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The map label for this gene is rumA [C]

Identifier: 162148821

GI number: 162148821

Start: 3145038

End: 3146318

Strand: Direct

Name: rumA [C]

Synonym: GDI_3050

Alternate gene names: 162148821

Gene position: 3145038-3146318 (Clockwise)

Preceding gene: 162148820

Following gene: 162148822

Centisome position: 79.74

GC content: 72.99

Gene sequence:

>1281_bases
ATGACGATCGAAGCCGATATCGCCCTTCTGGGCACGGACGGGGACGGCATTGCCCGCACCGCCGAAGGCGACCGCCTGTT
CATCCCCGGCGCGCTGCCGGGCGAGCATGTCCTGGCCGACCGGGTGGACGAACGCACCGCCCACCTGACATCCATCCTGC
GGCCCAGCCCGGACCGGGTGACGCCGCCCTGCCCGCTGTTCGGAACCTGCGGCGGATGCGCGATCCAGCATCTGTCGCTG
GCCGCCGCGCTGGACTGGAAGGCCGGGCTGGTCGTCCATGCGCTGGAGCGCGCCGGATTCGCCGCCCCCGCGATCACCGG
AACGGTCCAGAGCCCGCCCGGATCGCGCCGGCGGATCGACCTGGCGGTCCGGCGCCGGCCGGAGGGCGTATGCATCGGGC
TGCATGCCCGGCGCGGCGACGTGGTGGACCTGACCGCCTGCCCGGTCCTGGAACCGGCGCTGTTCGCCCTGCTCCCGGCC
CTGCGTGCCGTGATGCAGTCGCTGGGGGCTCTGCGGCGCGACGGTGACGCCCTGATCAACATGCTGGATTCAGGCCCCGA
CCTGCTGCTGGCGACCGACGGCCCGCTGGAAGCGGCGGACCGTGCCAAGCTGGCGTCCTTTGCCCGCGACCACCGGATTC
CCCGCATCGCCTGGCGCCGTGCGGGCACGACGGACGTGCCGGAAACCGCCGCCCAGACCGCATCGGTTCGGCATGAACTG
TCCGGCGTCGCCACCATGCCGCCGCCCGGCGCGTTCCTGCAGCCGACGCGCGAGGGCGAGGCCGCGATCATCGCCGCCGT
ACTGGCCGGCCTACCGCGCCGACTGGTGCGGAAGGATACGGTGATCGAACTCTATGCCGGGTGCGGCACGCTGTCCTTCG
CGCTGGGGCAGCACGTGCGCACCCTGGCGTTCGAGGGACAGCCCGACGCGGTCGCGACCCTGCGCCGCGCCACGACGGGC
ACGCGCGTGGTCGCCGAACAGCGCGACCTGAACCGCCAGCCGGTGATGGCGCGCCAGATTGCCGACGCGCGGGCGGTGGT
GCTGGACCCGCCTTATACCGGGGCCGGGGCGCAGATGGCCGAGATCGTCGCCGGCCGGCCGGAGCGAATCGTCTATGTCA
GTTGCAATCCGCAGGCGCTGGGCCGCGACGCCGCCGGACTGCAGGCGGCGGGCTATCGCCTGGACGGCGTGACGATCATC
GACCAGTTCCTGTGGTCGACCGAGGTCGAAGCCGTCTGCGTCTTCACCGCCCCACCCGCCCCCCGCCGCCGGACGCGGTA
A

Upstream 100 bases:

>100_bases
GCGGGGCCCGCCATCGTGCCCGGTGTCGATGAGGTGATCGACGAGACGGCCTGAGAAAGGCCGGGGCCCTGCCCTTCGAA
ACCCCATTCGCCTGGATGAC

Downstream 100 bases:

>100_bases
TCACGGCGCTTTGGGTGACGACAGGGCCTGCCTTGCGCTAGGCTTGACCTGCTGTTCTGCATCAAGACGGTCTGTCCGGC
CGGAGAGCCTGCCGCTGACG

Product: 23S rRNA (uracil-5-)-methyltransferase RumA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 426; Mature: 425

Protein sequence:

>426_residues
MTIEADIALLGTDGDGIARTAEGDRLFIPGALPGEHVLADRVDERTAHLTSILRPSPDRVTPPCPLFGTCGGCAIQHLSL
AAALDWKAGLVVHALERAGFAAPAITGTVQSPPGSRRRIDLAVRRRPEGVCIGLHARRGDVVDLTACPVLEPALFALLPA
LRAVMQSLGALRRDGDALINMLDSGPDLLLATDGPLEAADRAKLASFARDHRIPRIAWRRAGTTDVPETAAQTASVRHEL
SGVATMPPPGAFLQPTREGEAAIIAAVLAGLPRRLVRKDTVIELYAGCGTLSFALGQHVRTLAFEGQPDAVATLRRATTG
TRVVAEQRDLNRQPVMARQIADARAVVLDPPYTGAGAQMAEIVAGRPERIVYVSCNPQALGRDAAGLQAAGYRLDGVTII
DQFLWSTEVEAVCVFTAPPAPRRRTR

Sequences:

>Translated_426_residues
MTIEADIALLGTDGDGIARTAEGDRLFIPGALPGEHVLADRVDERTAHLTSILRPSPDRVTPPCPLFGTCGGCAIQHLSL
AAALDWKAGLVVHALERAGFAAPAITGTVQSPPGSRRRIDLAVRRRPEGVCIGLHARRGDVVDLTACPVLEPALFALLPA
LRAVMQSLGALRRDGDALINMLDSGPDLLLATDGPLEAADRAKLASFARDHRIPRIAWRRAGTTDVPETAAQTASVRHEL
SGVATMPPPGAFLQPTREGEAAIIAAVLAGLPRRLVRKDTVIELYAGCGTLSFALGQHVRTLAFEGQPDAVATLRRATTG
TRVVAEQRDLNRQPVMARQIADARAVVLDPPYTGAGAQMAEIVAGRPERIVYVSCNPQALGRDAAGLQAAGYRLDGVTII
DQFLWSTEVEAVCVFTAPPAPRRRTR
>Mature_425_residues
TIEADIALLGTDGDGIARTAEGDRLFIPGALPGEHVLADRVDERTAHLTSILRPSPDRVTPPCPLFGTCGGCAIQHLSLA
AALDWKAGLVVHALERAGFAAPAITGTVQSPPGSRRRIDLAVRRRPEGVCIGLHARRGDVVDLTACPVLEPALFALLPAL
RAVMQSLGALRRDGDALINMLDSGPDLLLATDGPLEAADRAKLASFARDHRIPRIAWRRAGTTDVPETAAQTASVRHELS
GVATMPPPGAFLQPTREGEAAIIAAVLAGLPRRLVRKDTVIELYAGCGTLSFALGQHVRTLAFEGQPDAVATLRRATTGT
RVVAEQRDLNRQPVMARQIADARAVVLDPPYTGAGAQMAEIVAGRPERIVYVSCNPQALGRDAAGLQAAGYRLDGVTIID
QFLWSTEVEAVCVFTAPPAPRRRTR

Specific function: Could Be A 23s rRNA (Uracil-5-)-Methyltransferase. [C]

COG id: COG2265

COG function: function code J; SAM-dependent methyltransferases related to tRNA (uracil-5-)-methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RNA M5U methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789148, Length=432, Percent_Identity=30.5555555555556, Blast_Score=162, Evalue=3e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016027
- InterPro:   IPR010280 [H]

Pfam domain/function: PF05958 tRNA_U5-meth_tr [H]

EC number: NA

Molecular weight: Translated: 45207; Mature: 45075

Theoretical pI: Translated: 8.02; Mature: 8.02

Prosite motif: PS50926 TRAM ; PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIEADIALLGTDGDGIARTAEGDRLFIPGALPGEHVLADRVDERTAHLTSILRPSPDRV
CEEEEEEEEEECCCCCCEEECCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCC
TPPCPLFGTCGGCAIQHLSLAAALDWKAGLVVHALERAGFAAPAITGTVQSPPGSRRRID
CCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCEECCCCCCCCCCEEEE
LAVRRRPEGVCIGLHARRGDVVDLTACPVLEPALFALLPALRAVMQSLGALRRDGDALIN
EEEECCCCCEEEEEECCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
MLDSGPDLLLATDGPLEAADRAKLASFARDHRIPRIAWRRAGTTDVPETAAQTASVRHEL
HHCCCCCEEEECCCCCCHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCHHHHHHHHHHHHH
SGVATMPPPGAFLQPTREGEAAIIAAVLAGLPRRLVRKDTVIELYAGCGTLSFALGQHVR
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHE
TLAFEGQPDAVATLRRATTGTRVVAEQRDLNRQPVMARQIADARAVVLDPPYTGAGAQMA
EEEECCCCHHHHHHHHHCCCCEEEEHHHCCCCCCHHHHHHCCCCEEEECCCCCCCCHHHH
EIVAGRPERIVYVSCNPQALGRDAAGLQAAGYRLDGVTIIDQFLWSTEVEAVCVFTAPPA
HHHCCCCCEEEEEECCCHHCCCCCCCCCCCCEEECCHHHHHHHHHCCCCCEEEEEECCCC
PRRRTR
CCCCCC
>Mature Secondary Structure 
TIEADIALLGTDGDGIARTAEGDRLFIPGALPGEHVLADRVDERTAHLTSILRPSPDRV
EEEEEEEEEECCCCCCEEECCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCC
TPPCPLFGTCGGCAIQHLSLAAALDWKAGLVVHALERAGFAAPAITGTVQSPPGSRRRID
CCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCEECCCCCCCCCCEEEE
LAVRRRPEGVCIGLHARRGDVVDLTACPVLEPALFALLPALRAVMQSLGALRRDGDALIN
EEEECCCCCEEEEEECCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
MLDSGPDLLLATDGPLEAADRAKLASFARDHRIPRIAWRRAGTTDVPETAAQTASVRHEL
HHCCCCCEEEECCCCCCHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCHHHHHHHHHHHHH
SGVATMPPPGAFLQPTREGEAAIIAAVLAGLPRRLVRKDTVIELYAGCGTLSFALGQHVR
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHE
TLAFEGQPDAVATLRRATTGTRVVAEQRDLNRQPVMARQIADARAVVLDPPYTGAGAQMA
EEEECCCCHHHHHHHHHCCCCEEEEHHHCCCCCCHHHHHHCCCCEEEECCCCCCCCHHHH
EIVAGRPERIVYVSCNPQALGRDAAGLQAAGYRLDGVTIIDQFLWSTEVEAVCVFTAPPA
HHHCCCCCEEEEEECCCHHCCCCCCCCCCCCEEECCHHHHHHHHHCCCCCEEEEEECCCC
PRRRTR
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11756688 [H]