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The map label for this gene is kdtA [H]

Identifier: 162149012

GI number: 162149012

Start: 3332139

End: 3333446

Strand: Direct

Name: kdtA [H]

Synonym: GDI_3242

Alternate gene names: 162149012

Gene position: 3332139-3333446 (Clockwise)

Preceding gene: 162149011

Following gene: 162149013

Centisome position: 84.48

GC content: 75.69

Gene sequence:

>1308_bases
ATGCGCGCCGCCTGCCCCGCCCCGGCCCGGTTCCTGTCGGGCCTGTGGGCCGGCCTTGCCACCGCGCTGGCTCCGGCGCT
GACCCTGCTGCTGCGCCGGCGCCTGCGGCGGGGCAAGGAAGTCCGGGGACGGCTGGACGAACGCAAGGGCCGGTCCGCAC
GCCCCCGCCCGCCCGGGCGCGTCCTGTGGCTGCACGCCGCCAGCGTGGGCGAGACCCTGTCGGTCCTGCCCGTCGTCGCC
GAACTGACGCGGCGGGACGCCACGCTGCATGTGCTGTTCACCACGGCGACCGTCACCGCCGGCGAACTGCTGGACCGCCG
CCGGGCCCAGGCCGCGTGGGGCGCGCGGGTGATCCATCAGTTCGTGCCGCTGGACGTGCCGGGCTGGATGGACCGCTTCC
TGCGCCACTGGCGCCCCGATGCGGCGGCGCTGACCGAAAGCGAGCTATGGCCCAACCTGCTGGAATCCTGCCATCGGGCC
GGCGTCCCCATCGTGCTGCTGAACGCGCGGCTGTCGGACCGCTCGCGCGCGGGATGGAGCCGTCTGCGGGGCCTGGCGGA
ACGCATGCTCGGCCGCTTCGCCTGGATCGCCGCACGATCGGACGAGGACGCGGCGCGGCTGCACGCCCTCGGCGCCACGC
GGGTGGACGTCCCCGGCGACCTGAAGGACGCCGCCCCGCCCCTGCCGGCCGATCCCGCGGAGATCGACCGCCTGCGCGCC
GTCATCGCCGGGCGCCCGGTCTGGCTGGCTGCCTCGACCCATGAGGGGGAGGAAGACCTGATCGCCCAGGCCGATCGGCT
GCTGCGCGACCGCCACCCGGACCTGCTGACGATCATCGTACCCCGCCACCCCGAACGCGGGGTGGAGGTCGCCGAGCTGC
TGGATGGTGCCCCCCGCCGTGCGGCGGGGGCCGAGCCCGGCCCCGCCGACCGGTTCTGGATCTGCGACACGCTGGGCGAA
CTGGGCCTGTTCTACCGGGTGGTGCCGATCGTCTTCCTGGGCAACAGCCTGTCGGCCCCGGGGGGACGGGACGAACGGGG
CGGACACAACCCGCTGGAGCCGGCGCGGTTCGGCGCCGCCCTTGCCTCCGGGCCGCTGATCGCGAATTTTACCGGCGCCT
TCGCCCGGCTGCGGGACGCCGTCGCCATCGTGCCGGACGCGGCCGCGCTGGCGGACTGGGTGGACGGCATGCTGGCCGAC
CCGGCGCGCGCCCGCGATGCCGGATGCCGCGCGGCCCAGGTCGCCAGCCAGGACCAGGGCCTGCCAGGCCGCATCGCCGC
GCGTCTGCTGGCGCTGATGGAGACGTGA

Upstream 100 bases:

>100_bases
TTCGGGCGCGGGCGCTTCGTCTGCGGGGCGCCCATCATCGTCACCCCGCATGATCATTGAAGACGCCGCGACGCGGCTGG
GCGATGCCATCGACCAGGCG

Downstream 100 bases:

>100_bases
TGCACGCGCCCCGCTTCTGGTCCGGGGGCGATGGCGGCTGGCCCGCGCGCCTGCTGGCGCCGGCCGCAGCACTCTACACG
CTGGCCACGGCGCGGCGCAT

Product: 3-deoxy-D-manno-octulosonic-acid transferase

Products: KDO2-lipid IVA; CMP; KDO1-lipid IVA [C]

Alternate protein names: KDO transferase [H]

Number of amino acids: Translated: 435; Mature: 435

Protein sequence:

>435_residues
MRAACPAPARFLSGLWAGLATALAPALTLLLRRRLRRGKEVRGRLDERKGRSARPRPPGRVLWLHAASVGETLSVLPVVA
ELTRRDATLHVLFTTATVTAGELLDRRRAQAAWGARVIHQFVPLDVPGWMDRFLRHWRPDAAALTESELWPNLLESCHRA
GVPIVLLNARLSDRSRAGWSRLRGLAERMLGRFAWIAARSDEDAARLHALGATRVDVPGDLKDAAPPLPADPAEIDRLRA
VIAGRPVWLAASTHEGEEDLIAQADRLLRDRHPDLLTIIVPRHPERGVEVAELLDGAPRRAAGAEPGPADRFWICDTLGE
LGLFYRVVPIVFLGNSLSAPGGRDERGGHNPLEPARFGAALASGPLIANFTGAFARLRDAVAIVPDAAALADWVDGMLAD
PARARDAGCRAAQVASQDQGLPGRIAARLLALMET

Sequences:

>Translated_435_residues
MRAACPAPARFLSGLWAGLATALAPALTLLLRRRLRRGKEVRGRLDERKGRSARPRPPGRVLWLHAASVGETLSVLPVVA
ELTRRDATLHVLFTTATVTAGELLDRRRAQAAWGARVIHQFVPLDVPGWMDRFLRHWRPDAAALTESELWPNLLESCHRA
GVPIVLLNARLSDRSRAGWSRLRGLAERMLGRFAWIAARSDEDAARLHALGATRVDVPGDLKDAAPPLPADPAEIDRLRA
VIAGRPVWLAASTHEGEEDLIAQADRLLRDRHPDLLTIIVPRHPERGVEVAELLDGAPRRAAGAEPGPADRFWICDTLGE
LGLFYRVVPIVFLGNSLSAPGGRDERGGHNPLEPARFGAALASGPLIANFTGAFARLRDAVAIVPDAAALADWVDGMLAD
PARARDAGCRAAQVASQDQGLPGRIAARLLALMET
>Mature_435_residues
MRAACPAPARFLSGLWAGLATALAPALTLLLRRRLRRGKEVRGRLDERKGRSARPRPPGRVLWLHAASVGETLSVLPVVA
ELTRRDATLHVLFTTATVTAGELLDRRRAQAAWGARVIHQFVPLDVPGWMDRFLRHWRPDAAALTESELWPNLLESCHRA
GVPIVLLNARLSDRSRAGWSRLRGLAERMLGRFAWIAARSDEDAARLHALGATRVDVPGDLKDAAPPLPADPAEIDRLRA
VIAGRPVWLAASTHEGEEDLIAQADRLLRDRHPDLLTIIVPRHPERGVEVAELLDGAPRRAAGAEPGPADRFWICDTLGE
LGLFYRVVPIVFLGNSLSAPGGRDERGGHNPLEPARFGAALASGPLIANFTGAFARLRDAVAIVPDAAALADWVDGMLAD
PARARDAGCRAAQVASQDQGLPGRIAARLLALMET

Specific function: Essential step in lipopolysaccharides biosynthesis. Acts at transfer of 3-deoxy-D-mono octulonic acid (KDO) from CMP-KDO to a tetraacyldisaccharide 1,4'-bisphosphate precursor of lipid A (lipid IVA). Transfers two molecules of KDO to lipid IVA [H]

COG id: COG1519

COG function: function code M; 3-deoxy-D-manno-octulosonic-acid transferase

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 1 family [H]

Homologues:

Organism=Escherichia coli, GI1790064, Length=435, Percent_Identity=38.6206896551724, Blast_Score=249, Evalue=3e-67,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001296
- InterPro:   IPR007507 [H]

Pfam domain/function: PF00534 Glycos_transf_1; PF04413 Glycos_transf_N [H]

EC number: 2.-.-.- [C]

Molecular weight: Translated: 47107; Mature: 47107

Theoretical pI: Translated: 10.71; Mature: 10.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRAACPAPARFLSGLWAGLATALAPALTLLLRRRLRRGKEVRGRLDERKGRSARPRPPGR
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCC
VLWLHAASVGETLSVLPVVAELTRRDATLHVLFTTATVTAGELLDRRRAQAAWGARVIHQ
EEEEEECCCCCHHHHHHHHHHHHHCCCEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHH
FVPLDVPGWMDRFLRHWRPDAAALTESELWPNLLESCHRAGVPIVLLNARLSDRSRAGWS
HCCCCCCHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHCCCCEEEEECCCCCCHHHHHH
RLRGLAERMLGRFAWIAARSDEDAARLHALGATRVDVPGDLKDAAPPLPADPAEIDRLRA
HHHHHHHHHHHHHHHHEECCCCHHHHHHHHCCCCCCCCCCCHHCCCCCCCCHHHHHHHHH
VIAGRPVWLAASTHEGEEDLIAQADRLLRDRHPDLLTIIVPRHPERGVEVAELLDGAPRR
HHCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHCCCCCC
AAGAEPGPADRFWICDTLGELGLFYRVVPIVFLGNSLSAPGGRDERGGHNPLEPARFGAA
CCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHH
LASGPLIANFTGAFARLRDAVAIVPDAAALADWVDGMLADPARARDAGCRAAQVASQDQG
HHCCCEEEHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHCCCHHHHHHHCCCC
LPGRIAARLLALMET
CCHHHHHHHHHHHCC
>Mature Secondary Structure
MRAACPAPARFLSGLWAGLATALAPALTLLLRRRLRRGKEVRGRLDERKGRSARPRPPGR
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCC
VLWLHAASVGETLSVLPVVAELTRRDATLHVLFTTATVTAGELLDRRRAQAAWGARVIHQ
EEEEEECCCCCHHHHHHHHHHHHHCCCEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHH
FVPLDVPGWMDRFLRHWRPDAAALTESELWPNLLESCHRAGVPIVLLNARLSDRSRAGWS
HCCCCCCHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHCCCCEEEEECCCCCCHHHHHH
RLRGLAERMLGRFAWIAARSDEDAARLHALGATRVDVPGDLKDAAPPLPADPAEIDRLRA
HHHHHHHHHHHHHHHHEECCCCHHHHHHHHCCCCCCCCCCCHHCCCCCCCCHHHHHHHHH
VIAGRPVWLAASTHEGEEDLIAQADRLLRDRHPDLLTIIVPRHPERGVEVAELLDGAPRR
HHCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHCCCCCC
AAGAEPGPADRFWICDTLGELGLFYRVVPIVFLGNSLSAPGGRDERGGHNPLEPARFGAA
CCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHH
LASGPLIANFTGAFARLRDAVAIVPDAAALADWVDGMLADPARARDAGCRAAQVASQDQG
HHCCCEEEHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHCCCHHHHHHHCCCC
LPGRIAARLLALMET
CCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: KDO1-lipid IVA; CMP-3-deoxy-D-manno-octulosonate; lipid IVA [C]

Specific reaction: KDO1-lipid IVA + CMP-3-deoxy-D-manno-octulosonate = KDO2-lipid IVA + CMP CMP-3-deoxy-D-manno-octulosonate + lipid IVA = KDO1-lipid IVA + CMP [C]

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]