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The map label for this gene is aes [H]

Identifier: 162148950

GI number: 162148950

Start: 3266175

End: 3267086

Strand: Reverse

Name: aes [H]

Synonym: GDI_3180

Alternate gene names: 162148950

Gene position: 3267086-3266175 (Counterclockwise)

Preceding gene: 162148951

Following gene: 162148946

Centisome position: 82.83

GC content: 71.05

Gene sequence:

>912_bases
ATGACCAGCCGGGCGAGGCAGCGGGCGCAGGTGCCGCATACGCTCGAATCCCTGCGACAGGCGCTGGACCGCCCCTGCTT
CCCGCAGGCGCTGTCCAGCCTGCCGCTGCGCCGGGTCCTGGCGGTAAGGGTTCCCGGGGCGACGGAACTGCGCCCCGCGC
GGCTGTATGTTCCCCGTGGCCGCGTCCGGGGTGTGGTCCTGTTCCTGCATGGCGGCGGGTTCGTGCATTGCGGCCTGAAC
TCGCATCATGGAATCTGCTGCCGCCTGGCACGGGCATCAGGCGCCGCCGTGCTGTCCTACGACTATCGCCTGTCGCCGGA
ACACCGTTTTCCGGGCGCGGTCGAGGACTGCCTGGCCGCGCTGGACTGGCTGGCGGGCGAGGCCTGGCGCTGGGGCGGCC
CGATCGCGGTGGCCGGGGACAGCGCGGGCGGCAACCTGGCGGCGGTCCTGGCCCTGCTGGCGCGCGATCGCGGCGGGCCG
GACCTGGCGCTGCAACTGCTGTACTACCCCTCGCTCTATGGCGAGCGGGACGTGCCGTCGCGCCATCGCTATGCGGAGGG
CTACATGCTGACGACGCGACTGATGGAATGGTACGCGCAGCAATATATCCGCACGCCCGACGACCTGCGTCATCCGCTGA
TGGCCCCGATCCATGCCGACTCGCTGGCGGGACTGCCGCCGGCGGTCATCGTGCCCGCGCAGTGCGACCCGCTGCATGAC
GAGGCCACGGGCTATGCCGCCGCCCTGCGCGAAGCGGGCGGCCAGGCGGAGGTGCGCTGCTATCCCGGCACGATCCACGG
CTACCTGAACCTGTATTCGTTCATGCCGTGGGGGAAGGCGGCCATCCGTTTCGGCGGCAAACGCCTGCGCGCGGCGTTTG
CCGGGCAGGACCCGGTCAGATCTGCTGGATAG

Upstream 100 bases:

>100_bases
TGCGCCGCATCAAAAAGAGGCAGATGGAGGCGCGGACATGGATGTCGGACCGGCGGGGCGGACCACGCCCGGATTGGCGG
CGCGGCTGCTGCTGCGGCAC

Downstream 100 bases:

>100_bases
AGGAAAAGTCGAAATCGGACCCCGCCGGGTTCTGCCCCAGGCGGATCGTCGTCGGGCCGGGCATCGAATAGGGCGGGACG
GGCAGGTTGTACGGCGCCGG

Product: lipase protein

Products: alcohol; a carboxylate [C]

Alternate protein names: NA

Number of amino acids: Translated: 303; Mature: 302

Protein sequence:

>303_residues
MTSRARQRAQVPHTLESLRQALDRPCFPQALSSLPLRRVLAVRVPGATELRPARLYVPRGRVRGVVLFLHGGGFVHCGLN
SHHGICCRLARASGAAVLSYDYRLSPEHRFPGAVEDCLAALDWLAGEAWRWGGPIAVAGDSAGGNLAAVLALLARDRGGP
DLALQLLYYPSLYGERDVPSRHRYAEGYMLTTRLMEWYAQQYIRTPDDLRHPLMAPIHADSLAGLPPAVIVPAQCDPLHD
EATGYAAALREAGGQAEVRCYPGTIHGYLNLYSFMPWGKAAIRFGGKRLRAAFAGQDPVRSAG

Sequences:

>Translated_303_residues
MTSRARQRAQVPHTLESLRQALDRPCFPQALSSLPLRRVLAVRVPGATELRPARLYVPRGRVRGVVLFLHGGGFVHCGLN
SHHGICCRLARASGAAVLSYDYRLSPEHRFPGAVEDCLAALDWLAGEAWRWGGPIAVAGDSAGGNLAAVLALLARDRGGP
DLALQLLYYPSLYGERDVPSRHRYAEGYMLTTRLMEWYAQQYIRTPDDLRHPLMAPIHADSLAGLPPAVIVPAQCDPLHD
EATGYAAALREAGGQAEVRCYPGTIHGYLNLYSFMPWGKAAIRFGGKRLRAAFAGQDPVRSAG
>Mature_302_residues
TSRARQRAQVPHTLESLRQALDRPCFPQALSSLPLRRVLAVRVPGATELRPARLYVPRGRVRGVVLFLHGGGFVHCGLNS
HHGICCRLARASGAAVLSYDYRLSPEHRFPGAVEDCLAALDWLAGEAWRWGGPIAVAGDSAGGNLAAVLALLARDRGGPD
LALQLLYYPSLYGERDVPSRHRYAEGYMLTTRLMEWYAQQYIRTPDDLRHPLMAPIHADSLAGLPPAVIVPAQCDPLHDE
ATGYAAALREAGGQAEVRCYPGTIHGYLNLYSFMPWGKAAIRFGGKRLRAAFAGQDPVRSAG

Specific function: Displays esterase activity towards short chain fatty esters (acyl chain length of up to 8 carbons). Able to hydrolyze triacetylglycerol (triacetin) and tributyrylglycerol (tributyrin), but not trioleylglycerol (triolein) or cholesterol oleate. Negatively

COG id: COG0657

COG function: function code I; Esterase/lipase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'GDXG' lipolytic enzyme family [H]

Homologues:

Organism=Homo sapiens, GI157041239, Length=145, Percent_Identity=33.7931034482759, Blast_Score=75, Evalue=8e-14,
Organism=Homo sapiens, GI206597554, Length=295, Percent_Identity=27.4576271186441, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI68299767, Length=182, Percent_Identity=31.3186813186813, Blast_Score=69, Evalue=4e-12,
Organism=Homo sapiens, GI21328446, Length=97, Percent_Identity=41.2371134020619, Blast_Score=68, Evalue=9e-12,
Organism=Escherichia coli, GI1786682, Length=257, Percent_Identity=32.6848249027237, Blast_Score=112, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI17567059, Length=302, Percent_Identity=25.8278145695364, Blast_Score=89, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17540028, Length=158, Percent_Identity=36.0759493670886, Blast_Score=86, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI72001146, Length=128, Percent_Identity=37.5, Blast_Score=81, Evalue=8e-16,
Organism=Caenorhabditis elegans, GI71996133, Length=129, Percent_Identity=38.7596899224806, Blast_Score=80, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013094
- InterPro:   IPR002168 [H]

Pfam domain/function: PF07859 Abhydrolase_3 [H]

EC number: 3.1.1.- [C]

Molecular weight: Translated: 33023; Mature: 32892

Theoretical pI: Translated: 9.32; Mature: 9.32

Prosite motif: PS01174 LIPASE_GDXG_SER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSRARQRAQVPHTLESLRQALDRPCFPQALSSLPLRRVLAVRVPGATELRPARLYVPRG
CCCHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHCCHHHHHEEECCCCCCCCCEEEECCCC
RVRGVVLFLHGGGFVHCGLNSHHGICCRLARASGAAVLSYDYRLSPEHRFPGAVEDCLAA
CCCEEEEEEECCCEEEECCCCCCCCEEEEECCCCCEEEEEEEEECCCCCCCCHHHHHHHH
LDWLAGEAWRWGGPIAVAGDSAGGNLAAVLALLARDRGGPDLALQLLYYPSLYGERDVPS
HHHHHCCCEECCCCEEEECCCCCCHHHHHHHHHHHCCCCHHHEEHEECCCCCCCCCCCCC
RHRYAEGYMLTTRLMEWYAQQYIRTPDDLRHPLMAPIHADSLAGLPPAVIVPAQCDPLHD
CHHHHCCCHHHHHHHHHHHHHHCCCCHHHCCCCCCCCCCCHHCCCCCEEEECCCCCCCCC
EATGYAAALREAGGQAEVRCYPGTIHGYLNLYSFMPWGKAAIRFGGKRLRAAFAGQDPVR
HHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHCCCCCHHHHHHCHHHHHHHHCCCCCCC
SAG
CCC
>Mature Secondary Structure 
TSRARQRAQVPHTLESLRQALDRPCFPQALSSLPLRRVLAVRVPGATELRPARLYVPRG
CCHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHCCHHHHHEEECCCCCCCCCEEEECCCC
RVRGVVLFLHGGGFVHCGLNSHHGICCRLARASGAAVLSYDYRLSPEHRFPGAVEDCLAA
CCCEEEEEEECCCEEEECCCCCCCCEEEEECCCCCEEEEEEEEECCCCCCCCHHHHHHHH
LDWLAGEAWRWGGPIAVAGDSAGGNLAAVLALLARDRGGPDLALQLLYYPSLYGERDVPS
HHHHHCCCEECCCCEEEECCCCCCHHHHHHHHHHHCCCCHHHEEHEECCCCCCCCCCCCC
RHRYAEGYMLTTRLMEWYAQQYIRTPDDLRHPLMAPIHADSLAGLPPAVIVPAQCDPLHD
CHHHHCCCHHHHHHHHHHHHHHCCCCHHHCCCCCCCCCCCHHCCCCCEEEECCCCCCCCC
EATGYAAALREAGGQAEVRCYPGTIHGYLNLYSFMPWGKAAIRFGGKRLRAAFAGQDPVR
HHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHCCCCCHHHHHHCHHHHHHHHCCCCCCC
SAG
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Carboxylic ester; H2O [C]

Specific reaction: Carboxylic ester + H2O = an alcohol + carboxylate [C]

General reaction: Hydrolase; Acting on ester bonds; Carboxylic ester hydrolases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA