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The map label for this gene is trpA

Identifier: 162420964

GI number: 162420964

Start: 2433505

End: 2434320

Strand: Direct

Name: trpA

Synonym: YpAngola_A2317

Alternate gene names: 162420964

Gene position: 2433505-2434320 (Clockwise)

Preceding gene: 162420718

Following gene: 162420415

Centisome position: 54.03

GC content: 50.74

Gene sequence:

>816_bases
ATGGAGCGTTATCAGCAACTTTTTAAGCAGTTAGCCGCCAAAAAAGAAGGTGCCTTTGTTCCTTTCGTCCAGCTTGGCGA
TCCCTCTCCGGCGATGTCACTCAATATTATTGATACGTTAATTGCGGCAGGTGCAGATGCATTAGAGTTAGGGATTCCGT
TCTCTGATCCGTTGGCTGATGGCCCTACCATACAAAATGCGGCGCTGCGTGCCTTTGCTGCGGGTGTCACTCCGGGTATT
TGTTTTGAAATACTGGCCGAAATCCGCCAAAAACACCCCACAATACCTATTGGCCTGTTGATGTATGCAAATCTGGTATT
CCACAATGGTATTGATCACTTCTACCAGCGCTGTGCTGAGGTGGGTGTCGATTCGGTGCTAATTGCTGATGTGCCATTTG
AAGAGTCTGCCCCTTTCCGGGCAGCGGCCTTACGTCACGGGATTGCACCCATTTTCATCTGCCCGCCTAATGCCGACGAT
GACTTACTGCGAGAAATCGCTTCTCATGGTCGTGGTTATACTTACCTGTTATCACGTGCCGGAGTCACCGGTGCAGAAAA
TCACGGCCAGTTACCGTTAAACCATCTGGTAGATAAACTGCGTGAATATAATGCAGCGCCCGCATTGCAGGGCTTTGGTA
TTTCAGAACCCGCGCAAGTAAAAGCCAGCCTGGCGGCGGGCGCGGCGGGCGCGGCGGGTGCTATTTCAGGCTCAGCAATC
GTCAAAATCATTGAAAAAAATGTTGCACAACCCGTTGAAATGTTAGTTCAGCTAACCCGCTTCGTCACAGAGATGAAAGC
GGCAACCCGCAGCTAG

Upstream 100 bases:

>100_bases
ATCCGGATAAAGAACAAATACTGGTGGTGAATCTCTCCGGCCGGGGTGACAAAGATATTTTCACCGTTCACGATATTCTG
AAAGCACGGGGAGAGATTTA

Downstream 100 bases:

>100_bases
TTATTATTTACAATTGGTTACTATTGAGAGTGAGTTACCGTTTAGAATTAATTACTATTTACCGTTAACTGCTGTCATTA
CCGCCCTTCAGCGCCTGTTT

Product: tryptophan synthase subunit alpha

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 271; Mature: 271

Protein sequence:

>271_residues
MERYQQLFKQLAAKKEGAFVPFVQLGDPSPAMSLNIIDTLIAAGADALELGIPFSDPLADGPTIQNAALRAFAAGVTPGI
CFEILAEIRQKHPTIPIGLLMYANLVFHNGIDHFYQRCAEVGVDSVLIADVPFEESAPFRAAALRHGIAPIFICPPNADD
DLLREIASHGRGYTYLLSRAGVTGAENHGQLPLNHLVDKLREYNAAPALQGFGISEPAQVKASLAAGAAGAAGAISGSAI
VKIIEKNVAQPVEMLVQLTRFVTEMKAATRS

Sequences:

>Translated_271_residues
MERYQQLFKQLAAKKEGAFVPFVQLGDPSPAMSLNIIDTLIAAGADALELGIPFSDPLADGPTIQNAALRAFAAGVTPGI
CFEILAEIRQKHPTIPIGLLMYANLVFHNGIDHFYQRCAEVGVDSVLIADVPFEESAPFRAAALRHGIAPIFICPPNADD
DLLREIASHGRGYTYLLSRAGVTGAENHGQLPLNHLVDKLREYNAAPALQGFGISEPAQVKASLAAGAAGAAGAISGSAI
VKIIEKNVAQPVEMLVQLTRFVTEMKAATRS
>Mature_271_residues
MERYQQLFKQLAAKKEGAFVPFVQLGDPSPAMSLNIIDTLIAAGADALELGIPFSDPLADGPTIQNAALRAFAAGVTPGI
CFEILAEIRQKHPTIPIGLLMYANLVFHNGIDHFYQRCAEVGVDSVLIADVPFEESAPFRAAALRHGIAPIFICPPNADD
DLLREIASHGRGYTYLLSRAGVTGAENHGQLPLNHLVDKLREYNAAPALQGFGISEPAQVKASLAAGAAGAAGAISGSAI
VKIIEKNVAQPVEMLVQLTRFVTEMKAATRS

Specific function: The alpha subunit is responsible for the aldol cleavage of indoleglycerol phosphate to indole and glyceraldehyde 3- phosphate

COG id: COG0159

COG function: function code E; Tryptophan synthase alpha chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpA family

Homologues:

Organism=Escherichia coli, GI1787514, Length=271, Percent_Identity=77.859778597786, Blast_Score=407, Evalue=1e-115,
Organism=Saccharomyces cerevisiae, GI6321412, Length=243, Percent_Identity=30.8641975308642, Blast_Score=124, Evalue=1e-29,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): TRPA_YERPG (A9R995)

Other databases:

- EMBL:   CP000901
- RefSeq:   YP_001606750.1
- ProteinModelPortal:   A9R995
- SMR:   A9R995
- GeneID:   5800787
- GenomeReviews:   CP000901_GR
- KEGG:   ypg:YpAngola_A2317
- HOGENOM:   HBG391690
- OMA:   NPIYIYG
- ProtClustDB:   PRK13111
- BioCyc:   YPES349746:YPANGOLA_A2317-MONOMER
- HAMAP:   MF_00131
- InterPro:   IPR013785
- InterPro:   IPR011060
- InterPro:   IPR018204
- InterPro:   IPR002028
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00262

Pfam domain/function: PF00290 Trp_syntA; SSF51366 RibP_bind_barrel

EC number: =4.2.1.20

Molecular weight: Translated: 28778; Mature: 28778

Theoretical pI: Translated: 5.88; Mature: 5.88

Prosite motif: PS00167 TRP_SYNTHASE_ALPHA

Important sites: ACT_SITE 49-49 ACT_SITE 60-60

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MERYQQLFKQLAAKKEGAFVPFVQLGDPSPAMSLNIIDTLIAAGADALELGIPFSDPLAD
CHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHCCCCCEEECCCCCCCCCC
GPTIQNAALRAFAAGVTPGICFEILAEIRQKHPTIPIGLLMYANLVFHNGIDHFYQRCAE
CCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCHHHHHHHHHH
VGVDSVLIADVPFEESAPFRAAALRHGIAPIFICPPNADDDLLREIASHGRGYTYLLSRA
CCCCEEEEECCCCCCCCCHHHHHHHHCCCEEEEECCCCCHHHHHHHHHCCCCEEEEHHHC
GVTGAENHGQLPLNHLVDKLREYNAAPALQGFGISEPAQVKASLAAGAAGAAGAISGSAI
CCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCHHCCHHHH
VKIIEKNVAQPVEMLVQLTRFVTEMKAATRS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MERYQQLFKQLAAKKEGAFVPFVQLGDPSPAMSLNIIDTLIAAGADALELGIPFSDPLAD
CHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHCCCCCEEECCCCCCCCCC
GPTIQNAALRAFAAGVTPGICFEILAEIRQKHPTIPIGLLMYANLVFHNGIDHFYQRCAE
CCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCHHHHHHHHHH
VGVDSVLIADVPFEESAPFRAAALRHGIAPIFICPPNADDDLLREIASHGRGYTYLLSRA
CCCCEEEEECCCCCCCCCHHHHHHHHCCCEEEEECCCCCHHHHHHHHHCCCCEEEEHHHC
GVTGAENHGQLPLNHLVDKLREYNAAPALQGFGISEPAQVKASLAAGAAGAAGAISGSAI
CCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCHHCCHHHH
VKIIEKNVAQPVEMLVQLTRFVTEMKAATRS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA