Click here to switch to the map view.

The map label for this gene is bedA [H]

Identifier: 162148461

GI number: 162148461

Start: 2776893

End: 2778407

Strand: Direct

Name: bedA [H]

Synonym: GDI_2681

Alternate gene names: 162148461

Gene position: 2776893-2778407 (Clockwise)

Preceding gene: 162148460

Following gene: 162148462

Centisome position: 70.41

GC content: 67.13

Gene sequence:

>1515_bases
GTGACGCAAGACAGTCCATATCGCCGTGACGCGCCGTGGCACGACGTCGCGCCCCTTGAAGATCTCGATCCCGCCATTGC
GCGCCGTGTGGAGGTCGAGGGTCACCCGGTGCTTCTGCTTCAGCGGGAGAACGGGCAGGTGCGTGCCCTTGCCGCGGAAT
GCCCGCATAAGGGCGCGCCGCTGGAAAAGGGTGTCGTATGCGGCGACCGGCTGGTCTGCCCATGGCACAAGGCGATCTTT
TCGGTCGAGGATGGGCGGCTGCTCGAGCCCCTTGCCCTTGATCCTCTGGCATCCTACCCGACCGAGATCCGCGACGGGCG
CGTCCTTGTCTCCCTCGGCGCACGCCCGCTATCGTCCGGGACCGAGGGGCCGGCGCAGAGCGTCCTGATCGTGGGGGCCG
GAGCCGCCGGTGTCGCCGCCGCTGTCTCGCTGCGCGAATTCGGCTTTGCCGGCTCGGTGACCATGGTCGATAGTCAGGAT
GCGGCGCCTTACGATCGCACGGCGCTGACCAAGGCCGTACTGGCCGGCAAGGCCGACGGTGACATGCCCCCGTTACTGCG
TCCGGACGGCTTCTGGACCGATCACGGCATCGATCGCGTCGTGGGCCTGGTCGTGGCACTGGATGCGCCGTCGCGCGCCA
TCACGCTGGCGGACGGGCGGGTTCTGGCGGCCGATCATGTGCTGCTTGCCCCCGGCTCCACTCCCCGCCCCCTGGACGTA
CCCGGTGCCGATCTGCCGGGCGTATTCACGCTTCGGCAGACAGTCGATGCACAGCGAATGCTCGCGGCCTGTCCTCCGAA
CGGACGGGTCGTGATCAGCGGGGGAAATTTCATCGGCATGGAGGCCGCCGCATCGCTGACATCCCGGGGCATGCAGGTAA
CGATCATCGCACCGGACGATATCCCGTTCGCGAAAATGTTCGGCCGCGAGGTCGGCGCGCGGCTGCGGCGCCTGCATGAG
GAAAACGGCGTGGTCTACAGCGGTGGACGACGCATCGTCGGAATGGAGGGAGACCAGCGTGTCGAGAGGGTCTCTCTCGA
CGACGGTACCAGTCTGGAAGACGAAATCGTCCTGGTGGGTGTCGGCGTGCAACCCGCTACCGGTTTCGCGGCGGCACTGG
CGGCCCCGGATGGCGGCATCGATGTCGACGACCGCATGCAGGCCCTTCCCGGCATCTCCGTCGCCGGGGACTGTGCACGC
ATCACGCATGGCGACAGCCGCGTCCGGATCGAGCACTGGCGCTCGGCACAGGTGCAGGGCCGTCAGGCCGCGCGGACGAT
CACGGGTCGATCCGGGCCTTCGCGTTTTCTTCCCTGGTTCTGGACCCAGCAATTCGGAAGCAAGATCGAATATGCGGGCT
ATCATGAACCATTCGACGACGTGACCATCGAAGGCGATCTGGACGCGCTTCATTTCGTTGCGCGTCTGGCCCGGAACGGC
CGCTGCGTCGGCCTGGTCACGGCGGGACGTCCGGACGTGACGGCGCACGCCGTGCTGTCCGGCAGGCTGGAGTAA

Upstream 100 bases:

>100_bases
CGAAGCCAGCTACATGACGGGCCAGGTCGTCGGCGTGAATGGAGGGGTCGGACTGCCATAATGGCGGCCGCGCCGATCAT
CTGTTTCGAGAGGATATCCA

Downstream 100 bases:

>100_bases
GGGCGCCACCAGTACGCGCCCGCCAAGAACATTCACAGGAATAGACAATAGCAGGAGGTTGGCATGACCAACGACAAAAC
ATCCATCGCGACCAGCGCCA

Product: benzene 1,2-dioxygenase system ferredoxin--NAD(+) reductase subunit

Products: oxidized rubredoxin; NADH; H+

Alternate protein names: NA

Number of amino acids: Translated: 504; Mature: 503

Protein sequence:

>504_residues
MTQDSPYRRDAPWHDVAPLEDLDPAIARRVEVEGHPVLLLQRENGQVRALAAECPHKGAPLEKGVVCGDRLVCPWHKAIF
SVEDGRLLEPLALDPLASYPTEIRDGRVLVSLGARPLSSGTEGPAQSVLIVGAGAAGVAAAVSLREFGFAGSVTMVDSQD
AAPYDRTALTKAVLAGKADGDMPPLLRPDGFWTDHGIDRVVGLVVALDAPSRAITLADGRVLAADHVLLAPGSTPRPLDV
PGADLPGVFTLRQTVDAQRMLAACPPNGRVVISGGNFIGMEAAASLTSRGMQVTIIAPDDIPFAKMFGREVGARLRRLHE
ENGVVYSGGRRIVGMEGDQRVERVSLDDGTSLEDEIVLVGVGVQPATGFAAALAAPDGGIDVDDRMQALPGISVAGDCAR
ITHGDSRVRIEHWRSAQVQGRQAARTITGRSGPSRFLPWFWTQQFGSKIEYAGYHEPFDDVTIEGDLDALHFVARLARNG
RCVGLVTAGRPDVTAHAVLSGRLE

Sequences:

>Translated_504_residues
MTQDSPYRRDAPWHDVAPLEDLDPAIARRVEVEGHPVLLLQRENGQVRALAAECPHKGAPLEKGVVCGDRLVCPWHKAIF
SVEDGRLLEPLALDPLASYPTEIRDGRVLVSLGARPLSSGTEGPAQSVLIVGAGAAGVAAAVSLREFGFAGSVTMVDSQD
AAPYDRTALTKAVLAGKADGDMPPLLRPDGFWTDHGIDRVVGLVVALDAPSRAITLADGRVLAADHVLLAPGSTPRPLDV
PGADLPGVFTLRQTVDAQRMLAACPPNGRVVISGGNFIGMEAAASLTSRGMQVTIIAPDDIPFAKMFGREVGARLRRLHE
ENGVVYSGGRRIVGMEGDQRVERVSLDDGTSLEDEIVLVGVGVQPATGFAAALAAPDGGIDVDDRMQALPGISVAGDCAR
ITHGDSRVRIEHWRSAQVQGRQAARTITGRSGPSRFLPWFWTQQFGSKIEYAGYHEPFDDVTIEGDLDALHFVARLARNG
RCVGLVTAGRPDVTAHAVLSGRLE
>Mature_503_residues
TQDSPYRRDAPWHDVAPLEDLDPAIARRVEVEGHPVLLLQRENGQVRALAAECPHKGAPLEKGVVCGDRLVCPWHKAIFS
VEDGRLLEPLALDPLASYPTEIRDGRVLVSLGARPLSSGTEGPAQSVLIVGAGAAGVAAAVSLREFGFAGSVTMVDSQDA
APYDRTALTKAVLAGKADGDMPPLLRPDGFWTDHGIDRVVGLVVALDAPSRAITLADGRVLAADHVLLAPGSTPRPLDVP
GADLPGVFTLRQTVDAQRMLAACPPNGRVVISGGNFIGMEAAASLTSRGMQVTIIAPDDIPFAKMFGREVGARLRRLHEE
NGVVYSGGRRIVGMEGDQRVERVSLDDGTSLEDEIVLVGVGVQPATGFAAALAAPDGGIDVDDRMQALPGISVAGDCARI
THGDSRVRIEHWRSAQVQGRQAARTITGRSGPSRFLPWFWTQQFGSKIEYAGYHEPFDDVTIEGDLDALHFVARLARNGR
CVGLVTAGRPDVTAHAVLSGRLE

Specific function: The degradation of the thiocarbamate herbicide EPTC by cytochrome CYP116 (thcB) requires the participation of a flavoprotein, rhodocoxin reductase, and an iron-sulfur protein, rhodocoxin, to mediate the transfer of electrons from NADH to P450 for oxygen a

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI21389617, Length=527, Percent_Identity=33.2068311195446, Blast_Score=267, Evalue=2e-71,
Organism=Homo sapiens, GI65787454, Length=527, Percent_Identity=33.2068311195446, Blast_Score=266, Evalue=2e-71,
Organism=Homo sapiens, GI226437568, Length=527, Percent_Identity=33.2068311195446, Blast_Score=266, Evalue=3e-71,
Organism=Homo sapiens, GI22202629, Length=334, Percent_Identity=26.0479041916168, Blast_Score=69, Evalue=1e-11,
Organism=Homo sapiens, GI4757732, Length=334, Percent_Identity=26.0479041916168, Blast_Score=68, Evalue=2e-11,
Organism=Escherichia coli, GI1788892, Length=376, Percent_Identity=29.7872340425532, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI1789765, Length=254, Percent_Identity=26.3779527559055, Blast_Score=66, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI17559934, Length=500, Percent_Identity=27.6, Blast_Score=197, Evalue=2e-50,
Organism=Drosophila melanogaster, GI24639257, Length=514, Percent_Identity=31.3229571984436, Blast_Score=233, Evalue=2e-61,
Organism=Drosophila melanogaster, GI281359715, Length=514, Percent_Identity=31.3229571984436, Blast_Score=233, Evalue=3e-61,
Organism=Drosophila melanogaster, GI281359713, Length=514, Percent_Identity=31.3229571984436, Blast_Score=233, Evalue=3e-61,
Organism=Drosophila melanogaster, GI24639250, Length=514, Percent_Identity=31.3229571984436, Blast_Score=233, Evalue=3e-61,
Organism=Drosophila melanogaster, GI18543267, Length=514, Percent_Identity=31.3229571984436, Blast_Score=233, Evalue=3e-61,
Organism=Drosophila melanogaster, GI24639252, Length=514, Percent_Identity=31.3229571984436, Blast_Score=233, Evalue=3e-61,
Organism=Drosophila melanogaster, GI24585130, Length=467, Percent_Identity=31.9057815845824, Blast_Score=230, Evalue=2e-60,
Organism=Drosophila melanogaster, GI28573993, Length=368, Percent_Identity=26.0869565217391, Blast_Score=95, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24581020, Length=364, Percent_Identity=25.8241758241758, Blast_Score=94, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR004099
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.18.1.1

Molecular weight: Translated: 53499; Mature: 53368

Theoretical pI: Translated: 5.47; Mature: 5.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQDSPYRRDAPWHDVAPLEDLDPAIARRVEVEGHPVLLLQRENGQVRALAAECPHKGAP
CCCCCCCCCCCCCCCCCCHHHCCHHHHHEEEECCCCEEEEECCCCCEEEEEECCCCCCCC
LEKGVVCGDRLVCPWHKAIFSVEDGRLLEPLALDPLASYPTEIRDGRVLVSLGARPLSSG
CCCCEECCCCEECCHHHHEEEECCCCEECCCCCCCHHCCCCCCCCCEEEEEECCCCCCCC
TEGPAQSVLIVGAGAAGVAAAVSLREFGFAGSVTMVDSQDAAPYDRTALTKAVLAGKADG
CCCCCCEEEEEECCCHHHHHHHHHHHCCCCCCEEEEECCCCCCCCHHHHHHHHHHCCCCC
DMPPLLRPDGFWTDHGIDRVVGLVVALDAPSRAITLADGRVLAADHVLLAPGSTPRPLDV
CCCCCCCCCCCCCCCCHHHHEEEEEEECCCCCEEEEECCEEEEECEEEECCCCCCCCCCC
PGADLPGVFTLRQTVDAQRMLAACPPNGRVVISGGNFIGMEAAASLTSRGMQVTIIAPDD
CCCCCCCEEEHHHHHCHHHHHHCCCCCCEEEEECCCEECHHHHHHHHHCCEEEEEECCCC
IPFAKMFGREVGARLRRLHEENGVVYSGGRRIVGMEGDQRVERVSLDDGTSLEDEIVLVG
CCHHHHHHHHHHHHHHHHHHHCCEEEECCCEEEECCCCCCEEEEECCCCCCCCCCEEEEE
VGVQPATGFAAALAAPDGGIDVDDRMQALPGISVAGDCARITHGDSRVRIEHWRSAQVQG
ECCCCCCCCEEEEECCCCCCCHHHHHHHCCCCCCCCCCEEEECCCCEEEEEECCCCHHCC
RQAARTITGRSGPSRFLPWFWTQQFGSKIEYAGYHEPFDDVTIEGDLDALHFVARLARNG
HHHHHEECCCCCCCCCCCHHHHHHHCCEECCCCCCCCCCCEEEECCHHHHHHHHHHHCCC
RCVGLVTAGRPDVTAHAVLSGRLE
CEEEEEECCCCCCEEEEEEECCCC
>Mature Secondary Structure 
TQDSPYRRDAPWHDVAPLEDLDPAIARRVEVEGHPVLLLQRENGQVRALAAECPHKGAP
CCCCCCCCCCCCCCCCCHHHCCHHHHHEEEECCCCEEEEECCCCCEEEEEECCCCCCCC
LEKGVVCGDRLVCPWHKAIFSVEDGRLLEPLALDPLASYPTEIRDGRVLVSLGARPLSSG
CCCCEECCCCEECCHHHHEEEECCCCEECCCCCCCHHCCCCCCCCCEEEEEECCCCCCCC
TEGPAQSVLIVGAGAAGVAAAVSLREFGFAGSVTMVDSQDAAPYDRTALTKAVLAGKADG
CCCCCCEEEEEECCCHHHHHHHHHHHCCCCCCEEEEECCCCCCCCHHHHHHHHHHCCCCC
DMPPLLRPDGFWTDHGIDRVVGLVVALDAPSRAITLADGRVLAADHVLLAPGSTPRPLDV
CCCCCCCCCCCCCCCCHHHHEEEEEEECCCCCEEEEECCEEEEECEEEECCCCCCCCCCC
PGADLPGVFTLRQTVDAQRMLAACPPNGRVVISGGNFIGMEAAASLTSRGMQVTIIAPDD
CCCCCCCEEEHHHHHCHHHHHHCCCCCCEEEEECCCEECHHHHHHHHHCCEEEEEECCCC
IPFAKMFGREVGARLRRLHEENGVVYSGGRRIVGMEGDQRVERVSLDDGTSLEDEIVLVG
CCHHHHHHHHHHHHHHHHHHHCCEEEECCCEEEECCCCCCEEEEECCCCCCCCCCEEEEE
VGVQPATGFAAALAAPDGGIDVDDRMQALPGISVAGDCARITHGDSRVRIEHWRSAQVQG
ECCCCCCCCEEEEECCCCCCCHHHHHHHCCCCCCCCCCEEEECCCCEEEEEECCCCHHCC
RQAARTITGRSGPSRFLPWFWTQQFGSKIEYAGYHEPFDDVTIEGDLDALHFVARLARNG
HHHHHEECCCCCCCCCCCHHHHHHHCCEECCCCCCCCCCCEEEECCHHHHHHHHHHHCCC
RCVGLVTAGRPDVTAHAVLSGRLE
CEEEEEECCCCCCEEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: reduced rubredoxin; NAD+

Specific reaction: reduced rubredoxin + NAD+ = oxidized rubredoxin + NADH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7836301 [H]