Click here to switch to the map view.

The map label for this gene is hemB [H]

Identifier: 162148785

GI number: 162148785

Start: 3101448

End: 3102443

Strand: Direct

Name: hemB [H]

Synonym: GDI_3014

Alternate gene names: 162148785

Gene position: 3101448-3102443 (Clockwise)

Preceding gene: 162148784

Following gene: 162148786

Centisome position: 78.63

GC content: 68.78

Gene sequence:

>996_bases
ATGACGCTCGGCCGCTTTCCCGCCACCCGTCCGCGCCGCAACCGCCAGGACGATTTCACCCGACGCCTGGTCGCGGAAAC
GACGCTGACGGTCGACAACCTGATCTGGCCGATCTTCGTCATGGAAGGCACCGACACGCAGACCGAGGTCGCCTCGATGC
CCGGCGTGCGCCGGGTGACGCTGGACCGGCTGGCGGCCCATGTCGAACCGGCGGCGGCGCTGGGCATTCCGGCCCTGGCG
CTGTTCCCGATCACCCCCACCTCCCTGCGCGACGCGGAGGGGACCGAGGCGATGAACCCCGACAACCTGATGTGCCGGGC
GGCGCGCGTGCTGAAGCGTGAATTCCCCGACATGGGCCTGATCGGGGACGTGGCGCTGGACCCCTATACCGACCATGGGC
ATGACGGACTGATCCGCGACGGATATGTCGTCAACGACGAATCCGTCGCCGTCCTGTCGCGGCAGGCGGTCAACCAGGCG
GCGGCCGGCATCGACATCATCGCGCCGTCCGACATGATGGACGGCCGCATCGGCGTCATCCGCCGCGACCTGGACGCGCA
GGGCCTGACCGGCACGCGGATCATGTCCTATGCCGCCAAATATGCCAGCGCGTTCTATGGCCCGTTCCGCGACGCGCTGG
GATCGGGCGGGCTGCTGAAGGGCGACAAGAAGACCTACCAGATGGACCCGGCCAACAGCGACGAGGCCCTGCGCGAGGTG
GAGCAGGACCTGGCCGAGGGGGCGGACATGGTCATGGTCAAGCCCGGGATGCCGTATCTGGACATCATCCGCCGGGTCCA
TGACCGCTTCGCGGTGCCGACCTTCGCGTACCAGGTATCGGGCGAATACGCGATGCTGACCGCAGCCATGGACCATGGCT
GGCTGGACCGCGACCGCACCATGCTGGAAAGCCTGATGGCCTTCCGCCGCGCCGGCGCCAGCGGGGTGCTGACCTATTTC
GCGGTGGACGCCGCACGCCGGCTGCGGGGCGGCTGA

Upstream 100 bases:

>100_bases
CGCCCCGCTGCCCGAAGCTGGCGGGACAGGTGGACGCGGTCCATACTGCCCCACCTGGCCCCGCCCATCTGGGCCCCCGC
CAATTTCGGAGTTCGCTTTC

Downstream 100 bases:

>100_bases
CCCGACCCGGCCGGGGGAACAGCCTCTTTCATCCAGACGTCACAAATCCTCGCTCACGCGGCGGATTGTGATGCAAACCG
GGCCCTGTACCGTTACCATC

Product: delta-aminolevulinic acid dehydratase

Products: NA

Alternate protein names: ALAD; ALADH; Porphobilinogen synthase [H]

Number of amino acids: Translated: 331; Mature: 330

Protein sequence:

>331_residues
MTLGRFPATRPRRNRQDDFTRRLVAETTLTVDNLIWPIFVMEGTDTQTEVASMPGVRRVTLDRLAAHVEPAAALGIPALA
LFPITPTSLRDAEGTEAMNPDNLMCRAARVLKREFPDMGLIGDVALDPYTDHGHDGLIRDGYVVNDESVAVLSRQAVNQA
AAGIDIIAPSDMMDGRIGVIRRDLDAQGLTGTRIMSYAAKYASAFYGPFRDALGSGGLLKGDKKTYQMDPANSDEALREV
EQDLAEGADMVMVKPGMPYLDIIRRVHDRFAVPTFAYQVSGEYAMLTAAMDHGWLDRDRTMLESLMAFRRAGASGVLTYF
AVDAARRLRGG

Sequences:

>Translated_331_residues
MTLGRFPATRPRRNRQDDFTRRLVAETTLTVDNLIWPIFVMEGTDTQTEVASMPGVRRVTLDRLAAHVEPAAALGIPALA
LFPITPTSLRDAEGTEAMNPDNLMCRAARVLKREFPDMGLIGDVALDPYTDHGHDGLIRDGYVVNDESVAVLSRQAVNQA
AAGIDIIAPSDMMDGRIGVIRRDLDAQGLTGTRIMSYAAKYASAFYGPFRDALGSGGLLKGDKKTYQMDPANSDEALREV
EQDLAEGADMVMVKPGMPYLDIIRRVHDRFAVPTFAYQVSGEYAMLTAAMDHGWLDRDRTMLESLMAFRRAGASGVLTYF
AVDAARRLRGG
>Mature_330_residues
TLGRFPATRPRRNRQDDFTRRLVAETTLTVDNLIWPIFVMEGTDTQTEVASMPGVRRVTLDRLAAHVEPAAALGIPALAL
FPITPTSLRDAEGTEAMNPDNLMCRAARVLKREFPDMGLIGDVALDPYTDHGHDGLIRDGYVVNDESVAVLSRQAVNQAA
AGIDIIAPSDMMDGRIGVIRRDLDAQGLTGTRIMSYAAKYASAFYGPFRDALGSGGLLKGDKKTYQMDPANSDEALREVE
QDLAEGADMVMVKPGMPYLDIIRRVHDRFAVPTFAYQVSGEYAMLTAAMDHGWLDRDRTMLESLMAFRRAGASGVLTYFA
VDAARRLRGG

Specific function: Catalyzes an early step in the biosynthesis of tetrapyrroles. Binds two molecules of 5-aminolevulinate per subunit, each at a distinct site, and catalyzes their condensation to form porphobilinogen. Required for nodule development [H]

COG id: COG0113

COG function: function code H; Delta-aminolevulinic acid dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ALADH family [H]

Homologues:

Organism=Homo sapiens, GI189083849, Length=300, Percent_Identity=43.6666666666667, Blast_Score=233, Evalue=1e-61,
Organism=Escherichia coli, GI87081728, Length=318, Percent_Identity=45.9119496855346, Blast_Score=263, Evalue=2e-71,
Organism=Saccharomyces cerevisiae, GI6321398, Length=306, Percent_Identity=41.5032679738562, Blast_Score=217, Evalue=3e-57,
Organism=Drosophila melanogaster, GI21358291, Length=304, Percent_Identity=39.4736842105263, Blast_Score=234, Evalue=8e-62,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001731
- InterPro:   IPR013785 [H]

Pfam domain/function: PF00490 ALAD [H]

EC number: =4.2.1.24 [H]

Molecular weight: Translated: 36288; Mature: 36157

Theoretical pI: Translated: 5.25; Mature: 5.25

Prosite motif: PS00169 D_ALA_DEHYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
5.1 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLGRFPATRPRRNRQDDFTRRLVAETTLTVDNLIWPIFVMEGTDTQTEVASMPGVRRVT
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHEEEEEEECCCCCHHHHHHCCCCHHHH
LDRLAAHVEPAAALGIPALALFPITPTSLRDAEGTEAMNPDNLMCRAARVLKREFPDMGL
HHHHHHHCCCHHHHCCCCEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCC
IGDVALDPYTDHGHDGLIRDGYVVNDESVAVLSRQAVNQAAAGIDIIAPSDMMDGRIGVI
CCCEECCCCCCCCCCCCEECCEEECCCHHHHHHHHHHHHHHCCEEEECCCCCCCCCCEEE
RRDLDAQGLTGTRIMSYAAKYASAFYGPFRDALGSGGLLKGDKKTYQMDPANSDEALREV
EECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCHHHHHHHH
EQDLAEGADMVMVKPGMPYLDIIRRVHDRFAVPTFAYQVSGEYAMLTAAMDHGWLDRDRT
HHHHHCCCCEEEECCCCCHHHHHHHHHHHHCCCCEEEEECCCEEEEEEHHHCCCCCHHHH
MLESLMAFRRAGASGVLTYFAVDAARRLRGG
HHHHHHHHHHCCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TLGRFPATRPRRNRQDDFTRRLVAETTLTVDNLIWPIFVMEGTDTQTEVASMPGVRRVT
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHEEEEEEECCCCCHHHHHHCCCCHHHH
LDRLAAHVEPAAALGIPALALFPITPTSLRDAEGTEAMNPDNLMCRAARVLKREFPDMGL
HHHHHHHCCCHHHHCCCCEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCC
IGDVALDPYTDHGHDGLIRDGYVVNDESVAVLSRQAVNQAAAGIDIIAPSDMMDGRIGVI
CCCEECCCCCCCCCCCCEECCEEECCCHHHHHHHHHHHHHHCCEEEECCCCCCCCCCEEE
RRDLDAQGLTGTRIMSYAAKYASAFYGPFRDALGSGGLLKGDKKTYQMDPANSDEALREV
EECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCHHHHHHHH
EQDLAEGADMVMVKPGMPYLDIIRRVHDRFAVPTFAYQVSGEYAMLTAAMDHGWLDRDRT
HHHHHCCCCEEEECCCCCHHHHHHHHHHHHCCCCEEEEECCCEEEEEEHHHCCCCCHHHH
MLESLMAFRRAGASGVLTYFAVDAARRLRGG
HHHHHHHHHHCCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8226669; 12597275 [H]