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The map label for this gene is gloA [H]

Identifier: 162148628

GI number: 162148628

Start: 2945152

End: 2945589

Strand: Reverse

Name: gloA [H]

Synonym: GDI_2855

Alternate gene names: 162148628

Gene position: 2945589-2945152 (Counterclockwise)

Preceding gene: 162148629

Following gene: 162148627

Centisome position: 74.68

GC content: 57.99

Gene sequence:

>438_bases
ATGCGTTTCCTTCATACCATGGTGCGAGTGTCCGACATTGAGGACTCACTGGCCTTTTATTGCGGCGCGCTCGGCCTCAA
GGAGGTAAGGCGTACCGAGAACGCGACCGGGAAGTTTACGCTCATATTCCTCGCGGCGCCCGGAGATGCCGAGGCTGCTG
ATAAACACGCCCCATTGGTCGAACTGACCCATAACTGGAACCAGGAGATATACACCGGCGGTCGTAATTTCGGTCACATC
GCCCTTGAGGTCCCGGACATTTACGCCCAATGCCAGCGCCTGGTCGATCATGACGTCGAAATCCGACGTCCACCCCGTGA
CGGCAAGATGGCGTTCGTGAGATCGCCGGATGGAATTTCCATCGAGTTGCTTCAGGCTGGCGCACCGTTGCCGCCTGCCG
AACCTTGGCAGTCGATGCCGAATTCTGGAACCTGGTGA

Upstream 100 bases:

>100_bases
GAGGATGACGTTGTCCGAGCCAGGCGCGAGAGTGTTCCAATCGCCACTCTCTGAAAGCCTCATTTCTCTCACCAATCAGC
CGGCACAAGGAGTGTGCGAG

Downstream 100 bases:

>100_bases
CCTGTGACCAGTCTGTAAAGGGAGGTGTGATTCATGAATACGGTAAATATAACCCGTCTCAAAAGCGGGGTATGTCTGAT
TGGAAGCCTTCGAGAGATCG

Product: lactoylglutathione lyase

Products: NA

Alternate protein names: Aldoketomutase; Glyoxalase I; Glx I; Ketone-aldehyde mutase; Methylglyoxalase; S-D-lactoylglutathione methylglyoxal lyase [H]

Number of amino acids: Translated: 145; Mature: 145

Protein sequence:

>145_residues
MRFLHTMVRVSDIEDSLAFYCGALGLKEVRRTENATGKFTLIFLAAPGDAEAADKHAPLVELTHNWNQEIYTGGRNFGHI
ALEVPDIYAQCQRLVDHDVEIRRPPRDGKMAFVRSPDGISIELLQAGAPLPPAEPWQSMPNSGTW

Sequences:

>Translated_145_residues
MRFLHTMVRVSDIEDSLAFYCGALGLKEVRRTENATGKFTLIFLAAPGDAEAADKHAPLVELTHNWNQEIYTGGRNFGHI
ALEVPDIYAQCQRLVDHDVEIRRPPRDGKMAFVRSPDGISIELLQAGAPLPPAEPWQSMPNSGTW
>Mature_145_residues
MRFLHTMVRVSDIEDSLAFYCGALGLKEVRRTENATGKFTLIFLAAPGDAEAADKHAPLVELTHNWNQEIYTGGRNFGHI
ALEVPDIYAQCQRLVDHDVEIRRPPRDGKMAFVRSPDGISIELLQAGAPLPPAEPWQSMPNSGTW

Specific function: Catalyzes the conversion of hemimercaptal, formed from methylglyoxal and glutathione, to S-lactoylglutathione [H]

COG id: COG0346

COG function: function code E; Lactoylglutathione lyase and related lyases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glyoxalase I family [H]

Homologues:

Organism=Homo sapiens, GI118402586, Length=144, Percent_Identity=38.1944444444444, Blast_Score=85, Evalue=2e-17,
Organism=Escherichia coli, GI1787940, Length=130, Percent_Identity=37.6923076923077, Blast_Score=90, Evalue=5e-20,
Organism=Saccharomyces cerevisiae, GI6323639, Length=142, Percent_Identity=34.5070422535211, Blast_Score=75, Evalue=6e-15,
Organism=Drosophila melanogaster, GI20129743, Length=146, Percent_Identity=37.6712328767123, Blast_Score=91, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004360
- InterPro:   IPR004361
- InterPro:   IPR018146 [H]

Pfam domain/function: PF00903 Glyoxalase [H]

EC number: =4.4.1.5 [H]

Molecular weight: Translated: 16141; Mature: 16141

Theoretical pI: Translated: 5.57; Mature: 5.57

Prosite motif: PS00934 GLYOXALASE_I_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRFLHTMVRVSDIEDSLAFYCGALGLKEVRRTENATGKFTLIFLAAPGDAEAADKHAPLV
CCHHHHHHHHHCCHHHHHHHHHHCCHHHHHHCCCCCCCEEEEEEECCCCCCCCCCCCCEE
ELTHNWNQEIYTGGRNFGHIALEVPDIYAQCQRLVDHDVEIRRPPRDGKMAFVRSPDGIS
EECCCCCCCEEECCCCCCEEEEECCHHHHHHHHHHCCCCEECCCCCCCCEEEEECCCCCE
IELLQAGAPLPPAEPWQSMPNSGTW
EEEEECCCCCCCCCHHHCCCCCCCC
>Mature Secondary Structure
MRFLHTMVRVSDIEDSLAFYCGALGLKEVRRTENATGKFTLIFLAAPGDAEAADKHAPLV
CCHHHHHHHHHCCHHHHHHHHHHCCHHHHHHCCCCCCCEEEEEEECCCCCCCCCCCCCEE
ELTHNWNQEIYTGGRNFGHIALEVPDIYAQCQRLVDHDVEIRRPPRDGKMAFVRSPDGIS
EECCCCCCCEEECCCCCCEEEEECCHHHHHHHHHHCCCCEECCCCCCCCEEEEECCCCCE
IELLQAGAPLPPAEPWQSMPNSGTW
EEEEECCCCCCCCCHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]