Click here to switch to the map view.

The map label for this gene is metG [H]

Identifier: 162447002

GI number: 162447002

Start: 115135

End: 116685

Strand: Direct

Name: metG [H]

Synonym: ACL_0132

Alternate gene names: 162447002

Gene position: 115135-116685 (Clockwise)

Preceding gene: 162447000

Following gene: 162447003

Centisome position: 7.69

GC content: 30.95

Gene sequence:

>1551_bases
ATGGATAAGAAAAAATATTATATATCAACAGCAATTGCATATGCATCAGCAATCCCTCATATCGGTAACGTATATGAGAT
TATTTTAGCAGACGCAATCGCCCGTTTTAAGCGTTTAGAAGGTTATGATGTATATTTTCAAACGGGAACCGATGAACACG
GTCAAAAGATTGAAAATAACGCTAGAAATCAAAATTTGGAGACATCTGATTATGTCGATATGATCTCTAATGAAATTAAA
CGTATTTTTGAGTTAGTGAATGTAAAGTATGACAAATTTATACGTACAACAGATACATATCATAAAGAAGTTGTTCAAAA
TATCTTTGAAAAATTACTTGAACAAGGTGATATTTATCTAGGTAAATATGAAGGATGGTATTCTGTTGCTGATGAGGCGT
TTTTAAATAAGGCGGATATCGTAGATGGACTAGCACCTAGTGGAGATATTCCTGTATGGATGAGTGAGGATACTTACTTT
TTTAAATTAAGTAAATATCAAGATAGAATCGTTAAACATATTAAAGATAACCCATCATTTATTGAACCTGAAAATAGACG
TAATGAAGTACTACAAAGTTTCTTAAGTGAAAAACTACCAGATTTAAGTGTATCTAGAACTTCTTTTAAATGGGGGATTC
CAGTTAAAAGTAATCCAGAACATGTTATTTATGTTTGGATAGACGCTTTAAGTAACTATATTACAGGTTTAGGTTATAAT
CTAGATAGTAATAGTGATGCTATGAAAGATTTTTGGCCAGCGGACCTACATTTAATTGGTAAAGATATTATGCGTTTCCA
CGCTGTTTATTGGCCAGCACTGTTACTAGCATTAGGCTTGCCATTACCTAAAAAGATTTTTGGTCACCCATGGGTTTTAG
TAGATAAAAATAAGATGAGTAAGTCTGTTGGTAATACGCTTTATACAGATAATTTGGTTAAACATTTTGGTGTAGATGCT
CTAAGATATTATGTATTACATGAGATTCCTTTTGCAGCAGATGGTAATATAACTTATGAGTTAATGATTGAAAGAAATAA
CTCTGATTTAGCAAACACTTTAGGTAATTTAGTAAACCGTACCATTGGTATGGTGAATAAATACCAAAATGGTTTAGTTA
AAAAAGTGATAATCAACGAACCATTTGATTATAGTTTAAAAAATAAGTGTGAAGCACTACTTGATAAAGTAAGTACTTTA
ATGAATGAACTTAGAGTAGGTGATGCGATTGAAGAACTTATGATTGTTGCTAGAGCAGCAAATAAGTATATCGATCTATC
AGAACCTTGGAATTTAAATAAAAACGGCGAAACTGAAAAGTTAAGTCATGTATTATATAGTTTAGTTGAGACGATTCGCT
TCTTAGCTGTCTTCCTACAAAGTTATATGCCAGAAACTGCTTTAAAAATATTTGAGCAATTAAATATTGAAGCATCTGGA
TTAGATAGTGTTAAAACATTTGGGTTATACCCAGAACAACAAATTGGTACTGCAAAAGTATTATTTGAAAGATTTGATTT
AGAAAAGAAATTAAATGAGATTATAGGGTAA

Upstream 100 bases:

>100_bases
ATTGTATATAGAACTAAGGTGGTACCGCGGATTATTAAATGATGTTCGTCCTTAGACTAGTTTTTAGTTTAAGGATTTTT
TTATGGAAAGGAATAAAAAA

Downstream 100 bases:

>100_bases
ATAGATGAATAAACTTAAAGCTTGGATTGGTTCCACAAAGTTTCAAAGGCAAATTAAACCAGAAATAAAAAGAACGATCG
CTGTCATAGTATTTACAGTG

Product: methionyl-tRNA synthetase

Products: NA

Alternate protein names: Methionine--tRNA ligase; MetRS [H]

Number of amino acids: Translated: 516; Mature: 516

Protein sequence:

>516_residues
MDKKKYYISTAIAYASAIPHIGNVYEIILADAIARFKRLEGYDVYFQTGTDEHGQKIENNARNQNLETSDYVDMISNEIK
RIFELVNVKYDKFIRTTDTYHKEVVQNIFEKLLEQGDIYLGKYEGWYSVADEAFLNKADIVDGLAPSGDIPVWMSEDTYF
FKLSKYQDRIVKHIKDNPSFIEPENRRNEVLQSFLSEKLPDLSVSRTSFKWGIPVKSNPEHVIYVWIDALSNYITGLGYN
LDSNSDAMKDFWPADLHLIGKDIMRFHAVYWPALLLALGLPLPKKIFGHPWVLVDKNKMSKSVGNTLYTDNLVKHFGVDA
LRYYVLHEIPFAADGNITYELMIERNNSDLANTLGNLVNRTIGMVNKYQNGLVKKVIINEPFDYSLKNKCEALLDKVSTL
MNELRVGDAIEELMIVARAANKYIDLSEPWNLNKNGETEKLSHVLYSLVETIRFLAVFLQSYMPETALKIFEQLNIEASG
LDSVKTFGLYPEQQIGTAKVLFERFDLEKKLNEIIG

Sequences:

>Translated_516_residues
MDKKKYYISTAIAYASAIPHIGNVYEIILADAIARFKRLEGYDVYFQTGTDEHGQKIENNARNQNLETSDYVDMISNEIK
RIFELVNVKYDKFIRTTDTYHKEVVQNIFEKLLEQGDIYLGKYEGWYSVADEAFLNKADIVDGLAPSGDIPVWMSEDTYF
FKLSKYQDRIVKHIKDNPSFIEPENRRNEVLQSFLSEKLPDLSVSRTSFKWGIPVKSNPEHVIYVWIDALSNYITGLGYN
LDSNSDAMKDFWPADLHLIGKDIMRFHAVYWPALLLALGLPLPKKIFGHPWVLVDKNKMSKSVGNTLYTDNLVKHFGVDA
LRYYVLHEIPFAADGNITYELMIERNNSDLANTLGNLVNRTIGMVNKYQNGLVKKVIINEPFDYSLKNKCEALLDKVSTL
MNELRVGDAIEELMIVARAANKYIDLSEPWNLNKNGETEKLSHVLYSLVETIRFLAVFLQSYMPETALKIFEQLNIEASG
LDSVKTFGLYPEQQIGTAKVLFERFDLEKKLNEIIG
>Mature_516_residues
MDKKKYYISTAIAYASAIPHIGNVYEIILADAIARFKRLEGYDVYFQTGTDEHGQKIENNARNQNLETSDYVDMISNEIK
RIFELVNVKYDKFIRTTDTYHKEVVQNIFEKLLEQGDIYLGKYEGWYSVADEAFLNKADIVDGLAPSGDIPVWMSEDTYF
FKLSKYQDRIVKHIKDNPSFIEPENRRNEVLQSFLSEKLPDLSVSRTSFKWGIPVKSNPEHVIYVWIDALSNYITGLGYN
LDSNSDAMKDFWPADLHLIGKDIMRFHAVYWPALLLALGLPLPKKIFGHPWVLVDKNKMSKSVGNTLYTDNLVKHFGVDA
LRYYVLHEIPFAADGNITYELMIERNNSDLANTLGNLVNRTIGMVNKYQNGLVKKVIINEPFDYSLKNKCEALLDKVSTL
MNELRVGDAIEELMIVARAANKYIDLSEPWNLNKNGETEKLSHVLYSLVETIRFLAVFLQSYMPETALKIFEQLNIEASG
LDSVKTFGLYPEQQIGTAKVLFERFDLEKKLNEIIG

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation [H]

COG id: COG0143

COG function: function code J; Methionyl-tRNA synthetase

Gene ontology:
GO:0000166: Methionyl-tRNA synthetase
GO:0004812: Methionyl-tRNA synthetase
GO:0004825: Methionyl-tRNA synthetase
GO:0005524: Methionyl-tRNA synthetase
GO:0005737: Methionyl-tRNA synthetase
GO:0006412: Methionyl-tRNA synthetase
GO:0006418: Methionyl-tRNA synthetase
GO:0006431: Methionyl-tRNA synthetase
GO:0016874: Methionyl-tRNA synthetase

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI24308436, Length=506, Percent_Identity=36.1660079051383, Blast_Score=325, Evalue=9e-89,
Organism=Homo sapiens, GI14043022, Length=552, Percent_Identity=26.6304347826087, Blast_Score=176, Evalue=6e-44,
Organism=Escherichia coli, GI1788432, Length=546, Percent_Identity=25.2747252747253, Blast_Score=180, Evalue=2e-46,
Organism=Caenorhabditis elegans, GI212645096, Length=522, Percent_Identity=32.5670498084291, Blast_Score=276, Evalue=2e-74,
Organism=Caenorhabditis elegans, GI71992781, Length=412, Percent_Identity=31.7961165048544, Blast_Score=205, Evalue=5e-53,
Organism=Caenorhabditis elegans, GI17540794, Length=560, Percent_Identity=24.6428571428571, Blast_Score=146, Evalue=3e-35,
Organism=Saccharomyces cerevisiae, GI6321610, Length=537, Percent_Identity=31.098696461825, Blast_Score=275, Evalue=1e-74,
Organism=Saccharomyces cerevisiae, GI6321704, Length=558, Percent_Identity=25.2688172043011, Blast_Score=140, Evalue=7e-34,
Organism=Drosophila melanogaster, GI21358483, Length=505, Percent_Identity=33.2673267326733, Blast_Score=274, Evalue=1e-73,
Organism=Drosophila melanogaster, GI24655311, Length=552, Percent_Identity=27.3550724637681, Blast_Score=171, Evalue=2e-42,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR015413
- InterPro:   IPR004495
- InterPro:   IPR014758
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR014729
- InterPro:   IPR002547
- InterPro:   IPR009080
- InterPro:   IPR013155 [H]

Pfam domain/function: PF08264 Anticodon_1; PF09334 tRNA-synt_1g; PF01588 tRNA_bind [H]

EC number: =6.1.1.10 [H]

Molecular weight: Translated: 59236; Mature: 59236

Theoretical pI: Translated: 5.40; Mature: 5.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKKKYYISTAIAYASAIPHIGNVYEIILADAIARFKRLEGYDVYFQTGTDEHGQKIENN
CCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHH
ARNQNLETSDYVDMISNEIKRIFELVNVKYDKFIRTTDTYHKEVVQNIFEKLLEQGDIYL
HCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEE
GKYEGWYSVADEAFLNKADIVDGLAPSGDIPVWMSEDTYFFKLSKYQDRIVKHIKDNPSF
EECCCHHHHHHHHHHCHHHHHHCCCCCCCCCEEECCCEEEEEHHHHHHHHHHHHCCCCCC
IEPENRRNEVLQSFLSEKLPDLSVSRTSFKWGIPVKSNPEHVIYVWIDALSNYITGLGYN
CCCCHHHHHHHHHHHHHCCCCCCCCCCCEEECCCCCCCCCEEEEEEHHHHHHHHHHCCCC
LDSNSDAMKDFWPADLHLIGKDIMRFHAVYWPALLLALGLPLPKKIFGHPWVLVDKNKMS
CCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCEEEEECHHHH
KSVGNTLYTDNLVKHFGVDALRYYVLHEIPFAADGNITYELMIERNNSDLANTLGNLVNR
HHHCCHHHHHHHHHHHCHHHHHHHHHHHCCCCCCCCEEEEEEEECCCCHHHHHHHHHHHH
TIGMVNKYQNGLVKKVIINEPFDYSLKNKCEALLDKVSTLMNELRVGDAIEELMIVARAA
HHHHHHHHHCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NKYIDLSEPWNLNKNGETEKLSHVLYSLVETIRFLAVFLQSYMPETALKIFEQLNIEASG
HCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCC
LDSVKTFGLYPEQQIGTAKVLFERFDLEKKLNEIIG
CCHHHHHCCCCCHHCCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MDKKKYYISTAIAYASAIPHIGNVYEIILADAIARFKRLEGYDVYFQTGTDEHGQKIENN
CCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHH
ARNQNLETSDYVDMISNEIKRIFELVNVKYDKFIRTTDTYHKEVVQNIFEKLLEQGDIYL
HCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEE
GKYEGWYSVADEAFLNKADIVDGLAPSGDIPVWMSEDTYFFKLSKYQDRIVKHIKDNPSF
EECCCHHHHHHHHHHCHHHHHHCCCCCCCCCEEECCCEEEEEHHHHHHHHHHHHCCCCCC
IEPENRRNEVLQSFLSEKLPDLSVSRTSFKWGIPVKSNPEHVIYVWIDALSNYITGLGYN
CCCCHHHHHHHHHHHHHCCCCCCCCCCCEEECCCCCCCCCEEEEEEHHHHHHHHHHCCCC
LDSNSDAMKDFWPADLHLIGKDIMRFHAVYWPALLLALGLPLPKKIFGHPWVLVDKNKMS
CCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCEEEEECHHHH
KSVGNTLYTDNLVKHFGVDALRYYVLHEIPFAADGNITYELMIERNNSDLANTLGNLVNR
HHHCCHHHHHHHHHHHCHHHHHHHHHHHCCCCCCCCEEEEEEEECCCCHHHHHHHHHHHH
TIGMVNKYQNGLVKKVIINEPFDYSLKNKCEALLDKVSTLMNELRVGDAIEELMIVARAA
HHHHHHHHHCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NKYIDLSEPWNLNKNGETEKLSHVLYSLVETIRFLAVFLQSYMPETALKIFEQLNIEASG
HCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCC
LDSVKTFGLYPEQQIGTAKVLFERFDLEKKLNEIIG
CCHHHHHCCCCCHHCCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12552129 [H]