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The map label for this gene is thiF [C]

Identifier: 162148664

GI number: 162148664

Start: 2977237

End: 2978994

Strand: Reverse

Name: thiF [C]

Synonym: GDI_2891

Alternate gene names: 162148664

Gene position: 2978994-2977237 (Counterclockwise)

Preceding gene: 162148665

Following gene: 162148661

Centisome position: 75.53

GC content: 65.76

Gene sequence:

>1758_bases
GTGCCCGAGCGCCTCGACCACGCGACGATCTCCAGCGACTATCCCGGCCGTGACTGGATCGTCGCCGCCTGGCGGATCAG
CGTGGTGTTTTCCGATGGAGTAACCCGCCGAATCGACCTTGTGGCCGGCGCGCAGTTTCCGACGACGCCGATCAGGACCG
CGCTGGTCGACCATCCCGAGTCCATGACCTGGCCGCACGTCGAAAGCGACGGCATCCTGTGCCTATTGCCGAACATGGCC
GAGTGGGATCCCGATGATCCTTCAGAGGTCGCGATGGATCTACTAAATCGCTCGGTCCGGCTGGTCGAGGAGTTACTTGA
GGGCAGCATCGTCGAGCGAGACTTCCGCGACGAGTTTGTCACCTACTGGGGTTACCGGGTGCATTCGCAGGGCGAAAACC
TGTTCAGCCTGGTCGCGCCCGAGCCGCCCTCGCGGGTGGTGCGTGTGTGGCGCGGCAAGAATCTCGAAGTCGCCGGAGAG
GACCAGGACGCGATTGCGAAATGGGTGTGTCACCGCTTTGGCGACAAGACTCCGGTGAAGACGGAAGCGGCCGCGTTCCT
CTGGCTCGACGAACCACTGCTTCCTGCAGAATACCCAGAGACGGCCGCCGATCTGTATCATTTGGGGGAGCGGATCGCTG
GCGAGGCGAGGACGGTCTTGGAGCAGGCGGCGAAAGGCGAGCCGGATGAGGTGCTGGTGCTGCTCGGCGCTATTGGACGC
GGGGGCTCCGCATCGATTGGGGTGAAGGCGCTCAACCCAAAGCGACGCAAAGGCCGGCCGCGCACTCCCGACGAACCCCT
CTCCGTGGGATTCCGACCCGGCCATACCCCACAGCCGCTGCTGCTCGACCGCTTTTTCGGCACGGCTCCGGTCGTCAGGA
CGCTAGTGCAGCGGGCCGATGCCGATTGGGTGCATGGGCGCGGTCAGGATGCCCGCACGCGCCGGCTGCTCGGCGCGACG
GTCGTGGTGATCGGCTGCGGTTCGGTCGGGGCACCGGTCGCCTGCGCGCTGGCCCAAGCGGGTGTCGGCCGCCTCATCCT
AGTCGATCCAGAACGGTTGACCTGGCCCAACGTCGGGCGCCATCCGCTCGGCGCCACCGCCGTTGGGCGCAACAAGGCCG
AAGCCTTGGCCGAACGACTGCAGGTCGACTTTCCGCATCTGAATGTCGAACATCGCGCTTTCGGCCTGCATCAGCTGATC
GCGCGCAACGAGGATCTTCTCGCAGCGACCGACATGATCGTGGCTGCGACCGGAAATTGGGTCGCCGAGAGTGCGCTCAA
TCGCTGGCATGTCCGGCAAGGGCGCGCACGGCCCGTTGTCTACGGGTGGACCGAGGCGCATGCCTGCGCTGGCCACGCCG
TCGCCATCGGCCAGAGGGGGGGCTGCCTGCAATGTCACATCGGGCGCACCGGCGCGCCCGAACTCATGGCTGTGGAGTGG
CCGGATGGCGGCGACATCGCTCAGGAGGAGCCGGCCTGCGGCGCGCACTATCAGCCCTATGGGCCGGTGGAGCTCGCTTA
CGTGAACGCGATGATCACAGAAGTCGCGCTGGACTGTCTTCTCGATCCACCGGCGCAATCATTCAGTCGCGTATTCGTCG
CCGCGCCGCGCCGCATCGCGTCACTTGGCGGGCAGCTGACCGAACACTGGCGCTCCGCTTTTGGTGAAGTTGAAGAGGGC
GTTCGGACGGTCGACCGACCTTGGCCTTCTGCTAATTGCGCAGCTTGCGGGCCTGGGCATCTCGACGAAGCTGCCTAA

Upstream 100 bases:

>100_bases
AGCGCCGGCGGCGGCCGCCATGCTTAAGGACGGCCTCGCGCTCGATTGGCGACGGGCAGCGCTCCTACTCGAGGCGGCCC
TGCGCGAGCGGGCGGAGCAA

Downstream 100 bases:

>100_bases
TGCTGCCCCAAGATCGAGCTTCTCAGACCTCAGCCGACCAGCGACAGCGTTAAGCTGGGTCCGCTTCAAGCGCTAAACAG
GCTTTGCAGCGATATCTTAC

Product: ubiquitin-activating enzyme

Products: ThiS-COSH; AMP; ThiS-COAMP; pyrophosphate [C]

Alternate protein names: Ubiquitin-Activating; Molybdopterin Biosynthesis Protein; Dinucleotide-Utilizing S

Number of amino acids: Translated: 585; Mature: 584

Protein sequence:

>585_residues
MPERLDHATISSDYPGRDWIVAAWRISVVFSDGVTRRIDLVAGAQFPTTPIRTALVDHPESMTWPHVESDGILCLLPNMA
EWDPDDPSEVAMDLLNRSVRLVEELLEGSIVERDFRDEFVTYWGYRVHSQGENLFSLVAPEPPSRVVRVWRGKNLEVAGE
DQDAIAKWVCHRFGDKTPVKTEAAAFLWLDEPLLPAEYPETAADLYHLGERIAGEARTVLEQAAKGEPDEVLVLLGAIGR
GGSASIGVKALNPKRRKGRPRTPDEPLSVGFRPGHTPQPLLLDRFFGTAPVVRTLVQRADADWVHGRGQDARTRRLLGAT
VVVIGCGSVGAPVACALAQAGVGRLILVDPERLTWPNVGRHPLGATAVGRNKAEALAERLQVDFPHLNVEHRAFGLHQLI
ARNEDLLAATDMIVAATGNWVAESALNRWHVRQGRARPVVYGWTEAHACAGHAVAIGQRGGCLQCHIGRTGAPELMAVEW
PDGGDIAQEEPACGAHYQPYGPVELAYVNAMITEVALDCLLDPPAQSFSRVFVAAPRRIASLGGQLTEHWRSAFGEVEEG
VRTVDRPWPSANCAACGPGHLDEAA

Sequences:

>Translated_585_residues
MPERLDHATISSDYPGRDWIVAAWRISVVFSDGVTRRIDLVAGAQFPTTPIRTALVDHPESMTWPHVESDGILCLLPNMA
EWDPDDPSEVAMDLLNRSVRLVEELLEGSIVERDFRDEFVTYWGYRVHSQGENLFSLVAPEPPSRVVRVWRGKNLEVAGE
DQDAIAKWVCHRFGDKTPVKTEAAAFLWLDEPLLPAEYPETAADLYHLGERIAGEARTVLEQAAKGEPDEVLVLLGAIGR
GGSASIGVKALNPKRRKGRPRTPDEPLSVGFRPGHTPQPLLLDRFFGTAPVVRTLVQRADADWVHGRGQDARTRRLLGAT
VVVIGCGSVGAPVACALAQAGVGRLILVDPERLTWPNVGRHPLGATAVGRNKAEALAERLQVDFPHLNVEHRAFGLHQLI
ARNEDLLAATDMIVAATGNWVAESALNRWHVRQGRARPVVYGWTEAHACAGHAVAIGQRGGCLQCHIGRTGAPELMAVEW
PDGGDIAQEEPACGAHYQPYGPVELAYVNAMITEVALDCLLDPPAQSFSRVFVAAPRRIASLGGQLTEHWRSAFGEVEEG
VRTVDRPWPSANCAACGPGHLDEAA
>Mature_584_residues
PERLDHATISSDYPGRDWIVAAWRISVVFSDGVTRRIDLVAGAQFPTTPIRTALVDHPESMTWPHVESDGILCLLPNMAE
WDPDDPSEVAMDLLNRSVRLVEELLEGSIVERDFRDEFVTYWGYRVHSQGENLFSLVAPEPPSRVVRVWRGKNLEVAGED
QDAIAKWVCHRFGDKTPVKTEAAAFLWLDEPLLPAEYPETAADLYHLGERIAGEARTVLEQAAKGEPDEVLVLLGAIGRG
GSASIGVKALNPKRRKGRPRTPDEPLSVGFRPGHTPQPLLLDRFFGTAPVVRTLVQRADADWVHGRGQDARTRRLLGATV
VVIGCGSVGAPVACALAQAGVGRLILVDPERLTWPNVGRHPLGATAVGRNKAEALAERLQVDFPHLNVEHRAFGLHQLIA
RNEDLLAATDMIVAATGNWVAESALNRWHVRQGRARPVVYGWTEAHACAGHAVAIGQRGGCLQCHIGRTGAPELMAVEWP
DGGDIAQEEPACGAHYQPYGPVELAYVNAMITEVALDCLLDPPAQSFSRVFVAAPRRIASLGGQLTEHWRSAFGEVEEGV
RTVDRPWPSANCAACGPGHLDEAA

Specific function: Catalyzes The Adenylation By ATP Of The Carboxyl- Terminal Glycine Of This. [C]

COG id: COG0476

COG function: function code H; Dinucleotide-utilizing enzymes involved in molybdopterin and thiamine biosynthesis family 2

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 2.7.7.- [C]

Molecular weight: Translated: 63728; Mature: 63597

Theoretical pI: Translated: 5.69; Mature: 5.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPERLDHATISSDYPGRDWIVAAWRISVVFSDGVTRRIDLVAGAQFPTTPIRTALVDHPE
CCCCCCCCCCCCCCCCCCEEEEEEEEEEEECCCCCEEEEEEECCCCCCCHHHHHHHCCCC
SMTWPHVESDGILCLLPNMAEWDPDDPSEVAMDLLNRSVRLVEELLEGSIVERDFRDEFV
CCCCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
TYWGYRVHSQGENLFSLVAPEPPSRVVRVWRGKNLEVAGEDQDAIAKWVCHRFGDKTPVK
HHHCEEEECCCCCEEEEECCCCHHHHHHHHCCCCEEECCCCHHHHHHHHHHHCCCCCCCC
TEAAAFLWLDEPLLPAEYPETAADLYHLGERIAGEARTVLEQAAKGEPDEVLVLLGAIGR
CCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCC
GGSASIGVKALNPKRRKGRPRTPDEPLSVGFRPGHTPQPLLLDRFFGTAPVVRTLVQRAD
CCCCCEEEEECCCHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCHHHHHHHHHHCC
ADWVHGRGQDARTRRLLGATVVVIGCGSVGAPVACALAQAGVGRLILVDPERLTWPNVGR
CCCCCCCCCCHHHHHHHCEEEEEEECCCCCHHHHHHHHHCCCCEEEEECCCCCCCCCCCC
HPLGATAVGRNKAEALAERLQVDFPHLNVEHRAFGLHQLIARNEDLLAATDMIVAATGNW
CCCCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCEEECCEEEEECCCH
VAESALNRWHVRQGRARPVVYGWTEAHACAGHAVAIGQRGGCLQCHIGRTGAPELMAVEW
HHHHHHHHHHHHCCCCCCEEEECCHHHHCCCCEEEECCCCCEEEEECCCCCCCCEEEEEC
PDGGDIAQEEPACGAHYQPYGPVELAYVNAMITEVALDCLLDPPAQSFSRVFVAAPRRIA
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCHHHHH
SLGGQLTEHWRSAFGEVEEGVRTVDRPWPSANCAACGPGHLDEAA
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
PERLDHATISSDYPGRDWIVAAWRISVVFSDGVTRRIDLVAGAQFPTTPIRTALVDHPE
CCCCCCCCCCCCCCCCCEEEEEEEEEEEECCCCCEEEEEEECCCCCCCHHHHHHHCCCC
SMTWPHVESDGILCLLPNMAEWDPDDPSEVAMDLLNRSVRLVEELLEGSIVERDFRDEFV
CCCCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
TYWGYRVHSQGENLFSLVAPEPPSRVVRVWRGKNLEVAGEDQDAIAKWVCHRFGDKTPVK
HHHCEEEECCCCCEEEEECCCCHHHHHHHHCCCCEEECCCCHHHHHHHHHHHCCCCCCCC
TEAAAFLWLDEPLLPAEYPETAADLYHLGERIAGEARTVLEQAAKGEPDEVLVLLGAIGR
CCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCC
GGSASIGVKALNPKRRKGRPRTPDEPLSVGFRPGHTPQPLLLDRFFGTAPVVRTLVQRAD
CCCCCEEEEECCCHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCHHHHHHHHHHCC
ADWVHGRGQDARTRRLLGATVVVIGCGSVGAPVACALAQAGVGRLILVDPERLTWPNVGR
CCCCCCCCCCHHHHHHHCEEEEEEECCCCCHHHHHHHHHCCCCEEEEECCCCCCCCCCCC
HPLGATAVGRNKAEALAERLQVDFPHLNVEHRAFGLHQLIARNEDLLAATDMIVAATGNW
CCCCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCEEECCEEEEECCCH
VAESALNRWHVRQGRARPVVYGWTEAHACAGHAVAIGQRGGCLQCHIGRTGAPELMAVEW
HHHHHHHHHHHHCCCCCCEEEECCHHHHCCCCEEEECCCCCEEEEECCCCCCCCEEEEEC
PDGGDIAQEEPACGAHYQPYGPVELAYVNAMITEVALDCLLDPPAQSFSRVFVAAPRRIA
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCHHHHH
SLGGQLTEHWRSAFGEVEEGVRTVDRPWPSANCAACGPGHLDEAA
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ThiS-COAMP; L-cysteine; ThiS protein; ATP [C]

Specific reaction: ThiS-COAMP + L-cysteine = ThiS-COSH + AMP ThiS protein + ATP = ThiS-COAMP + pyrophosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA