Click here to switch to the map view.

The map label for this gene is rnhA [H]

Identifier: 162148870

GI number: 162148870

Start: 3193824

End: 3194333

Strand: Reverse

Name: rnhA [H]

Synonym: GDI_3100

Alternate gene names: 162148870

Gene position: 3194333-3193824 (Counterclockwise)

Preceding gene: 162148871

Following gene: 162148869

Centisome position: 80.99

GC content: 70.39

Gene sequence:

>510_bases
ATGGCCTCTGACCCCCACCACCCGCCCGAGGCGGATGCCGAAACCGACCTCGCCCTGGTCGAGATCTGGACCGATGGCGG
CTGCAAGCCCAATCCCGGCCCCGGCGGCTGGGCGGTGCTGCTGAAATGCCGCGGCACCGAGCGCGAGTTGTCGGGCGGCG
AGGCCGAAACCACCAACAACCGCATGGAACTGACGGCGGCGGCCGAGGCGCTGGAGGCCCTGAAGCGGCCCTGCCGCGTG
GTGCTGCATACCGACAGCGAATATGTCCGCAATGGCATCACGCGCTGGCATACCGGCTGGGTGCGCCGGAACTGGCGCAA
CGCCTCGGGCGATCCGGTGGCCAACATGGATCTGTGGCGCCGGCTGCTGGACATCTCCGCCCGCCATGACGTGACCTGGC
GCTGGGTGCGCGGCCATTCGGGCGACGAAAACAACGAACGCGTGGATCGGCTGGCCACCGCCGCCCGCGACGCGCTGGGA
CTCGCCTATCCGGTCCGTGGCCGCCGCTGA

Upstream 100 bases:

>100_bases
TGGGTGAACACCCCGGCGGGGGCCATGGTCACGCGCAAGGACCCGCTGGATTACCTGAAGCGCCTGCGGTTCCACCGCGC
GGCACGGAACATGGCGTCCT

Downstream 100 bases:

>100_bases
CGGCGTGATGCGCGCCCCGGCGATCCGGCTTCAGGATCTCGGTGTCTCGCATGGCGGCCGGGCCGTCTTCTCCGGCCTGG
ACGCGACCCTGCCGGCCGGC

Product: ribonuclease H

Products: NA

Alternate protein names: RNase H [H]

Number of amino acids: Translated: 169; Mature: 168

Protein sequence:

>169_residues
MASDPHHPPEADAETDLALVEIWTDGGCKPNPGPGGWAVLLKCRGTERELSGGEAETTNNRMELTAAAEALEALKRPCRV
VLHTDSEYVRNGITRWHTGWVRRNWRNASGDPVANMDLWRRLLDISARHDVTWRWVRGHSGDENNERVDRLATAARDALG
LAYPVRGRR

Sequences:

>Translated_169_residues
MASDPHHPPEADAETDLALVEIWTDGGCKPNPGPGGWAVLLKCRGTERELSGGEAETTNNRMELTAAAEALEALKRPCRV
VLHTDSEYVRNGITRWHTGWVRRNWRNASGDPVANMDLWRRLLDISARHDVTWRWVRGHSGDENNERVDRLATAARDALG
LAYPVRGRR
>Mature_168_residues
ASDPHHPPEADAETDLALVEIWTDGGCKPNPGPGGWAVLLKCRGTERELSGGEAETTNNRMELTAAAEALEALKRPCRVV
LHTDSEYVRNGITRWHTGWVRRNWRNASGDPVANMDLWRRLLDISARHDVTWRWVRGHSGDENNERVDRLATAARDALGL
AYPVRGRR

Specific function: Endonuclease that specifically degrades the RNA of RNA- DNA hybrids [H]

COG id: COG0328

COG function: function code L; Ribonuclease HI

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 RNase H domain [H]

Homologues:

Organism=Homo sapiens, GI21359816, Length=155, Percent_Identity=33.5483870967742, Blast_Score=73, Evalue=1e-13,
Organism=Escherichia coli, GI1786408, Length=139, Percent_Identity=54.6762589928058, Blast_Score=159, Evalue=1e-40,
Organism=Caenorhabditis elegans, GI115532542, Length=136, Percent_Identity=33.0882352941176, Blast_Score=78, Evalue=2e-15,
Organism=Drosophila melanogaster, GI45552509, Length=153, Percent_Identity=32.0261437908497, Blast_Score=74, Evalue=4e-14,
Organism=Drosophila melanogaster, GI17137476, Length=153, Percent_Identity=32.0261437908497, Blast_Score=74, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022892
- InterPro:   IPR012337
- InterPro:   IPR002156 [H]

Pfam domain/function: PF00075 RnaseH [H]

EC number: =3.1.26.4 [H]

Molecular weight: Translated: 18953; Mature: 18822

Theoretical pI: Translated: 7.13; Mature: 7.13

Prosite motif: PS50879 RNASE_H

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASDPHHPPEADAETDLALVEIWTDGGCKPNPGPGGWAVLLKCRGTERELSGGEAETTNN
CCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCC
RMELTAAAEALEALKRPCRVVLHTDSEYVRNGITRWHTGWVRRNWRNASGDPVANMDLWR
EEEHHHHHHHHHHHHHCCEEEEECCHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCHHHHH
RLLDISARHDVTWRWVRGHSGDENNERVDRLATAARDALGLAYPVRGRR
HHHHCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
ASDPHHPPEADAETDLALVEIWTDGGCKPNPGPGGWAVLLKCRGTERELSGGEAETTNN
CCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCC
RMELTAAAEALEALKRPCRVVLHTDSEYVRNGITRWHTGWVRRNWRNASGDPVANMDLWR
EEEHHHHHHHHHHHHHCCEEEEECCHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCHHHHH
RLLDISARHDVTWRWVRGHSGDENNERVDRLATAARDALGLAYPVRGRR
HHHHCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA