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The map label for this gene is miaB [H]

Identifier: 162421780

GI number: 162421780

Start: 1897988

End: 1899436

Strand: Direct

Name: miaB [H]

Synonym: YpAngola_A1829

Alternate gene names: 162421780

Gene position: 1897988-1899436 (Clockwise)

Preceding gene: 162419030

Following gene: 162419362

Centisome position: 42.14

GC content: 48.1

Gene sequence:

>1449_bases
GTGATTTTGATGATGAATAACCCGATGACTAAAAAACTGCATATCAAAACCTGGGGTTGCCAGATGAATGAATACGATTC
ATCTAAAATGGCAGATCTCTTGGCCAGTACCCATGGCTACCAGTTAACCACCATCCCGGAAGAAGCGGATTTACTGCTGC
TAAATACCTGTTCTATCCGGGAAAAAGCACAGGAAAAAGTATTCAGCCTGCTAGGGCAATGGAAATTATTGAAAGAGAAA
AACCCGCAGTTGATTATTGGGGTCGGTGGTTGCGTGGCGTCACAGGAAGGTGAACAATTGCGCCAGCGCGCCCCCTGTGT
CGATGTCATCTTCGGGCCACAAACGCTGCATCGTTTACCGGAAATGATTAACCACGTGCAAGGAACGAATAGCCCTGTCG
TGGATATCAGTTTTCCCGAAATTGAAAAATTTGACCGTTTGCCAGAACCACGGGCTGAAGGCCCAACTGCGTTTGTTTCG
ATCATGGAAGGCTGCAATAAATATTGTACATTCTGTGTCGTACCCTACACCCGAGGCGAGGAAGTCAGCCGTCCAAGCGA
TGACATTCTTTTTGAAATCGCCCAGTTAGCCGCTCAAGGCGTACGTGAAGTCAACTTACTCGGCCAGAATGTTAACGCCT
ACCGCGGGGCAACCTACGATGGCGATATCTGTAGTTTTGCCGAGCTGTTGCGTTTGGTTGCGGCGATTGATGGTATCGAT
AGGGTACGATTTACCACCAGCCACCCCATTGAGTTTACTGACGATATCATTGATGTTTATCGCGATACGCCTGAATTGGT
GAGCTTCCTGCATTTGCCGGTACAAAGCGGTTCTGACCGTATTTTGACCATGATGAAGCGTGCCCATACCGCCTTGGAAT
ACAAAGCCATCATCCGCAAACTGCGTCAAGCACGCCCTGATATCCAGATAAGTTCTGATTTTATTGTCGGCTTCCCTGGT
GAAACCCAGCAGGATTTTGAACAGACCATGAAACTGGTCGCAGATATTCATTTTGATACCAGCTACAGCTTTATTTACTC
CCCGCGTCCCGGCACACCAGCGGCTGATTTACCCGACAATGTGTCCGAAGAGGAGAAAAAACAGCGGCTACATATCCTGC
AACAACGCATTAGCCAACAGGCGATGGAAATTAGCCGTAAGATGGTAGGGACAGTACAGCGTGTTTTGGTGGAGGGTACC
TCTCGCAAAAATGTCATGGAACTGGCCGGGCGTACGGAAAACAACCGCGTCGTGAACTTTGAGGGTTCACCCGACATGAT
TGGTAAATTCGTTGATGTCGAAATTGTTAACGTTTATGCCAGTTCACTGCGCGGCATTTTACTGCGTACAGAAGATCAGA
TGGACCTGCGAACCCACGAGTCACCGCAATCGGTGATTGCTCGCACCCGTAAAGAAAATGAAATTGGTGTAGGTATTTAT
CAGCCTTAA

Upstream 100 bases:

>100_bases
GCAGAGGCAAATGACTACAATGCTCCCTAACGACGAAGCCTTTGCGACTCTCTACTACAACGGAGATTATCTACCACAAC
GGAGTACCGCACGGAACGAG

Downstream 100 bases:

>100_bases
CCAACATCCATTTTGGTAACGCCCGGCCTAACATCATTGATCTGACAGGTTTACTTGATCCCGCCCGCGTAACGAGGCAC
TGCATTGTCAGTGCCTGTTT

Product: (dimethylallyl)adenosine tRNA methylthiotransferase

Products: NA

Alternate protein names: tRNA-i(6)A37 methylthiotransferase [H]

Number of amino acids: Translated: 482; Mature: 482

Protein sequence:

>482_residues
MILMMNNPMTKKLHIKTWGCQMNEYDSSKMADLLASTHGYQLTTIPEEADLLLLNTCSIREKAQEKVFSLLGQWKLLKEK
NPQLIIGVGGCVASQEGEQLRQRAPCVDVIFGPQTLHRLPEMINHVQGTNSPVVDISFPEIEKFDRLPEPRAEGPTAFVS
IMEGCNKYCTFCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAYRGATYDGDICSFAELLRLVAAIDGID
RVRFTTSHPIEFTDDIIDVYRDTPELVSFLHLPVQSGSDRILTMMKRAHTALEYKAIIRKLRQARPDIQISSDFIVGFPG
ETQQDFEQTMKLVADIHFDTSYSFIYSPRPGTPAADLPDNVSEEEKKQRLHILQQRISQQAMEISRKMVGTVQRVLVEGT
SRKNVMELAGRTENNRVVNFEGSPDMIGKFVDVEIVNVYASSLRGILLRTEDQMDLRTHESPQSVIARTRKENEIGVGIY
QP

Sequences:

>Translated_482_residues
MILMMNNPMTKKLHIKTWGCQMNEYDSSKMADLLASTHGYQLTTIPEEADLLLLNTCSIREKAQEKVFSLLGQWKLLKEK
NPQLIIGVGGCVASQEGEQLRQRAPCVDVIFGPQTLHRLPEMINHVQGTNSPVVDISFPEIEKFDRLPEPRAEGPTAFVS
IMEGCNKYCTFCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAYRGATYDGDICSFAELLRLVAAIDGID
RVRFTTSHPIEFTDDIIDVYRDTPELVSFLHLPVQSGSDRILTMMKRAHTALEYKAIIRKLRQARPDIQISSDFIVGFPG
ETQQDFEQTMKLVADIHFDTSYSFIYSPRPGTPAADLPDNVSEEEKKQRLHILQQRISQQAMEISRKMVGTVQRVLVEGT
SRKNVMELAGRTENNRVVNFEGSPDMIGKFVDVEIVNVYASSLRGILLRTEDQMDLRTHESPQSVIARTRKENEIGVGIY
QP
>Mature_482_residues
MILMMNNPMTKKLHIKTWGCQMNEYDSSKMADLLASTHGYQLTTIPEEADLLLLNTCSIREKAQEKVFSLLGQWKLLKEK
NPQLIIGVGGCVASQEGEQLRQRAPCVDVIFGPQTLHRLPEMINHVQGTNSPVVDISFPEIEKFDRLPEPRAEGPTAFVS
IMEGCNKYCTFCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAYRGATYDGDICSFAELLRLVAAIDGID
RVRFTTSHPIEFTDDIIDVYRDTPELVSFLHLPVQSGSDRILTMMKRAHTALEYKAIIRKLRQARPDIQISSDFIVGFPG
ETQQDFEQTMKLVADIHFDTSYSFIYSPRPGTPAADLPDNVSEEEKKQRLHILQQRISQQAMEISRKMVGTVQRVLVEGT
SRKNVMELAGRTENNRVVNFEGSPDMIGKFVDVEIVNVYASSLRGILLRTEDQMDLRTHESPQSVIARTRKENEIGVGIY
QP

Specific function: Catalyzes the methylthiolation of N6- (dimethylallyl)adenosine (i(6)A), leading to the formation of 2- methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine [H]

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Homo sapiens, GI28872784, Length=482, Percent_Identity=35.8921161825726, Blast_Score=284, Evalue=1e-76,
Organism=Homo sapiens, GI28872782, Length=492, Percent_Identity=35.7723577235772, Blast_Score=284, Evalue=1e-76,
Organism=Homo sapiens, GI93277076, Length=446, Percent_Identity=29.8206278026906, Blast_Score=180, Evalue=3e-45,
Organism=Escherichia coli, GI1786882, Length=474, Percent_Identity=83.9662447257384, Blast_Score=842, Evalue=0.0,
Organism=Escherichia coli, GI1787057, Length=404, Percent_Identity=30.4455445544554, Blast_Score=174, Evalue=1e-44,
Organism=Caenorhabditis elegans, GI17553146, Length=463, Percent_Identity=32.6133909287257, Blast_Score=238, Evalue=6e-63,
Organism=Caenorhabditis elegans, GI71996771, Length=256, Percent_Identity=31.25, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI21356207, Length=441, Percent_Identity=36.281179138322, Blast_Score=283, Evalue=1e-76,
Organism=Drosophila melanogaster, GI19922432, Length=472, Percent_Identity=29.8728813559322, Blast_Score=171, Evalue=1e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR006463
- InterPro:   IPR007197
- InterPro:   IPR002792 [H]

Pfam domain/function: PF04055 Radical_SAM; PF01938 TRAM; PF00919 UPF0004 [H]

EC number: NA

Molecular weight: Translated: 54352; Mature: 54352

Theoretical pI: Translated: 5.39; Mature: 5.39

Prosite motif: PS50926 TRAM ; PS01278 UPF0004

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILMMNNPMTKKLHIKTWGCQMNEYDSSKMADLLASTHGYQLTTIPEEADLLLLNTCSIR
CEEEECCCCCCEEEEEEECCCCCCCCHHHHHHHHHHCCCEEEEECCCCCCEEEEECHHHH
EKAQEKVFSLLGQWKLLKEKNPQLIIGVGGCVASQEGEQLRQRAPCVDVIFGPQTLHRLP
HHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHCCCHHHHHHHHCCEEEEEECHHHHHHHH
EMINHVQGTNSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKYCTFCVVPYTRGE
HHHHHHCCCCCCEEEECCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHEEEEEEECCCCC
EVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAYRGATYDGDICSFAELLRLVAAIDGID
CCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCCCCCCCHHHHHHHHHHHHHHCCCH
RVRFTTSHPIEFTDDIIDVYRDTPELVSFLHLPVQSGSDRILTMMKRAHTALEYKAIIRK
HEEEECCCCCCHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
LRQARPDIQISSDFIVGFPGETQQDFEQTMKLVADIHFDTSYSFIYSPRPGTPAADLPDN
HHHCCCCCEECCCEEEECCCCCHHHHHHHHHHHHHHEECCCCEEEECCCCCCCCCCCCCC
VSEEEKKQRLHILQQRISQQAMEISRKMVGTVQRVLVEGTSRKNVMELAGRTENNRVVNF
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCEEEEE
EGSPDMIGKFVDVEIVNVYASSLRGILLRTEDQMDLRTHESPQSVIARTRKENEIGVGIY
CCCHHHHHHHHHHHHHHHHHHHHCEEEEECCCCCCCCCCCCHHHHHHHHCCCCCCCCCCC
QP
CC
>Mature Secondary Structure
MILMMNNPMTKKLHIKTWGCQMNEYDSSKMADLLASTHGYQLTTIPEEADLLLLNTCSIR
CEEEECCCCCCEEEEEEECCCCCCCCHHHHHHHHHHCCCEEEEECCCCCCEEEEECHHHH
EKAQEKVFSLLGQWKLLKEKNPQLIIGVGGCVASQEGEQLRQRAPCVDVIFGPQTLHRLP
HHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHCCCHHHHHHHHCCEEEEEECHHHHHHHH
EMINHVQGTNSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKYCTFCVVPYTRGE
HHHHHHCCCCCCEEEECCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHEEEEEEECCCCC
EVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAYRGATYDGDICSFAELLRLVAAIDGID
CCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCCCCCCCHHHHHHHHHHHHHHCCCH
RVRFTTSHPIEFTDDIIDVYRDTPELVSFLHLPVQSGSDRILTMMKRAHTALEYKAIIRK
HEEEECCCCCCHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
LRQARPDIQISSDFIVGFPGETQQDFEQTMKLVADIHFDTSYSFIYSPRPGTPAADLPDN
HHHCCCCCEECCCEEEECCCCCHHHHHHHHHHHHHHEECCCCEEEECCCCCCCCCCCCCC
VSEEEKKQRLHILQQRISQQAMEISRKMVGTVQRVLVEGTSRKNVMELAGRTENNRVVNF
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCEEEEE
EGSPDMIGKFVDVEIVNVYASSLRGILLRTEDQMDLRTHESPQSVIARTRKENEIGVGIY
CCCHHHHHHHHHHHHHHHHHHHHCEEEEECCCCCCCCCCCCHHHHHHHHCCCCCCCCCCC
QP
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA