Click here to switch to the map view.

The map label for this gene is ligB [H]

Identifier: 162420613

GI number: 162420613

Start: 53548

End: 55224

Strand: Direct

Name: ligB [H]

Synonym: YpAngola_A0046

Alternate gene names: 162420613

Gene position: 53548-55224 (Clockwise)

Preceding gene: 162418297

Following gene: 162421592

Centisome position: 1.19

GC content: 48.6

Gene sequence:

>1677_bases
ATGTTCCTATTAATATCTAACACCATAGTGGTGGGCGGTGCTTGGGCTACATCAACATGCCCTGATTGGCCTGCAACCAG
AATAGCGGTTGAGATTAACGCGTTGGAGCAACAGTTGAATAAGTGGTCGGCCGCTTACCATCAGCAAGGCCATAGCCCGG
TCACGGATGACATTTATGATCAATTACAGGACAAATTACGTGTGTGGCAATCCTGTAGGGGGTTACCTGATAAGACGGAA
AGCCAGCCGATCCCCGGCAAAGGGCAATTTCTTCACCCAGTTGCCCATACCGGGTTAAAAAAACTCAAAGATGAAACCGC
CCTCACAAGGTGGATGGCGGGGCGGAAAAATCTCTGGGTACAACCCAAGGTCGACGGTGTGGCAGTGACACTGGTTTATC
ACGGGGGGAAATTGGTGCAACTTCTTAGTCGAGGAAATGGAGTAAAAGGTCAAAACTGGACAGAAAAAGCGCCATTTATT
TCTGCTATTCCTCAATATATTGCAAATGCGCCTGCACTATTAACGTTGCAAGGGGAGTTGTTTCTACTGATGGATGGCCA
CCAGCAAGCAAAATCGGGTGGGGTGAATGCCAGATCAACGGTGGCAGGTGCACTGATGCGCAAATCTCCGTCTCCGCTAT
TAGCTCAAGTTGGGGTCTTTATTTGGGCATGGCCTGATGGCCCAACAACCATGAAAGAGAAGGTGGCACTATTACAGGTA
ATGGGTTTTCCTTTTACAGCTAAATACAGCGAGCCCGTCATGTCGCATCTTGATGTTGTGCAATGGCGTCAGTTCTGGTT
TCAAGCACCACTGCCGTTTGTCACCGATGGTGTTGTGGTCAGGCAAGAGGAGGAGCCAGCTGGTCGTTATTGGCAGGCAA
CACCGGGGCAGTGGTCTATGGCGTGGAAGTATCCGCCTTTGCAACACATTGCCGAAGTAAAAGATATTCATTTTACCCTT
GGACGAACGGGTAAAGGCACGGTGGTATTAGAGGTTTTACCGATAAAAATTGATGATAAATGGATTCGTCGAGTGAATAT
TGGTTCGGTCACCCGTTGGAAACAATGGGACATCGCTCCTGGTGATCACATTACATTGGCACTTGCCGGGCATGGTATCC
CTCGGCTAGATAACGTTGTCTGGCGAGTACACCAACGGAATACTATTACGGCACCCAATTGGGATAAATTCCATCAGCTC
AGTTGCTTTCAACGCTTACCACATGGCTGTGAACCTCAGTTTTTATCCCGCTTGATATGGCTTAGTGGCCCAGGGGGGCT
AGATATTGGCGGCATCGGTGGCGGCTTTTGGCAAGAGCTTATTCACCATGAGTTAATTAATGATTTGGTCGGATGGTTAT
TACTAACACCAGAGCAAATTGCAAGTATCCCTGGAATTGGAAATGCGCGGGCTGAAAAAATTTATCAACAGTTTCAACGG
GCAAAGCAGCAACCCTTTTCTCGTTGGTTGCTGGCATTGGGGTTCCCGCAGGTTGTTTCCGTTGACGCGCAGTGGCAGGT
TGTATTGCGCCGGAGTCTATCTGAATGGGCCACCATGGCCGGGATTGGGCAAATGAGAGCAAAACAAATTAAGCATTTTT
GGGATCACCCCGACGTACAAGCACTGGCTGATTTCTTATCTACTCAGAAAGTTGTTGGGTTCGAGCTCACAGAGTAA

Upstream 100 bases:

>100_bases
GCATTTTACTCTTTTAGGCTTCGACGGGTAGCTTATTTCGCGTTTTACTTCCGATCACAACGGAAGGAGGCTTATGAACA
TACTGAATCTAAAGATAATA

Downstream 100 bases:

>100_bases
ATCCTCCTAGGGCTATAACTTAATGGTCTGAGTGCTCATATTTGAATACAGGCAGGCCAAACCGGAATCGTAAGGCGAGT
AATCTAGCGCTAAGCCGGTG

Product: NAD-dependent DNA ligase LigB

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [NAD+] B [H]

Number of amino acids: Translated: 558; Mature: 558

Protein sequence:

>558_residues
MFLLISNTIVVGGAWATSTCPDWPATRIAVEINALEQQLNKWSAAYHQQGHSPVTDDIYDQLQDKLRVWQSCRGLPDKTE
SQPIPGKGQFLHPVAHTGLKKLKDETALTRWMAGRKNLWVQPKVDGVAVTLVYHGGKLVQLLSRGNGVKGQNWTEKAPFI
SAIPQYIANAPALLTLQGELFLLMDGHQQAKSGGVNARSTVAGALMRKSPSPLLAQVGVFIWAWPDGPTTMKEKVALLQV
MGFPFTAKYSEPVMSHLDVVQWRQFWFQAPLPFVTDGVVVRQEEEPAGRYWQATPGQWSMAWKYPPLQHIAEVKDIHFTL
GRTGKGTVVLEVLPIKIDDKWIRRVNIGSVTRWKQWDIAPGDHITLALAGHGIPRLDNVVWRVHQRNTITAPNWDKFHQL
SCFQRLPHGCEPQFLSRLIWLSGPGGLDIGGIGGGFWQELIHHELINDLVGWLLLTPEQIASIPGIGNARAEKIYQQFQR
AKQQPFSRWLLALGFPQVVSVDAQWQVVLRRSLSEWATMAGIGQMRAKQIKHFWDHPDVQALADFLSTQKVVGFELTE

Sequences:

>Translated_558_residues
MFLLISNTIVVGGAWATSTCPDWPATRIAVEINALEQQLNKWSAAYHQQGHSPVTDDIYDQLQDKLRVWQSCRGLPDKTE
SQPIPGKGQFLHPVAHTGLKKLKDETALTRWMAGRKNLWVQPKVDGVAVTLVYHGGKLVQLLSRGNGVKGQNWTEKAPFI
SAIPQYIANAPALLTLQGELFLLMDGHQQAKSGGVNARSTVAGALMRKSPSPLLAQVGVFIWAWPDGPTTMKEKVALLQV
MGFPFTAKYSEPVMSHLDVVQWRQFWFQAPLPFVTDGVVVRQEEEPAGRYWQATPGQWSMAWKYPPLQHIAEVKDIHFTL
GRTGKGTVVLEVLPIKIDDKWIRRVNIGSVTRWKQWDIAPGDHITLALAGHGIPRLDNVVWRVHQRNTITAPNWDKFHQL
SCFQRLPHGCEPQFLSRLIWLSGPGGLDIGGIGGGFWQELIHHELINDLVGWLLLTPEQIASIPGIGNARAEKIYQQFQR
AKQQPFSRWLLALGFPQVVSVDAQWQVVLRRSLSEWATMAGIGQMRAKQIKHFWDHPDVQALADFLSTQKVVGFELTE
>Mature_558_residues
MFLLISNTIVVGGAWATSTCPDWPATRIAVEINALEQQLNKWSAAYHQQGHSPVTDDIYDQLQDKLRVWQSCRGLPDKTE
SQPIPGKGQFLHPVAHTGLKKLKDETALTRWMAGRKNLWVQPKVDGVAVTLVYHGGKLVQLLSRGNGVKGQNWTEKAPFI
SAIPQYIANAPALLTLQGELFLLMDGHQQAKSGGVNARSTVAGALMRKSPSPLLAQVGVFIWAWPDGPTTMKEKVALLQV
MGFPFTAKYSEPVMSHLDVVQWRQFWFQAPLPFVTDGVVVRQEEEPAGRYWQATPGQWSMAWKYPPLQHIAEVKDIHFTL
GRTGKGTVVLEVLPIKIDDKWIRRVNIGSVTRWKQWDIAPGDHITLALAGHGIPRLDNVVWRVHQRNTITAPNWDKFHQL
SCFQRLPHGCEPQFLSRLIWLSGPGGLDIGGIGGGFWQELIHHELINDLVGWLLLTPEQIASIPGIGNARAEKIYQQFQR
AKQQPFSRWLLALGFPQVVSVDAQWQVVLRRSLSEWATMAGIGQMRAKQIKHFWDHPDVQALADFLSTQKVVGFELTE

Specific function: Catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction [H]

COG id: COG0272

COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAD-dependent DNA ligase family. LigB subfamily [H]

Homologues:

Organism=Escherichia coli, GI87082305, Length=560, Percent_Identity=43.9285714285714, Blast_Score=490, Evalue=1e-139,
Organism=Escherichia coli, GI1788750, Length=567, Percent_Identity=22.9276895943563, Blast_Score=130, Evalue=2e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020923
- InterPro:   IPR004150
- InterPro:   IPR013839
- InterPro:   IPR013840
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR010994 [H]

Pfam domain/function: PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB [H]

EC number: =6.5.1.2 [H]

Molecular weight: Translated: 62632; Mature: 62632

Theoretical pI: Translated: 9.69; Mature: 9.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFLLISNTIVVGGAWATSTCPDWPATRIAVEINALEQQLNKWSAAYHQQGHSPVTDDIYD
CEEEEECEEEEECCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHCCCCCCCHHHHH
QLQDKLRVWQSCRGLPDKTESQPIPGKGQFLHPVAHTGLKKLKDETALTRWMAGRKNLWV
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCEECHHHHHHHHHHHHHHHHHHHHCCCCCEEE
QPKVDGVAVTLVYHGGKLVQLLSRGNGVKGQNWTEKAPFISAIPQYIANAPALLTLQGEL
CCCCCCEEEEEEECCCHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHCCCCEEEEECCCE
FLLMDGHQQAKSGGVNARSTVAGALMRKSPSPLLAQVGVFIWAWPDGPTTMKEKVALLQV
EEEECCCHHHHCCCCCHHHHHHHHHHHCCCCHHHHCCCEEEEECCCCCCHHHHHHHHHHH
MGFPFTAKYSEPVMSHLDVVQWRQFWFQAPLPFVTDGVVVRQEEEPAGRYWQATPGQWSM
HCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCEECCCCCEEE
AWKYPPLQHIAEVKDIHFTLGRTGKGTVVLEVLPIKIDDKWIRRVNIGSVTRWKQWDIAP
EECCCCHHHHHHHHHHEEEECCCCCCEEEEEEEEEEECHHHHHEECCCCCCCCCCCCCCC
GDHITLALAGHGIPRLDNVVWRVHQRNTITAPNWDKFHQLSCFQRLPHGCEPQFLSRLIW
CCEEEEEEECCCCCHHHHHHHEEECCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHE
LSGPGGLDIGGIGGGFWQELIHHELINDLVGWLLLTPEQIASIPGIGNARAEKIYQQFQR
ECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCHHHHHHHHHHHH
AKQQPFSRWLLALGFPQVVSVDAQWQVVLRRSLSEWATMAGIGQMRAKQIKHFWDHPDVQ
HHHCHHHHHHHHHCCHHHEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
ALADFLSTQKVVGFELTE
HHHHHHHCCCCCEEEECC
>Mature Secondary Structure
MFLLISNTIVVGGAWATSTCPDWPATRIAVEINALEQQLNKWSAAYHQQGHSPVTDDIYD
CEEEEECEEEEECCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHCCCCCCCHHHHH
QLQDKLRVWQSCRGLPDKTESQPIPGKGQFLHPVAHTGLKKLKDETALTRWMAGRKNLWV
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCEECHHHHHHHHHHHHHHHHHHHHCCCCCEEE
QPKVDGVAVTLVYHGGKLVQLLSRGNGVKGQNWTEKAPFISAIPQYIANAPALLTLQGEL
CCCCCCEEEEEEECCCHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHCCCCEEEEECCCE
FLLMDGHQQAKSGGVNARSTVAGALMRKSPSPLLAQVGVFIWAWPDGPTTMKEKVALLQV
EEEECCCHHHHCCCCCHHHHHHHHHHHCCCCHHHHCCCEEEEECCCCCCHHHHHHHHHHH
MGFPFTAKYSEPVMSHLDVVQWRQFWFQAPLPFVTDGVVVRQEEEPAGRYWQATPGQWSM
HCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCEECCCCCEEE
AWKYPPLQHIAEVKDIHFTLGRTGKGTVVLEVLPIKIDDKWIRRVNIGSVTRWKQWDIAP
EECCCCHHHHHHHHHHEEEECCCCCCEEEEEEEEEEECHHHHHEECCCCCCCCCCCCCCC
GDHITLALAGHGIPRLDNVVWRVHQRNTITAPNWDKFHQLSCFQRLPHGCEPQFLSRLIW
CCEEEEEEECCCCCHHHHHHHEEECCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHE
LSGPGGLDIGGIGGGFWQELIHHELINDLVGWLLLTPEQIASIPGIGNARAEKIYQQFQR
ECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCHHHHHHHHHHHH
AKQQPFSRWLLALGFPQVVSVDAQWQVVLRRSLSEWATMAGIGQMRAKQIKHFWDHPDVQ
HHHCHHHHHHHHHCCHHHEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
ALADFLSTQKVVGFELTE
HHHHHHHCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA