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The map label for this gene is mdlA [H]

Identifier: 162148446

GI number: 162148446

Start: 2757736

End: 2759016

Strand: Reverse

Name: mdlA [H]

Synonym: GDI_2665

Alternate gene names: 162148446

Gene position: 2759016-2757736 (Counterclockwise)

Preceding gene: 162148447

Following gene: 162148445

Centisome position: 69.95

GC content: 64.17

Gene sequence:

>1281_bases
ATGACCCGGATCACCGGCCTGCGCGTGTTCGATATCCGCTTTCCGACATCCGCCCGGCTCGACGGGTCGGACGCCATGAA
TCCGGACCCGGACTATTCCGCCGCCTATGTGACCCTCCAGACCGACGATCCGCAGATCGAGGGATACGGGCTGACCTTCA
CCATAGGCCGGGGCAACGAACTGTGCTGCGCAGCCATCAGCGCGCTGGCGCCGCTTGTCGTCGGCCTGGACCTGGACTGG
ATCAGGGAAAATCCGGGACGATTCTGGCGCCATGTGACGGGTGACAGCCAGTTGCGCTGGGTCGGCCCGGACAAGGGCGT
GATCCATATGGCGACCGGCGCGGTGGTGAACGCGGTGTGGGACCTGTGGGCGCGGGCCGAGGGAAAACCCGTCTGGCAAC
TGGTGGCGGAGATGTCGCCCGCCGAACTGGTCCGCGCGATCGACTTCCGCTACCTGACCGATTGCATCACGCCCGAGGAG
GCGCTGGACCGCCTGACCGATCGTGCCGCCGGAAAGCGGGCGCGCATCGAGACGCTGCTTGCCGATGGTTATCCCTGCTA
CACGACATCGGCCGGCTGGCTCGGTTATGACGACGACAAGCTGCGCGGCCTCTGTCGCGATGCCGTGCGGGCGGGATTCG
CCCATATCAAGCTCAAGGTCGGGCAGAATCTGGCGGATGATGTCCGCCGGGTATGGATCGCGCGTGAGGAAATCGGTCCC
GACCGCCGGCTGATGATCGACGCGAACCAGGTCTGGGAACGGGACCAGGCGATCGAATGGGTTCGTGCGCTGTCCTTCGC
CAACCCGTGGTTTATCGAAGAACCCACGTCACCGGACGACGTGGAGGCGCATCGCGCGATCCGCGAAGCCGTCGCGCCCG
TCGCGGTGGCTACCGGCGAGATGTGCCAGAATCGCGTGATGTTCAAGCAGTTCATCATGCGCCAGGCCATCGACGTGGTC
CAGATCGACTCGTGCCGGGTGGGGGGGATGAACGAAATCCTGGCAATCCTGCTGATGGCCGACAAATACGGCCTGCCGGT
CTGCCCCCATGCGGGGGGGGTCGGATTGTGCGAATATGTCCAGCATTTTTCGATGATCGACTACGCCGCGATTTCCGGAA
GCATGGCGGGCCGTGTAATTGAATATGTCGATCATCTGCATGAACATTTCACCGATCCCTGCGTGGTGCGCGGCGCACGG
TACATGCCGCCGCTGGCACCGGGTTTTTCGGTCCGCATGAAAGCCGACACGCTGCGCACCTTCCTGCATCGGGCCCCGTA
G

Upstream 100 bases:

>100_bases
GGCTCGGCCAGCGGCCGCCGGTCTATCTCCGGCCGGGACAGACCGTGCGCCTGGGCATCGAGGGACTTGGGATCCAGCAG
CAATATGTCAGGGGGGCGGC

Downstream 100 bases:

>100_bases
GTGTCATCATGGATCTTCGCCTGACCGGGCATGTCGTTATCGTGACGGGCGGTGCCTCGGGGATCGGGGCCGCGATTTCG
GAGGTGCTTGCCGAAGAGGG

Product: mandelate racemase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 426; Mature: 425

Protein sequence:

>426_residues
MTRITGLRVFDIRFPTSARLDGSDAMNPDPDYSAAYVTLQTDDPQIEGYGLTFTIGRGNELCCAAISALAPLVVGLDLDW
IRENPGRFWRHVTGDSQLRWVGPDKGVIHMATGAVVNAVWDLWARAEGKPVWQLVAEMSPAELVRAIDFRYLTDCITPEE
ALDRLTDRAAGKRARIETLLADGYPCYTTSAGWLGYDDDKLRGLCRDAVRAGFAHIKLKVGQNLADDVRRVWIAREEIGP
DRRLMIDANQVWERDQAIEWVRALSFANPWFIEEPTSPDDVEAHRAIREAVAPVAVATGEMCQNRVMFKQFIMRQAIDVV
QIDSCRVGGMNEILAILLMADKYGLPVCPHAGGVGLCEYVQHFSMIDYAAISGSMAGRVIEYVDHLHEHFTDPCVVRGAR
YMPPLAPGFSVRMKADTLRTFLHRAP

Sequences:

>Translated_426_residues
MTRITGLRVFDIRFPTSARLDGSDAMNPDPDYSAAYVTLQTDDPQIEGYGLTFTIGRGNELCCAAISALAPLVVGLDLDW
IRENPGRFWRHVTGDSQLRWVGPDKGVIHMATGAVVNAVWDLWARAEGKPVWQLVAEMSPAELVRAIDFRYLTDCITPEE
ALDRLTDRAAGKRARIETLLADGYPCYTTSAGWLGYDDDKLRGLCRDAVRAGFAHIKLKVGQNLADDVRRVWIAREEIGP
DRRLMIDANQVWERDQAIEWVRALSFANPWFIEEPTSPDDVEAHRAIREAVAPVAVATGEMCQNRVMFKQFIMRQAIDVV
QIDSCRVGGMNEILAILLMADKYGLPVCPHAGGVGLCEYVQHFSMIDYAAISGSMAGRVIEYVDHLHEHFTDPCVVRGAR
YMPPLAPGFSVRMKADTLRTFLHRAP
>Mature_425_residues
TRITGLRVFDIRFPTSARLDGSDAMNPDPDYSAAYVTLQTDDPQIEGYGLTFTIGRGNELCCAAISALAPLVVGLDLDWI
RENPGRFWRHVTGDSQLRWVGPDKGVIHMATGAVVNAVWDLWARAEGKPVWQLVAEMSPAELVRAIDFRYLTDCITPEEA
LDRLTDRAAGKRARIETLLADGYPCYTTSAGWLGYDDDKLRGLCRDAVRAGFAHIKLKVGQNLADDVRRVWIAREEIGPD
RRLMIDANQVWERDQAIEWVRALSFANPWFIEEPTSPDDVEAHRAIREAVAPVAVATGEMCQNRVMFKQFIMRQAIDVVQ
IDSCRVGGMNEILAILLMADKYGLPVCPHAGGVGLCEYVQHFSMIDYAAISGSMAGRVIEYVDHLHEHFTDPCVVRGARY
MPPLAPGFSVRMKADTLRTFLHRAP

Specific function: Probably Involved In The Degradation Of Homoserine Lactone (Hsl) Or Of A Metabolite Of Hsl That Signals Starvation. [C]

COG id: COG4948

COG function: function code MR; L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family [H]

Homologues:

Organism=Homo sapiens, GI42544119, Length=427, Percent_Identity=52.6932084309134, Blast_Score=473, Evalue=1e-133,
Organism=Homo sapiens, GI42544117, Length=406, Percent_Identity=51.4778325123153, Blast_Score=440, Evalue=1e-123,
Organism=Homo sapiens, GI186972148, Length=334, Percent_Identity=52.6946107784431, Blast_Score=387, Evalue=1e-107,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015654
- InterPro:   IPR013342
- InterPro:   IPR013341
- InterPro:   IPR001354 [H]

Pfam domain/function: PF01188 MR_MLE; PF02746 MR_MLE_N [H]

EC number: NA

Molecular weight: Translated: 47549; Mature: 47418

Theoretical pI: Translated: 5.46; Mature: 5.46

Prosite motif: PS00909 MR_MLE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRITGLRVFDIRFPTSARLDGSDAMNPDPDYSAAYVTLQTDDPQIEGYGLTFTIGRGNE
CCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCEEEEEEEEEECCCCH
LCCAAISALAPLVVGLDLDWIRENPGRFWRHVTGDSQLRWVGPDKGVIHMATGAVVNAVW
HHHHHHHHHHHHHHCCCHHHHHCCCCHHHHCCCCCCEEEEECCCCCEEEEHHHHHHHHHH
DLWARAEGKPVWQLVAEMSPAELVRAIDFRYLTDCITPEEALDRLTDRAAGKRARIETLL
HHHHCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHH
ADGYPCYTTSAGWLGYDDDKLRGLCRDAVRAGFAHIKLKVGQNLADDVRRVWIAREEIGP
HCCCCCEECCCCCCCCCHHHHHHHHHHHHHCCCEEEEEEECCCHHHHHHHHHHHHHHCCC
DRRLMIDANQVWERDQAIEWVRALSFANPWFIEEPTSPDDVEAHRAIREAVAPVAVATGE
CCEEEECHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCCHHHHHHHHHHHHHHHHHHHHH
MCQNRVMFKQFIMRQAIDVVQIDSCRVGGMNEILAILLMADKYGLPVCPHAGGVGLCEYV
HHHHHHHHHHHHHHHHCCEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHH
QHFSMIDYAAISGSMAGRVIEYVDHLHEHFTDPCVVRGARYMPPLAPGFSVRMKADTLRT
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCCCEEEEEHHHHHH
FLHRAP
HHHCCC
>Mature Secondary Structure 
TRITGLRVFDIRFPTSARLDGSDAMNPDPDYSAAYVTLQTDDPQIEGYGLTFTIGRGNE
CCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCEEEEEEEEEECCCCH
LCCAAISALAPLVVGLDLDWIRENPGRFWRHVTGDSQLRWVGPDKGVIHMATGAVVNAVW
HHHHHHHHHHHHHHCCCHHHHHCCCCHHHHCCCCCCEEEEECCCCCEEEEHHHHHHHHHH
DLWARAEGKPVWQLVAEMSPAELVRAIDFRYLTDCITPEEALDRLTDRAAGKRARIETLL
HHHHCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHH
ADGYPCYTTSAGWLGYDDDKLRGLCRDAVRAGFAHIKLKVGQNLADDVRRVWIAREEIGP
HCCCCCEECCCCCCCCCHHHHHHHHHHHHHCCCEEEEEEECCCHHHHHHHHHHHHHHCCC
DRRLMIDANQVWERDQAIEWVRALSFANPWFIEEPTSPDDVEAHRAIREAVAPVAVATGE
CCEEEECHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCCHHHHHHHHHHHHHHHHHHHHH
MCQNRVMFKQFIMRQAIDVVQIDSCRVGGMNEILAILLMADKYGLPVCPHAGGVGLCEYV
HHHHHHHHHHHHHHHHCCEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHH
QHFSMIDYAAISGSMAGRVIEYVDHLHEHFTDPCVVRGARYMPPLAPGFSVRMKADTLRT
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCCCEEEEEHHHHHH
FLHRAP
HHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9353932; 9384377 [H]