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The map label for this gene is gltX

Identifier: 162447009

GI number: 162447009

Start: 121336

End: 122685

Strand: Direct

Name: gltX

Synonym: ACL_0139

Alternate gene names: 162447009

Gene position: 121336-122685 (Clockwise)

Preceding gene: 162447008

Following gene: 162447010

Centisome position: 8.11

GC content: 32.89

Gene sequence:

>1350_bases
ATGAAAAAGTTTAGAGCGCGTTATGCACCATCACCTACAGGACATTTACATATTGGTAATGCAAGAACAGCATTATTCAA
TTACTTATTTGCTCGTCACCATGGTGGCGACTTCATTATAAGAATTGAAGATACCGATGTTGCAAGAAATGTTGAAGGTG
GTATTACATCTCAATTAAATAATTTAAAATGGTTAGGTATGGACTGGGATGAAGGCGTTGATGTTGGAGGTTCTTTTGGT
CCTTATAATCAATTAAGCCGTCTAGAACTGTACAAAAAGTACGCATTTGAGCTACTTGAAAAAGGTTATGCTTATAAAGA
TTTTAAAGAAGGATCAGAAGATTTTGCGATCCGTTTTAAGGTTCCAGAAAATGTATTATATGAATTTGATGACGTTATTC
GTGGTACACTGAAATTTGAATCAAAAGATGTTGAAGACTGGATTATCTTAAAAGATAATGGTATTCCTACTTATAACTTT
GCTGTTGTTATTGATGATCACTATATGGAAATTACCCACGTGTTTAGAGGTGAAGAACATATTACAAATACACCTAAACA
ACTCATGGTATATGATGCTTTAGGTTGGGAGTATCCAACATTTGGCCACATGACAATTATTGTCAATGAAGACCGTAAAA
AGTTAAGTAAAAGAGATACTAATACAATTCAATTTATTGAAGATTATAAGAACTTAGGTTTCTTACCAGAAGCGATGTTA
AACTTCTTATCATTACTGGGATGGAGTCCTAAAGATGATGAAGAAATTTTATCAAAAGAAGAACTTATCTCATTATTTGA
TGAACATAGACTAAGTGCTGCACCAAGTTACTTTGATAAGCAAAAGTTAGCTTACATCAACTCTAGATACTTAAAAGCTT
TATCTATGGACGAACTAAAGGATCTAACCAGACCATTTTTAATCAATCACGGCATTGAAATTAAAAATGAAGCATGGTTA
GAAAGTTTATTATCCATCTTAAAAGATAGATTATCCTATGGTGCTGAAATCACTAAGTACTATGATCAATTCTTCCATCA
TGATTTTGTTTTAGAACCAGCTGTTTTAGAAGAAATTAAAGAATTTGATAATGAAGTTGTTATTAAAGGCTTTATGGGTG
CAATATCGTCTGTTGACTTTACAGATGACGTGGCAATTAACCAAGCACTTAAAGATACCGGTAAAGCCCTAAATATTAAA
GGTAAACCACTATTCATGCCGATTAGAATAGCAACTACTGGCGAAGCACATGGACCAAGCCTACCGGTATCATTAAGTTT
ACTAGGTAAAGAATTAGTAATTAAACGCATGAACAAAACTTTAGAAGTACTAAAAGGAGAAACTAAATGA

Upstream 100 bases:

>100_bases
TTAGGAATAAGAAGTTAGATAAATAACAAGCATAATACATAATAAACTTGTTTATTCAGTCGAAATAATTTATAATAAAG
GTTGCGAAATGAGGGTGAAC

Downstream 100 bases:

>100_bases
AAAAAGTTTTAACAATTGTTGCTTTTGCATTATTGCTTGTACTAGCAGGTTGTACATCACAAGCAAGATCTAATGCAAGA
GCATATGTAACATCTATCTC

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase; GluRS

Number of amino acids: Translated: 449; Mature: 449

Protein sequence:

>449_residues
MKKFRARYAPSPTGHLHIGNARTALFNYLFARHHGGDFIIRIEDTDVARNVEGGITSQLNNLKWLGMDWDEGVDVGGSFG
PYNQLSRLELYKKYAFELLEKGYAYKDFKEGSEDFAIRFKVPENVLYEFDDVIRGTLKFESKDVEDWIILKDNGIPTYNF
AVVIDDHYMEITHVFRGEEHITNTPKQLMVYDALGWEYPTFGHMTIIVNEDRKKLSKRDTNTIQFIEDYKNLGFLPEAML
NFLSLLGWSPKDDEEILSKEELISLFDEHRLSAAPSYFDKQKLAYINSRYLKALSMDELKDLTRPFLINHGIEIKNEAWL
ESLLSILKDRLSYGAEITKYYDQFFHHDFVLEPAVLEEIKEFDNEVVIKGFMGAISSVDFTDDVAINQALKDTGKALNIK
GKPLFMPIRIATTGEAHGPSLPVSLSLLGKELVIKRMNKTLEVLKGETK

Sequences:

>Translated_449_residues
MKKFRARYAPSPTGHLHIGNARTALFNYLFARHHGGDFIIRIEDTDVARNVEGGITSQLNNLKWLGMDWDEGVDVGGSFG
PYNQLSRLELYKKYAFELLEKGYAYKDFKEGSEDFAIRFKVPENVLYEFDDVIRGTLKFESKDVEDWIILKDNGIPTYNF
AVVIDDHYMEITHVFRGEEHITNTPKQLMVYDALGWEYPTFGHMTIIVNEDRKKLSKRDTNTIQFIEDYKNLGFLPEAML
NFLSLLGWSPKDDEEILSKEELISLFDEHRLSAAPSYFDKQKLAYINSRYLKALSMDELKDLTRPFLINHGIEIKNEAWL
ESLLSILKDRLSYGAEITKYYDQFFHHDFVLEPAVLEEIKEFDNEVVIKGFMGAISSVDFTDDVAINQALKDTGKALNIK
GKPLFMPIRIATTGEAHGPSLPVSLSLLGKELVIKRMNKTLEVLKGETK
>Mature_449_residues
MKKFRARYAPSPTGHLHIGNARTALFNYLFARHHGGDFIIRIEDTDVARNVEGGITSQLNNLKWLGMDWDEGVDVGGSFG
PYNQLSRLELYKKYAFELLEKGYAYKDFKEGSEDFAIRFKVPENVLYEFDDVIRGTLKFESKDVEDWIILKDNGIPTYNF
AVVIDDHYMEITHVFRGEEHITNTPKQLMVYDALGWEYPTFGHMTIIVNEDRKKLSKRDTNTIQFIEDYKNLGFLPEAML
NFLSLLGWSPKDDEEILSKEELISLFDEHRLSAAPSYFDKQKLAYINSRYLKALSMDELKDLTRPFLINHGIEIKNEAWL
ESLLSILKDRLSYGAEITKYYDQFFHHDFVLEPAVLEEIKEFDNEVVIKGFMGAISSVDFTDDVAINQALKDTGKALNIK
GKPLFMPIRIATTGEAHGPSLPVSLSLLGKELVIKRMNKTLEVLKGETK

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu)

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:
GO:0000166: Glutamyl-tRNA synthetase
GO:0004812: Glutamyl-tRNA synthetase
GO:0004818: Glutamyl-tRNA synthetase
GO:0005524: Glutamyl-tRNA synthetase
GO:0005737: Glutamyl-tRNA synthetase
GO:0006412: Glutamyl-tRNA synthetase
GO:0006418: Glutamyl-tRNA synthetase
GO:0006424: Glutamyl-tRNA synthetase
GO:0016874: Glutamyl-tRNA synthetase
GO:0016876: Glutamyl-tRNA synthetase
GO:0043039: Glutamyl-tRNA synthetase

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI134288884, Length=492, Percent_Identity=31.3008130081301, Blast_Score=225, Evalue=8e-59,
Organism=Escherichia coli, GI1788743, Length=484, Percent_Identity=34.0909090909091, Blast_Score=289, Evalue=2e-79,
Organism=Escherichia coli, GI226510925, Length=313, Percent_Identity=28.4345047923323, Blast_Score=123, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI71993394, Length=494, Percent_Identity=31.1740890688259, Blast_Score=204, Evalue=1e-52,
Organism=Saccharomyces cerevisiae, GI6324540, Length=513, Percent_Identity=31.1890838206628, Blast_Score=211, Evalue=3e-55,
Organism=Drosophila melanogaster, GI24665321, Length=494, Percent_Identity=31.9838056680162, Blast_Score=223, Evalue=2e-58,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYE_ACHLI (A9NEI5)

Other databases:

- EMBL:   CP000896
- RefSeq:   YP_001620141.1
- ProteinModelPortal:   A9NEI5
- SMR:   A9NEI5
- GeneID:   5804418
- GenomeReviews:   CP000896_GR
- KEGG:   acl:ACL_0139
- HOGENOM:   HBG628189
- OMA:   MANKIRV
- BioCyc:   ALAI441768:ACL_0139-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00022_B
- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR001412
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729
- Gene3D:   G3DSA:1.10.1160.10
- Gene3D:   G3DSA:3.40.50.620
- Gene3D:   G3DSA:1.10.10.350
- PANTHER:   PTHR10119
- PRINTS:   PR00987
- TIGRFAMs:   TIGR00464

Pfam domain/function: PF00749 tRNA-synt_1c; SSF48163 tRNA-synt_bind

EC number: =6.1.1.17

Molecular weight: Translated: 51598; Mature: 51598

Theoretical pI: Translated: 5.28; Mature: 5.28

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 217-217

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKFRARYAPSPTGHLHIGNARTALFNYLFARHHGGDFIIRIEDTDVARNVEGGITSQLN
CCCCCCCCCCCCCCEEEECCHHHHHHHHHHHHCCCCEEEEEEECCHHHHCCCCCHHHHHC
NLKWLGMDWDEGVDVGGSFGPYNQLSRLELYKKYAFELLEKGYAYKDFKEGSEDFAIRFK
CCEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCCCEEEEEE
VPENVLYEFDDVIRGTLKFESKDVEDWIILKDNGIPTYNFAVVIDDHYMEITHVFRGEEH
CCHHHHHHHHHHHHHHHEECCCCCCCEEEEECCCCCEEEEEEEEECCHHHHHHHHCCCHH
ITNTPKQLMVYDALGWEYPTFGHMTIIVNEDRKKLSKRDTNTIQFIEDYKNLGFLPEAML
HCCCCHHEEEEECCCCCCCCCCCEEEEECCHHHHHHHCCCCHHHHHHHHHCCCCCHHHHH
NFLSLLGWSPKDDEEILSKEELISLFDEHRLSAAPSYFDKQKLAYINSRYLKALSMDELK
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCHHHHH
DLTRPFLINHGIEIKNEAWLESLLSILKDRLSYGAEITKYYDQFFHHDFVLEPAVLEEIK
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCHHHHHHHH
EFDNEVVIKGFMGAISSVDFTDDVAINQALKDTGKALNIKGKPLFMPIRIATTGEAHGPS
HCCCCEEHHHHHHHHCCCCCCCHHHHHHHHHHCCCEEEECCCEEEEEEEEEECCCCCCCC
LPVSLSLLGKELVIKRMNKTLEVLKGETK
CCEEHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MKKFRARYAPSPTGHLHIGNARTALFNYLFARHHGGDFIIRIEDTDVARNVEGGITSQLN
CCCCCCCCCCCCCCEEEECCHHHHHHHHHHHHCCCCEEEEEEECCHHHHCCCCCHHHHHC
NLKWLGMDWDEGVDVGGSFGPYNQLSRLELYKKYAFELLEKGYAYKDFKEGSEDFAIRFK
CCEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCCCEEEEEE
VPENVLYEFDDVIRGTLKFESKDVEDWIILKDNGIPTYNFAVVIDDHYMEITHVFRGEEH
CCHHHHHHHHHHHHHHHEECCCCCCCEEEEECCCCCEEEEEEEEECCHHHHHHHHCCCHH
ITNTPKQLMVYDALGWEYPTFGHMTIIVNEDRKKLSKRDTNTIQFIEDYKNLGFLPEAML
HCCCCHHEEEEECCCCCCCCCCCEEEEECCHHHHHHHCCCCHHHHHHHHHCCCCCHHHHH
NFLSLLGWSPKDDEEILSKEELISLFDEHRLSAAPSYFDKQKLAYINSRYLKALSMDELK
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCHHHHH
DLTRPFLINHGIEIKNEAWLESLLSILKDRLSYGAEITKYYDQFFHHDFVLEPAVLEEIK
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCHHHHHHHH
EFDNEVVIKGFMGAISSVDFTDDVAINQALKDTGKALNIKGKPLFMPIRIATTGEAHGPS
HCCCCEEHHHHHHHHCCCCCCCHHHHHHHHHHCCCEEEECCCEEEEEEEEEECCCCCCCC
LPVSLSLLGKELVIKRMNKTLEVLKGETK
CCEEHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA