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The map label for this gene is iscA [H]

Identifier: 162148824

GI number: 162148824

Start: 3147669

End: 3148049

Strand: Reverse

Name: iscA [H]

Synonym: GDI_3053

Alternate gene names: 162148824

Gene position: 3148049-3147669 (Counterclockwise)

Preceding gene: 162148825

Following gene: 162148819

Centisome position: 79.82

GC content: 67.72

Gene sequence:

>381_bases
ATGACCCTTCCGACCGAACACACGGCCACGCCCTCGCGCCCCGCGCGGCCCCTGCCACCGCTGATGGCGCTGACCGACCG
GGCGGCCGACCGGCTGCGCGTGCTGTACGAAACCGCGCATGCCGGCCAGAAGCTGCGCATCGCGGTCTCGACCAAGGGCT
GCTCGGGCCACGCCTACGACATGAACTTCGTCGATGCCGCCGGCCCCGGGGACGAGGTCGTGACCGACAAGGGCGTGACC
GTGCTGGTGGACCGCAAGGCGACGCTGTTCCTGATCGGCACGACGATGGATTACGAGACCAGACAGCTCGAATCCGGCTT
CACCTTCAGCAATCCCAACGAAAAGGGACGCTGCGGCTGCGGCGAGAGCTTCCACGTCTGA

Upstream 100 bases:

>100_bases
ATGTCGAAATCGTCTGGGACCCGCCATGGGACATGTCGCGCATGAGCGACGATGCCCGCCTGGCCCTGAACATGTTCTGA
TCATTCCCAGGAGGACGCCT

Downstream 100 bases:

>100_bases
TCCCGGGTCCCCGAGAGTCAGCGGAAAGAGCCCGGCCTGGCGGGCCTGCTGCAGGTCCGGCGCGCCTGCCAGGTCCAGCA
GCAGATTCGGTCCCCTCCTG

Product: iron-binding protein iscA

Products: NA

Alternate protein names: Iron-sulfur cluster assembly protein [H]

Number of amino acids: Translated: 126; Mature: 125

Protein sequence:

>126_residues
MTLPTEHTATPSRPARPLPPLMALTDRAADRLRVLYETAHAGQKLRIAVSTKGCSGHAYDMNFVDAAGPGDEVVTDKGVT
VLVDRKATLFLIGTTMDYETRQLESGFTFSNPNEKGRCGCGESFHV

Sequences:

>Translated_126_residues
MTLPTEHTATPSRPARPLPPLMALTDRAADRLRVLYETAHAGQKLRIAVSTKGCSGHAYDMNFVDAAGPGDEVVTDKGVT
VLVDRKATLFLIGTTMDYETRQLESGFTFSNPNEKGRCGCGESFHV
>Mature_125_residues
TLPTEHTATPSRPARPLPPLMALTDRAADRLRVLYETAHAGQKLRIAVSTKGCSGHAYDMNFVDAAGPGDEVVTDKGVTV
LVDRKATLFLIGTTMDYETRQLESGFTFSNPNEKGRCGCGESFHV

Specific function: Is able to transfer iron-sulfur clusters to apo- ferredoxin. Multiple cycles of [2Fe2S] cluster formation and transfer are observed, suggesting that iscA acts catalytically. Recruits intracellular free iron so as to provide iron for the assembly of transi

COG id: COG0316

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hesB/iscA family [H]

Homologues:

Organism=Homo sapiens, GI52426768, Length=118, Percent_Identity=38.135593220339, Blast_Score=97, Evalue=4e-21,
Organism=Escherichia coli, GI1788877, Length=105, Percent_Identity=49.5238095238095, Blast_Score=102, Evalue=6e-24,
Organism=Escherichia coli, GI1786351, Length=107, Percent_Identity=37.3831775700935, Blast_Score=74, Evalue=3e-15,
Organism=Escherichia coli, GI1787974, Length=105, Percent_Identity=32.3809523809524, Blast_Score=60, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI25146411, Length=113, Percent_Identity=40.7079646017699, Blast_Score=96, Evalue=7e-21,
Organism=Caenorhabditis elegans, GI193209025, Length=105, Percent_Identity=42.8571428571429, Blast_Score=95, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6323001, Length=106, Percent_Identity=41.5094339622642, Blast_Score=94, Evalue=4e-21,
Organism=Drosophila melanogaster, GI24642263, Length=118, Percent_Identity=38.135593220339, Blast_Score=90, Evalue=3e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000361
- InterPro:   IPR016092
- InterPro:   IPR017870
- InterPro:   IPR011302 [H]

Pfam domain/function: PF01521 Fe-S_biosyn [H]

EC number: NA

Molecular weight: Translated: 13655; Mature: 13523

Theoretical pI: Translated: 6.93; Mature: 6.93

Prosite motif: PS01152 HESB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLPTEHTATPSRPARPLPPLMALTDRAADRLRVLYETAHAGQKLRIAVSTKGCSGHAYD
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCEEC
MNFVDAAGPGDEVVTDKGVTVLVDRKATLFLIGTTMDYETRQLESGFTFSNPNEKGRCGC
CCEEECCCCCCCEEECCCCEEEEECCEEEEEEECCCCCCHHHHCCCCCCCCCCCCCCCCC
GESFHV
CCCCCC
>Mature Secondary Structure 
TLPTEHTATPSRPARPLPPLMALTDRAADRLRVLYETAHAGQKLRIAVSTKGCSGHAYD
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCEEC
MNFVDAAGPGDEVVTDKGVTVLVDRKATLFLIGTTMDYETRQLESGFTFSNPNEKGRCGC
CCEEECCCCCCCEEECCCCEEEEECCEEEEEEECCCCCCHHHHCCCCCCCCCCCCCCCCC
GESFHV
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12775707 [H]