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The map label for this gene is cysS

Identifier: 162447013

GI number: 162447013

Start: 127750

End: 129054

Strand: Direct

Name: cysS

Synonym: ACL_0143

Alternate gene names: 162447013

Gene position: 127750-129054 (Clockwise)

Preceding gene: 162447012

Following gene: 162447014

Centisome position: 8.53

GC content: 31.72

Gene sequence:

>1305_bases
ATGTTAAAAATATATAACTCACTGTCAAACAAAATTGAAGAATTTAAGCCAATTCATGAAAAAAAAGTAAATATGTATGT
GTGTGGACCTACAGTCTATGATGATATACATATTGGTAATGGTAGACCCGTTGTCTTTTTTGATGTTGTTAAGCGTTACT
TACAATATCTAGATTATGGCGTTAAATACGCATCTAACATCACTGATGTGGATGATAAGATCATTGACAGAGCCCAAAAA
CTACATATAACTGAAAAAGAACTAGCTACTACTTATACCAACAATTTTTTTGAAATAGCTAAAAAAATTGGTGGATATAA
TTTTGATGTCACACCACATGCAACCAACTATATTGAAGAAATGATTAAGTTTATTGGTGAACTAATTTCTGATGGGTTTG
CATATAAGACCCAAAGTGGTGTCTATTTTAGAGTGGATAAAATCAAAGACTATGGGATATTGTCGAACCAACAGGTTAAA
GACTTAAAAACTGGAGTCCGTATTGATTTAGAAACTGATAAAGAAAAAGACTATGATTTTGCTTTATGGAAGAACACAGA
AGAAGGTATTAAATATCATGCACCTTGGGGTGATGGTCGTCCTGGGTGGCATACAGAGTGTGTTGTCATGACAAACGAAA
TCTTTGGTGGTGAAATTGATATTCACGGTGGTGGGTTTGATTTAAAATTTCCACACCATGAAAATGAAATTGCACAGTCT
GTTGCAAAACATGATCACCATCTAGCGAAGTACTGGATGCATGTTGGTAGACTGGATTTAGCTAATGAAAAGATGAGTAA
ATCATTAGGTAATGATATCAAACTTAAAGACCTTGTTCAAGTCTATAATCCAAATGCATATCGCTTAATGTTGTTAGCAC
ATCATTACCGAGCGCCAATTCAGTTTAGTGATGACTTAATTGAACAATATCAAAAGGCTTATGATAAAATATCATATACA
CTGAATAAGTGGCATTTTCATATGATATTAAACAATATCAATGAAAATGGATTAGATCATGAAAGTATGGAATACTTTGA
AGGATTTATGAACGATGATTTTGCTACACCTAGAGTTATTTCCTTAATTGATAAACTCATTAAAGACTTAAATAAAGCAT
TTAAAGTAGAAAACTACAACACGATCATACAAATACTATCCACATTAGGTATAAACCCTAATATCCTTGAAGTTTTAGAA
GATGATATTCAAAACTATAATAACTGGCAACAAGCAAGGTTAGAGAAAAATTATGAGAAGGCTGATATCTATAGAAAACC
ATTATTAGATAAAGGTTTTATCTAA

Upstream 100 bases:

>100_bases
CGATGGTCGAGTTATTAAAGTCATTGCGTGAAATCATCTAAATGCTTTGCATAAAGGGGCTATTTTTTAGTATAATACTA
TGTAAAGGAATGTTTACTAC

Downstream 100 bases:

>100_bases
TGAATGGGTTAGCACTTGCATATTTAGGTGATGCTTATTATGAACTTGAAATCAGAAAATATTTGATTACTCAAGGTATT
AGAAATGTAGGTAAACTACA

Product: cysteinyl-tRNA synthetase

Products: NA

Alternate protein names: Cysteine--tRNA ligase; CysRS

Number of amino acids: Translated: 434; Mature: 434

Protein sequence:

>434_residues
MLKIYNSLSNKIEEFKPIHEKKVNMYVCGPTVYDDIHIGNGRPVVFFDVVKRYLQYLDYGVKYASNITDVDDKIIDRAQK
LHITEKELATTYTNNFFEIAKKIGGYNFDVTPHATNYIEEMIKFIGELISDGFAYKTQSGVYFRVDKIKDYGILSNQQVK
DLKTGVRIDLETDKEKDYDFALWKNTEEGIKYHAPWGDGRPGWHTECVVMTNEIFGGEIDIHGGGFDLKFPHHENEIAQS
VAKHDHHLAKYWMHVGRLDLANEKMSKSLGNDIKLKDLVQVYNPNAYRLMLLAHHYRAPIQFSDDLIEQYQKAYDKISYT
LNKWHFHMILNNINENGLDHESMEYFEGFMNDDFATPRVISLIDKLIKDLNKAFKVENYNTIIQILSTLGINPNILEVLE
DDIQNYNNWQQARLEKNYEKADIYRKPLLDKGFI

Sequences:

>Translated_434_residues
MLKIYNSLSNKIEEFKPIHEKKVNMYVCGPTVYDDIHIGNGRPVVFFDVVKRYLQYLDYGVKYASNITDVDDKIIDRAQK
LHITEKELATTYTNNFFEIAKKIGGYNFDVTPHATNYIEEMIKFIGELISDGFAYKTQSGVYFRVDKIKDYGILSNQQVK
DLKTGVRIDLETDKEKDYDFALWKNTEEGIKYHAPWGDGRPGWHTECVVMTNEIFGGEIDIHGGGFDLKFPHHENEIAQS
VAKHDHHLAKYWMHVGRLDLANEKMSKSLGNDIKLKDLVQVYNPNAYRLMLLAHHYRAPIQFSDDLIEQYQKAYDKISYT
LNKWHFHMILNNINENGLDHESMEYFEGFMNDDFATPRVISLIDKLIKDLNKAFKVENYNTIIQILSTLGINPNILEVLE
DDIQNYNNWQQARLEKNYEKADIYRKPLLDKGFI
>Mature_434_residues
MLKIYNSLSNKIEEFKPIHEKKVNMYVCGPTVYDDIHIGNGRPVVFFDVVKRYLQYLDYGVKYASNITDVDDKIIDRAQK
LHITEKELATTYTNNFFEIAKKIGGYNFDVTPHATNYIEEMIKFIGELISDGFAYKTQSGVYFRVDKIKDYGILSNQQVK
DLKTGVRIDLETDKEKDYDFALWKNTEEGIKYHAPWGDGRPGWHTECVVMTNEIFGGEIDIHGGGFDLKFPHHENEIAQS
VAKHDHHLAKYWMHVGRLDLANEKMSKSLGNDIKLKDLVQVYNPNAYRLMLLAHHYRAPIQFSDDLIEQYQKAYDKISYT
LNKWHFHMILNNINENGLDHESMEYFEGFMNDDFATPRVISLIDKLIKDLNKAFKVENYNTIIQILSTLGINPNILEVLE
DDIQNYNNWQQARLEKNYEKADIYRKPLLDKGFI

Specific function: Unknown

COG id: COG0215

COG function: function code J; Cysteinyl-tRNA synthetase

Gene ontology:
GO:0000166: Cysteinyl-tRNA synthetase
GO:0004812: Cysteinyl-tRNA synthetase
GO:0004817: Cysteinyl-tRNA synthetase
GO:0005524: Cysteinyl-tRNA synthetase
GO:0005737: Cysteinyl-tRNA synthetase
GO:0006412: Cysteinyl-tRNA synthetase
GO:0006418: Cysteinyl-tRNA synthetase
GO:0006423: Cysteinyl-tRNA synthetase
GO:0016874: Cysteinyl-tRNA synthetase
GO:0046872: Cysteinyl-tRNA synthetase

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI13375695, Length=382, Percent_Identity=34.8167539267016, Blast_Score=216, Evalue=3e-56,
Organism=Homo sapiens, GI303304987, Length=222, Percent_Identity=36.9369369369369, Blast_Score=159, Evalue=4e-39,
Organism=Homo sapiens, GI21269877, Length=222, Percent_Identity=36.9369369369369, Blast_Score=159, Evalue=4e-39,
Organism=Homo sapiens, GI62240992, Length=222, Percent_Identity=36.9369369369369, Blast_Score=159, Evalue=5e-39,
Organism=Homo sapiens, GI10835051, Length=222, Percent_Identity=36.9369369369369, Blast_Score=159, Evalue=5e-39,
Organism=Escherichia coli, GI1786737, Length=411, Percent_Identity=39.9026763990268, Blast_Score=301, Evalue=7e-83,
Organism=Caenorhabditis elegans, GI17509795, Length=244, Percent_Identity=36.4754098360656, Blast_Score=162, Evalue=4e-40,
Organism=Caenorhabditis elegans, GI17509797, Length=244, Percent_Identity=36.4754098360656, Blast_Score=162, Evalue=4e-40,
Organism=Saccharomyces cerevisiae, GI6324082, Length=258, Percent_Identity=34.4961240310078, Blast_Score=150, Evalue=3e-37,
Organism=Drosophila melanogaster, GI19922218, Length=361, Percent_Identity=37.9501385041551, Blast_Score=225, Evalue=6e-59,
Organism=Drosophila melanogaster, GI19922364, Length=222, Percent_Identity=36.036036036036, Blast_Score=151, Evalue=7e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYC_ACHLI (A9NEI9)

Other databases:

- EMBL:   CP000896
- RefSeq:   YP_001620145.1
- ProteinModelPortal:   A9NEI9
- SMR:   A9NEI9
- GeneID:   5803546
- GenomeReviews:   CP000896_GR
- KEGG:   acl:ACL_0143
- HOGENOM:   HBG327651
- OMA:   PEEMLAG
- ProtClustDB:   PRK00260
- BioCyc:   ALAI441768:ACL_0143-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00041_B
- InterPro:   IPR015803
- InterPro:   IPR015273
- InterPro:   IPR014729
- InterPro:   IPR009080
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR10890
- PRINTS:   PR00983
- SMART:   SM00840
- TIGRFAMs:   TIGR00435

Pfam domain/function: PF01406 tRNA-synt_1e; SSF47323 tRNAsyn_1a_bind

EC number: =6.1.1.16

Molecular weight: Translated: 50682; Mature: 50682

Theoretical pI: Translated: 6.14; Mature: 6.14

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 267-267

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKIYNSLSNKIEEFKPIHEKKVNMYVCGPTVYDDIHIGNGRPVVFFDVVKRYLQYLDYG
CCCHHHHHHHHHHHHCCHHHCCCEEEEECCCEECCEEECCCCCEEEHHHHHHHHHHHHHH
VKYASNITDVDDKIIDRAQKLHITEKELATTYTNNFFEIAKKIGGYNFDVTPHATNYIEE
HHHHCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHH
MIKFIGELISDGFAYKTQSGVYFRVDKIKDYGILSNQQVKDLKTGVRIDLETDKEKDYDF
HHHHHHHHHHCCCCEEECCCEEEEEECCCCCCCCCCCHHHHHHCCCEEEEECCCCCCCCE
ALWKNTEEGIKYHAPWGDGRPGWHTECVVMTNEIFGGEIDIHGGGFDLKFPHHENEIAQS
EEECCCHHCCEEECCCCCCCCCCCCEEEEEEEECCCCEEEEECCCEEEECCCCHHHHHHH
VAKHDHHLAKYWMHVGRLDLANEKMSKSLGNDIKLKDLVQVYNPNAYRLMLLAHHYRAPI
HHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCHHHHHHHHCCCCEEEEEEHHHHCCCC
QFSDDLIEQYQKAYDKISYTLNKWHFHMILNNINENGLDHESMEYFEGFMNDDFATPRVI
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCHHHH
SLIDKLIKDLNKAFKVENYNTIIQILSTLGINPNILEVLEDDIQNYNNWQQARLEKNYEK
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHCCCHH
ADIYRKPLLDKGFI
HHHHHCCCCCCCCC
>Mature Secondary Structure
MLKIYNSLSNKIEEFKPIHEKKVNMYVCGPTVYDDIHIGNGRPVVFFDVVKRYLQYLDYG
CCCHHHHHHHHHHHHCCHHHCCCEEEEECCCEECCEEECCCCCEEEHHHHHHHHHHHHHH
VKYASNITDVDDKIIDRAQKLHITEKELATTYTNNFFEIAKKIGGYNFDVTPHATNYIEE
HHHHCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHH
MIKFIGELISDGFAYKTQSGVYFRVDKIKDYGILSNQQVKDLKTGVRIDLETDKEKDYDF
HHHHHHHHHHCCCCEEECCCEEEEEECCCCCCCCCCCHHHHHHCCCEEEEECCCCCCCCE
ALWKNTEEGIKYHAPWGDGRPGWHTECVVMTNEIFGGEIDIHGGGFDLKFPHHENEIAQS
EEECCCHHCCEEECCCCCCCCCCCCEEEEEEEECCCCEEEEECCCEEEECCCCHHHHHHH
VAKHDHHLAKYWMHVGRLDLANEKMSKSLGNDIKLKDLVQVYNPNAYRLMLLAHHYRAPI
HHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCHHHHHHHHCCCCEEEEEEHHHHCCCC
QFSDDLIEQYQKAYDKISYTLNKWHFHMILNNINENGLDHESMEYFEGFMNDDFATPRVI
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCHHHH
SLIDKLIKDLNKAFKVENYNTIIQILSTLGINPNILEVLEDDIQNYNNWQQARLEKNYEK
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHCCCHH
ADIYRKPLLDKGFI
HHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA