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The map label for this gene is gst [H]

Identifier: 162148544

GI number: 162148544

Start: 2856490

End: 2857188

Strand: Reverse

Name: gst [H]

Synonym: GDI_2768

Alternate gene names: 162148544

Gene position: 2857188-2856490 (Counterclockwise)

Preceding gene: 162148545

Following gene: 162148543

Centisome position: 72.44

GC content: 63.23

Gene sequence:

>699_bases
ATGATCCGCTTCTACTACCACCCGACGCCGAACCCCGCGAAAATCGCGCTGTTCCTGGAGGAGGCTGGACTTTCCTATGA
AGCCGTGCCGATCGATACCAGCAAGGGGGAGCAGCACGCGCCGGCTTTCCGCAGGATCAACCCGAACGGCAAGCTTCCGG
CCATCGTCGATACGGACGGCCCCGGCGGCCAGGAAGCCCGCGTCTTCGATTCCACGGCCATTCTTCTCTATCTGGCCGAA
AAGACGGATCGCTTCCTCGGCACCGACGCCGACCGCCCGGAACTCCTGTCCTGGCTGATGTTTCTGGGCACCGGCCTCGG
CCCGTTTTCCGGTCAGGCCGTGCATTTCCAGTATGCCGCGCCCGGCGGACTCGACTATGCCGTCAACCGCTACCGACGGG
AAACAGAGCGGCATTACCAGGTGCTGAACGACCATCTGGAAGGCCGCTCCTTCGTCGTCGGCGAGGATTATACCATTGCC
GACATGTCGGCATGGGGCTGGCTCGACCGCGCGCCGCGCGTGATGAAAGGGTCAGACACACCGCTGGCACCCTGGCCCAA
CCTGGCGCGCTTCATGAACCTCATGAACGCACGTCCGGCCGTTGCCCGTGCCCGCGCCGTCATGACCACCCATACTTTCA
AGACCGAGATGGACGTGCAAGCGTTACGCGCGCTCTATCCCTCGAACTTCCCGGCATAA

Upstream 100 bases:

>100_bases
GGCTGAATGAAACGCCGGTCCCGCACCGGTCTTCTCCACAACAGTCGTCATTTTCCCCACCATGAGTATCCCGGCCGACA
TATCGAAAGGATGGACGCCC

Downstream 100 bases:

>100_bases
TCGCGAAAGGTCCGTTACATGTCTTCATCGAATACCTACACCCCGCCGCCGGTCTGGACATGGAACGCCCAGAACGGTGG
CACATTCGCCAGCATCAACC

Product: glutathionine S-transferase

Products: HX; R-S-glutathione

Alternate protein names: GST-like protein yfcG [H]

Number of amino acids: Translated: 232; Mature: 232

Protein sequence:

>232_residues
MIRFYYHPTPNPAKIALFLEEAGLSYEAVPIDTSKGEQHAPAFRRINPNGKLPAIVDTDGPGGQEARVFDSTAILLYLAE
KTDRFLGTDADRPELLSWLMFLGTGLGPFSGQAVHFQYAAPGGLDYAVNRYRRETERHYQVLNDHLEGRSFVVGEDYTIA
DMSAWGWLDRAPRVMKGSDTPLAPWPNLARFMNLMNARPAVARARAVMTTHTFKTEMDVQALRALYPSNFPA

Sequences:

>Translated_232_residues
MIRFYYHPTPNPAKIALFLEEAGLSYEAVPIDTSKGEQHAPAFRRINPNGKLPAIVDTDGPGGQEARVFDSTAILLYLAE
KTDRFLGTDADRPELLSWLMFLGTGLGPFSGQAVHFQYAAPGGLDYAVNRYRRETERHYQVLNDHLEGRSFVVGEDYTIA
DMSAWGWLDRAPRVMKGSDTPLAPWPNLARFMNLMNARPAVARARAVMTTHTFKTEMDVQALRALYPSNFPA
>Mature_232_residues
MIRFYYHPTPNPAKIALFLEEAGLSYEAVPIDTSKGEQHAPAFRRINPNGKLPAIVDTDGPGGQEARVFDSTAILLYLAE
KTDRFLGTDADRPELLSWLMFLGTGLGPFSGQAVHFQYAAPGGLDYAVNRYRRETERHYQVLNDHLEGRSFVVGEDYTIA
DMSAWGWLDRAPRVMKGSDTPLAPWPNLARFMNLMNARPAVARARAVMTTHTFKTEMDVQALRALYPSNFPA

Specific function: Has disulfide bond reductase activity (in vitro). Has low hydroperoxidase activity with cumene hydroperoxide. Has very low glutathione-S-transferase activity (in vitro) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GST N-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1788640, Length=206, Percent_Identity=45.1456310679612, Blast_Score=169, Evalue=2e-43,
Organism=Escherichia coli, GI87082195, Length=228, Percent_Identity=37.719298245614, Blast_Score=124, Evalue=4e-30,
Organism=Saccharomyces cerevisiae, GI6324100, Length=240, Percent_Identity=29.1666666666667, Blast_Score=96, Evalue=6e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010987
- InterPro:   IPR004045
- InterPro:   IPR017933
- InterPro:   IPR004046
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF00043 GST_C; PF02798 GST_N [H]

EC number: 2.5.1.18

Molecular weight: Translated: 25949; Mature: 25949

Theoretical pI: Translated: 6.81; Mature: 6.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRFYYHPTPNPAKIALFLEEAGLSYEAVPIDTSKGEQHAPAFRRINPNGKLPAIVDTDG
CEEEEECCCCCCEEEEEEEECCCCCEEEEECCCCCCCCCCCHHEEECCCCCCCEEEECCC
PGGQEARVFDSTAILLYLAEKTDRFLGTDADRPELLSWLMFLGTGLGPFSGQAVHFQYAA
CCCCCEEEECCEEEEEEEECHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCEEEEEECC
PGGLDYAVNRYRRETERHYQVLNDHLEGRSFVVGEDYTIADMSAWGWLDRAPRVMKGSDT
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCEEECCHHCCCHHCCCHHHCCCCC
PLAPWPNLARFMNLMNARPAVARARAVMTTHTFKTEMDVQALRALYPSNFPA
CCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MIRFYYHPTPNPAKIALFLEEAGLSYEAVPIDTSKGEQHAPAFRRINPNGKLPAIVDTDG
CEEEEECCCCCCEEEEEEEECCCCCEEEEECCCCCCCCCCCHHEEECCCCCCCEEEECCC
PGGQEARVFDSTAILLYLAEKTDRFLGTDADRPELLSWLMFLGTGLGPFSGQAVHFQYAA
CCCCCEEEECCEEEEEEEECHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCEEEEEECC
PGGLDYAVNRYRRETERHYQVLNDHLEGRSFVVGEDYTIADMSAWGWLDRAPRVMKGSDT
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCEEECCHHCCCHHCCCHHHCCCCC
PLAPWPNLARFMNLMNARPAVARARAVMTTHTFKTEMDVQALRALYPSNFPA
CCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: RX; glutathione

Specific reaction: RX + glutathione = HX + R-S-glutathione

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]