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The map label for this gene is iaaA [H]

Identifier: 162148908

GI number: 162148908

Start: 3227931

End: 3229019

Strand: Reverse

Name: iaaA [H]

Synonym: GDI_3138

Alternate gene names: 162148908

Gene position: 3229019-3227931 (Counterclockwise)

Preceding gene: 162148909

Following gene: 162148905

Centisome position: 81.87

GC content: 68.04

Gene sequence:

>1089_bases
GTGGCCTGTCCCGCCGTCCCGCACATAATCCCCCCTTGTTCGTTTCGATTCCGATGTTACCGTCACGGCGCAACGGGAGG
GGCGGGAATGCGCGGATCGAGGGTTCTGTCGGGCGGGATAGCGGCGGCCATGGTGATGACGGCGGCGTCGGTGGTCATGC
GGCCGGTGACGGCGCGCGCCGATACCGTGCCCGTGATGGTCATTCACGGGGGCGCGGGCGTCATCAAGGCCGACATGTCG
CCCGAGCGGCGGAAGATCGTCCTGGCGGCGCTGGCCCACGCCCTGGACGCGGGATATGCCGCCCTGAAGGCGGGCAAGCC
GGCCCCGGATGCGGTGGTGGCGGCCATTCACGTGCTGGAGGACGATCCGAACTTCAATGCCGGCAAGGGGGCGGTCTTCA
CCCATGACGGCCATAACGAAATGGATGCGGCCATCATGGACGGCGCGACCCTGCGGGCCGGCGCGATCGCGGGCGTACAG
CACGTGCGCAACCCGATTTCCCTGGCGCGGGCGGTCATGGACCATTCGCCGCACGTCCTGCTGATCGGCGCGGGGGCCGA
GGCGTTTGCCAGGACGCAGGGGATCGCATTGGTGGATACGTCCTATTTCTGGACCCAGCGCCGCTGGGACCAGCTTCAGC
GCGCGTTGAAGGAGGATGCGGCCCATGCCCAGCACGCGGACGAAACCACGGACCGTCATTTCGGCACGGTGGGCGCCGTT
GCGCTGGACAAGGCGGGGCATCTGGCCGCCGGGACGTCGACGGGCGGCATGACCGACAAATTATGGGGCCGGGTCGGGGA
TTCGCCGCTGATCGGCGCGGGCACATACGCGAATGCGGGCTGTGCCATGTCCGGGACCGGGTGGGGAGAATTCTATATCC
GCACCGTGGCGGCGCACGAGATCTGCATGCGCGTGACGGCGATGCATGATTCGCTGGCCCATGCGGCCGACGACGTCATC
AACCACGAAATTCCGGCGCTGGGTGGAAACGGCGGCGCCATCCTGGTCGATTCGGCCGGCGATACCGACGGGATGTATCG
CGCCTGGGTCGGTCGCGACGGCGTGCCGCACGCCGCGATCTTCCAGTAG

Upstream 100 bases:

>100_bases
GGGCGCGACCCATCTGTTCGAGGAGGAGGTAACGCTGGAGCAGGTCGCGAACCATGCGGTGGGGTGGTTCCTGTCCCATC
TGCGCGCGCCCCTCTAGCCA

Downstream 100 bases:

>100_bases
GGCAGACTTCCAGTAGGGCAGATCGTGATCGGATGGCGTCACGCAACCGCAAGGCGCTCCAGGCCCGAAGGGTCCACGAC
CCCTCGGGCCTTCCTCGTGC

Product: L-asparaginase

Products: NA

Alternate protein names: Beta-aspartyl-peptidase; EcAIII; Isoaspartyl dipeptidase [H]

Number of amino acids: Translated: 362; Mature: 361

Protein sequence:

>362_residues
MACPAVPHIIPPCSFRFRCYRHGATGGAGMRGSRVLSGGIAAAMVMTAASVVMRPVTARADTVPVMVIHGGAGVIKADMS
PERRKIVLAALAHALDAGYAALKAGKPAPDAVVAAIHVLEDDPNFNAGKGAVFTHDGHNEMDAAIMDGATLRAGAIAGVQ
HVRNPISLARAVMDHSPHVLLIGAGAEAFARTQGIALVDTSYFWTQRRWDQLQRALKEDAAHAQHADETTDRHFGTVGAV
ALDKAGHLAAGTSTGGMTDKLWGRVGDSPLIGAGTYANAGCAMSGTGWGEFYIRTVAAHEICMRVTAMHDSLAHAADDVI
NHEIPALGGNGGAILVDSAGDTDGMYRAWVGRDGVPHAAIFQ

Sequences:

>Translated_362_residues
MACPAVPHIIPPCSFRFRCYRHGATGGAGMRGSRVLSGGIAAAMVMTAASVVMRPVTARADTVPVMVIHGGAGVIKADMS
PERRKIVLAALAHALDAGYAALKAGKPAPDAVVAAIHVLEDDPNFNAGKGAVFTHDGHNEMDAAIMDGATLRAGAIAGVQ
HVRNPISLARAVMDHSPHVLLIGAGAEAFARTQGIALVDTSYFWTQRRWDQLQRALKEDAAHAQHADETTDRHFGTVGAV
ALDKAGHLAAGTSTGGMTDKLWGRVGDSPLIGAGTYANAGCAMSGTGWGEFYIRTVAAHEICMRVTAMHDSLAHAADDVI
NHEIPALGGNGGAILVDSAGDTDGMYRAWVGRDGVPHAAIFQ
>Mature_361_residues
ACPAVPHIIPPCSFRFRCYRHGATGGAGMRGSRVLSGGIAAAMVMTAASVVMRPVTARADTVPVMVIHGGAGVIKADMSP
ERRKIVLAALAHALDAGYAALKAGKPAPDAVVAAIHVLEDDPNFNAGKGAVFTHDGHNEMDAAIMDGATLRAGAIAGVQH
VRNPISLARAVMDHSPHVLLIGAGAEAFARTQGIALVDTSYFWTQRRWDQLQRALKEDAAHAQHADETTDRHFGTVGAVA
LDKAGHLAAGTSTGGMTDKLWGRVGDSPLIGAGTYANAGCAMSGTGWGEFYIRTVAAHEICMRVTAMHDSLAHAADDVIN
HEIPALGGNGGAILVDSAGDTDGMYRAWVGRDGVPHAAIFQ

Specific function: May be involved in glutathione, and possibly other peptide, transport, although these results could also be due to polar effects of disruption [H]

COG id: COG1446

COG function: function code E; Asparaginase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Ntn-hydrolase family [H]

Homologues:

Organism=Homo sapiens, GI145275202, Length=283, Percent_Identity=41.3427561837456, Blast_Score=183, Evalue=2e-46,
Organism=Homo sapiens, GI145275200, Length=283, Percent_Identity=41.3427561837456, Blast_Score=183, Evalue=2e-46,
Organism=Homo sapiens, GI98991769, Length=313, Percent_Identity=30.3514376996805, Blast_Score=112, Evalue=5e-25,
Organism=Homo sapiens, GI285002251, Length=213, Percent_Identity=33.3333333333333, Blast_Score=102, Evalue=5e-22,
Organism=Homo sapiens, GI285002253, Length=213, Percent_Identity=30.9859154929577, Blast_Score=86, Evalue=7e-17,
Organism=Escherichia coli, GI1787050, Length=308, Percent_Identity=49.3506493506493, Blast_Score=265, Evalue=5e-72,
Organism=Drosophila melanogaster, GI18921183, Length=295, Percent_Identity=38.3050847457627, Blast_Score=155, Evalue=3e-38,
Organism=Drosophila melanogaster, GI21357893, Length=288, Percent_Identity=32.9861111111111, Blast_Score=114, Evalue=8e-26,
Organism=Drosophila melanogaster, GI24657358, Length=182, Percent_Identity=29.1208791208791, Blast_Score=65, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000246 [H]

Pfam domain/function: PF01112 Asparaginase_2 [H]

EC number: =3.4.19.5 [H]

Molecular weight: Translated: 37582; Mature: 37451

Theoretical pI: Translated: 7.05; Mature: 7.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MACPAVPHIIPPCSFRFRCYRHGATGGAGMRGSRVLSGGIAAAMVMTAASVVMRPVTARA
CCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHCCCC
DTVPVMVIHGGAGVIKADMSPERRKIVLAALAHALDAGYAALKAGKPAPDAVVAAIHVLE
CCEEEEEEECCCCEEEECCCCHHHHHHHHHHHHHHHCCHHHHHCCCCCCHHHHHEEEEEE
DDPNFNAGKGAVFTHDGHNEMDAAIMDGATLRAGAIAGVQHVRNPISLARAVMDHSPHVL
CCCCCCCCCCEEEEECCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEE
LIGAGAEAFARTQGIALVDTSYFWTQRRWDQLQRALKEDAAHAQHADETTDRHFGTVGAV
EEECCHHHHHHHCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCHHHH
ALDKAGHLAAGTSTGGMTDKLWGRVGDSPLIGAGTYANAGCAMSGTGWGEFYIRTVAAHE
EECCCCCEECCCCCCCCCHHHHHCCCCCCEECCCCCCCCCCCCCCCCCHHHHHHHHHHHH
ICMRVTAMHDSLAHAADDVINHEIPALGGNGGAILVDSAGDTDGMYRAWVGRDGVPHAAI
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCCEEEECCCCCCCCHHC
FQ
CC
>Mature Secondary Structure 
ACPAVPHIIPPCSFRFRCYRHGATGGAGMRGSRVLSGGIAAAMVMTAASVVMRPVTARA
CCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHCCCC
DTVPVMVIHGGAGVIKADMSPERRKIVLAALAHALDAGYAALKAGKPAPDAVVAAIHVLE
CCEEEEEEECCCCEEEECCCCHHHHHHHHHHHHHHHCCHHHHHCCCCCCHHHHHEEEEEE
DDPNFNAGKGAVFTHDGHNEMDAAIMDGATLRAGAIAGVQHVRNPISLARAVMDHSPHVL
CCCCCCCCCCEEEEECCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEE
LIGAGAEAFARTQGIALVDTSYFWTQRRWDQLQRALKEDAAHAQHADETTDRHFGTVGAV
EEECCHHHHHHHCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCHHHH
ALDKAGHLAAGTSTGGMTDKLWGRVGDSPLIGAGTYANAGCAMSGTGWGEFYIRTVAAHE
EECCCCCEECCCCCCCCCHHHHHCCCCCCEECCCCCCCCCCCCCCCCCHHHHHHHHHHHH
ICMRVTAMHDSLAHAADDVINHEIPALGGNGGAILVDSAGDTDGMYRAWVGRDGVPHAAI
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCCEEEECCCCCCCCHHC
FQ
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503; 3045084; 7984428 [H]