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The map label for this gene is proB [H]

Identifier: 162148944

GI number: 162148944

Start: 3261052

End: 3262200

Strand: Direct

Name: proB [H]

Synonym: GDI_3174

Alternate gene names: 162148944

Gene position: 3261052-3262200 (Clockwise)

Preceding gene: 162148943

Following gene: 162148947

Centisome position: 82.68

GC content: 73.72

Gene sequence:

>1149_bases
GTGACCGCCCCCGAATCCACCGGCCTGCCCGACCTGGGCGCCGCCCGCCGCGTCGTCGTGAAGATCGGCAGCGCGCTGGT
CGTGGATTCGGGGACCGAGCCCGGCCAGGCCGCGCCGCGCGCCGCCTGGCTGGACAGCGTCGCCCGCGACATTGCGGAGC
TTCGGCGCGCCGGCACCGACATCATCGTCGTGTCCTCGGGCGCCATCGCGCTGGCGCGGCACGGGCTGGGACTGACGCGC
CGCACCCTGCGGCTGGAGGAAAAGCAGGCCGCGGCCTCGGTCGGGCAGATCCGCCTGGCCCAGGCCTGGACCGACGCGCT
GTCGGTGCACGGACTGGTCGCCGCGCAATTGCTGCTGACCCCCGACGACACCGAGGACCGGCGCCGCTACCTGAACGCCC
GCGCCACGCTGGACACCCTGCTGGGCCTGGGCTGCGTGCCCGTGATCAACGAGAACGACGCGATCGCCACCACCGAAATC
CGCTTCGGCGACAATGACCGCCTGGCCGCCCGGGTGGCGGAAATGACCGGCGCCGACCAGTTGATCCTGCTGTCGGACAT
CGACGGGCTCTATACCGCCGACCCGCGCATCGACCCCACGGCGCGGCACCTGCCGGTCATCCCCGCCCTGACGGCGGAGA
TCGAGGCCATGGGTGGCGCCCCGCCACCGGGCTATTCCTCGGGCGGGATGCGCACCAAGCTGATGGCGGCGCGCATCGCC
ACCCAGGCCGGCTGCGCGATGGCCATCACGATCGGCAAGGACGTCCACCCGCTGGCCCGGCTGCGCCAGGGGGCGCGCTG
CTCGTGGTTCCTGCCGATGCCGGACGGGCGTTCGGCGCGCAAGCGCTGGATCGCGGGCGGCCTGGCCCCCGCGGGGCGGC
TGGCCATCGATGACGGCGCCCGCCGCGCGCTGGCCACCGGGTCGTCGCTGCTGCCCGCCGGGGTACGCACGGTGGAGGGC
GACTTCGAACGCGGCGACCTGCTGCTGGTCACCGACCTGGACGGGAACGAGATCGCGCGCGGCCTGTCGGCCTACAGCGC
CGCCGACGCCCGCCAGATCGCCGGCCACCGCTCGGACGATATCGAAGGACTTCTGGGCTGGCGCGGCCGGGACGAGATGA
TTCACCGCGACGACCTGGTACTGGGCTGA

Upstream 100 bases:

>100_bases
ACGACGTGCTGCGCCTGCTGCAGGACCGCGTGACCGCGACCCGCGAGGCCGCGCGCGACGCGGCCCCGCCGCAGGCCGCG
GCCGGGCGGGAGGAAACGGC

Downstream 100 bases:

>100_bases
TTACCAAGCTGAAAGACAGGCCTTAGAGTCGTCTCTAGTTCCGGGGCGGCCAGCGCAAGGTGGGTTCCGACCGGGGACGG
CGGTTGCCCCATTCGTCGTC

Product: gamma-glutamyl kinase

Products: NA

Alternate protein names: Gamma-glutamyl kinase; GK [H]

Number of amino acids: Translated: 382; Mature: 381

Protein sequence:

>382_residues
MTAPESTGLPDLGAARRVVVKIGSALVVDSGTEPGQAAPRAAWLDSVARDIAELRRAGTDIIVVSSGAIALARHGLGLTR
RTLRLEEKQAAASVGQIRLAQAWTDALSVHGLVAAQLLLTPDDTEDRRRYLNARATLDTLLGLGCVPVINENDAIATTEI
RFGDNDRLAARVAEMTGADQLILLSDIDGLYTADPRIDPTARHLPVIPALTAEIEAMGGAPPPGYSSGGMRTKLMAARIA
TQAGCAMAITIGKDVHPLARLRQGARCSWFLPMPDGRSARKRWIAGGLAPAGRLAIDDGARRALATGSSLLPAGVRTVEG
DFERGDLLLVTDLDGNEIARGLSAYSAADARQIAGHRSDDIEGLLGWRGRDEMIHRDDLVLG

Sequences:

>Translated_382_residues
MTAPESTGLPDLGAARRVVVKIGSALVVDSGTEPGQAAPRAAWLDSVARDIAELRRAGTDIIVVSSGAIALARHGLGLTR
RTLRLEEKQAAASVGQIRLAQAWTDALSVHGLVAAQLLLTPDDTEDRRRYLNARATLDTLLGLGCVPVINENDAIATTEI
RFGDNDRLAARVAEMTGADQLILLSDIDGLYTADPRIDPTARHLPVIPALTAEIEAMGGAPPPGYSSGGMRTKLMAARIA
TQAGCAMAITIGKDVHPLARLRQGARCSWFLPMPDGRSARKRWIAGGLAPAGRLAIDDGARRALATGSSLLPAGVRTVEG
DFERGDLLLVTDLDGNEIARGLSAYSAADARQIAGHRSDDIEGLLGWRGRDEMIHRDDLVLG
>Mature_381_residues
TAPESTGLPDLGAARRVVVKIGSALVVDSGTEPGQAAPRAAWLDSVARDIAELRRAGTDIIVVSSGAIALARHGLGLTRR
TLRLEEKQAAASVGQIRLAQAWTDALSVHGLVAAQLLLTPDDTEDRRRYLNARATLDTLLGLGCVPVINENDAIATTEIR
FGDNDRLAARVAEMTGADQLILLSDIDGLYTADPRIDPTARHLPVIPALTAEIEAMGGAPPPGYSSGGMRTKLMAARIAT
QAGCAMAITIGKDVHPLARLRQGARCSWFLPMPDGRSARKRWIAGGLAPAGRLAIDDGARRALATGSSLLPAGVRTVEGD
FERGDLLLVTDLDGNEIARGLSAYSAADARQIAGHRSDDIEGLLGWRGRDEMIHRDDLVLG

Specific function: Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5- oxoproline [H]

COG id: COG0263

COG function: function code E; Glutamate 5-kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain [H]

Homologues:

Organism=Homo sapiens, GI21361368, Length=277, Percent_Identity=32.129963898917, Blast_Score=101, Evalue=1e-21,
Organism=Homo sapiens, GI62912457, Length=275, Percent_Identity=32.3636363636364, Blast_Score=100, Evalue=2e-21,
Organism=Escherichia coli, GI1786437, Length=368, Percent_Identity=41.0326086956522, Blast_Score=257, Evalue=1e-69,
Organism=Caenorhabditis elegans, GI17569969, Length=257, Percent_Identity=33.852140077821, Blast_Score=125, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI17569967, Length=257, Percent_Identity=33.852140077821, Blast_Score=125, Evalue=3e-29,
Organism=Saccharomyces cerevisiae, GI6320506, Length=418, Percent_Identity=31.8181818181818, Blast_Score=192, Evalue=5e-50,
Organism=Saccharomyces cerevisiae, GI6321822, Length=423, Percent_Identity=30.2600472813239, Blast_Score=186, Evalue=6e-48,
Organism=Drosophila melanogaster, GI21357643, Length=299, Percent_Identity=30.4347826086957, Blast_Score=98, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001048
- InterPro:   IPR001057
- InterPro:   IPR011529
- InterPro:   IPR005715
- InterPro:   IPR002478
- InterPro:   IPR015947 [H]

Pfam domain/function: PF00696 AA_kinase; PF01472 PUA [H]

EC number: =2.7.2.11 [H]

Molecular weight: Translated: 40338; Mature: 40206

Theoretical pI: Translated: 6.27; Mature: 6.27

Prosite motif: PS50890 PUA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAPESTGLPDLGAARRVVVKIGSALVVDSGTEPGQAAPRAAWLDSVARDIAELRRAGTD
CCCCCCCCCCCCHHHHHHHHEECCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCC
IIVVSSGAIALARHGLGLTRRTLRLEEKQAAASVGQIRLAQAWTDALSVHGLVAAQLLLT
EEEEECCCCEEHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEC
PDDTEDRRRYLNARATLDTLLGLGCVPVINENDAIATTEIRFGDNDRLAARVAEMTGADQ
CCCCHHHHHHHHHHHHHHHHHCCCCEEEECCCCCEEEEEEEECCCCHHHHHHHHHCCCCC
LILLSDIDGLYTADPRIDPTARHLPVIPALTAEIEAMGGAPPPGYSSGGMRTKLMAARIA
EEEEECCCCCEECCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHH
TQAGCAMAITIGKDVHPLARLRQGARCSWFLPMPDGRSARKRWIAGGLAPAGRLAIDDGA
HHCCCEEEEEECCCCCHHHHHHCCCCEEEEEECCCCCHHHHHHHHCCCCCCCCEEECCCC
RRALATGSSLLPAGVRTVEGDFERGDLLLVTDLDGNEIARGLSAYSAADARQIAGHRSDD
HHHHHCCCCCCCCCCEEECCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCC
IEGLLGWRGRDEMIHRDDLVLG
CHHHHCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TAPESTGLPDLGAARRVVVKIGSALVVDSGTEPGQAAPRAAWLDSVARDIAELRRAGTD
CCCCCCCCCCCHHHHHHHHEECCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCC
IIVVSSGAIALARHGLGLTRRTLRLEEKQAAASVGQIRLAQAWTDALSVHGLVAAQLLLT
EEEEECCCCEEHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEC
PDDTEDRRRYLNARATLDTLLGLGCVPVINENDAIATTEIRFGDNDRLAARVAEMTGADQ
CCCCHHHHHHHHHHHHHHHHHCCCCEEEECCCCCEEEEEEEECCCCHHHHHHHHHCCCCC
LILLSDIDGLYTADPRIDPTARHLPVIPALTAEIEAMGGAPPPGYSSGGMRTKLMAARIA
EEEEECCCCCEECCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHH
TQAGCAMAITIGKDVHPLARLRQGARCSWFLPMPDGRSARKRWIAGGLAPAGRLAIDDGA
HHCCCEEEEEECCCCCHHHHHHCCCCEEEEEECCCCCHHHHHHHHCCCCCCCCEEECCCC
RRALATGSSLLPAGVRTVEGDFERGDLLLVTDLDGNEIARGLSAYSAADARQIAGHRSDD
HHHHHCCCCCCCCCCEEECCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCC
IEGLLGWRGRDEMIHRDDLVLG
CHHHHCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA