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The map label for this gene is oprM [H]

Identifier: 162148623

GI number: 162148623

Start: 2940737

End: 2942146

Strand: Direct

Name: oprM [H]

Synonym: GDI_2850

Alternate gene names: 162148623

Gene position: 2940737-2942146 (Clockwise)

Preceding gene: 162148622

Following gene: 162148632

Centisome position: 74.56

GC content: 65.25

Gene sequence:

>1410_bases
GTGAACATGACGCGCACGGCCTCCATTCTTGCACTCATCGTCATCGCCGGCTGCACCGTCGGGCCGGATTATGTCGCGCC
CCATGCGGAGGCTCCACCCCAATGGCTGGGCCAGCCCGGCGTTCCAAGCCAGTCGGTTCCAGATGAACGCTGGTGGGCCG
GATTCCACGACCCGCAACTGAGCCGCCTCATCCATGAGGCGCTGATACGCAACAACGATCTTGCCGCCGCCGAGAAACGT
GTCGAGGCTGCCCGCGACCAAATCCGGATCGAAGCGGCGGCGTCGTCGCCCGTCATCGGCCTTGGCGGCATCGCCGAGAA
CAGACGTCAGTCGCAGACGATCCAGTGGCCTCCCAGAGCGAGTGCGGGAGATTACCGGTATTGGTCTCTCGGGTTCGACG
GTTCATGGGAACTGGACCTGTTCGGGATGACGCGGCGCCGGACGGAAGCCGCGCGCGCATCCCAGGCCGAAATGGCGGCC
GAACGCGACGGGCTGCGCCTGCGCCTGCTCGCGGAGGTCGGCGCGGATTATGTGACGTGGTGCGCAGCCCTGGCCCGGCT
TCAGGTCGCAGAGGACCGTATCGCCCTTGTCGCTCACCAGCGCGATCTCGTGACACATGAGTGGCGTGCCGGTGCGCGCG
GCCACGCCGATGTCGATCAGGCCGAAGCGGATCTCGCCGCCGCCCAACAGACTGTACCGCCCCTTGTCGCGCAGGCCACA
GAGATGAAGAACGCCCTCGCGATCCTGACCGCAACGACACCCGAGGCCATGCAGTTGACGCCGCGCGCGGATTTTTCCAT
CCCTCTACCGCCAGCGCGGCCGCCGTCACTTCCCTCCGAGATGCTGACGCGCCGGCCGGATATCCAGGCCGCGACATTCC
GCTATGCGCGGGACAATGCGGAGATCGGGGTCGCGGTCGCCTCCCTGTACCCGAAAGTCGCGATCCCACTGGATTTCGGA
GTGACCACCAGCACGCTGCATCAGGCGTTCACCACCGCCAGCCTTCTGTGGCAACTCGGCGTTACGGCCGGCGAGACGAT
CTACGCCGGAGGGCGTCGCACCGCGCAGATCGACGCGGCGCGCCTGCAACGTGACGCAGCCTGGCTCGGTTACCGGCAGG
CCGTGCTCAACGCGATGCGCGATGTGGAAGACCGGCTCGCCACGATAGGCGACGCCAGCGCAAGAACGTCTTCGGTGAAC
CTCGAACTCGCCTCAGCCCGAGAACGCCTCATGCATACAGAGCGGGAATATCGAAGCGGCGAAGCTTCGCTCCTTCCGAC
GATTGTCGACAGAGACCTTGTGGATCAAATCCACGACCGTGTGATAGCCACGCAGCTGGCTTTGGCGATCAGCGATCTGG
CGCTGTACAAGTCATTGGGGGGCGGATGGGAAACGAACTCAACACGCTAA

Upstream 100 bases:

>100_bases
TTTCGACGCTGCTGGCCGACCTCGCGCTGATGCTGGCCGCAACCTGTGTCATTGCTGCCTGCATGCCCCCGGCCGGAGCC
GATCGGCCGGAGGTGCGATC

Downstream 100 bases:

>100_bases
AAGCCACGAAAAGCTCTCGCACCGGATGTACGTGCTCTCTATCGGGCGTTACCGGATAATAACTGAACAAGAGTCCTTTT
CAATTTAGCCATTCCGTCGC

Product: multidrug efflux MFS outer membrane protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 469; Mature: 469

Protein sequence:

>469_residues
MNMTRTASILALIVIAGCTVGPDYVAPHAEAPPQWLGQPGVPSQSVPDERWWAGFHDPQLSRLIHEALIRNNDLAAAEKR
VEAARDQIRIEAAASSPVIGLGGIAENRRQSQTIQWPPRASAGDYRYWSLGFDGSWELDLFGMTRRRTEAARASQAEMAA
ERDGLRLRLLAEVGADYVTWCAALARLQVAEDRIALVAHQRDLVTHEWRAGARGHADVDQAEADLAAAQQTVPPLVAQAT
EMKNALAILTATTPEAMQLTPRADFSIPLPPARPPSLPSEMLTRRPDIQAATFRYARDNAEIGVAVASLYPKVAIPLDFG
VTTSTLHQAFTTASLLWQLGVTAGETIYAGGRRTAQIDAARLQRDAAWLGYRQAVLNAMRDVEDRLATIGDASARTSSVN
LELASARERLMHTEREYRSGEASLLPTIVDRDLVDQIHDRVIATQLALAISDLALYKSLGGGWETNSTR

Sequences:

>Translated_469_residues
MNMTRTASILALIVIAGCTVGPDYVAPHAEAPPQWLGQPGVPSQSVPDERWWAGFHDPQLSRLIHEALIRNNDLAAAEKR
VEAARDQIRIEAAASSPVIGLGGIAENRRQSQTIQWPPRASAGDYRYWSLGFDGSWELDLFGMTRRRTEAARASQAEMAA
ERDGLRLRLLAEVGADYVTWCAALARLQVAEDRIALVAHQRDLVTHEWRAGARGHADVDQAEADLAAAQQTVPPLVAQAT
EMKNALAILTATTPEAMQLTPRADFSIPLPPARPPSLPSEMLTRRPDIQAATFRYARDNAEIGVAVASLYPKVAIPLDFG
VTTSTLHQAFTTASLLWQLGVTAGETIYAGGRRTAQIDAARLQRDAAWLGYRQAVLNAMRDVEDRLATIGDASARTSSVN
LELASARERLMHTEREYRSGEASLLPTIVDRDLVDQIHDRVIATQLALAISDLALYKSLGGGWETNSTR
>Mature_469_residues
MNMTRTASILALIVIAGCTVGPDYVAPHAEAPPQWLGQPGVPSQSVPDERWWAGFHDPQLSRLIHEALIRNNDLAAAEKR
VEAARDQIRIEAAASSPVIGLGGIAENRRQSQTIQWPPRASAGDYRYWSLGFDGSWELDLFGMTRRRTEAARASQAEMAA
ERDGLRLRLLAEVGADYVTWCAALARLQVAEDRIALVAHQRDLVTHEWRAGARGHADVDQAEADLAAAQQTVPPLVAQAT
EMKNALAILTATTPEAMQLTPRADFSIPLPPARPPSLPSEMLTRRPDIQAATFRYARDNAEIGVAVASLYPKVAIPLDFG
VTTSTLHQAFTTASLLWQLGVTAGETIYAGGRRTAQIDAARLQRDAAWLGYRQAVLNAMRDVEDRLATIGDASARTSSVN
LELASARERLMHTEREYRSGEASLLPTIVDRDLVDQIHDRVIATQLALAISDLALYKSLGGGWETNSTR

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor. Note=Attached to the outer membrane by a lipid anchor and via the transmembrane beta-barrel. The membrane anchor is not necessary for antibiotic efflux. In one report (PubMed:10889211) the Cys-18- Gly mutant is reported

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI1786785, Length=457, Percent_Identity=25.8205689277899, Blast_Score=150, Evalue=1e-37,
Organism=Escherichia coli, GI2367348, Length=478, Percent_Identity=27.4058577405858, Blast_Score=114, Evalue=2e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010131 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 51169; Mature: 51169

Theoretical pI: Translated: 5.88; Mature: 5.88

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNMTRTASILALIVIAGCTVGPDYVAPHAEAPPQWLGQPGVPSQSVPDERWWAGFHDPQL
CCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCHHHCCCCCCHHH
SRLIHEALIRNNDLAAAEKRVEAARDQIRIEAAASSPVIGLGGIAENRRQSQTIQWPPRA
HHHHHHHHHCCCCHHHHHHHHHHHHHHEEEEECCCCCCEEECCCHHCHHHCCCCCCCCCC
SAGDYRYWSLGFDGSWELDLFGMTRRRTEAARASQAEMAAERDGLRLRLLAEVGADYVTW
CCCCCEEEEECCCCCCEEEEECCHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHCCHHHHH
CAALARLQVAEDRIALVAHQRDLVTHEWRAGARGHADVDQAEADLAAAQQTVPPLVAQAT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHH
EMKNALAILTATTPEAMQLTPRADFSIPLPPARPPSLPSEMLTRRPDIQAATFRYARDNA
HHCCEEEEEEECCCHHEEECCCCCCCCCCCCCCCCCCCHHHHHCCCCCCHHHHHHHCCCC
EIGVAVASLYPKVAIPLDFGVTTSTLHQAFTTASLLWQLGVTAGETIYAGGRRTAQIDAA
CCEEEHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCEEEHHHH
RLQRDAAWLGYRQAVLNAMRDVEDRLATIGDASARTSSVNLELASARERLMHTEREYRSG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEHHHHHHHHHHHHHHHCCC
EASLLPTIVDRDLVDQIHDRVIATQLALAISDLALYKSLGGGWETNSTR
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MNMTRTASILALIVIAGCTVGPDYVAPHAEAPPQWLGQPGVPSQSVPDERWWAGFHDPQL
CCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCHHHCCCCCCHHH
SRLIHEALIRNNDLAAAEKRVEAARDQIRIEAAASSPVIGLGGIAENRRQSQTIQWPPRA
HHHHHHHHHCCCCHHHHHHHHHHHHHHEEEEECCCCCCEEECCCHHCHHHCCCCCCCCCC
SAGDYRYWSLGFDGSWELDLFGMTRRRTEAARASQAEMAAERDGLRLRLLAEVGADYVTW
CCCCCEEEEECCCCCCEEEEECCHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHCCHHHHH
CAALARLQVAEDRIALVAHQRDLVTHEWRAGARGHADVDQAEADLAAAQQTVPPLVAQAT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHH
EMKNALAILTATTPEAMQLTPRADFSIPLPPARPPSLPSEMLTRRPDIQAATFRYARDNA
HHCCEEEEEEECCCHHEEECCCCCCCCCCCCCCCCCCCHHHHHCCCCCCHHHHHHHCCCC
EIGVAVASLYPKVAIPLDFGVTTSTLHQAFTTASLLWQLGVTAGETIYAGGRRTAQIDAA
CCEEEHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCEEEHHHH
RLQRDAAWLGYRQAVLNAMRDVEDRLATIGDASARTSSVNLELASARERLMHTEREYRSG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEHHHHHHHHHHHHHHHCCC
EASLLPTIVDRDLVDQIHDRVIATQLALAISDLALYKSLGGGWETNSTR
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8226684; 10889211; 10984043; 7968531; 8540696; 9401051; 9603892; 10952562 [H]