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The map label for this gene is gluQ [H]

Identifier: 162148897

GI number: 162148897

Start: 3218019

End: 3218969

Strand: Reverse

Name: gluQ [H]

Synonym: GDI_3127

Alternate gene names: 162148897

Gene position: 3218969-3218019 (Counterclockwise)

Preceding gene: 162148899

Following gene: 162148896

Centisome position: 81.61

GC content: 73.08

Gene sequence:

>951_bases
ATGCCCGCGTCCGGCACAGCGGGCCCGGGCGGGGAGGGCATTCATGACGGCATCATCCTGTTTTCGTGGGGAAAGACCGT
CACGACACGCTTCGCCCCCAGCCCCACCGGGTACCTGCATTTGGGTCACGCGGCGTCGGCGCTGACGGCCTGGCGCCATG
CGCGCGAATCGGATGGCCGTTTCCTGCTGCGGATCGAGGATATCGACCCCGGCCGCTGCCGCCCGGACTACACGGATGCG
ATCGTCGAGGACCTGGCCTGGCTGGGCATCCTCCCGGACGGCGCCATCCGGCTTCAGTCCGACCATATGGCCGATTATCG
TCGCGTGCTGGACGCGCTGGGGCAGCGCGGGCTGCTCTATCCGTGTTTCTGCACCCGCGCCGACATCCGGCGCGAGGCCG
CCGAGGCCGCGCACGCGCCCCATCGCGCCCCGGACGGCACGCTGGCCTATGGCGGGACCTGCCGGGCGCTGTTGGCGGCG
GAGCGGGCCGACCGGATCGCGGCAGGGCAGGCGCATGTGCTGCGCCTGGACATGGCCCGGGCGGTGCACCAGGCGGGCAG
CGGGATCATGACGTGGCACGAGCGGGGGCACGCGCGCGGGCAGGGCGTGCTGTCCGCGCCCGTCGCGCCGTTCGGCGACG
TCGTCCTGGCACGCAAGGACTGCCCGGCCTCCTATCATCTGTGCGTCACGCATGACGACGCGCTGCAGGGCGTCAGCCTG
GTGACCCGGGGCGAGGATCTGCGCCCCGCGACGGCCGTGCATCGCCTGCTGCAGATCCTTATGGGCTGGCCCGCGCCGGA
ATACGCCCATCATCCGCTGCTGCTGGACGCCGACGGCCGCCGCCTGGCCAAGCGCGACCGGGCGCAGACCCTGCGTGCGC
TGCGCGAGGCCGGACAGAGCGCCGCCGCCGTGCGCGCTGCGGCCATGGGGGGCGCCCCCCTTCCCATCTGA

Upstream 100 bases:

>100_bases
CACCATCATCAGGCGTTGTACTCAATAACCGAACGCCGAATGATATGCGCCGTTGAGAACCTGCCATCTATATCGCAAAC
ATTCCCCGATATCGCAAGGG

Downstream 100 bases:

>100_bases
GAACGGCATCGGTGGCGTGCGCGCGGCGCGGATCAGCGGTTTACCTCGCGCTGCGCTTCGCATTACGGTGGCTTCCGCGT
CGGGCCCGACAGGGGCGCGC

Product: glutamyl-Q tRNA(Asp) synthetase

Products: AMP; diphosphate; L-glutamyl-tRNA(Glu)

Alternate protein names: Glu-Q-RSs [H]

Number of amino acids: Translated: 316; Mature: 315

Protein sequence:

>316_residues
MPASGTAGPGGEGIHDGIILFSWGKTVTTRFAPSPTGYLHLGHAASALTAWRHARESDGRFLLRIEDIDPGRCRPDYTDA
IVEDLAWLGILPDGAIRLQSDHMADYRRVLDALGQRGLLYPCFCTRADIRREAAEAAHAPHRAPDGTLAYGGTCRALLAA
ERADRIAAGQAHVLRLDMARAVHQAGSGIMTWHERGHARGQGVLSAPVAPFGDVVLARKDCPASYHLCVTHDDALQGVSL
VTRGEDLRPATAVHRLLQILMGWPAPEYAHHPLLLDADGRRLAKRDRAQTLRALREAGQSAAAVRAAAMGGAPLPI

Sequences:

>Translated_316_residues
MPASGTAGPGGEGIHDGIILFSWGKTVTTRFAPSPTGYLHLGHAASALTAWRHARESDGRFLLRIEDIDPGRCRPDYTDA
IVEDLAWLGILPDGAIRLQSDHMADYRRVLDALGQRGLLYPCFCTRADIRREAAEAAHAPHRAPDGTLAYGGTCRALLAA
ERADRIAAGQAHVLRLDMARAVHQAGSGIMTWHERGHARGQGVLSAPVAPFGDVVLARKDCPASYHLCVTHDDALQGVSL
VTRGEDLRPATAVHRLLQILMGWPAPEYAHHPLLLDADGRRLAKRDRAQTLRALREAGQSAAAVRAAAMGGAPLPI
>Mature_315_residues
PASGTAGPGGEGIHDGIILFSWGKTVTTRFAPSPTGYLHLGHAASALTAWRHARESDGRFLLRIEDIDPGRCRPDYTDAI
VEDLAWLGILPDGAIRLQSDHMADYRRVLDALGQRGLLYPCFCTRADIRREAAEAAHAPHRAPDGTLAYGGTCRALLAAE
RADRIAAGQAHVLRLDMARAVHQAGSGIMTWHERGHARGQGVLSAPVAPFGDVVLARKDCPASYHLCVTHDDALQGVSLV
TRGEDLRPATAVHRLLQILMGWPAPEYAHHPLLLDADGRRLAKRDRAQTLRALREAGQSAAAVRAAAMGGAPLPI

Specific function: Catalyzes the tRNA-independent activation of glutamate in presence of ATP and the subsequent transfer of glutamate onto a tRNA(Asp). Glutamate is transferred on the 2-amino-5-(4,5- dihydroxy-2-cyclopenten-1-yl) moiety of the queuosine in the wobble positi

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family. GluQ subfamily [H]

Homologues:

Organism=Homo sapiens, GI134288884, Length=271, Percent_Identity=33.5793357933579, Blast_Score=139, Evalue=3e-33,
Organism=Homo sapiens, GI62241042, Length=116, Percent_Identity=31.8965517241379, Blast_Score=69, Evalue=7e-12,
Organism=Escherichia coli, GI1788743, Length=274, Percent_Identity=32.4817518248175, Blast_Score=125, Evalue=2e-30,
Organism=Escherichia coli, GI226510925, Length=264, Percent_Identity=33.7121212121212, Blast_Score=121, Evalue=6e-29,
Organism=Escherichia coli, GI1786895, Length=105, Percent_Identity=36.1904761904762, Blast_Score=70, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI71993394, Length=274, Percent_Identity=28.8321167883212, Blast_Score=108, Evalue=5e-24,
Organism=Saccharomyces cerevisiae, GI6324540, Length=275, Percent_Identity=31.6363636363636, Blast_Score=132, Evalue=1e-31,
Organism=Saccharomyces cerevisiae, GI6324742, Length=108, Percent_Identity=35.1851851851852, Blast_Score=82, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6321192, Length=83, Percent_Identity=42.1686746987952, Blast_Score=70, Evalue=6e-13,
Organism=Drosophila melanogaster, GI24665321, Length=299, Percent_Identity=33.7792642140468, Blast_Score=143, Evalue=1e-34,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR022380
- InterPro:   IPR000924
- InterPro:   IPR020058
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00749 tRNA-synt_1c [H]

EC number: 6.1.1.17

Molecular weight: Translated: 33919; Mature: 33788

Theoretical pI: Translated: 8.19; Mature: 8.19

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPASGTAGPGGEGIHDGIILFSWGKTVTTRFAPSPTGYLHLGHAASALTAWRHARESDGR
CCCCCCCCCCCCCCCCCEEEEECCCEEEEEECCCCCCEEEECHHHHHHHHHHHHCCCCCE
FLLRIEDIDPGRCRPDYTDAIVEDLAWLGILPDGAIRLQSDHMADYRRVLDALGQRGLLY
EEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEECCHHHHHHHHHHHHHCCCCCEE
PCFCTRADIRREAAEAAHAPHRAPDGTLAYGGTCRALLAAERADRIAAGQAHVLRLDMAR
EHHHCHHHHHHHHHHHHCCCCCCCCCCEEECCHHHHHHHHHHHHHHHCCCHHHHHHHHHH
AVHQAGSGIMTWHERGHARGQGVLSAPVAPFGDVVLARKDCPASYHLCVTHDDALQGVSL
HHHHCCCCEEEHHHCCCCCCCCEEECCCCCCCCEEEEECCCCCCEEEEEECCHHHCCCEE
VTRGEDLRPATAVHRLLQILMGWPAPEYAHHPLLLDADGRRLAKRDRAQTLRALREAGQS
EECCCCCCHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHCCCH
AAAVRAAAMGGAPLPI
HHHHHHHHCCCCCCCC
>Mature Secondary Structure 
PASGTAGPGGEGIHDGIILFSWGKTVTTRFAPSPTGYLHLGHAASALTAWRHARESDGR
CCCCCCCCCCCCCCCCEEEEECCCEEEEEECCCCCCEEEECHHHHHHHHHHHHCCCCCE
FLLRIEDIDPGRCRPDYTDAIVEDLAWLGILPDGAIRLQSDHMADYRRVLDALGQRGLLY
EEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEECCHHHHHHHHHHHHHCCCCCEE
PCFCTRADIRREAAEAAHAPHRAPDGTLAYGGTCRALLAAERADRIAAGQAHVLRLDMAR
EHHHCHHHHHHHHHHHHCCCCCCCCCCEEECCHHHHHHHHHHHHHHHCCCHHHHHHHHHH
AVHQAGSGIMTWHERGHARGQGVLSAPVAPFGDVVLARKDCPASYHLCVTHDDALQGVSL
HHHHCCCCEEEHHHCCCCCCCCEEECCCCCCCCEEEEECCCCCCEEEEEECCHHHCCCEE
VTRGEDLRPATAVHRLLQILMGWPAPEYAHHPLLLDADGRRLAKRDRAQTLRALREAGQS
EECCCCCCHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHCCCH
AAAVRAAAMGGAPLPI
HHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-glutamate; tRNA(Glu)

Specific reaction: ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu)

General reaction: Aminoacylation; Esterification [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA