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The map label for this gene is pheS

Identifier: 162149042

GI number: 162149042

Start: 3363500

End: 3364570

Strand: Reverse

Name: pheS

Synonym: GDI_3272

Alternate gene names: 162149042

Gene position: 3364570-3363500 (Counterclockwise)

Preceding gene: 162149043

Following gene: 162149041

Centisome position: 85.31

GC content: 68.91

Gene sequence:

>1071_bases
ATGAGCGACGATCTCGAAACCCTGCGGCAGGAGACGCTGGCCGCGCTGGCCGCCGCCACCGACCGGCGGGAATGGGACGC
GGTGCGTGTCGGCACGCTGGGCAAGTCCGGGCGGCTGACCGCGCTGCTGAAGGAACTGGGCCGGATGACGCCCGAGGCGC
GCAAGCTGCGCGGCGCCGCGGTCAACCGGCTGCGCGACGACCTGACGCGCGAGATCGAGGCCCGCGGGGCGGAGATCGAA
AGCGCGATCCTGAATGCGCGCCTGGCGGCCGAACGGGTGGACGTCACCCTGCCGGTCCGCCCCGAATCTTCGGGCGCGAT
CCATCCGATCAGCCGGACGATGGAGGAAATGGCCGCCATTTTCGGCGCCATGGGCTTCAAGGTGGCCGAAGGGCCGGATA
TCGAGACCGACTGGCACAATTTCTCGGCCCTGAATACGCCCGACCACCATCCGGCCCGCACCGACCAGGACACGTTCTAC
CTGCCCGCCCGGGAAGGGACGTCGCAGGAGCGCGTGCTGCGTACCCAGACCTCGGGCGTGCAGATCCGCACCATGCTGGG
CGAGGAGCCGCCGATCCGCATCATCGCGCCCGGCCGGACCTATCGCGCCGACCATGACGCCACCCATTCGCCGATGTTCC
ACCAGTGCGAGGGGCTGGTCATCGACCGGGGCATCACGCTGGCGCACCTGAAAGGGTGCCTGACCGACTTCCTGCGCGCC
TATTTCGGCAATCCCGACCTGCCGGTGCGCTTCCGGGCGTCGTATTTCCCCTTCACCGAGCCGTCGATGGAGGTCGATAT
CGGCTGGTCGCGCAAGAGCGGCGAGATCGGCGGCGGCGACGACTGGCTGGAGGTCCTGGGCTCGGGCATGGTGCATCCGC
GCGTGCTGGCCAATTGCGGCCTGGACCCGCGCGAATGGCAGGGCTTCGCCTTCGGGATGGGGATCGAGCGCCTGACGATG
CTGCGCCACGGGATACCCGACCTGCGTTCGTTCTACGAAAGCGATGTGCGCTGGCTGCGCCATTACGGCGCCAGCCCGCT
GTCGCCGGCCCTGCTGCATGAAGGTCTGTGA

Upstream 100 bases:

>100_bases
GCCGCCCCCAGGGGCGGCCCGTTTTGCATTTCTGGGGTTTATTCCGAAAGGCGGAGCCTGTATCCGCCAAGTCCATAATG
TCCGTTCCCAGCGAGGTGCC

Downstream 100 bases:

>100_bases
TCGATGAAGTTCACCCTGTCCTGGCTGCGCGACCATCTGGACACCACCGCCACGACCGACGAGATCTGCGCCAAGCTGAA
CACGATCGGGCTGGAGGTCG

Product: phenylalanyl-tRNA synthetase subunit alpha

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase alpha chain; PheRS

Number of amino acids: Translated: 356; Mature: 355

Protein sequence:

>356_residues
MSDDLETLRQETLAALAAATDRREWDAVRVGTLGKSGRLTALLKELGRMTPEARKLRGAAVNRLRDDLTREIEARGAEIE
SAILNARLAAERVDVTLPVRPESSGAIHPISRTMEEMAAIFGAMGFKVAEGPDIETDWHNFSALNTPDHHPARTDQDTFY
LPAREGTSQERVLRTQTSGVQIRTMLGEEPPIRIIAPGRTYRADHDATHSPMFHQCEGLVIDRGITLAHLKGCLTDFLRA
YFGNPDLPVRFRASYFPFTEPSMEVDIGWSRKSGEIGGGDDWLEVLGSGMVHPRVLANCGLDPREWQGFAFGMGIERLTM
LRHGIPDLRSFYESDVRWLRHYGASPLSPALLHEGL

Sequences:

>Translated_356_residues
MSDDLETLRQETLAALAAATDRREWDAVRVGTLGKSGRLTALLKELGRMTPEARKLRGAAVNRLRDDLTREIEARGAEIE
SAILNARLAAERVDVTLPVRPESSGAIHPISRTMEEMAAIFGAMGFKVAEGPDIETDWHNFSALNTPDHHPARTDQDTFY
LPAREGTSQERVLRTQTSGVQIRTMLGEEPPIRIIAPGRTYRADHDATHSPMFHQCEGLVIDRGITLAHLKGCLTDFLRA
YFGNPDLPVRFRASYFPFTEPSMEVDIGWSRKSGEIGGGDDWLEVLGSGMVHPRVLANCGLDPREWQGFAFGMGIERLTM
LRHGIPDLRSFYESDVRWLRHYGASPLSPALLHEGL
>Mature_355_residues
SDDLETLRQETLAALAAATDRREWDAVRVGTLGKSGRLTALLKELGRMTPEARKLRGAAVNRLRDDLTREIEARGAEIES
AILNARLAAERVDVTLPVRPESSGAIHPISRTMEEMAAIFGAMGFKVAEGPDIETDWHNFSALNTPDHHPARTDQDTFYL
PAREGTSQERVLRTQTSGVQIRTMLGEEPPIRIIAPGRTYRADHDATHSPMFHQCEGLVIDRGITLAHLKGCLTDFLRAY
FGNPDLPVRFRASYFPFTEPSMEVDIGWSRKSGEIGGGDDWLEVLGSGMVHPRVLANCGLDPREWQGFAFGMGIERLTML
RHGIPDLRSFYESDVRWLRHYGASPLSPALLHEGL

Specific function: Unknown

COG id: COG0016

COG function: function code J; Phenylalanyl-tRNA synthetase alpha subunit

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha chain type 1 subfamily

Homologues:

Organism=Homo sapiens, GI4758340, Length=286, Percent_Identity=32.8671328671329, Blast_Score=119, Evalue=3e-27,
Organism=Homo sapiens, GI5729820, Length=268, Percent_Identity=29.8507462686567, Blast_Score=105, Evalue=8e-23,
Organism=Escherichia coli, GI1788007, Length=329, Percent_Identity=55.0151975683891, Blast_Score=374, Evalue=1e-105,
Organism=Caenorhabditis elegans, GI32563657, Length=280, Percent_Identity=32.5, Blast_Score=131, Evalue=5e-31,
Organism=Caenorhabditis elegans, GI17508957, Length=280, Percent_Identity=32.5, Blast_Score=131, Evalue=6e-31,
Organism=Caenorhabditis elegans, GI32566635, Length=261, Percent_Identity=29.8850574712644, Blast_Score=100, Evalue=9e-22,
Organism=Saccharomyces cerevisiae, GI6321087, Length=375, Percent_Identity=29.3333333333333, Blast_Score=138, Evalue=2e-33,
Organism=Saccharomyces cerevisiae, GI6325304, Length=113, Percent_Identity=36.283185840708, Blast_Score=76, Evalue=8e-15,
Organism=Drosophila melanogaster, GI18858079, Length=315, Percent_Identity=29.2063492063492, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI17137424, Length=298, Percent_Identity=26.1744966442953, Blast_Score=92, Evalue=8e-19,

Paralogues:

None

Copy number: 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYFA_GLUDA (A9H167)

Other databases:

- EMBL:   AM889285
- EMBL:   CP001189
- RefSeq:   YP_001603503.1
- RefSeq:   YP_002277438.1
- ProteinModelPortal:   A9H167
- SMR:   A9H167
- GeneID:   5790693
- GeneID:   6976527
- GenomeReviews:   AM889285_GR
- GenomeReviews:   CP001189_GR
- KEGG:   gdj:Gdia_3093
- HOGENOM:   HBG284353
- OMA:   FRASYFP
- ProtClustDB:   PRK00488
- GO:   GO:0005737
- HAMAP:   MF_00281
- InterPro:   IPR006195
- InterPro:   IPR004529
- InterPro:   IPR004188
- InterPro:   IPR022911
- InterPro:   IPR002319
- InterPro:   IPR010978
- PANTHER:   PTHR11538
- TIGRFAMs:   TIGR00468

Pfam domain/function: PF02912 Phe_tRNA-synt_N; PF01409 tRNA-synt_2d; SSF46589 tRNA_binding_arm

EC number: =6.1.1.20

Molecular weight: Translated: 39620; Mature: 39489

Theoretical pI: Translated: 6.10; Mature: 6.10

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDDLETLRQETLAALAAATDRREWDAVRVGTLGKSGRLTALLKELGRMTPEARKLRGAA
CCCHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHCCCHHHHHHHHHH
VNRLRDDLTREIEARGAEIESAILNARLAAERVDVTLPVRPESSGAIHPISRTMEEMAAI
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCHHHHHHHHHHHH
FGAMGFKVAEGPDIETDWHNFSALNTPDHHPARTDQDTFYLPAREGTSQERVLRTQTSGV
HHHHCCEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHCCCCE
QIRTMLGEEPPIRIIAPGRTYRADHDATHSPMFHQCEGLVIDRGITLAHLKGCLTDFLRA
EEEEECCCCCCEEEECCCCCCCCCCCCCCCCHHHHCCCEEEECCCHHHHHHHHHHHHHHH
YFGNPDLPVRFRASYFPFTEPSMEVDIGWSRKSGEIGGGDDWLEVLGSGMVHPRVLANCG
HHCCCCCCEEEEECCCCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHCCCCCHHHHHHCC
LDPREWQGFAFGMGIERLTMLRHGIPDLRSFYESDVRWLRHYGASPLSPALLHEGL
CCHHHCCCEEEHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCC
>Mature Secondary Structure 
SDDLETLRQETLAALAAATDRREWDAVRVGTLGKSGRLTALLKELGRMTPEARKLRGAA
CCHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHCCCHHHHHHHHHH
VNRLRDDLTREIEARGAEIESAILNARLAAERVDVTLPVRPESSGAIHPISRTMEEMAAI
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCHHHHHHHHHHHH
FGAMGFKVAEGPDIETDWHNFSALNTPDHHPARTDQDTFYLPAREGTSQERVLRTQTSGV
HHHHCCEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHCCCCE
QIRTMLGEEPPIRIIAPGRTYRADHDATHSPMFHQCEGLVIDRGITLAHLKGCLTDFLRA
EEEEECCCCCCEEEECCCCCCCCCCCCCCCCHHHHCCCEEEECCCHHHHHHHHHHHHHHH
YFGNPDLPVRFRASYFPFTEPSMEVDIGWSRKSGEIGGGDDWLEVLGSGMVHPRVLANCG
HHCCCCCCEEEEECCCCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHCCCCCHHHHHHCC
LDPREWQGFAFGMGIERLTMLRHGIPDLRSFYESDVRWLRHYGASPLSPALLHEGL
CCHHHCCCEEEHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA