Click here to switch to the map view.

The map label for this gene is mycA [H]

Identifier: 162148697

GI number: 162148697

Start: 3014939

End: 3016675

Strand: Direct

Name: mycA [H]

Synonym: GDI_2925

Alternate gene names: 162148697

Gene position: 3014939-3016675 (Clockwise)

Preceding gene: 162148696

Following gene: 162148698

Centisome position: 76.44

GC content: 68.45

Gene sequence:

>1737_bases
ATGACACGTGATCCGATTCCGACAGATTCTGGTCAGGCGCGACGCCTGGGCGATTTTGCAACGTTTCCGGAAGCGCTGGA
TTACGCGGCCCTCGGTGACGCCGGCTTCAACATCTATTCCGGCCGTGGCCAGCTTCTGGAAGCCCTGCCCTACCGGACCC
TGCGCGCGCAGGCGGTGGAGGTGGCGCGCCGCCTGCTGACGCTGGGGCTGCGCCCGGGCGAGCGGGTCGCGGTCGTGGCG
GAGAGCGACGGCGACTTCGCCCGCATCTTCTTCGGGTGCCAGTATGCCGGCCTGGTGCCGGCGCCGCTGCCGCTGCCGGT
CGCATTCGGCGGCCGCGAGGGCTATGTCGCCACCCTGCGCGGCATGATCCAGAGCGCCGGCGCCGCCGCCGTCATCGTGC
CCGATATCATCGCGGGCTGGACCGACGACATCGTGGGCGGTTTCGATCTGCGCTTCGCGGGGCGTCCGGCGGATTTGATG
GCGCTGCCGGCGGATACGATCGACCTGCCCGTCGTGTCCCCCGACGACCTGTCCTACCTGCAGTTCTCATCGGGCAGCAC
CCGCTTCCCGATGGGCGTCGCCGTCACCCAGCGGTCGGGCATGGCCAATGCCCGCGCCATCGCCCGCGACGGGTTGAAGG
TGCGCGAGACCGGGGACCGCTGCGTCTCGTGGCTGCCGCTGTATCACGACATGGGGCTGGTCGGCTTCTTCCTGACCCCG
ATGACCTGCCAGTTGACCGTGGATATGCTGCCGACGCGGGAATTCGCGCGGCGCCCGCATGTCTGGCTGGACCTGATCAG
CCGCAATCGCGCCACCATTTCCTACAGCCCGTCCTTCGGCTTCGAACTGTGCGCCCGGCGGGCCACGTCGATCGCGCTGG
ACCTGTCCTGCTGGCGGATCGCGGGGATCGGCGGCGACATGATCCGCCACCACATCCTGGAAGAATTCGCCGAGCGATTC
GCGGGCGCCGGGTTCGACGGGCGGGCCTTCACCGCCAGCTACGGGATGGCCGAGGCGACGCTGGCCATCAGCTTCGTTCC
CCTGCAGACCGGAATCCAGACTGATACGGTCGACCTGCGCGTGCTGGAAACCCAGGGCGTGGCACGCCCTTCGGACGATC
CGTCGCATGCCCTGCGGACGTTCGTGCTGTGCGGGCAGGTCCTGGACGAGCACGAACTGGAGGTTCGCGACCCGGCGGGC
CGGCCGCTGCCCGACCGCAAGGTCGGCACCATCTTCGTGCGCGGGCCCAGCCTGATGCGCGGCTATTTCAACCAGCCGCA
GGAGACCCAGCGGGTTCTGGACAGCGCGGGATGGCTGAACACCGGCGACCTTGGCTACATGCTCGATGGGCAGGTGGTCG
TGACCGGACGGGCCAAGGACCTGATCATCATCAACGGCCGCAACATCTGGCCGCAGGACCTGGAATGGTCGGCCGAGACC
GAGATCGGGTCCCTGCGCAGCCGGGACGTCGCGGTGTTTTCGATCGACCAGGAAACGGGCGAGACGGTGGTGGCGCTGAT
TCAGTGCCGGGCGTCCACCCCCGAGGCACGTGAACTGCTGCGCAGCGAGGCCGCGAGCCTGTTCCGCCGGCAGCACGGCG
TCGATGTCTCGGTCATCCTGGTGCCGCCGCACACGCTGCCCCAGACATCTTCGGGAAAACTGACGCGCGCGCGCGCCAAG
GCCATGCTGCTGTCCGGCGCGTTCGAAACCATTACCGAAACCGCATCCGTCGCCTGA

Upstream 100 bases:

>100_bases
CGATCGTAGAATTTCTCCGGAAGGCGGGGTAATGAGCGCCCTTGCCGCGACATCAGGCCCGCATCATTACACGCTCCAGG
TGGAAGATTCGTTACCAGAC

Downstream 100 bases:

>100_bases
AGCGGCGCCCCGTCGTACCCGCAACGATGCCCCGCGCTTCGATGCTGGCCAAGGCACCGGATTTCGATCATAAGGACGTC
CTCTTTCCACGGAGGGCGGC

Product: acyl-CoA synthetase

Products: AMP; diphosphate +an acyl-CoA [C]

Alternate protein names: Glutamate-1-semialdehyde aminotransferase; GSA-AT; ATP-dependent asparagine adenylase 1; AsnA 1; Asparagine activase 1 [H]

Number of amino acids: Translated: 578; Mature: 577

Protein sequence:

>578_residues
MTRDPIPTDSGQARRLGDFATFPEALDYAALGDAGFNIYSGRGQLLEALPYRTLRAQAVEVARRLLTLGLRPGERVAVVA
ESDGDFARIFFGCQYAGLVPAPLPLPVAFGGREGYVATLRGMIQSAGAAAVIVPDIIAGWTDDIVGGFDLRFAGRPADLM
ALPADTIDLPVVSPDDLSYLQFSSGSTRFPMGVAVTQRSGMANARAIARDGLKVRETGDRCVSWLPLYHDMGLVGFFLTP
MTCQLTVDMLPTREFARRPHVWLDLISRNRATISYSPSFGFELCARRATSIALDLSCWRIAGIGGDMIRHHILEEFAERF
AGAGFDGRAFTASYGMAEATLAISFVPLQTGIQTDTVDLRVLETQGVARPSDDPSHALRTFVLCGQVLDEHELEVRDPAG
RPLPDRKVGTIFVRGPSLMRGYFNQPQETQRVLDSAGWLNTGDLGYMLDGQVVVTGRAKDLIIINGRNIWPQDLEWSAET
EIGSLRSRDVAVFSIDQETGETVVALIQCRASTPEARELLRSEAASLFRRQHGVDVSVILVPPHTLPQTSSGKLTRARAK
AMLLSGAFETITETASVA

Sequences:

>Translated_578_residues
MTRDPIPTDSGQARRLGDFATFPEALDYAALGDAGFNIYSGRGQLLEALPYRTLRAQAVEVARRLLTLGLRPGERVAVVA
ESDGDFARIFFGCQYAGLVPAPLPLPVAFGGREGYVATLRGMIQSAGAAAVIVPDIIAGWTDDIVGGFDLRFAGRPADLM
ALPADTIDLPVVSPDDLSYLQFSSGSTRFPMGVAVTQRSGMANARAIARDGLKVRETGDRCVSWLPLYHDMGLVGFFLTP
MTCQLTVDMLPTREFARRPHVWLDLISRNRATISYSPSFGFELCARRATSIALDLSCWRIAGIGGDMIRHHILEEFAERF
AGAGFDGRAFTASYGMAEATLAISFVPLQTGIQTDTVDLRVLETQGVARPSDDPSHALRTFVLCGQVLDEHELEVRDPAG
RPLPDRKVGTIFVRGPSLMRGYFNQPQETQRVLDSAGWLNTGDLGYMLDGQVVVTGRAKDLIIINGRNIWPQDLEWSAET
EIGSLRSRDVAVFSIDQETGETVVALIQCRASTPEARELLRSEAASLFRRQHGVDVSVILVPPHTLPQTSSGKLTRARAK
AMLLSGAFETITETASVA
>Mature_577_residues
TRDPIPTDSGQARRLGDFATFPEALDYAALGDAGFNIYSGRGQLLEALPYRTLRAQAVEVARRLLTLGLRPGERVAVVAE
SDGDFARIFFGCQYAGLVPAPLPLPVAFGGREGYVATLRGMIQSAGAAAVIVPDIIAGWTDDIVGGFDLRFAGRPADLMA
LPADTIDLPVVSPDDLSYLQFSSGSTRFPMGVAVTQRSGMANARAIARDGLKVRETGDRCVSWLPLYHDMGLVGFFLTPM
TCQLTVDMLPTREFARRPHVWLDLISRNRATISYSPSFGFELCARRATSIALDLSCWRIAGIGGDMIRHHILEEFAERFA
GAGFDGRAFTASYGMAEATLAISFVPLQTGIQTDTVDLRVLETQGVARPSDDPSHALRTFVLCGQVLDEHELEVRDPAGR
PLPDRKVGTIFVRGPSLMRGYFNQPQETQRVLDSAGWLNTGDLGYMLDGQVVVTGRAKDLIIINGRNIWPQDLEWSAETE
IGSLRSRDVAVFSIDQETGETVVALIQCRASTPEARELLRSEAASLFRRQHGVDVSVILVPPHTLPQTSSGKLTRARAKA
MLLSGAFETITETASVA

Specific function: This protein is a multifunctional enzyme, able to activate a long chain fatty acid and link it with the amino acid Asn as part of the synthesis of mycosubtilin. The activation sites consist of individual domains [H]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Partially Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 4 acyl carrier domains [H]

Homologues:

Organism=Homo sapiens, GI44888818, Length=449, Percent_Identity=25.6124721603563, Blast_Score=82, Evalue=1e-15,
Organism=Homo sapiens, GI187761345, Length=396, Percent_Identity=24.7474747474747, Blast_Score=80, Evalue=8e-15,
Organism=Homo sapiens, GI187761343, Length=396, Percent_Identity=24.7474747474747, Blast_Score=80, Evalue=8e-15,
Organism=Homo sapiens, GI45827692, Length=519, Percent_Identity=22.7360308285164, Blast_Score=76, Evalue=1e-13,
Organism=Homo sapiens, GI225735629, Length=519, Percent_Identity=22.7360308285164, Blast_Score=75, Evalue=1e-13,
Organism=Escherichia coli, GI1788107, Length=557, Percent_Identity=22.8007181328546, Blast_Score=99, Evalue=6e-22,
Organism=Escherichia coli, GI145693145, Length=533, Percent_Identity=26.078799249531, Blast_Score=94, Evalue=3e-20,
Organism=Escherichia coli, GI1786810, Length=548, Percent_Identity=22.4452554744526, Blast_Score=75, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI71994703, Length=563, Percent_Identity=22.9129662522202, Blast_Score=83, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI71994694, Length=563, Percent_Identity=22.9129662522202, Blast_Score=83, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI71994690, Length=563, Percent_Identity=22.9129662522202, Blast_Score=82, Evalue=7e-16,
Organism=Caenorhabditis elegans, GI17557194, Length=432, Percent_Identity=24.537037037037, Blast_Score=82, Evalue=9e-16,
Organism=Caenorhabditis elegans, GI17559526, Length=550, Percent_Identity=22.9090909090909, Blast_Score=81, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17560308, Length=548, Percent_Identity=22.4452554744526, Blast_Score=81, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17531443, Length=415, Percent_Identity=23.855421686747, Blast_Score=81, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17538037, Length=425, Percent_Identity=24.7058823529412, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24581924, Length=389, Percent_Identity=22.107969151671, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI18859661, Length=412, Percent_Identity=23.3009708737864, Blast_Score=82, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24653035, Length=179, Percent_Identity=30.7262569832402, Blast_Score=77, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24654656, Length=649, Percent_Identity=23.1124807395994, Blast_Score=77, Evalue=5e-14,
Organism=Drosophila melanogaster, GI24648260, Length=203, Percent_Identity=26.1083743842365, Blast_Score=70, Evalue=6e-12,
Organism=Drosophila melanogaster, GI161076582, Length=177, Percent_Identity=28.8135593220339, Blast_Score=70, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010071
- InterPro:   IPR009081
- InterPro:   IPR005814
- InterPro:   IPR020845
- InterPro:   IPR000873
- InterPro:   IPR023213
- InterPro:   IPR001242
- InterPro:   IPR018201
- InterPro:   IPR014031
- InterPro:   IPR014030
- InterPro:   IPR006163
- InterPro:   IPR020806
- InterPro:   IPR006162
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- InterPro:   IPR016039
- InterPro:   IPR016038 [H]

Pfam domain/function: PF00202 Aminotran_3; PF00501 AMP-binding; PF00668 Condensation; PF00109 ketoacyl-synt; PF02801 Ketoacyl-synt_C; PF00550 PP-binding [H]

EC number: 6.2.1.3 [C]

Molecular weight: Translated: 62802; Mature: 62671

Theoretical pI: Translated: 5.39; Mature: 5.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRDPIPTDSGQARRLGDFATFPEALDYAALGDAGFNIYSGRGQLLEALPYRTLRAQAVE
CCCCCCCCCCCCCHHCCCHHHCHHHHHHHHCCCCCCEEECCCHHHHHHCCCHHHHHHHHH
VARRLLTLGLRPGERVAVVAESDGDFARIFFGCQYAGLVPAPLPLPVAFGGREGYVATLR
HHHHHHHHCCCCCCEEEEEEECCCCEEEEEEECEECCCCCCCCCCEEEECCCCCHHHHHH
GMIQSAGAAAVIVPDIIAGWTDDIVGGFDLRFAGRPADLMALPADTIDLPVVSPDDLSYL
HHHHCCCCEEEEECHHHCCCCHHHCCCEEEEECCCCCCEEEECCCCCCCCCCCCCCCCEE
QFSSGSTRFPMGVAVTQRSGMANARAIARDGLKVRETGDRCVSWLPLYHDMGLVGFFLTP
EECCCCCCCCCEEEEECCCCCCHHHHHHHCCCEEECCHHHHHHHHHHHHHHHHHHHHHCC
MTCQLTVDMLPTREFARRPHVWLDLISRNRATISYSPSFGFELCARRATSIALDLSCWRI
EEEEEEEECCCCHHHHHCCCHHHHHHCCCCEEEEECCCCCHHHHHHHHCEEEEEEEEEEE
AGIGGDMIRHHILEEFAERFAGAGFDGRAFTASYGMAEATLAISFVPLQTGIQTDTVDLR
ECCCHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCEEEEEEEEEEECCCCCCCCEEEE
VLETQGVARPSDDPSHALRTFVLCGQVLDEHELEVRDPAGRPLPDRKVGTIFVRGPSLMR
EEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCEEEEEEECHHHHH
GYFNQPQETQRVLDSAGWLNTGDLGYMLDGQVVVTGRAKDLIIINGRNIWPQDLEWSAET
HHCCCCHHHHHHHHHCCCCCCCCCCEEECCEEEEECCCCEEEEECCCCCCCCCCCCCCCC
EIGSLRSRDVAVFSIDQETGETVVALIQCRASTPEARELLRSEAASLFRRQHGVDVSVIL
CCCCCCCCCEEEEEECCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCEEEEE
VPPHTLPQTSSGKLTRARAKAMLLSGAFETITETASVA
ECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TRDPIPTDSGQARRLGDFATFPEALDYAALGDAGFNIYSGRGQLLEALPYRTLRAQAVE
CCCCCCCCCCCCHHCCCHHHCHHHHHHHHCCCCCCEEECCCHHHHHHCCCHHHHHHHHH
VARRLLTLGLRPGERVAVVAESDGDFARIFFGCQYAGLVPAPLPLPVAFGGREGYVATLR
HHHHHHHHCCCCCCEEEEEEECCCCEEEEEEECEECCCCCCCCCCEEEECCCCCHHHHHH
GMIQSAGAAAVIVPDIIAGWTDDIVGGFDLRFAGRPADLMALPADTIDLPVVSPDDLSYL
HHHHCCCCEEEEECHHHCCCCHHHCCCEEEEECCCCCCEEEECCCCCCCCCCCCCCCCEE
QFSSGSTRFPMGVAVTQRSGMANARAIARDGLKVRETGDRCVSWLPLYHDMGLVGFFLTP
EECCCCCCCCCEEEEECCCCCCHHHHHHHCCCEEECCHHHHHHHHHHHHHHHHHHHHHCC
MTCQLTVDMLPTREFARRPHVWLDLISRNRATISYSPSFGFELCARRATSIALDLSCWRI
EEEEEEEECCCCHHHHHCCCHHHHHHCCCCEEEEECCCCCHHHHHHHHCEEEEEEEEEEE
AGIGGDMIRHHILEEFAERFAGAGFDGRAFTASYGMAEATLAISFVPLQTGIQTDTVDLR
ECCCHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCEEEEEEEEEEECCCCCCCCEEEE
VLETQGVARPSDDPSHALRTFVLCGQVLDEHELEVRDPAGRPLPDRKVGTIFVRGPSLMR
EEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCEEEEEEECHHHHH
GYFNQPQETQRVLDSAGWLNTGDLGYMLDGQVVVTGRAKDLIIINGRNIWPQDLEWSAET
HHCCCCHHHHHHHHHCCCCCCCCCCEEECCEEEEECCCCEEEEECCCCCCCCCCCCCCCC
EIGSLRSRDVAVFSIDQETGETVVALIQCRASTPEARELLRSEAASLFRRQHGVDVSVIL
CCCCCCCCCEEEEEECCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCEEEEE
VPPHTLPQTSSGKLTRARAKAMLLSGAFETITETASVA
ECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Salts [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0024 {myristate}} 0.0021 {myristate}} 0.0012 {myristate}} 0.0002 {myristate}} 0.0408 {octanoate}} 0.0125 {octanoate}} 0.0059 {octanoate}} 0.00072 {octanoate}} 0.0112 {hexanoate}} 0.0083 {decanoate}} 0.0063 {decanoate}} 0.0043 {dec

Substrates: ATP; a long-chain carboxylic acid; CoA [C]

Specific reaction: ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate +an acyl-CoA [C]

General reaction: Acid-thiol ligation; Phosphorylation [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10557314 [H]