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The map label for this gene is hslU [H]

Identifier: 162149054

GI number: 162149054

Start: 3375919

End: 3377235

Strand: Reverse

Name: hslU [H]

Synonym: GDI_3284

Alternate gene names: 162149054

Gene position: 3377235-3375919 (Counterclockwise)

Preceding gene: 162149055

Following gene: 162149053

Centisome position: 85.63

GC content: 67.73

Gene sequence:

>1317_bases
ATGGACATTCCCAACCATTCCCCCCGTGAGATCGTCTCGGAACTCGATCGCTTCATCATCGGCCAGACCGATGCCAAGCG
CGCGGTGGCTATCGCGCTGCGCAACCGCTGGCGCCGGGCGCAACTGGCCGACGGGCTGCGCGACGAGGTGGTGCCGAAGA
ACATCCTGATGATCGGCCCGACCGGCTGCGGCAAGACCGAGATCGCCCGCCGCCTGGCCCGTCTGGCGCAGGCGCCGTTC
CTGAAGGTCGAGGCCACCAAGTTCACCGAGGTCGGCTATGTCGGCCGCGATGTCGAAAGCATCGTGCGCGACCTGGTCGA
GGTGTCGCTGAACATGCTGCGCGACCTGCGCCGCCGCGACGTGCAGGCGCGCGCCGAACTGGCCGCCGAAAACCGGCTGG
TGGACGCGCTGGTGGGCGAGGGTGCCTCGGCCGACACCAAGGGCAAGTTCCGCCGCATGCTGCGCAATGGCGAGCTGGAG
GAGAAGGAGGTCGAAATCTCGATCGCCGACACCCAGGCACCGGGCAGCATGGGCGACATGGGGAACATGACGTCCGGTAC
GGTCATCAATTTCTCCGACATGATGAAGGGATTGATGAACCGCGTGCCGCAGCGCCGCCGCATGACCGTCGCCGCCGCGC
GCGAGGCCCTGACGCGCGAGGAAGCGGACAAGATGCTGGACAACGACGCCCTGACCGGCGAGGCGGTGGCCCATGCCCAG
GATCACGGCATCGTCTTCCTGGACGAGATCGACAAGGTCTGCGCCCGGTCGTCGGAAAGCGGGTTCCGGGGCGGCGACGT
CTCGCGCGAGGGCGTGCAGCGCGACCTGCTGCCGCTGATCGAGGGCACGACGGTATCCACCAAATACGGGCCGGTGAAGA
CGGACCATATCCTGTTCATCGCCTCGGGCGCGTTCCACATCGCCAAGCCGTCGGACCTGCTGCCGGAATTGCAGGGGCGC
CTGCCGATCCGTGTCGAGCTGGCGCCGCTGACGCGCGAGGACCTGCGGCGCATCCTGACCGAGCCGGAACATTCGCTGCT
GAAGCAATATACCGCGCTGCTGGGGACCGAGGGCGTGACCCTGGAGTTCTCGGACGACGCGGTGGACGCGCTGGCGGAAC
TGGCCGCCGACATCAACGAGCGGATCGAGAATATCGGCGCGCGGCGCCTGGCGACGGTGCTGGAGCGGCTGCTGGAAGAG
GTCTCGTTCACGGCGTCCGACCGTTCGGGCCAGGCGGTGCGCATCACCGCCGCCGACGTGCAGGACAAGGTGGCGCCGCT
GGCGCGCAAGGGCGACCTCAGCCGCTTCATTCTTTAA

Upstream 100 bases:

>100_bases
GGGCGATGCGCATCGCCGGCGAGATCTGCGTCTATACCAATCATTCCGTGATTCTGGAGACCCTGGGCCCGGATGAGGCT
CCGGGAGAGGCTGCCGCGTA

Downstream 100 bases:

>100_bases
ATTACGTGGATCAGATAGCCTTATGACCGAGCCGTTCGTCAGGCCCGAGGACGACACCGCCGCCGAGGCGGTTGCCGCCA
TCGGCGAGGAACTCGACCTG

Product: ATP-dependent protease ATP-binding subunit HslU

Products: NA

Alternate protein names: Unfoldase HslU [H]

Number of amino acids: Translated: 438; Mature: 438

Protein sequence:

>438_residues
MDIPNHSPREIVSELDRFIIGQTDAKRAVAIALRNRWRRAQLADGLRDEVVPKNILMIGPTGCGKTEIARRLARLAQAPF
LKVEATKFTEVGYVGRDVESIVRDLVEVSLNMLRDLRRRDVQARAELAAENRLVDALVGEGASADTKGKFRRMLRNGELE
EKEVEISIADTQAPGSMGDMGNMTSGTVINFSDMMKGLMNRVPQRRRMTVAAAREALTREEADKMLDNDALTGEAVAHAQ
DHGIVFLDEIDKVCARSSESGFRGGDVSREGVQRDLLPLIEGTTVSTKYGPVKTDHILFIASGAFHIAKPSDLLPELQGR
LPIRVELAPLTREDLRRILTEPEHSLLKQYTALLGTEGVTLEFSDDAVDALAELAADINERIENIGARRLATVLERLLEE
VSFTASDRSGQAVRITAADVQDKVAPLARKGDLSRFIL

Sequences:

>Translated_438_residues
MDIPNHSPREIVSELDRFIIGQTDAKRAVAIALRNRWRRAQLADGLRDEVVPKNILMIGPTGCGKTEIARRLARLAQAPF
LKVEATKFTEVGYVGRDVESIVRDLVEVSLNMLRDLRRRDVQARAELAAENRLVDALVGEGASADTKGKFRRMLRNGELE
EKEVEISIADTQAPGSMGDMGNMTSGTVINFSDMMKGLMNRVPQRRRMTVAAAREALTREEADKMLDNDALTGEAVAHAQ
DHGIVFLDEIDKVCARSSESGFRGGDVSREGVQRDLLPLIEGTTVSTKYGPVKTDHILFIASGAFHIAKPSDLLPELQGR
LPIRVELAPLTREDLRRILTEPEHSLLKQYTALLGTEGVTLEFSDDAVDALAELAADINERIENIGARRLATVLERLLEE
VSFTASDRSGQAVRITAADVQDKVAPLARKGDLSRFIL
>Mature_438_residues
MDIPNHSPREIVSELDRFIIGQTDAKRAVAIALRNRWRRAQLADGLRDEVVPKNILMIGPTGCGKTEIARRLARLAQAPF
LKVEATKFTEVGYVGRDVESIVRDLVEVSLNMLRDLRRRDVQARAELAAENRLVDALVGEGASADTKGKFRRMLRNGELE
EKEVEISIADTQAPGSMGDMGNMTSGTVINFSDMMKGLMNRVPQRRRMTVAAAREALTREEADKMLDNDALTGEAVAHAQ
DHGIVFLDEIDKVCARSSESGFRGGDVSREGVQRDLLPLIEGTTVSTKYGPVKTDHILFIASGAFHIAKPSDLLPELQGR
LPIRVELAPLTREDLRRILTEPEHSLLKQYTALLGTEGVTLEFSDDAVDALAELAADINERIENIGARRLATVLERLLEE
VSFTASDRSGQAVRITAADVQDKVAPLARKGDLSRFIL

Specific function: ATPase subunit of a proteasome-like degradation complex; this subunit has chaperone activity. The binding of ATP and its subsequent hydrolysis by HslU are essential for unfolding of protein substrates subsequently hydrolyzed by HslV. HslU recognizes the N

COG id: COG1220

COG function: function code O; ATP-dependent protease HslVU (ClpYQ), ATPase subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ClpX chaperone family. HslU subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790366, Length=449, Percent_Identity=54.56570155902, Blast_Score=457, Evalue=1e-130,
Organism=Escherichia coli, GI1786642, Length=102, Percent_Identity=46.078431372549, Blast_Score=95, Evalue=8e-21,
Organism=Caenorhabditis elegans, GI71982908, Length=244, Percent_Identity=32.3770491803279, Blast_Score=90, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI71982905, Length=244, Percent_Identity=32.3770491803279, Blast_Score=89, Evalue=3e-18,
Organism=Saccharomyces cerevisiae, GI6319704, Length=141, Percent_Identity=34.7517730496454, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24648289, Length=249, Percent_Identity=31.7269076305221, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24648291, Length=249, Percent_Identity=31.7269076305221, Blast_Score=97, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013093
- InterPro:   IPR003959
- InterPro:   IPR019489
- InterPro:   IPR004491 [H]

Pfam domain/function: PF00004 AAA; PF07724 AAA_2; PF10431 ClpB_D2-small [H]

EC number: NA

Molecular weight: Translated: 48265; Mature: 48265

Theoretical pI: Translated: 5.45; Mature: 5.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDIPNHSPREIVSELDRFIIGQTDAKRAVAIALRNRWRRAQLADGLRDEVVPKNILMIGP
CCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCEEEECC
TGCGKTEIARRLARLAQAPFLKVEATKFTEVGYVGRDVESIVRDLVEVSLNMLRDLRRRD
CCCCHHHHHHHHHHHHHCCCEEEECCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
VQARAELAAENRLVDALVGEGASADTKGKFRRMLRNGELEEKEVEISIADTQAPGSMGDM
HHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCCCC
GNMTSGTVINFSDMMKGLMNRVPQRRRMTVAAAREALTREEADKMLDNDALTGEAVAHAQ
CCCCCCCEEEHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHC
DHGIVFLDEIDKVCARSSESGFRGGDVSREGVQRDLLPLIEGTTVSTKYGPVKTDHILFI
CCCEEEHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEECCCCCCCCCEEEEE
ASGAFHIAKPSDLLPELQGRLPIRVELAPLTREDLRRILTEPEHSLLKQYTALLGTEGVT
ECCCEEECCCHHHHHHHHCCCCEEEEECCCCHHHHHHHHCCCHHHHHHHHHHHHCCCCCE
LEFSDDAVDALAELAADINERIENIGARRLATVLERLLEEVSFTASDRSGQAVRITAADV
EEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEHHHH
QDKVAPLARKGDLSRFIL
HHHHHHHHHCCCHHHHCC
>Mature Secondary Structure
MDIPNHSPREIVSELDRFIIGQTDAKRAVAIALRNRWRRAQLADGLRDEVVPKNILMIGP
CCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCEEEECC
TGCGKTEIARRLARLAQAPFLKVEATKFTEVGYVGRDVESIVRDLVEVSLNMLRDLRRRD
CCCCHHHHHHHHHHHHHCCCEEEECCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
VQARAELAAENRLVDALVGEGASADTKGKFRRMLRNGELEEKEVEISIADTQAPGSMGDM
HHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCCCC
GNMTSGTVINFSDMMKGLMNRVPQRRRMTVAAAREALTREEADKMLDNDALTGEAVAHAQ
CCCCCCCEEEHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHC
DHGIVFLDEIDKVCARSSESGFRGGDVSREGVQRDLLPLIEGTTVSTKYGPVKTDHILFI
CCCEEEHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEECCCCCCCCCEEEEE
ASGAFHIAKPSDLLPELQGRLPIRVELAPLTREDLRRILTEPEHSLLKQYTALLGTEGVT
ECCCEEECCCHHHHHHHHCCCCEEEEECCCCHHHHHHHHCCCHHHHHHHHHHHHCCCCCE
LEFSDDAVDALAELAADINERIENIGARRLATVLERLLEEVSFTASDRSGQAVRITAADV
EEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEHHHH
QDKVAPLARKGDLSRFIL
HHHHHHHHHCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA