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The map label for this gene is mmsB [H]

Identifier: 162446952

GI number: 162446952

Start: 79873

End: 80727

Strand: Direct

Name: mmsB [H]

Synonym: ACL_0082

Alternate gene names: 162446952

Gene position: 79873-80727 (Clockwise)

Preceding gene: 162446951

Following gene: 162446953

Centisome position: 5.34

GC content: 34.97

Gene sequence:

>855_bases
ATGAATATAGGATTAATAGGAACAGGCATCATGGGAAGTCCAATTGTAAGACATCTTGCTGAAGGTGGTCATACATTAAC
GGTATACAATAGGACAAAAGAAAAAGCAGAAAAACTTTCAGATGTTGCTTATGTTGCATCAACGATAGAAGAACTAACGC
AAAAATCAGACATTATATTTATGATTGTTGGTTATCCAAAAGATGTAGAAACTGTAGCACTTGAAATATTTAAAACAGCT
AAACTAAATACAATCATTGTAGATATGACAACATCATCACCATCACTTGCAGCATACTTATATGATATGGCTAAAGAAAA
AGGCCTACACATGATGGATGCACCTGTAACTGGTGGAGAAGTTGGTGCTATAGCAGGTACACTATCTATTATGGTAGGTG
GCGATGAAACAAACTTTGACATCATCAGCCCTTTACTAGAAACCTTTGGTAAAACAATCCAGTATATGGGCAAAGCTGGT
AATGGACAACGCACCAAGTTAGCAAATCAAATCGCAATAGCAGGTGCACTTGCAGGTACAGTAGAGAGTTTATATTATGC
AAACTCACTGAATCTAGATCTTACAAAAGCATTTGAAATCTTTATGGGTGGTAGTGCATCAAGTACTCAGATGAAGACCA
ACGGTCTACATATGATTAATAAGTACTATGAACCAGGATTTTACATTAAACATTTCTTAAAGGACTTAAATTTAGCGATC
GAATCAAGCGTTAAACATTTAGAAGTTACAGTTAAAGTACGAGATATGCTAGAAGTATTAGTAGATAATCACCATGAAAA
TAAAGGTACCCAGGCACTCATACTATATTACCTAGATAATTTGGCGATTTTATAA

Upstream 100 bases:

>100_bases
GTCTATCGTTATTTGATATACTAATGTATAGACAATATATATGAAATATATACTCCATATTAAAAATATAATAACTATAA
TTAAAAAGAAGAGGTATGAG

Downstream 100 bases:

>100_bases
CAATATTCGCTTGATTATTCATTTAATAGTATGAATTTTCATAATTATGTGATACACTTAATCTATAAAATATATTTTGA
TACTCAAAGTATTTTAAGGG

Product: 3-hydroxyisobutyrate dehydrogenase

Products: 2-methyl-3-oxopropanoate; NADH; H+

Alternate protein names: NA

Number of amino acids: Translated: 284; Mature: 284

Protein sequence:

>284_residues
MNIGLIGTGIMGSPIVRHLAEGGHTLTVYNRTKEKAEKLSDVAYVASTIEELTQKSDIIFMIVGYPKDVETVALEIFKTA
KLNTIIVDMTTSSPSLAAYLYDMAKEKGLHMMDAPVTGGEVGAIAGTLSIMVGGDETNFDIISPLLETFGKTIQYMGKAG
NGQRTKLANQIAIAGALAGTVESLYYANSLNLDLTKAFEIFMGGSASSTQMKTNGLHMINKYYEPGFYIKHFLKDLNLAI
ESSVKHLEVTVKVRDMLEVLVDNHHENKGTQALILYYLDNLAIL

Sequences:

>Translated_284_residues
MNIGLIGTGIMGSPIVRHLAEGGHTLTVYNRTKEKAEKLSDVAYVASTIEELTQKSDIIFMIVGYPKDVETVALEIFKTA
KLNTIIVDMTTSSPSLAAYLYDMAKEKGLHMMDAPVTGGEVGAIAGTLSIMVGGDETNFDIISPLLETFGKTIQYMGKAG
NGQRTKLANQIAIAGALAGTVESLYYANSLNLDLTKAFEIFMGGSASSTQMKTNGLHMINKYYEPGFYIKHFLKDLNLAI
ESSVKHLEVTVKVRDMLEVLVDNHHENKGTQALILYYLDNLAIL
>Mature_284_residues
MNIGLIGTGIMGSPIVRHLAEGGHTLTVYNRTKEKAEKLSDVAYVASTIEELTQKSDIIFMIVGYPKDVETVALEIFKTA
KLNTIIVDMTTSSPSLAAYLYDMAKEKGLHMMDAPVTGGEVGAIAGTLSIMVGGDETNFDIISPLLETFGKTIQYMGKAG
NGQRTKLANQIAIAGALAGTVESLYYANSLNLDLTKAFEIFMGGSASSTQMKTNGLHMINKYYEPGFYIKHFLKDLNLAI
ESSVKHLEVTVKVRDMLEVLVDNHHENKGTQALILYYLDNLAIL

Specific function: Glyoxylate catabolism. [C]

COG id: COG2084

COG function: function code I; 3-hydroxyisobutyrate dehydrogenase and related beta-hydroxyacid dehydrogenases

Gene ontology:
GO:0004616: 3-hydroxyisobutyrate dehydrogenase
GO:0005488: 3-hydroxyisobutyrate dehydrogenase
GO:0006098: 3-hydroxyisobutyrate dehydrogenase
GO:0008442: 3-hydroxyisobutyrate dehydrogenase
GO:0016491: 3-hydroxyisobutyrate dehydrogenase
GO:0016616: 3-hydroxyisobutyrate dehydrogenase
GO:0050662: 3-hydroxyisobutyrate dehydrogenase
GO:0055114: 3-hydroxyisobutyrate dehydrogenase

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-hydroxyisobutyrate dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI23308751, Length=255, Percent_Identity=29.8039215686275, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI40556376, Length=242, Percent_Identity=28.9256198347107, Blast_Score=102, Evalue=4e-22,
Organism=Escherichia coli, GI1786719, Length=274, Percent_Identity=38.6861313868613, Blast_Score=169, Evalue=2e-43,
Organism=Escherichia coli, GI145693186, Length=284, Percent_Identity=33.4507042253521, Blast_Score=155, Evalue=3e-39,
Organism=Escherichia coli, GI1790315, Length=244, Percent_Identity=27.0491803278689, Blast_Score=80, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17557316, Length=252, Percent_Identity=27.3809523809524, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24655230, Length=249, Percent_Identity=33.3333333333333, Blast_Score=101, Evalue=7e-22,
Organism=Drosophila melanogaster, GI19922568, Length=249, Percent_Identity=33.3333333333333, Blast_Score=101, Evalue=7e-22,
Organism=Drosophila melanogaster, GI28574115, Length=243, Percent_Identity=27.1604938271605, Blast_Score=96, Evalue=4e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002204
- InterPro:   IPR015815
- InterPro:   IPR008927
- InterPro:   IPR006115
- InterPro:   IPR013328
- InterPro:   IPR016040
- InterPro:   IPR006183 [H]

Pfam domain/function: PF03446 NAD_binding_2 [H]

EC number: 1.1.1.31

Molecular weight: Translated: 30936; Mature: 30936

Theoretical pI: Translated: 5.85; Mature: 5.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIGLIGTGIMGSPIVRHLAEGGHTLTVYNRTKEKAEKLSDVAYVASTIEELTQKSDIIF
CCEEEEECCCCCHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCEEE
MIVGYPKDVETVALEIFKTAKLNTIIVDMTTSSPSLAAYLYDMAKEKGLHMMDAPVTGGE
EEECCCCCHHHHHHHHHHHCCCEEEEEEEECCCCHHHHHHHHHHHHCCCEEEECCCCCCC
VGAIAGTLSIMVGGDETNFDIISPLLETFGKTIQYMGKAGNGQRTKLANQIAIAGALAGT
HHHEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHH
VESLYYANSLNLDLTKAFEIFMGGSASSTQMKTNGLHMINKYYEPGFYIKHFLKDLNLAI
HHHHHHHCCCCCCHHHHHHHHCCCCCCCCEEECCCHHHHHHHCCCCHHHHHHHHHHHHHH
ESSVKHLEVTVKVRDMLEVLVDNHHENKGTQALILYYLDNLAIL
HCCCEEEEEEEEHHHHHHHHHCCCCCCCCCCEEEEEEECCHHCC
>Mature Secondary Structure
MNIGLIGTGIMGSPIVRHLAEGGHTLTVYNRTKEKAEKLSDVAYVASTIEELTQKSDIIF
CCEEEEECCCCCHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCEEE
MIVGYPKDVETVALEIFKTAKLNTIIVDMTTSSPSLAAYLYDMAKEKGLHMMDAPVTGGE
EEECCCCCHHHHHHHHHHHCCCEEEEEEEECCCCHHHHHHHHHHHHCCCEEEECCCCCCC
VGAIAGTLSIMVGGDETNFDIISPLLETFGKTIQYMGKAGNGQRTKLANQIAIAGALAGT
HHHEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHH
VESLYYANSLNLDLTKAFEIFMGGSASSTQMKTNGLHMINKYYEPGFYIKHFLKDLNLAI
HHHHHHHCCCCCCHHHHHHHHCCCCCCCCEEECCCHHHHHHHCCCCHHHHHHHHHHHHHH
ESSVKHLEVTVKVRDMLEVLVDNHHENKGTQALILYYLDNLAIL
HCCCEEEEEEEEHHHHHHHHHCCCCCCCCCCEEEEEEECCHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3-hydroxy-2-methylpropanoate; NAD+

Specific reaction: 3-hydroxy-2-methylpropanoate + NAD+ = 2-methyl-3-oxopropanoate + NADH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]