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The map label for this gene is birA [H]

Identifier: 162148798

GI number: 162148798

Start: 3117704

End: 3118486

Strand: Reverse

Name: birA [H]

Synonym: GDI_3027

Alternate gene names: 162148798

Gene position: 3118486-3117704 (Counterclockwise)

Preceding gene: 162148799

Following gene: 162148796

Centisome position: 79.07

GC content: 69.48

Gene sequence:

>783_bases
GTGAACGGCGCGACGCCGGTCGCACCCGTACCGCCGCCGGGGCCGACACCCTGGCGGCTGGAGATTCATGACGAACTGGA
CTCGACGTCCGATACCTGCCTGGCCCGGGCCGGGCAGGGCGAACGCTCCGGCCTGGCCGTCATGGCCCGCCGCCAGACGC
GCGGGCGTGGCAGCCGGGGGCGGGACTGGCAGGACCCGGGCGGGAACCTCGCGTTTTCGGTCTTGCTCCGGCCGGGCGGG
AAAGGGGAATGGCCCGTCGGGCTGTGGCCCTTCATTGCCGGCCTGGCTTTCCACGGGGCGATTGCACCCTGGATTCCCCA
CCCCATATCTCTCCGGCTGAAATGGCCGAACGACCTGCTTCTGGAAGGGCGCAAGGCGGCTGGAATCCTGATCGAGGCCG
GCACGGATTCGTCCGGGCGATTGCTGGTGATCGGGTTCGGCGCCAATCTGCGGGCGGCCCCCGCCATTACGGGACGGGAC
CTGGCCTGTGTCGCCGAATACGGAACGGCGCCCGAGCCGGAGATCGTGGCACGGGCGATCTGCGCCGGACTGGACCATTG
GATGGCGGTTTGTGGCGGGCAGGGGGACGGCCTGGGGTTCGAAGCGGTGCGGCAGGCGTGGCTGGCGCACGCGCACCCCA
TGGGCACAAGGCTTGTGGTGCGCGCTGAAAGCGGGCAGGTAGAAGGTTCGTTCGCTGGTCTGGACGCGCAGGGCATGCTC
CTGCTGCGTTGCGCCGACGGACTGAAACGGATTTCCACCGGCGAAATCCTGCTGCTGGACTAG

Upstream 100 bases:

>100_bases
CCTTCGTCTCGGTGGGCATGGGCATCGCCACCACCGGCTTCCTGCTGGTGCTGGGACCGGTATCCAGCGCGGCGCAGGCG
GCGGCCCAGGCCCTGTTCAG

Downstream 100 bases:

>100_bases
GTGCCGGCGGGATCGACGGTGACAGGCGCGACGCCTGAATTTTTTTGGCCTGACGGCAGGCGGGGACGATCGTCGAGCGG
AGGCGCACCCTTCGGCTCCG

Product: biotin--[acetyl-CoA-carboxylase] synthetase

Products: NA

Alternate protein names: Biotin--protein ligase [H]

Number of amino acids: Translated: 260; Mature: 260

Protein sequence:

>260_residues
MNGATPVAPVPPPGPTPWRLEIHDELDSTSDTCLARAGQGERSGLAVMARRQTRGRGSRGRDWQDPGGNLAFSVLLRPGG
KGEWPVGLWPFIAGLAFHGAIAPWIPHPISLRLKWPNDLLLEGRKAAGILIEAGTDSSGRLLVIGFGANLRAAPAITGRD
LACVAEYGTAPEPEIVARAICAGLDHWMAVCGGQGDGLGFEAVRQAWLAHAHPMGTRLVVRAESGQVEGSFAGLDAQGML
LLRCADGLKRISTGEILLLD

Sequences:

>Translated_260_residues
MNGATPVAPVPPPGPTPWRLEIHDELDSTSDTCLARAGQGERSGLAVMARRQTRGRGSRGRDWQDPGGNLAFSVLLRPGG
KGEWPVGLWPFIAGLAFHGAIAPWIPHPISLRLKWPNDLLLEGRKAAGILIEAGTDSSGRLLVIGFGANLRAAPAITGRD
LACVAEYGTAPEPEIVARAICAGLDHWMAVCGGQGDGLGFEAVRQAWLAHAHPMGTRLVVRAESGQVEGSFAGLDAQGML
LLRCADGLKRISTGEILLLD
>Mature_260_residues
MNGATPVAPVPPPGPTPWRLEIHDELDSTSDTCLARAGQGERSGLAVMARRQTRGRGSRGRDWQDPGGNLAFSVLLRPGG
KGEWPVGLWPFIAGLAFHGAIAPWIPHPISLRLKWPNDLLLEGRKAAGILIEAGTDSSGRLLVIGFGANLRAAPAITGRD
LACVAEYGTAPEPEIVARAICAGLDHWMAVCGGQGDGLGFEAVRQAWLAHAHPMGTRLVVRAESGQVEGSFAGLDAQGML
LLRCADGLKRISTGEILLLD

Specific function: Activates biotin to form biotinyl-5'-adenylate and transfers the biotin moiety to biotin-accepting proteins [H]

COG id: COG0340

COG function: function code H; Biotin-(acetyl-CoA carboxylase) ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the biotin--protein ligase family [H]

Homologues:

Organism=Escherichia coli, GI1790408, Length=238, Percent_Identity=29.8319327731092, Blast_Score=68, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004408
- InterPro:   IPR003142
- InterPro:   IPR004143 [H]

Pfam domain/function: PF02237 BPL_C; PF03099 BPL_LipA_LipB [H]

EC number: =6.3.4.15 [H]

Molecular weight: Translated: 27408; Mature: 27408

Theoretical pI: Translated: 7.10; Mature: 7.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNGATPVAPVPPPGPTPWRLEIHDELDSTSDTCLARAGQGERSGLAVMARRQTRGRGSRG
CCCCCCCCCCCCCCCCCEEEEECCCCCCCCCHHHHHCCCCCCCCEEEEEEHHHCCCCCCC
RDWQDPGGNLAFSVLLRPGGKGEWPVGLWPFIAGLAFHGAIAPWIPHPISLRLKWPNDLL
CCCCCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCEEEEEECCCHHE
LEGRKAAGILIEAGTDSSGRLLVIGFGANLRAAPAITGRDLACVAEYGTAPEPEIVARAI
ECCCCCCEEEEECCCCCCCCEEEEEECCCCEECCCCCCCCEEEEECCCCCCCHHHHHHHH
CAGLDHWMAVCGGQGDGLGFEAVRQAWLAHAHPMGTRLVVRAESGQVEGSFAGLDAQGML
HHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCEEEEEECCCCCCCEEECCCCCCEE
LLRCADGLKRISTGEILLLD
EEEEHHHHHHCCCCCEEEEC
>Mature Secondary Structure
MNGATPVAPVPPPGPTPWRLEIHDELDSTSDTCLARAGQGERSGLAVMARRQTRGRGSRG
CCCCCCCCCCCCCCCCCEEEEECCCCCCCCCHHHHHCCCCCCCCEEEEEEHHHCCCCCCC
RDWQDPGGNLAFSVLLRPGGKGEWPVGLWPFIAGLAFHGAIAPWIPHPISLRLKWPNDLL
CCCCCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCEEEEEECCCHHE
LEGRKAAGILIEAGTDSSGRLLVIGFGANLRAAPAITGRDLACVAEYGTAPEPEIVARAI
ECCCCCCEEEEECCCCCCCCEEEEEECCCCEECCCCCCCCEEEEECCCCCCCHHHHHHHH
CAGLDHWMAVCGGQGDGLGFEAVRQAWLAHAHPMGTRLVVRAESGQVEGSFAGLDAQGML
HHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCEEEEEECCCCCCCEEECCCCCCEE
LLRCADGLKRISTGEILLLD
EEEEHHHHHHCCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8422400 [H]