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The map label for this gene is ptsN [H]

Identifier: 162421096

GI number: 162421096

Start: 1193328

End: 1193822

Strand: Direct

Name: ptsN [H]

Synonym: YpAngola_A1155

Alternate gene names: 162421096

Gene position: 1193328-1193822 (Clockwise)

Preceding gene: 162418332

Following gene: 162421539

Centisome position: 26.49

GC content: 45.66

Gene sequence:

>495_bases
GTGAAAGATGAGATGACCAACGATCCAGCATTGCAATTAAGCTCGGTACTCAATATCGAGAGCACCAAAAGCTCCGTACA
CTGCTCAAGTAAAAAACGGGCTTTGGAAATTATCAGCGAGTTAGCTGCCAAGCAGCTTAATCTACCTTCACAGGTCGTTT
TTGATGCAGTATTGACCCGTGAACGCATGGGTAGCACCGGGATTGGCAGTGGTATCGCAATACCTCATGGCAAGTTGGAA
GAGGATACACTGCGCGCGGTGGGTGTATTTATTCGTCTGGAACAGCCTATTGCCTTTGATGCGATTGATAACCAACCCGT
TGACCTGTTGTTTGCCTTGTTGGTTCCAGCAGACCAATGTAAAACTCACTTACATACTTTATCTTTAGTCGCCAAACGAT
TAGCTGATAAAACAGTGTGTCGCCGCCTGCGGTCAGCACAAAGTGATGATGAGCTTTATCAGATTATTACTGAATTACCG
CCAGAAATAGCCTAG

Upstream 100 bases:

>100_bases
CTTGTTGCTGTTATTACTCAGCAAAGGTTCTACACTCGCTACATAGCCAGATACCGGTGGATATTTATTCCCACCGGTAT
CTGGCGTAAACAGCGCTTAA

Downstream 100 bases:

>100_bases
TTTGGGGCCAATACGCCCAATAGACTTCAATGTGTAGGAAGGCGGTAAAAATAGCGTAAGACAGTAAAAATGTAGGAAGA
CAGGAAAATAGCGTAAGGCA

Product: PTS system transporter subunit IIA-like nitrogen-regulatory protein PtsN

Products: protein histidine; sugar phosphate

Alternate protein names: Enzyme IIA-NTR; Phosphotransferase enzyme IIA component; PTS system EIIA component [H]

Number of amino acids: Translated: 164; Mature: 164

Protein sequence:

>164_residues
MKDEMTNDPALQLSSVLNIESTKSSVHCSSKKRALEIISELAAKQLNLPSQVVFDAVLTRERMGSTGIGSGIAIPHGKLE
EDTLRAVGVFIRLEQPIAFDAIDNQPVDLLFALLVPADQCKTHLHTLSLVAKRLADKTVCRRLRSAQSDDELYQIITELP
PEIA

Sequences:

>Translated_164_residues
MKDEMTNDPALQLSSVLNIESTKSSVHCSSKKRALEIISELAAKQLNLPSQVVFDAVLTRERMGSTGIGSGIAIPHGKLE
EDTLRAVGVFIRLEQPIAFDAIDNQPVDLLFALLVPADQCKTHLHTLSLVAKRLADKTVCRRLRSAQSDDELYQIITELP
PEIA
>Mature_164_residues
MKDEMTNDPALQLSSVLNIESTKSSVHCSSKKRALEIISELAAKQLNLPSQVVFDAVLTRERMGSTGIGSGIAIPHGKLE
EDTLRAVGVFIRLEQPIAFDAIDNQPVDLLFALLVPADQCKTHLHTLSLVAKRLADKTVCRRLRSAQSDDELYQIITELP
PEIA

Specific function: Seems to have a role in regulating nitrogen assimilation [H]

COG id: COG1762

COG function: function code GT; Phosphotransferase system mannitol/fructose-specific IIA domain (Ntr-type)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIA type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1789597, Length=152, Percent_Identity=86.1842105263158, Blast_Score=270, Evalue=3e-74,
Organism=Escherichia coli, GI1786951, Length=127, Percent_Identity=31.496062992126, Blast_Score=66, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016152
- InterPro:   IPR002178
- InterPro:   IPR006320 [H]

Pfam domain/function: PF00359 PTS_EIIA_2 [H]

EC number: 2.7.1.69

Molecular weight: Translated: 18001; Mature: 18001

Theoretical pI: Translated: 5.65; Mature: 5.65

Prosite motif: PS00372 PTS_EIIA_TYPE_2_HIS ; PS51094 PTS_EIIA_TYPE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDEMTNDPALQLSSVLNIESTKSSVHCSSKKRALEIISELAAKQLNLPSQVVFDAVLTR
CCCCCCCCHHHHHHHHHCCHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
ERMGSTGIGSGIAIPHGKLEEDTLRAVGVFIRLEQPIAFDAIDNQPVDLLFALLVPADQC
HHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHEECCCEEECCCCCCHHHHHHHHHCCHHHH
KTHLHTLSLVAKRLADKTVCRRLRSAQSDDELYQIITELPPEIA
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MKDEMTNDPALQLSSVLNIESTKSSVHCSSKKRALEIISELAAKQLNLPSQVVFDAVLTR
CCCCCCCCHHHHHHHHHCCHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
ERMGSTGIGSGIAIPHGKLEEDTLRAVGVFIRLEQPIAFDAIDNQPVDLLFALLVPADQC
HHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHEECCCEEECCCCCCHHHHHHHHHCCHHHH
KTHLHTLSLVAKRLADKTVCRRLRSAQSDDELYQIITELPPEIA
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: protein N(pi)-phosphohistidine; sugar

Specific reaction: protein N(pi)-phosphohistidine + sugar = protein histidine + sugar phosphate

General reaction: Phospho group transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2695747 [H]