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The map label for this gene is bcr [H]

Identifier: 162418818

GI number: 162418818

Start: 1541338

End: 1542537

Strand: Direct

Name: bcr [H]

Synonym: YpAngola_A1491

Alternate gene names: 162418818

Gene position: 1541338-1542537 (Clockwise)

Preceding gene: 162418753

Following gene: 162421333

Centisome position: 34.22

GC content: 50.17

Gene sequence:

>1200_bases
GTGCAAAAAAACCGGACATCCCACATTGGTTTGATTTTTATCCTTGGGTTGTTGTCGATGCTGATGCCTTTGGCGATTGA
TATGTATCTGCCAAGCATGCCGATTATTGCGCAGGAGTTTGGGGTCGAGAGTGGCCGTGTGCAAATGACGCTGAGCGCAT
ATGTACTGGGTTTTGCCATTGGGCAAATGTTCTATGGGCCAATGGCTGACAGCATCGGGCGTAAGCCGGTCATTTTTTGG
GGGACGCTGATTTTTGCGCTTGCAGGTATGGCATGTGCGTTGGTGCAGTCGGTCGAGCAACTCATTAATATGCGCTTCCT
GCATGGCTTATCCGCCGCCGCCGCAAGTGTGGTGATTAATGCCTTGATGCGCGATATGTTCACCAAAGATGAGTTCTCCC
GAATGATGTCTTTCGTGGTATTGGTGATGACGATTGCCCCCTTGCTCGCACCCATTATCGGTGGTGCACTGTTGGTGTGG
TTTAGCTGGCATGCTATTTTCTGGACCATGGCGGGGGCGGCACTGGTTGGTGCGGTGTTAGTGGCGCTGTTTATCCAAGA
AACATTGCCTCCTGAACGGCGACAGAAATTTCATCTGCGCACGACCATAGGTAATTTTGGCTCGTTATTTCGCCACAAAC
GCGTACTGAGCTATATGCTGGCCAGTGGTTTCTCTTTTGCCGGTATGTTCTCATTCCTCAGCGCCGGTCCTTTTGTTTAT
ATCGATTTAAACGGTGTTTCCCCGCAAAACTTTGGTTATTACTTTGCACTGAACATTGTCTTTTTGTTCTTGATGACCTT
TATCAACAGCCGTAATGTGCGCCGTTTCGGGGCGCTGAAAATGTTCCGTTTTGGCCTGATAGTGCAATTTGTGATGGGGA
TCTGGCTGGTAGTGGTTTGTGTCGCTGGCCTAGGATTCTGGGCCTTAGTTCTGGGCGTAGCTGCCTATGTTGGGTGTATC
GCAATGATCACGTCTAACGCGATGGCAGTGATATTAGATGATTTCCCGCATATGGCAGGGACCGCCTCTTCTTTGGCGGG
GACGTTGCGCTTTGGTATTGGTGCGGTGGTGGGGACGTTACTGCCGATGGCTTCGTCGCGCAGCGCCTGGCCAATGGTCA
GTTCAATGGCATTTTGTATTATTGTCGCGATGTTGTTGTATCTGTATGCCAGTCGCCCAAGTCATAAAACCGTGTCATAA

Upstream 100 bases:

>100_bases
GATTGTTTGTAATGAGATTGTTATTGCCGTGCGGGCCAGCGGTTCCGCACCGTTCTACTGCGGTATAATCTGCGGTAAGT
TTTTGGAGGAGTGGTAAAGC

Downstream 100 bases:

>100_bases
AAAATAGCCAGCGCCCATGCCGGATGGTATTCGATATGGATGGTATTCGACATGGATGGTATTTGACATGGATTGTGAAC
GGTATGAGTGGTCGGCCATT

Product: bicyclomycin/multidrug efflux system

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: Sulfonamide resistance protein [H]

Number of amino acids: Translated: 399; Mature: 399

Protein sequence:

>399_residues
MQKNRTSHIGLIFILGLLSMLMPLAIDMYLPSMPIIAQEFGVESGRVQMTLSAYVLGFAIGQMFYGPMADSIGRKPVIFW
GTLIFALAGMACALVQSVEQLINMRFLHGLSAAAASVVINALMRDMFTKDEFSRMMSFVVLVMTIAPLLAPIIGGALLVW
FSWHAIFWTMAGAALVGAVLVALFIQETLPPERRQKFHLRTTIGNFGSLFRHKRVLSYMLASGFSFAGMFSFLSAGPFVY
IDLNGVSPQNFGYYFALNIVFLFLMTFINSRNVRRFGALKMFRFGLIVQFVMGIWLVVVCVAGLGFWALVLGVAAYVGCI
AMITSNAMAVILDDFPHMAGTASSLAGTLRFGIGAVVGTLLPMASSRSAWPMVSSMAFCIIVAMLLYLYASRPSHKTVS

Sequences:

>Translated_399_residues
MQKNRTSHIGLIFILGLLSMLMPLAIDMYLPSMPIIAQEFGVESGRVQMTLSAYVLGFAIGQMFYGPMADSIGRKPVIFW
GTLIFALAGMACALVQSVEQLINMRFLHGLSAAAASVVINALMRDMFTKDEFSRMMSFVVLVMTIAPLLAPIIGGALLVW
FSWHAIFWTMAGAALVGAVLVALFIQETLPPERRQKFHLRTTIGNFGSLFRHKRVLSYMLASGFSFAGMFSFLSAGPFVY
IDLNGVSPQNFGYYFALNIVFLFLMTFINSRNVRRFGALKMFRFGLIVQFVMGIWLVVVCVAGLGFWALVLGVAAYVGCI
AMITSNAMAVILDDFPHMAGTASSLAGTLRFGIGAVVGTLLPMASSRSAWPMVSSMAFCIIVAMLLYLYASRPSHKTVS
>Mature_399_residues
MQKNRTSHIGLIFILGLLSMLMPLAIDMYLPSMPIIAQEFGVESGRVQMTLSAYVLGFAIGQMFYGPMADSIGRKPVIFW
GTLIFALAGMACALVQSVEQLINMRFLHGLSAAAASVVINALMRDMFTKDEFSRMMSFVVLVMTIAPLLAPIIGGALLVW
FSWHAIFWTMAGAALVGAVLVALFIQETLPPERRQKFHLRTTIGNFGSLFRHKRVLSYMLASGFSFAGMFSFLSAGPFVY
IDLNGVSPQNFGYYFALNIVFLFLMTFINSRNVRRFGALKMFRFGLIVQFVMGIWLVVVCVAGLGFWALVLGVAAYVGCI
AMITSNAMAVILDDFPHMAGTASSLAGTLRFGIGAVVGTLLPMASSRSAWPMVSSMAFCIIVAMLLYLYASRPSHKTVS

Specific function: Involved in sulfonamide (sulfathiazole) and bicyclomycin resistance. Probable membrane translocase [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Bcr/CmlA family [H]

Homologues:

Organism=Escherichia coli, GI1788509, Length=396, Percent_Identity=68.4343434343434, Blast_Score=549, Evalue=1e-157,
Organism=Escherichia coli, GI48994889, Length=357, Percent_Identity=29.6918767507003, Blast_Score=126, Evalue=2e-30,
Organism=Escherichia coli, GI1790146, Length=241, Percent_Identity=28.6307053941909, Blast_Score=112, Evalue=3e-26,
Organism=Escherichia coli, GI87082312, Length=389, Percent_Identity=25.9640102827763, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI1787065, Length=257, Percent_Identity=25.6809338521401, Blast_Score=85, Evalue=9e-18,
Organism=Escherichia coli, GI1790794, Length=230, Percent_Identity=24.3478260869565, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1790195, Length=166, Percent_Identity=26.5060240963855, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI87081738, Length=366, Percent_Identity=26.775956284153, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1787880, Length=203, Percent_Identity=26.6009852216749, Blast_Score=63, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6319480, Length=199, Percent_Identity=25.1256281407035, Blast_Score=70, Evalue=6e-13,
Organism=Saccharomyces cerevisiae, GI6322071, Length=180, Percent_Identity=25.5555555555556, Blast_Score=66, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6319517, Length=187, Percent_Identity=23.5294117647059, Blast_Score=64, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6324264, Length=159, Percent_Identity=24.5283018867925, Blast_Score=63, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004812
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR004734 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 43638; Mature: 43638

Theoretical pI: Translated: 10.20; Mature: 10.20

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
7.0 %Met     (Translated Protein)
8.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
7.0 %Met     (Mature Protein)
8.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure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HHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8486276; 9278503; 2694948 [H]