTCGTGATGTTGAAAAATCAACGGATAAGCGTCCAACGCTTGCGC
ACTTAAGAGAATCTGGTTCGATCGAGCAAGATGCCGATATTGTTATGTTTATTCATAGAGAAGAATACTATGCAGCATCA
AGTGATGGTGAACAAACAGGACAAACAGAAATCTTGGTGCGTAAAAACCGTCAAGGACGTATTGGTGAAATTAACTTTAT
CTTCTCACCTCAATACTCAAGATTTGATGAACAATCCAATATTGAAGAAGCTTAA

Upstream 100 bases:

>100_bases
ATGACATTGGATCTGTTGGTATTTATCCAGTAACTGTAAGTTTAGATAAAGGTGTAAAGGCAACATTTGAAGTAAATATA
ATTGAAAGAAGAGATTAGTT

Downstream 100 bases:

>100_bases
TCATTATAAAACAATAACAAAAAAACCTGGAAAATTTCCAGGTTTTTGTTTATATGCACACTATTTATTGAATGCGGAAA
ACATCCAAATGTGTTTTTCA

Product: replicative DNA helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 444; Mature: 443

Protein sequence:

>444_residues
MANKIPNAVDAEKSVLGAILLDSQTAPNIFDEISESDFYLNAHGLIFQAMKDLYNDRKSIDYTSLKSILEHKSQLETVGG
LSYILDLSEYTVSIDHIDTYIEIVKDLSLKRDVIRITQELATQGLTSDINSAEYLDKVEGAILSLSQRRKVGAFKNIEDI
VGDVTEKLHHLQNQKGEVTGLRTGYATLDKYTNGLQPEELIILAARPAVGKSAFAMNLALNAAKYNKGGKAGVAIFSLEM
SNEQLVTRMIASMSHIENSKLRTGFLTPQEWRNYENMTGILNDYNIYFDDSSSSNINDIRAKCRRLAQEGKLDFVVIDYL
QLIHADGNNRQEEVSKISRALKQMAKELRVPVLALSQLSRDVEKSTDKRPTLAHLRESGSIEQDADIVMFIHREEYYAAS
SDGEQTGQTEILVRKNRQGRIGEINFIFSPQYSRFDEQSNIEEA

Sequences:

>Translated_444_residues
MANKIPNAVDAEKSVLGAILLDSQTAPNIFDEISESDFYLNAHGLIFQAMKDLYNDRKSIDYTSLKSILEHKSQLETVGG
LSYILDLSEYTVSIDHIDTYIEIVKDLSLKRDVIRITQELATQGLTSDINSAEYLDKVEGAILSLSQRRKVGAFKNIEDI
VGDVTEKLHHLQNQKGEVTGLRTGYATLDKYTNGLQPEELIILAARPAVGKSAFAMNLALNAAKYNKGGKAGVAIFSLEM
SNEQLVTRMIASMSHIENSKLRTGFLTPQEWRNYENMTGILNDYNIYFDDSSSSNINDIRAKCRRLAQEGKLDFVVIDYL
QLIHADGNNRQEEVSKISRALKQMAKELRVPVLALSQLSRDVEKSTDKRPTLAHLRESGSIEQDADIVMFIHREEYYAAS
SDGEQTGQTEILVRKNRQGRIGEINFIFSPQYSRFDEQSNIEEA
>Mature_443_residues
ANKIPNAVDAEKSVLGAILLDSQTAPNIFDEISESDFYLNAHGLIFQAMKDLYNDRKSIDYTSLKSILEHKSQLETVGGL
SYILDLSEYTVSIDHIDTYIEIVKDLSLKRDVIRITQELATQGLTSDINSAEYLDKVEGAILSLSQRRKVGAFKNIEDIV
GDVTEKLHHLQNQKGEVTGLRTGYATLDKYTNGLQPEELIILAARPAVGKSAFAMNLALNAAKYNKGGKAGVAIFSLEMS
NEQLVTRMIASMSHIENSKLRTGFLTPQEWRNYENMTGILNDYNIYFDDSSSSNINDIRAKCRRLAQEGKLDFVVIDYLQ
LIHADGNNRQEEVSKISRALKQMAKELRVPVLALSQLSRDVEKSTDKRPTLAHLRESGSIEQDADIVMFIHREEYYAASS
DGEQTGQTEILVRKNRQGRIGEINFIFSPQYSRFDEQSNIEEA

Specific function: Participates In Initiation And Elongation During Chromosome Replication; It Exhibits DNA-Dependent ATPase Activity And Contains Distinct Active Sites For ATP Binding, DNA Binding, And Interaction With DNAc Protein, Primase, And Other Prepriming Proteins.

COG id: COG0305

COG function: function code L; Replicative DNA helicase

Gene ontology:
GO:0003677: Replicative DNA helicase
GO:0003678: Replicative DNA helicase
GO:0004386: Replicative DNA helicase
GO:0005524: Replicative DNA helicase
GO:0006260: Replicative DNA helicase
GO:0016787: Replicative DNA helicase

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 SF4 helicase domain [H]

Homologues:

Organism=Escherichia coli, GI1790486, Length=439, Percent_Identity=42.1412300683371, Blast_Score=330, Evalue=1e-91,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR007692
- InterPro:   IPR007694
- InterPro:   IPR007693
- InterPro:   IPR016136 [H]

Pfam domain/function: PF00772 DnaB; PF03796 DnaB_C [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 49888; Mature: 49757

Theoretical pI: Translated: 5.07; Mature: 5.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANKIPNAVDAEKSVLGAILLDSQTAPNIFDEISESDFYLNAHGLIFQAMKDLYNDRKSI
CCCCCCCCCCHHHHHHHHHEECCCCCCHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHCCC
DYTSLKSILEHKSQLETVGGLSYILDLSEYTVSIDHIDTYIEIVKDLSLKRDVIRITQEL
CHHHHHHHHHHHHHHHHHCCHHHHEEHHHHEEEHHHHHHHHHHHHHHHHHHHHHHHHHHH
ATQGLTSDINSAEYLDKVEGAILSLSQRRKVGAFKNIEDIVGDVTEKLHHLQNQKGEVTG
HHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCEEE
LRTGYATLDKYTNGLQPEELIILAARPAVGKSAFAMNLALNAAKYNKGGKAGVAIFSLEM
EECCHHHHHHHHCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCEEEEEEEEC
SNEQLVTRMIASMSHIENSKLRTGFLTPQEWRNYENMTGILNDYNIYFDDSSSSNINDIR
CCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHCCHHHHHCCEECCEEEECCCCCCCHHHHH
AKCRRLAQEGKLDFVVIDYLQLIHADGNNRQEEVSKISRALKQMAKELRVPVLALSQLSR
HHHHHHHHHCCCCEEHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
DVEKSTDKRPTLAHLRESGSIEQDADIVMFIHREEYYAASSDGEQTGQTEILVRKNRQGR
HHHHCCCCCCHHHHHHHCCCCCCCCCEEEEEEECHHCCCCCCCCCCCCEEEEEEECCCCC
IGEINFIFSPQYSRFDEQSNIEEA
CEEEEEEECCCHHHCCCCCCCCCC
>Mature Secondary Structure 
ANKIPNAVDAEKSVLGAILLDSQTAPNIFDEISESDFYLNAHGLIFQAMKDLYNDRKSI
CCCCCCCCCHHHHHHHHHEECCCCCCHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHCCC
DYTSLKSILEHKSQLETVGGLSYILDLSEYTVSIDHIDTYIEIVKDLSLKRDVIRITQEL
CHHHHHHHHHHHHHHHHHCCHHHHEEHHHHEEEHHHHHHHHHHHHHHHHHHHHHHHHHHH
ATQGLTSDINSAEYLDKVEGAILSLSQRRKVGAFKNIEDIVGDVTEKLHHLQNQKGEVTG
HHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCEEE
LRTGYATLDKYTNGLQPEELIILAARPAVGKSAFAMNLALNAAKYNKGGKAGVAIFSLEM
EECCHHHHHHHHCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCEEEEEEEEC
SNEQLVTRMIASMSHIENSKLRTGFLTPQEWRNYENMTGILNDYNIYFDDSSSSNINDIR
CCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHCCHHHHHCCEECCEEEECCCCCCCHHHHH
AKCRRLAQEGKLDFVVIDYLQLIHADGNNRQEEVSKISRALKQMAKELRVPVLALSQLSR
HHHHHHHHHCCCCEEHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
DVEKSTDKRPTLAHLRESGSIEQDADIVMFIHREEYYAASSDGEQTGQTEILVRKNRQGR
HHHHCCCCCCHHHHHHHCCCCCCCCCEEEEEEECHHCCCCCCCCCCCCEEEEEEECCCCC
IGEINFIFSPQYSRFDEQSNIEEA
CEEEEEEECCCHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377; 1312531 [H]