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The map label for this gene is dnaA

Identifier: 162446889

GI number: 162446889

Start: 289

End: 1647

Strand: Direct

Name: dnaA

Synonym: ACL_0001

Alternate gene names: 162446889

Gene position: 289-1647 (Clockwise)

Preceding gene: NA

Following gene: 162446891

Centisome position: 0.02

GC content: 31.49

Gene sequence:

>1359_bases
ATGAGTCCAAACAGCACATTATGGCAGACAATATTACAGGATTTAGAAAAACTATACAACGAGGAGACTTACAACGAGCT
ATTTCTACCAGTGACTTCTACTTTTAAAGATCAAAACGGATTACTTACAATGGTTGTAGCTAATGAGTTCTTAAAGAATC
GTATCAATAAACTATACATTGCAAAAATTAACGAACTTGCTACAAAATATTCAAGTACTCCAGTTAGATTGAAATTCGTA
TCACAAGAAGAAGTTATTGAAGAACCAGTAGCTGATCGTAAATTAACCATTGATTATCGTCAAGGGAACTTAAACTCTAC
CTATACCTTTGACTCTTTTGTTGTTGGAAAATCTAACATGTTTGCTTTTCGTATGGCGATGAAGGTTGCTGATCAACCTG
GAGCAGTAGCAAACCCTTTCTACATATTTGGTGATGTAGGTTTAGGTAAAACCCATCTTATGCAAGCAATAGGTAACTAT
ATATTAGATAATGATGTCGAAAAACGTATCTTATATGTTAAAGCTGATAACTTTATTGAAGACTTTGTATCTTTATTATC
AAGAAACAAAAATAAGACTGAAGAATTCAATGCTAAATATAAAGATATTGACGTTATATTAGTAGACGACATCCAAATTA
TGGCAAATGCTAGTAAAACTCAAATGGAATTCTTTAAGCTCTTTGACTACCTATATTTAAATAATAAACAAATCGTTATA
ACATCTGATAAACCAGCTTCACAATTAACAAATATCATGCCACGTTTAACGACACGTTTTGAAGCTGGTCTCTCTGTAGA
CATACAAATACCTGAATTAGAACATAGAATAAGTATTTTAAAGAGAAAAACAGCTACATTAGATGCCAACTTAGAGGTAA
GTGAGGATATTTTAACCTTTATAGCATCTCAATTTGCAGCAAACATTAGAGAAATGGAAGGTGCACTCATTCGTTTAATT
AGTTATGCACAGACCTTTAATCTAGAAATTACAATGAATGTTGTTGAAGAAGCACTTGGTGCTGTATTAAAAACTAAGAA
GAAAACAAATGATTTAAACGAAAATAACTACGATAAGATCCAAAGTATTGTAGCAGATTACTTCCAAGTTTCATTACCAG
ACTTAATTGGTAAGAAAAGACATGCTAAATTCACATTACCTAGACATATAGCGATGTATCTTATTAAACTTAAATACAAT
ATTCCTTATAAAACAATTGGATCTTTATTTAATGATAGAGACCACTCCACAGTATTATCTGCTTGTGAAAAAGTAGAACG
CGATATGAGGATGGATTCGAACTTAAAGTTTGCTGTTGACTCGATTGTCAAAAAAATAGATTCACCATTATTAAAGTGA

Upstream 100 bases:

>100_bases
AAGTTATCCACAAATAATGTGGAAAACTTTTTAATAAATTTGTCGTTTCTTATGCTATCATAGTTTTACATAAATTATTA
ACTACTATAGGGAGGCAGTC

Downstream 100 bases:

>100_bases
TAAATGTTTATAAAAATGATTAAATGTGGTAAAATAAATGGTAGATGAAGCGATTATTGCTAGTTTCCCACTTTCCCACA
GACACTAACAATAACAAAGA

Product: chromosomal replication initiator protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 452; Mature: 451

Protein sequence:

>452_residues
MSPNSTLWQTILQDLEKLYNEETYNELFLPVTSTFKDQNGLLTMVVANEFLKNRINKLYIAKINELATKYSSTPVRLKFV
SQEEVIEEPVADRKLTIDYRQGNLNSTYTFDSFVVGKSNMFAFRMAMKVADQPGAVANPFYIFGDVGLGKTHLMQAIGNY
ILDNDVEKRILYVKADNFIEDFVSLLSRNKNKTEEFNAKYKDIDVILVDDIQIMANASKTQMEFFKLFDYLYLNNKQIVI
TSDKPASQLTNIMPRLTTRFEAGLSVDIQIPELEHRISILKRKTATLDANLEVSEDILTFIASQFAANIREMEGALIRLI
SYAQTFNLEITMNVVEEALGAVLKTKKKTNDLNENNYDKIQSIVADYFQVSLPDLIGKKRHAKFTLPRHIAMYLIKLKYN
IPYKTIGSLFNDRDHSTVLSACEKVERDMRMDSNLKFAVDSIVKKIDSPLLK

Sequences:

>Translated_452_residues
MSPNSTLWQTILQDLEKLYNEETYNELFLPVTSTFKDQNGLLTMVVANEFLKNRINKLYIAKINELATKYSSTPVRLKFV
SQEEVIEEPVADRKLTIDYRQGNLNSTYTFDSFVVGKSNMFAFRMAMKVADQPGAVANPFYIFGDVGLGKTHLMQAIGNY
ILDNDVEKRILYVKADNFIEDFVSLLSRNKNKTEEFNAKYKDIDVILVDDIQIMANASKTQMEFFKLFDYLYLNNKQIVI
TSDKPASQLTNIMPRLTTRFEAGLSVDIQIPELEHRISILKRKTATLDANLEVSEDILTFIASQFAANIREMEGALIRLI
SYAQTFNLEITMNVVEEALGAVLKTKKKTNDLNENNYDKIQSIVADYFQVSLPDLIGKKRHAKFTLPRHIAMYLIKLKYN
IPYKTIGSLFNDRDHSTVLSACEKVERDMRMDSNLKFAVDSIVKKIDSPLLK
>Mature_451_residues
SPNSTLWQTILQDLEKLYNEETYNELFLPVTSTFKDQNGLLTMVVANEFLKNRINKLYIAKINELATKYSSTPVRLKFVS
QEEVIEEPVADRKLTIDYRQGNLNSTYTFDSFVVGKSNMFAFRMAMKVADQPGAVANPFYIFGDVGLGKTHLMQAIGNYI
LDNDVEKRILYVKADNFIEDFVSLLSRNKNKTEEFNAKYKDIDVILVDDIQIMANASKTQMEFFKLFDYLYLNNKQIVIT
SDKPASQLTNIMPRLTTRFEAGLSVDIQIPELEHRISILKRKTATLDANLEVSEDILTFIASQFAANIREMEGALIRLIS
YAQTFNLEITMNVVEEALGAVLKTKKKTNDLNENNYDKIQSIVADYFQVSLPDLIGKKRHAKFTLPRHIAMYLIKLKYNI
PYKTIGSLFNDRDHSTVLSACEKVERDMRMDSNLKFAVDSIVKKIDSPLLK

Specific function: Plays an important role in the initiation and regulation of chromosomal replication. Binds to the origin of replication; it binds specifically double-stranded DNA at a 9 bp consensus (dnaA box):5'-TTATC[CA]A[CA]A-3'. DnaA binds to ATP and to acidic phosph

COG id: COG0593

COG function: function code L; ATPase involved in DNA replication initiation

Gene ontology:
GO:0000166: Chromosomal replication initiator protein DnaA
GO:0003677: Chromosomal replication initiator protein DnaA
GO:0003688: Chromosomal replication initiator protein DnaA
GO:0005524: Chromosomal replication initiator protein DnaA
GO:0005737: Chromosomal replication initiator protein DnaA
GO:0006260: Chromosomal replication initiator protein DnaA
GO:0006270: Chromosomal replication initiator protein DnaA
GO:0006275: Chromosomal replication initiator protein DnaA
GO:0017111: Chromosomal replication initiator protein DnaA
GO:0043565: Chromosomal replication initiator protein DnaA

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dnaA family

Homologues:

Organism=Escherichia coli, GI2367267, Length=475, Percent_Identity=32.6315789473684, Blast_Score=259, Evalue=2e-70,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DNAA_ACHLI (A9NE65)

Other databases:

- EMBL:   CP000896
- RefSeq:   YP_001620021.1
- ProteinModelPortal:   A9NE65
- GeneID:   5803365
- GenomeReviews:   CP000896_GR
- KEGG:   acl:ACL_0001
- HOGENOM:   HBG695666
- OMA:   DEVAFLI
- ProtClustDB:   CLSK343569
- BioCyc:   ALAI441768:ACL_0001-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00377
- InterPro:   IPR003593
- InterPro:   IPR001957
- InterPro:   IPR020591
- InterPro:   IPR018312
- InterPro:   IPR013159
- InterPro:   IPR013317
- InterPro:   IPR010921
- Gene3D:   G3DSA:1.10.1750.10
- PRINTS:   PR00051
- SMART:   SM00382
- SMART:   SM00760
- TIGRFAMs:   TIGR00362

Pfam domain/function: PF00308 Bac_DnaA; PF08299 Bac_DnaA_C; SSF48295 Trp_repress_rep

EC number: NA

Molecular weight: Translated: 51805; Mature: 51674

Theoretical pI: Translated: 7.08; Mature: 7.08

Prosite motif: PS01008 DNAA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPNSTLWQTILQDLEKLYNEETYNELFLPVTSTFKDQNGLLTMVVANEFLKNRINKLYI
CCCCHHHHHHHHHHHHHHHCCCCHHHEEEEHHHCCCCCCCEEEHHHHHHHHHHHHCEEEE
AKINELATKYSSTPVRLKFVSQEEVIEEPVADRKLTIDYRQGNLNSTYTFDSFVVGKSNM
HHHHHHHHHHCCCCEEEEECCHHHHHHCCCCCCEEEEEEECCCCCCEEEECEEEECCCCH
FAFRMAMKVADQPGAVANPFYIFGDVGLGKTHLMQAIGNYILDNDVEKRILYVKADNFIE
HHHHHHHHHCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCCCEEEEEECCHHHH
DFVSLLSRNKNKTEEFNAKYKDIDVILVDDIQIMANASKTQMEFFKLFDYLYLNNKQIVI
HHHHHHHCCCCCCHHHCCCCCCEEEEEECCCCEECCCCHHHHHHHHHHHHHEECCCEEEE
TSDKPASQLTNIMPRLTTRFEAGLSVDIQIPELEHRISILKRKTATLDANLEVSEDILTF
ECCCCHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHEECCCCCHHHHHHHH
IASQFAANIREMEGALIRLISYAQTFNLEITMNVVEEALGAVLKTKKKTNDLNENNYDKI
HHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEHHHHHHHHHHHHHHHHHCCCCCCCCHHHH
QSIVADYFQVSLPDLIGKKRHAKFTLPRHIAMYLIKLKYNIPYKTIGSLFNDRDHSTVLS
HHHHHHHHHHHCHHHHCCCCCCCCCCHHHHHHHHHEEEECCCHHHHHHHHCCCCHHHHHH
ACEKVERDMRMDSNLKFAVDSIVKKIDSPLLK
HHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
SPNSTLWQTILQDLEKLYNEETYNELFLPVTSTFKDQNGLLTMVVANEFLKNRINKLYI
CCCHHHHHHHHHHHHHHHCCCCHHHEEEEHHHCCCCCCCEEEHHHHHHHHHHHHCEEEE
AKINELATKYSSTPVRLKFVSQEEVIEEPVADRKLTIDYRQGNLNSTYTFDSFVVGKSNM
HHHHHHHHHHCCCCEEEEECCHHHHHHCCCCCCEEEEEEECCCCCCEEEECEEEECCCCH
FAFRMAMKVADQPGAVANPFYIFGDVGLGKTHLMQAIGNYILDNDVEKRILYVKADNFIE
HHHHHHHHHCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCCCEEEEEECCHHHH
DFVSLLSRNKNKTEEFNAKYKDIDVILVDDIQIMANASKTQMEFFKLFDYLYLNNKQIVI
HHHHHHHCCCCCCHHHCCCCCCEEEEEECCCCEECCCCHHHHHHHHHHHHHEECCCEEEE
TSDKPASQLTNIMPRLTTRFEAGLSVDIQIPELEHRISILKRKTATLDANLEVSEDILTF
ECCCCHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHEECCCCCHHHHHHHH
IASQFAANIREMEGALIRLISYAQTFNLEITMNVVEEALGAVLKTKKKTNDLNENNYDKI
HHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEHHHHHHHHHHHHHHHHHCCCCCCCCHHHH
QSIVADYFQVSLPDLIGKKRHAKFTLPRHIAMYLIKLKYNIPYKTIGSLFNDRDHSTVLS
HHHHHHHHHHHCHHHHCCCCCCCCCCHHHHHHHHHEEEECCCHHHHHHHHCCCCHHHHHH
ACEKVERDMRMDSNLKFAVDSIVKKIDSPLLK
HHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA