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The map label for this gene is menE [H]

Identifier: 162148778

GI number: 162148778

Start: 3094170

End: 3095555

Strand: Reverse

Name: menE [H]

Synonym: GDI_3007

Alternate gene names: 162148778

Gene position: 3095555-3094170 (Counterclockwise)

Preceding gene: 162148779

Following gene: 162148777

Centisome position: 78.48

GC content: 56.71

Gene sequence:

>1386_bases
ATGTTCTGGCAGGACCGCCCGAGCGACGCCGAAGACCGGACATTCCTGCTGGAAGAAGGGCGTCGCTACAGCTACCACGA
CATTTTCCGTTTGGGCGACGCGCTTTTCGCAGATTCCTCAAGGGCTGTCGCGGTCATTCTCTGCGACCGAACCCATGAGA
CCGTTGCCGCATATTTCGGTGCCTTGCGACATGGGGTCGTTCCGCTGCTTCTCGACGGCCACGCGAGATGGCCCACCTTG
TCAAAGACGATCGAGGCGTACGCGCCCCGCTATCTGGTAGGCAACGCGGATGCCGTCCCGGATGGGTATATCCCGACGAA
GCGGCTCGTCTCGAAAGTGCTCTTCGAGCGTGTCGTGCCGATCGACTATTCGTTAAACGAAAATCTGGCGTTGCTTATCC
CGACCTCGGGCTCTACCGGTGACTCGAAATGCGTGCGGCTGACGGGGCGAAACATCGAATCCTGTACCCGGGCAATCGTG
AGCTATCTCGCCATTACAGAAGAGCGCGTCACGGTAAGCCTGCTTCCATTTCATTATTCCTATGGACTTTCCGTACTTCA
CAATACTGCCTTCGTTCGCGGCACGATGTTGTTGACGGAACAGTCTGTTCTGGAAAAAAATCTCTGGCAACGTATCGAAG
ATGCGTGCGTAACGGATATTTCCGGGGTTCCTTTTACGTTCGAAACGATACGTCGGATTCGCCTGAGCGAAAAAGTGATG
GGCAATCTATTGTGTGTTACCCAGGCGGGCGGAGCCTTGACGCCGCGGCTGACACAGTATTTCTGGCAGTACTTCGCGGA
CCACAACGTGCGCTATTTCACGATGTACGGACAGACGGAGGCGGCGCCGCGCATTTCCTACGTGCCGCCGGATCGGGCCA
TGGACAAGCTGGGAAGCGTGGGCATCCCGATTCCCGGCGGAAAAATCGAGATCGTCGAAGAGGTTCCAGGGGCCGGGGAG
GGCGAGCTGGTCTATGAAGGCCCGAATGTTTCGATGGGCTATGCCAATTCCTGGCAAGACCTCGCCACGGGTGACCAGTT
CGGAGGGCGACTGCATACCGGTGATCAGGCCCGTATCGATGCGGACGGTTTCGTGACCATCGTCGGGCGCAGGAAGCGTT
TCATCAAATTGCACGGCATTTCCGTCAATCTGGATCACGTCGAATCCGTGCTCCGGAGCGCCGGGGTAAATTGCATTCTC
GTGGGAAAAGAAAACTGCGTGGTCATCTGTACCATGGCTGTCGACGTAAATGCCGTGCAGACGGCGCTGAAGGAGAATTT
CGATTTTCATCCCTCTGCGGTCCGCGTCGAAATTTGTGACGAACTGCCATTTACCGCGTCCAACAAGCCAGATTACGCCA
CGTTGACGCAGCGTTTTCTGACCTAG

Upstream 100 bases:

>100_bases
GAGACGTTCGACATCATGCTCGAAATGGATGATGTCGTCGATTTCGGCTCTTATGAGACCGGATTCGACACGCTCAAAAA
ATATGGCGTGACCTTCTGAG

Downstream 100 bases:

>100_bases
ATGTTCGGTCCCAAGGTTTGACCACGGAAAATCCAAGGCGCAGGTCGCGGACGGCACGACGCGAGACGGGATTTGGATCA
AGGAGACAAGGCGTTGAGTT

Product: 2-succinylbenzoate--CoA ligase

Products: NA

Alternate protein names: Long-chain acyl-CoA synthetase [H]

Number of amino acids: Translated: 461; Mature: 461

Protein sequence:

>461_residues
MFWQDRPSDAEDRTFLLEEGRRYSYHDIFRLGDALFADSSRAVAVILCDRTHETVAAYFGALRHGVVPLLLDGHARWPTL
SKTIEAYAPRYLVGNADAVPDGYIPTKRLVSKVLFERVVPIDYSLNENLALLIPTSGSTGDSKCVRLTGRNIESCTRAIV
SYLAITEERVTVSLLPFHYSYGLSVLHNTAFVRGTMLLTEQSVLEKNLWQRIEDACVTDISGVPFTFETIRRIRLSEKVM
GNLLCVTQAGGALTPRLTQYFWQYFADHNVRYFTMYGQTEAAPRISYVPPDRAMDKLGSVGIPIPGGKIEIVEEVPGAGE
GELVYEGPNVSMGYANSWQDLATGDQFGGRLHTGDQARIDADGFVTIVGRRKRFIKLHGISVNLDHVESVLRSAGVNCIL
VGKENCVVICTMAVDVNAVQTALKENFDFHPSAVRVEICDELPFTASNKPDYATLTQRFLT

Sequences:

>Translated_461_residues
MFWQDRPSDAEDRTFLLEEGRRYSYHDIFRLGDALFADSSRAVAVILCDRTHETVAAYFGALRHGVVPLLLDGHARWPTL
SKTIEAYAPRYLVGNADAVPDGYIPTKRLVSKVLFERVVPIDYSLNENLALLIPTSGSTGDSKCVRLTGRNIESCTRAIV
SYLAITEERVTVSLLPFHYSYGLSVLHNTAFVRGTMLLTEQSVLEKNLWQRIEDACVTDISGVPFTFETIRRIRLSEKVM
GNLLCVTQAGGALTPRLTQYFWQYFADHNVRYFTMYGQTEAAPRISYVPPDRAMDKLGSVGIPIPGGKIEIVEEVPGAGE
GELVYEGPNVSMGYANSWQDLATGDQFGGRLHTGDQARIDADGFVTIVGRRKRFIKLHGISVNLDHVESVLRSAGVNCIL
VGKENCVVICTMAVDVNAVQTALKENFDFHPSAVRVEICDELPFTASNKPDYATLTQRFLT
>Mature_461_residues
MFWQDRPSDAEDRTFLLEEGRRYSYHDIFRLGDALFADSSRAVAVILCDRTHETVAAYFGALRHGVVPLLLDGHARWPTL
SKTIEAYAPRYLVGNADAVPDGYIPTKRLVSKVLFERVVPIDYSLNENLALLIPTSGSTGDSKCVRLTGRNIESCTRAIV
SYLAITEERVTVSLLPFHYSYGLSVLHNTAFVRGTMLLTEQSVLEKNLWQRIEDACVTDISGVPFTFETIRRIRLSEKVM
GNLLCVTQAGGALTPRLTQYFWQYFADHNVRYFTMYGQTEAAPRISYVPPDRAMDKLGSVGIPIPGGKIEIVEEVPGAGE
GELVYEGPNVSMGYANSWQDLATGDQFGGRLHTGDQARIDADGFVTIVGRRKRFIKLHGISVNLDHVESVLRSAGVNCIL
VGKENCVVICTMAVDVNAVQTALKENFDFHPSAVRVEICDELPFTASNKPDYATLTQRFLT

Specific function: Involved in the degradation of long-chain fatty acids [H]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Partially Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1788107, Length=276, Percent_Identity=29.3478260869565, Blast_Score=93, Evalue=4e-20,
Organism=Escherichia coli, GI1789201, Length=357, Percent_Identity=28.8515406162465, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI145693145, Length=278, Percent_Identity=26.6187050359712, Blast_Score=79, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI17559526, Length=360, Percent_Identity=26.6666666666667, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI18859661, Length=311, Percent_Identity=26.6881028938907, Blast_Score=78, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020459
- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.3 [H]

Molecular weight: Translated: 51215; Mature: 51215

Theoretical pI: Translated: 6.14; Mature: 6.14

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFWQDRPSDAEDRTFLLEEGRRYSYHDIFRLGDALFADSSRAVAVILCDRTHETVAAYFG
CCCCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHCCCCCCEEEEEEECCCHHHHHHHHH
ALRHGVVPLLLDGHARWPTLSKTIEAYAPRYLVGNADAVPDGYIPTKRLVSKVLFERVVP
HHHHCCEEEEECCCCCCCHHHHHHHHHCCEEEECCCCCCCCCCCCHHHHHHHHHHHHCCC
IDYSLNENLALLIPTSGSTGDSKCVRLTGRNIESCTRAIVSYLAITEERVTVSLLPFHYS
CCEECCCCEEEEEECCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHCCCEEEEEEEEEHH
YGLSVLHNTAFVRGTMLLTEQSVLEKNLWQRIEDACVTDISGVPFTFETIRRIRLSEKVM
CCHHHHHHHHHHEEEEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHH
GNLLCVTQAGGALTPRLTQYFWQYFADHNVRYFTMYGQTEAAPRISYVPPDRAMDKLGSV
CCEEEEECCCCCCCHHHHHHHHHHHHCCCEEEEEEECCCCCCCEEECCCCHHHHHHHCCC
GIPIPGGKIEIVEEVPGAGEGELVYEGPNVSMGYANSWQDLATGDQFGGRLHTGDQARID
CCCCCCCCEEEEECCCCCCCCCEEEECCCCEECCCCCCHHHCCCHHCCCEEECCCCCEEC
ADGFVTIVGRRKRFIKLHGISVNLDHVESVLRSAGVNCILVGKENCVVICTMAVDVNAVQ
CCCEEEEEECCEEEEEEEEEEEEHHHHHHHHHHCCCCEEEECCCCEEEEEEEEECHHHHH
TALKENFDFHPSAVRVEICDELPFTASNKPDYATLTQRFLT
HHHHHCCCCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHCC
>Mature Secondary Structure
MFWQDRPSDAEDRTFLLEEGRRYSYHDIFRLGDALFADSSRAVAVILCDRTHETVAAYFG
CCCCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHCCCCCCEEEEEEECCCHHHHHHHHH
ALRHGVVPLLLDGHARWPTLSKTIEAYAPRYLVGNADAVPDGYIPTKRLVSKVLFERVVP
HHHHCCEEEEECCCCCCCHHHHHHHHHCCEEEECCCCCCCCCCCCHHHHHHHHHHHHCCC
IDYSLNENLALLIPTSGSTGDSKCVRLTGRNIESCTRAIVSYLAITEERVTVSLLPFHYS
CCEECCCCEEEEEECCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHCCCEEEEEEEEEHH
YGLSVLHNTAFVRGTMLLTEQSVLEKNLWQRIEDACVTDISGVPFTFETIRRIRLSEKVM
CCHHHHHHHHHHEEEEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHH
GNLLCVTQAGGALTPRLTQYFWQYFADHNVRYFTMYGQTEAAPRISYVPPDRAMDKLGSV
CCEEEEECCCCCCCHHHHHHHHHHHHCCCEEEEEEECCCCCCCEEECCCCHHHHHHHCCC
GIPIPGGKIEIVEEVPGAGEGELVYEGPNVSMGYANSWQDLATGDQFGGRLHTGDQARID
CCCCCCCCEEEEECCCCCCCCCEEEECCCCEECCCCCCHHHCCCHHCCCEEECCCCCEEC
ADGFVTIVGRRKRFIKLHGISVNLDHVESVLRSAGVNCILVGKENCVVICTMAVDVNAVQ
CCCEEEEEECCEEEEEEEEEEEEHHHHHHHHHHCCCCEEEECCCCEEEEEEEEECHHHHH
TALKENFDFHPSAVRVEICDELPFTASNKPDYATLTQRFLT
HHHHHCCCCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377 [H]