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The map label for this gene is rbsR [H]

Identifier: 162148361

GI number: 162148361

Start: 2671496

End: 2672578

Strand: Direct

Name: rbsR [H]

Synonym: GDI_2578

Alternate gene names: 162148361

Gene position: 2671496-2672578 (Clockwise)

Preceding gene: 162148359

Following gene: 162148363

Centisome position: 67.73

GC content: 70.36

Gene sequence:

>1083_bases
ATGTTGGCCGGCCAGAACTTGTACACCGCCTGCACTATCGGAAATGATACCATTATGCCGCGCGAGCGCACGCCCCGCTC
CCCCATTTCCTCCATCGACGTCGCACGGCTGGCCGGAGTATCGCAATCGGCGGTTTCACGCAGTTTTTCCCCCGGCGGCA
GCGTATCCCCCGCGATGCGCGAGAAGGTTCTGGCGGCGGCCGCCGTGCTGGGATACCGGCCGAACCTGATCCCCCGCATC
ATGCTGTCCGGCCGGTCGCGGATGATCGGCGTGGTGGTCGGGGGGCTGGACAATCCCTTCTACGCCGCCGTCCTGGATCA
TTTCGCGGTCGTCCTGCGCGCCGTGGGGCTGCAGGTCCTGCTGGTGCAGGTGCCCGATGCCCTGGCGCTGGACGGGGCGC
TGGACCAGTTGGCGGGCTACCGGGTCGATGCGGTCGTGACCGCGCTGGCCGTGGGCACGCGGCAGGCCGCGACGGCGCTG
TCGGACCTGCGGATACCGATCGTCTGTTTCAATTCCCGGCTGACAGCGCCGTGGATTTCCACCGTCCGGTCGGACAACCG
CGCGGCGGGACAGGCTGCGGCCCACCTGCTGGCCGATCGCGGGGCGCGCCGCACCGCATGGCTCGCCGGCCCGCGCGACA
GCGCCGCGACCCGCGCGCGGGCACGCGGCTTCCTGGATGGATTGGCCGCCCGCGCCCTGCCCGCGCCCGTCGTCATCGTG
GGGCAGGATACCTATCACGATGGATATGCGGCGGTGCGCGACCTGTTGCGGGGGCACCGGCCGCCGGAAGCGTTCTTCTG
CAGCAACGACATGATGGCATGCGGCGCGATCGACGCACTGCGCGCCCACGGCCTGACGGTGCCCGGCGACGTGCGGGTGG
TCGGGTACGACAACATTCCCCAGGCCGCATGGCACGGGTACGACCTGACATCGTTCGACCAGCAGGTGGACCGCATGGCG
GAAGCGGCACTGGACCTGCTGACCTCCGCCCTGGCCGACCCAGCGACAGCGGCGAAAGGGCGGACCCGCACCGTTCCCGC
CCGGCTGGTCGAACGCGGCAGTACCGCGCCACAGGCCCGATAG

Upstream 100 bases:

>100_bases
AGCGCCAGCAACAGGACCAGGATCAGCCGCGCCGACTGCTTCACGCACCGTCTTCCAGCATGATCGTGCTCCTGCTTGTC
GCATTGCGTGATGAAAGACT

Downstream 100 bases:

>100_bases
GAAAATTTCAGAAGCGGCGTTCGCCGCCGACCCAGACCTCGCGCACCATGAATTCGCGGTCCATCACCACGAAATCGGCC
CGCAGGCCGGGCAGCAGCGC

Product: glucose-resistance amylase regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 360; Mature: 360

Protein sequence:

>360_residues
MLAGQNLYTACTIGNDTIMPRERTPRSPISSIDVARLAGVSQSAVSRSFSPGGSVSPAMREKVLAAAAVLGYRPNLIPRI
MLSGRSRMIGVVVGGLDNPFYAAVLDHFAVVLRAVGLQVLLVQVPDALALDGALDQLAGYRVDAVVTALAVGTRQAATAL
SDLRIPIVCFNSRLTAPWISTVRSDNRAAGQAAAHLLADRGARRTAWLAGPRDSAATRARARGFLDGLAARALPAPVVIV
GQDTYHDGYAAVRDLLRGHRPPEAFFCSNDMMACGAIDALRAHGLTVPGDVRVVGYDNIPQAAWHGYDLTSFDQQVDRMA
EAALDLLTSALADPATAAKGRTRTVPARLVERGSTAPQAR

Sequences:

>Translated_360_residues
MLAGQNLYTACTIGNDTIMPRERTPRSPISSIDVARLAGVSQSAVSRSFSPGGSVSPAMREKVLAAAAVLGYRPNLIPRI
MLSGRSRMIGVVVGGLDNPFYAAVLDHFAVVLRAVGLQVLLVQVPDALALDGALDQLAGYRVDAVVTALAVGTRQAATAL
SDLRIPIVCFNSRLTAPWISTVRSDNRAAGQAAAHLLADRGARRTAWLAGPRDSAATRARARGFLDGLAARALPAPVVIV
GQDTYHDGYAAVRDLLRGHRPPEAFFCSNDMMACGAIDALRAHGLTVPGDVRVVGYDNIPQAAWHGYDLTSFDQQVDRMA
EAALDLLTSALADPATAAKGRTRTVPARLVERGSTAPQAR
>Mature_360_residues
MLAGQNLYTACTIGNDTIMPRERTPRSPISSIDVARLAGVSQSAVSRSFSPGGSVSPAMREKVLAAAAVLGYRPNLIPRI
MLSGRSRMIGVVVGGLDNPFYAAVLDHFAVVLRAVGLQVLLVQVPDALALDGALDQLAGYRVDAVVTALAVGTRQAATAL
SDLRIPIVCFNSRLTAPWISTVRSDNRAAGQAAAHLLADRGARRTAWLAGPRDSAATRARARGFLDGLAARALPAPVVIV
GQDTYHDGYAAVRDLLRGHRPPEAFFCSNDMMACGAIDALRAHGLTVPGDVRVVGYDNIPQAAWHGYDLTSFDQQVDRMA
EAALDLLTSALADPATAAKGRTRTVPARLVERGSTAPQAR

Specific function: Transcriptional repressor for the ribose rbsDACBK operon. RbsR binds to a region of perfect dyad symmetry spanning the rbs operon transcriptional start site. The affinity for the rbs operator is reduced by addition of ribose, consistent with ribose being

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790194, Length=328, Percent_Identity=31.4024390243902, Blast_Score=131, Evalue=5e-32,
Organism=Escherichia coli, GI1790369, Length=333, Percent_Identity=28.2282282282282, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI1787948, Length=329, Percent_Identity=27.0516717325228, Blast_Score=108, Evalue=4e-25,
Organism=Escherichia coli, GI1789068, Length=279, Percent_Identity=30.1075268817204, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI48994940, Length=279, Percent_Identity=28.673835125448, Blast_Score=96, Evalue=5e-21,
Organism=Escherichia coli, GI1786540, Length=333, Percent_Identity=28.2282282282282, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1788474, Length=299, Percent_Identity=28.7625418060201, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1789202, Length=270, Percent_Identity=28.5185185185185, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1790715, Length=270, Percent_Identity=24.0740740740741, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1787906, Length=254, Percent_Identity=32.2834645669291, Blast_Score=86, Evalue=5e-18,
Organism=Escherichia coli, GI1787580, Length=278, Percent_Identity=26.978417266187, Blast_Score=85, Evalue=7e-18,
Organism=Escherichia coli, GI1786268, Length=329, Percent_Identity=23.4042553191489, Blast_Score=65, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 37977; Mature: 37977

Theoretical pI: Translated: 9.86; Mature: 9.86

Prosite motif: PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLAGQNLYTACTIGNDTIMPRERTPRSPISSIDVARLAGVSQSAVSRSFSPGGSVSPAMR
CCCCCCEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHCCCHHHHHCCCCCCCCCCHHHH
EKVLAAAAVLGYRPNLIPRIMLSGRSRMIGVVVGGLDNPFYAAVLDHFAVVLRAVGLQVL
HHHHHHHHHHCCCCCHHHHHHHCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHEE
LVQVPDALALDGALDQLAGYRVDAVVTALAVGTRQAATALSDLRIPIVCFNSRLTAPWIS
EEECCCHHHHHHHHHHHHCCHHHHHHHHHHHCCHHHHHHHHHCCEEEEEECCCCCHHHHH
TVRSDNRAAGQAAAHLLADRGARRTAWLAGPRDSAATRARARGFLDGLAARALPAPVVIV
HHHCCCHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEE
GQDTYHDGYAAVRDLLRGHRPPEAFFCSNDMMACGAIDALRAHGLTVPGDVRVVGYDNIP
CCCCCCCHHHHHHHHHHCCCCCCCEEECCCCHHHHHHHHHHHCCCCCCCCEEEEECCCCC
QAAWHGYDLTSFDQQVDRMAEAALDLLTSALADPATAAKGRTRTVPARLVERGSTAPQAR
HHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MLAGQNLYTACTIGNDTIMPRERTPRSPISSIDVARLAGVSQSAVSRSFSPGGSVSPAMR
CCCCCCEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHCCCHHHHHCCCCCCCCCCHHHH
EKVLAAAAVLGYRPNLIPRIMLSGRSRMIGVVVGGLDNPFYAAVLDHFAVVLRAVGLQVL
HHHHHHHHHHCCCCCHHHHHHHCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHEE
LVQVPDALALDGALDQLAGYRVDAVVTALAVGTRQAATALSDLRIPIVCFNSRLTAPWIS
EEECCCHHHHHHHHHHHHCCHHHHHHHHHHHCCHHHHHHHHHCCEEEEEECCCCCHHHHH
TVRSDNRAAGQAAAHLLADRGARRTAWLAGPRDSAATRARARGFLDGLAARALPAPVVIV
HHHCCCHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEE
GQDTYHDGYAAVRDLLRGHRPPEAFFCSNDMMACGAIDALRAHGLTVPGDVRVVGYDNIP
CCCCCCCHHHHHHHHHHCCCCCCCEEECCCCHHHHHHHHHHHCCCCCCCCEEEEECCCCC
QAAWHGYDLTSFDQQVDRMAEAALDLLTSALADPATAAKGRTRTVPARLVERGSTAPQAR
HHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]