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The map label for this gene is gltD [H]

Identifier: 162227937

GI number: 162227937

Start: 453161

End: 454627

Strand: Direct

Name: gltD [H]

Synonym: Bcen2424_0403

Alternate gene names: 162227937

Gene position: 453161-454627 (Clockwise)

Preceding gene: 116688426

Following gene: 116688428

Centisome position: 13.01

GC content: 66.19

Gene sequence:

>1467_bases
ATGGGCAAGGCAACCGGTTTTCTGGAGTTCGAACGCCGCCACGAGGCGTACGAAGCACCGCTCACGCGCGTGAAGCACTA
CAAGGAATTCGTCGCGGCACTGACCGACGCGGACGCGAAGATCCAGGGCGCGCGCTGCATGGATTGCGGCATCCCGTTCT
GCAACAACGGCTGCCCGGTCAACAACATCATCCCGGACTTCAACGATCTCGTTTACCGCCAGGACTGGCAGCAGGCGATC
GAAGTCCTGCACTCGACCAACAACTTCCCGGAATTCACGGGCCGCATCTGCCCGGCGCCGTGCGAAGCGGCGTGCACGCT
CGGGATCAACGACGACCCGGTCGGCATCAAGTCGATCGAGCACGCGATCATCGACAAGGCTTGGGCGGAAGGTTGGGTCA
AGCCGCTGCCGGCCGAACACAAGACGGGCAAGAAGGTCGCGGTCGTCGGTTCGGGCCCCGCGGGCCTCGCCGCCGCGCAG
CAGCTCGCGCGGGCGGGCCACGACGTGACGGTGTTCGAGAAGAACGACCGCATCGGCGGCCTGCTGCGTTACGGGATTCC
CGACTTCAAGCTCGAGAAGTGGCTGATCGACCGCCGCATGCGCCAGATGGAAGCGGAAGGCGTGACGTTCCGCACCAGCG
TGTTCATCGGCAAGGACCCGCTGCCGGAGTCGATCGGCAGCCTCGCGAAGGAAACCATTTCGCCGGACACGCTGAAGGAA
GAATTCGACGCGGTCGTGATCGCCGGCGGTTCGGAAACGCCGCGCGACCTGCCGGTGCCGGGCCGCGAGCTCGCGGGCGT
CCATTTCGCGATGGACTTCCTGCCGCAGCAGAACCGCGTGAACGCGGGCGACAAGCTGGCTGACCAGCTGCTCGCGAAGG
GCAAGCACGTGATCGTGATCGGCGGCGGCGATACGGGTTCGGACTGCGTCGGCACGTCGAACCGTCACGGCGCGAAGCAC
GTCACGCAGTTCGAGCTGCTGCCGCAGCCGCCGGAAGAGGAAAACAAGCCGCTCGTGTGGCCGTACTGGCCGATCAAGCT
GCGCACGTCGTCGTCGCACGACGAAGGCTGCGAGCGTGACTGGGCGGTCGCGACGAAGCGTCTCGAAGGCAAGAACGGCA
AGGTCGAGAAGCTGATCGCGGTGCGCGTCGAGTGGAAGGACGGCAAGATGCAGGAAGTGCCGGGTTCCGAGTTCGAGATG
AAGGCCGATCTCGTGCTGCTCGCGATGGGCTTCACGCAGCCGGCCGCGCCGGTGCTCGACGCGTTCGGCGTCGCGAAGGA
TGCGCGCGGCAATGCGCGTGCGGCAACCGAAGGCGATCGTTCGTACTACACGTCGGTCGACAAGGTGTTCGCGGCAGGCG
ACATGCGTCGCGGCCAGTCGCTCGTGGTGTGGGCGATCCGCGAAGGCCGCCAGTGCGCGCGCTCGGTCGACGCGTACCTG
ATGGGGCATTCGGAACTGCCGCGCTGA

Upstream 100 bases:

>100_bases
GTGCTGGCCGCCTGAGCGACGAACGACATAGCGAATGCCGCGCGCGCCTGACGGCCGCGCGCGGCTCCCCCACCCGATAC
ACCGAACAGAAGAGCACCTT

Downstream 100 bases:

>100_bases
GTTGAAGCGCGCGAGCGGGTTTCGATGGAAGCCGGGCGTCCGAAAGGGCGCCCGGTTTTTTTATGGCTGCGCGCGCGATC
GATGTCGTTGCATCGGCGTC

Product: glutamate synthase subunit beta

Products: NA

Alternate protein names: NADPH-GOGAT [H]

Number of amino acids: Translated: 488; Mature: 487

Protein sequence:

>488_residues
MGKATGFLEFERRHEAYEAPLTRVKHYKEFVAALTDADAKIQGARCMDCGIPFCNNGCPVNNIIPDFNDLVYRQDWQQAI
EVLHSTNNFPEFTGRICPAPCEAACTLGINDDPVGIKSIEHAIIDKAWAEGWVKPLPAEHKTGKKVAVVGSGPAGLAAAQ
QLARAGHDVTVFEKNDRIGGLLRYGIPDFKLEKWLIDRRMRQMEAEGVTFRTSVFIGKDPLPESIGSLAKETISPDTLKE
EFDAVVIAGGSETPRDLPVPGRELAGVHFAMDFLPQQNRVNAGDKLADQLLAKGKHVIVIGGGDTGSDCVGTSNRHGAKH
VTQFELLPQPPEEENKPLVWPYWPIKLRTSSSHDEGCERDWAVATKRLEGKNGKVEKLIAVRVEWKDGKMQEVPGSEFEM
KADLVLLAMGFTQPAAPVLDAFGVAKDARGNARAATEGDRSYYTSVDKVFAAGDMRRGQSLVVWAIREGRQCARSVDAYL
MGHSELPR

Sequences:

>Translated_488_residues
MGKATGFLEFERRHEAYEAPLTRVKHYKEFVAALTDADAKIQGARCMDCGIPFCNNGCPVNNIIPDFNDLVYRQDWQQAI
EVLHSTNNFPEFTGRICPAPCEAACTLGINDDPVGIKSIEHAIIDKAWAEGWVKPLPAEHKTGKKVAVVGSGPAGLAAAQ
QLARAGHDVTVFEKNDRIGGLLRYGIPDFKLEKWLIDRRMRQMEAEGVTFRTSVFIGKDPLPESIGSLAKETISPDTLKE
EFDAVVIAGGSETPRDLPVPGRELAGVHFAMDFLPQQNRVNAGDKLADQLLAKGKHVIVIGGGDTGSDCVGTSNRHGAKH
VTQFELLPQPPEEENKPLVWPYWPIKLRTSSSHDEGCERDWAVATKRLEGKNGKVEKLIAVRVEWKDGKMQEVPGSEFEM
KADLVLLAMGFTQPAAPVLDAFGVAKDARGNARAATEGDRSYYTSVDKVFAAGDMRRGQSLVVWAIREGRQCARSVDAYL
MGHSELPR
>Mature_487_residues
GKATGFLEFERRHEAYEAPLTRVKHYKEFVAALTDADAKIQGARCMDCGIPFCNNGCPVNNIIPDFNDLVYRQDWQQAIE
VLHSTNNFPEFTGRICPAPCEAACTLGINDDPVGIKSIEHAIIDKAWAEGWVKPLPAEHKTGKKVAVVGSGPAGLAAAQQ
LARAGHDVTVFEKNDRIGGLLRYGIPDFKLEKWLIDRRMRQMEAEGVTFRTSVFIGKDPLPESIGSLAKETISPDTLKEE
FDAVVIAGGSETPRDLPVPGRELAGVHFAMDFLPQQNRVNAGDKLADQLLAKGKHVIVIGGGDTGSDCVGTSNRHGAKHV
TQFELLPQPPEEENKPLVWPYWPIKLRTSSSHDEGCERDWAVATKRLEGKNGKVEKLIAVRVEWKDGKMQEVPGSEFEMK
ADLVLLAMGFTQPAAPVLDAFGVAKDARGNARAATEGDRSYYTSVDKVFAAGDMRRGQSLVVWAIREGRQCARSVDAYLM
GHSELPR

Specific function: NITROGEN METABOLISM, GLUTAMATE BIOSYNTHESIS. THE CATALYZED REACTION BRINGS TOGETHER THE NITROGEN AND CARBON METABOLISM. [C]

COG id: COG0493

COG function: function code ER; NADPH-dependent glutamate synthase beta chain and related oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI119943098, Length=493, Percent_Identity=26.1663286004057, Blast_Score=112, Evalue=8e-25,
Organism=Escherichia coli, GI1789606, Length=486, Percent_Identity=34.5679012345679, Blast_Score=248, Evalue=5e-67,
Organism=Escherichia coli, GI1788811, Length=455, Percent_Identity=36.4835164835165, Blast_Score=245, Evalue=5e-66,
Organism=Escherichia coli, GI87082180, Length=467, Percent_Identity=33.8329764453961, Blast_Score=218, Evalue=9e-58,
Organism=Escherichia coli, GI1788468, Length=463, Percent_Identity=29.1576673866091, Blast_Score=154, Evalue=1e-38,
Organism=Escherichia coli, GI1789243, Length=444, Percent_Identity=28.3783783783784, Blast_Score=118, Evalue=9e-28,
Organism=Caenorhabditis elegans, GI17570289, Length=506, Percent_Identity=45.8498023715415, Blast_Score=431, Evalue=1e-121,
Organism=Caenorhabditis elegans, GI71984108, Length=484, Percent_Identity=26.2396694214876, Blast_Score=100, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6320030, Length=502, Percent_Identity=50.199203187251, Blast_Score=475, Evalue=1e-135,
Organism=Drosophila melanogaster, GI28574881, Length=503, Percent_Identity=49.5029821073559, Blast_Score=474, Evalue=1e-134,
Organism=Drosophila melanogaster, GI24665539, Length=503, Percent_Identity=49.5029821073559, Blast_Score=474, Evalue=1e-134,
Organism=Drosophila melanogaster, GI24665547, Length=503, Percent_Identity=49.7017892644135, Blast_Score=473, Evalue=1e-133,
Organism=Drosophila melanogaster, GI24665543, Length=503, Percent_Identity=49.7017892644135, Blast_Score=473, Evalue=1e-133,
Organism=Drosophila melanogaster, GI24640763, Length=484, Percent_Identity=24.1735537190083, Blast_Score=96, Evalue=7e-20,
Organism=Drosophila melanogaster, GI18858217, Length=484, Percent_Identity=24.1735537190083, Blast_Score=96, Evalue=7e-20,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013027
- InterPro:   IPR012285
- InterPro:   IPR006005
- InterPro:   IPR009051
- InterPro:   IPR016040
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: =1.4.1.13 [H]

Molecular weight: Translated: 53554; Mature: 53423

Theoretical pI: Translated: 6.38; Mature: 6.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKATGFLEFERRHEAYEAPLTRVKHYKEFVAALTDADAKIQGARCMDCGIPFCNNGCPV
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCEEEECCCCCCCCCCCC
NNIIPDFNDLVYRQDWQQAIEVLHSTNNFPEFTGRICPAPCEAACTLGINDDPVGIKSIE
CCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHCCCCCCCCCCCEEEECCCCCCCCHHHHH
HAIIDKAWAEGWVKPLPAEHKTGKKVAVVGSGPAGLAAAQQLARAGHDVTVFEKNDRIGG
HHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHCCCCEEEEECCCCCCC
LLRYGIPDFKLEKWLIDRRMRQMEAEGVTFRTSVFIGKDPLPESIGSLAKETISPDTLKE
EEECCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCHHHHHHHHHHHCCHHHHHH
EFDAVVIAGGSETPRDLPVPGRELAGVHFAMDFLPQQNRVNAGDKLADQLLAKGKHVIVI
HCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCEEEEE
GGGDTGSDCVGTSNRHGAKHVTQFELLPQPPEEENKPLVWPYWPIKLRTSSSHDEGCERD
ECCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCEEECCCCEEEECCCCCCCCCCHH
WAVATKRLEGKNGKVEKLIAVRVEWKDGKMQEVPGSEFEMKADLVLLAMGFTQPAAPVLD
HHHHHHHHCCCCCCEEEEEEEEEEECCCCCCCCCCCCCEEECCEEEEEECCCCCCHHHHH
AFGVAKDARGNARAATEGDRSYYTSVDKVFAAGDMRRGQSLVVWAIREGRQCARSVDAYL
HHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEEEEHHHHHHHHHHHHHH
MGHSELPR
CCCCCCCC
>Mature Secondary Structure 
GKATGFLEFERRHEAYEAPLTRVKHYKEFVAALTDADAKIQGARCMDCGIPFCNNGCPV
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCEEEECCCCCCCCCCCC
NNIIPDFNDLVYRQDWQQAIEVLHSTNNFPEFTGRICPAPCEAACTLGINDDPVGIKSIE
CCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHCCCCCCCCCCCEEEECCCCCCCCHHHHH
HAIIDKAWAEGWVKPLPAEHKTGKKVAVVGSGPAGLAAAQQLARAGHDVTVFEKNDRIGG
HHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHCCCCEEEEECCCCCCC
LLRYGIPDFKLEKWLIDRRMRQMEAEGVTFRTSVFIGKDPLPESIGSLAKETISPDTLKE
EEECCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCHHHHHHHHHHHCCHHHHHH
EFDAVVIAGGSETPRDLPVPGRELAGVHFAMDFLPQQNRVNAGDKLADQLLAKGKHVIVI
HCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCEEEEE
GGGDTGSDCVGTSNRHGAKHVTQFELLPQPPEEENKPLVWPYWPIKLRTSSSHDEGCERD
ECCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCEEECCCCEEEECCCCCCCCCCHH
WAVATKRLEGKNGKVEKLIAVRVEWKDGKMQEVPGSEFEMKADLVLLAMGFTQPAAPVLD
HHHHHHHHCCCCCCEEEEEEEEEEECCCCCCCCCCCCCEEECCEEEEEECCCCCCHHHHH
AFGVAKDARGNARAATEGDRSYYTSVDKVFAAGDMRRGQSLVVWAIREGRQCARSVDAYL
HHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEEEEHHHHHHHHHHHHHH
MGHSELPR
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377; 11029411 [H]