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The map label for this gene is cysD [H]

Identifier: 162149006

GI number: 162149006

Start: 3325983

End: 3326777

Strand: Direct

Name: cysD [H]

Synonym: GDI_3236

Alternate gene names: 162149006

Gene position: 3325983-3326777 (Clockwise)

Preceding gene: 162149005

Following gene: 162149007

Centisome position: 84.33

GC content: 64.91

Gene sequence:

>795_bases
ATGGACGATCTCGACCAACTCGAAGCCCAGAGCGTGTATATCCTGCGCGAAGCGTACCGGAAGCTGAAGCCGCTGGCGAT
GCTGTGGTCGCTGGGCAAGGATTCCAACGTCATGCTGTGGCTGGCGCGCAAGGCGTTCCTGGGCCGCGTGCCGTTCCCGG
TGATGCATGTCGATACCGGCAAGAAATTCCCCGAGATGTACCGGTTCCGCGACGAATATGTCCGCAAATGGAACCTGGAA
CTGCTGCTGGGCGACTGCCCGCCGGTCGAGGAGATCGACCCGACCCTGCCGCCGGCCGCCCGCTCCGCCGCGCGCAAGAC
CGCCGGCCTGGCCACGATGATCGAGAAATACAAGCTGGAAGGCGTGATCGCCGGCATCCGCCGCGACGAACAGGCGACCC
GCGCCAAGGAGCGCGTCTTCAGCCCCCGCGGGTCCAGCCACAAGTGGGATGTCCGCAACCAGCCGCCGGAATTCTGGGAC
CAGTACGCCACCCCGCATGAGGAAGGCGTGCATATCCGCGTCCATCCCCTGCTGTCATGGCGCGAGATCGATATCTGGCG
CTATATCGAGCGCGAGGGCATTCCGCTGGTGGATTTGTATTTCTCGAAGAACGGCAAGCGCTATCGCTCGCTGGGCGACC
AGGACATCACCAGCCCGATCGAAAGCGAGGCCGCCACGGTGGCCGAGGTGATCGCGGAACTGCAGACCACCCGCACGTCC
GAGCGCGCCGGCCGCGCCATGGACCATGAATCCGAAGACGCGTTCGAGCGGCTGCGCGTCGCCGGCTATCTGTGA

Upstream 100 bases:

>100_bases
GCGCGCCGGACGCTGGGCCGGCCTGTCGAAGACCGAATGCGGCATCCACGTCTGAACCCACCCCGTAGAGCCCGAAACCC
TGTAAGGTAGCGCCCCCGTC

Downstream 100 bases:

>100_bases
CCCCGGACGGATCGAGACCCGCCATGACTTATGATTCCGCCGCCCGTCAGGACGCGGCCACCCCCATCGTCATCGTCGGC
CATGTCGACCACGGCAAATC

Product: sulfate adenylyltransferase subunit 2

Products: NA

Alternate protein names: ATP-sulfurylase small subunit; Sulfate adenylate transferase; SAT [H]

Number of amino acids: Translated: 264; Mature: 264

Protein sequence:

>264_residues
MDDLDQLEAQSVYILREAYRKLKPLAMLWSLGKDSNVMLWLARKAFLGRVPFPVMHVDTGKKFPEMYRFRDEYVRKWNLE
LLLGDCPPVEEIDPTLPPAARSAARKTAGLATMIEKYKLEGVIAGIRRDEQATRAKERVFSPRGSSHKWDVRNQPPEFWD
QYATPHEEGVHIRVHPLLSWREIDIWRYIEREGIPLVDLYFSKNGKRYRSLGDQDITSPIESEAATVAEVIAELQTTRTS
ERAGRAMDHESEDAFERLRVAGYL

Sequences:

>Translated_264_residues
MDDLDQLEAQSVYILREAYRKLKPLAMLWSLGKDSNVMLWLARKAFLGRVPFPVMHVDTGKKFPEMYRFRDEYVRKWNLE
LLLGDCPPVEEIDPTLPPAARSAARKTAGLATMIEKYKLEGVIAGIRRDEQATRAKERVFSPRGSSHKWDVRNQPPEFWD
QYATPHEEGVHIRVHPLLSWREIDIWRYIEREGIPLVDLYFSKNGKRYRSLGDQDITSPIESEAATVAEVIAELQTTRTS
ERAGRAMDHESEDAFERLRVAGYL
>Mature_264_residues
MDDLDQLEAQSVYILREAYRKLKPLAMLWSLGKDSNVMLWLARKAFLGRVPFPVMHVDTGKKFPEMYRFRDEYVRKWNLE
LLLGDCPPVEEIDPTLPPAARSAARKTAGLATMIEKYKLEGVIAGIRRDEQATRAKERVFSPRGSSHKWDVRNQPPEFWD
QYATPHEEGVHIRVHPLLSWREIDIWRYIEREGIPLVDLYFSKNGKRYRSLGDQDITSPIESEAATVAEVIAELQTTRTS
ERAGRAMDHESEDAFERLRVAGYL

Specific function: First step in the sulfate activation pathway. This reaction occurs early in the reductive branch of the cysteine biosynthetic pathway. [C]

COG id: COG0175

COG function: function code EH; 3'-phosphoadenosine 5'-phosphosulfate sulfotransferase (PAPS reductase)/FAD synthetase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PAPS reductase family. CysD subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789109, Length=299, Percent_Identity=39.1304347826087, Blast_Score=201, Evalue=6e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002500
- InterPro:   IPR014729
- InterPro:   IPR011784 [H]

Pfam domain/function: PF01507 PAPS_reduct [H]

EC number: =2.7.7.4 [H]

Molecular weight: Translated: 30573; Mature: 30573

Theoretical pI: Translated: 6.81; Mature: 6.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDDLDQLEAQSVYILREAYRKLKPLAMLWSLGKDSNVMLWLARKAFLGRVPFPVMHVDTG
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEHHHHHCCCCCCEEEECCC
KKFPEMYRFRDEYVRKWNLELLLGDCPPVEEIDPTLPPAARSAARKTAGLATMIEKYKLE
CCCHHHHHHHHHHHHHHCEEEEECCCCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
GVIAGIRRDEQATRAKERVFSPRGSSHKWDVRNQPPEFWDQYATPHEEGVHIRVHPLLSW
HHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCEEEEEECCCC
REIDIWRYIEREGIPLVDLYFSKNGKRYRSLGDQDITSPIESEAATVAEVIAELQTTRTS
HHHHHHHHHHHCCCCEEEEEECCCCHHHHHCCCHHHCCHHHHHHHHHHHHHHHHHHHHHH
ERAGRAMDHESEDAFERLRVAGYL
HHHCCCCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MDDLDQLEAQSVYILREAYRKLKPLAMLWSLGKDSNVMLWLARKAFLGRVPFPVMHVDTG
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEHHHHHCCCCCCEEEECCC
KKFPEMYRFRDEYVRKWNLELLLGDCPPVEEIDPTLPPAARSAARKTAGLATMIEKYKLE
CCCHHHHHHHHHHHHHHCEEEEECCCCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
GVIAGIRRDEQATRAKERVFSPRGSSHKWDVRNQPPEFWDQYATPHEEGVHIRVHPLLSW
HHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCEEEEEECCCC
REIDIWRYIEREGIPLVDLYFSKNGKRYRSLGDQDITSPIESEAATVAEVIAELQTTRTS
HHHHHHHHHHHCCCCEEEEEECCCCHHHHHCCCHHHCCHHHHHHHHHHHHHHHHHHHHHH
ERAGRAMDHESEDAFERLRVAGYL
HHHCCCCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA