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The map label for this gene is metE [H]

Identifier: 162148355

GI number: 162148355

Start: 2665882

End: 2668239

Strand: Reverse

Name: metE [H]

Synonym: GDI_2572

Alternate gene names: 162148355

Gene position: 2668239-2665882 (Counterclockwise)

Preceding gene: 162148360

Following gene: 162148354

Centisome position: 67.65

GC content: 69.64

Gene sequence:

>2358_bases
ATGACCGTTACCGTCGCCAAGATTGAAGTCGCCACCCTGGGCTTTCCCCGCATCGGCCCCCGGCGTGAACTGAAGGCGGA
GCTGGAAAACCACTGGGCCGGCCGCACCGATGCCGCGTCGCTTCACGCCGCCGCAGCCCGGCTGCGTGCCGCCAACTGGG
CGTGGCAGCGCGACCGGGGCGCGGATGTGATCCCGTCCAACGATTTTTCGCTCTACGACCATGTGCTGGATACGACCGTG
ATGGTCGGGGCGATTCCGCCGGCCTATGGCTGGACCGGCGGCACGGTCGACAGCGCGACCTATTTCGCCCTGGCGCGCGG
CACCCAGGCGACGCCGGACGGGCAGGCGGACACATGCGCCCACGGCCATGCGCATGCCCACGGCGCGGGCGTGCCCGCTT
CCGAAATGACCAAATGGTTCGACACCAACTACCATTACCTGGTGCCCGAATTCTCGGCCGACCAGGTGTTCCGCCTGTCC
TCGACCAAGCCGGTCGATGAGTATCGCGAGGCCCGCGCCCAGGGAATCGAGACCCGGCCCGTCCTGCTGGGGCCGGTCAG
CTACCTGCTGCTGGGCAAGGCGCGCGGCGGCGGCTTCGATCCACTGACGCTGCTGCCGCGCCTGCTGCCGGTCTATGTCG
CGATCCTGCGGGCGCTGGCCGATGCCGGGGCGGAATGGGTGCAGATCGACGAACCCTGCCTGGTGCTGGATCTGGACGAC
GCGGCGCGGGCGGCCTTCGTCCAGGCCTATGCGCAGCTCGGCGCGGGGGCGGGCGAGGTGCGGCTGATGCTTGCGACCTA
TTTCGGGGCGCTGGGCGACAATCTGGCGACGGCGCTGTCGCTGCCGGTTCACGGTCTGCATGTCGATCTGGTCCGCGCGC
CGGAACAACTGGACGCGGTTCTGGCGGGCGCGCGGCCGGACCTGGTGCTGTCGCTGGGCGTGATCGACGGGCGCAATGTC
TGGCGGGCCGACCTGAACGCCATCCTGGCGCGGATCGCCCCGGCGGTGCGTGCGGGACGGACGCTCCAGCTTGCGCCGTC
CTGTTCGCTGTTGCATGCGCCGCTGGACCTGGACCGCGAGACGGCGCTGGAGACGGATGTCCGGTCCTGGCTGGCCTTCG
CGGTGCAGAAGATCGCCGAACTGGCCGTGCTGGCGCGCGCGTTGAACGAGGGCGAGGCCACCGTGCGCGAGGCGCTGGAT
GCGTCATCCGCCGCCGTCGCGTCGCGCCGGACCTCGCCGCGTATCAACGATCCTGTCGTCCAGGCGCGGCTGGCCGCGGA
AGGGCCGACCGCGGCCCATCGTGCATCGGCCTTTCCCGTCCGCCGGGCGGCGCAGCGCGCGCGGCTGAAGCTGCCCGCCT
ATCCCACCACCACGATCGGGTCCTTCCCGCAGACCCCGGAAATCCGCCAGGCCCGCGCCGCCCATGCCAGGGGCGAGATG
CCGCGCGCGGACTACGAGGCCTTCCTGCATGCCGAGATCGCCCGCGCCGTCGCCTGGCAGGAGGAGGCCGGACTGGACGT
GCTGGTCCATGGCGAATTCGAACGCAACGACATGGTGCAGTATTTCGGCGAACGGCTGTCGGGCTTCGCCTTCACCCGTC
ATGCCTGGGTCCAGTCCTATGGATCGCGCTATGTCCGGCCGCCGATCATCTATGGGGACGTGTCGCGGCCCGAGGCGATG
ACCGTGGCATGGTGGCGCCATGCCCAGTCGCTGACCGCCCGCCCGATGAAGGGCATGCTGACCGGTCCGGTCACGATCCT
GAACTGGTCCTTCGTCCGCGACGACCAGCCGCGCGAGACCACGTGCCGCCAGATCGCCTACGCCATCCGGGACGAGGTCG
TGGACCTGGAACGGGCGGGCGCCGCCATCATCCAGATCGACGAGGCCGCGCTGCGCGAGGGGCTGCCGTTGCGGCGGGGG
GAATGGCAGGCCTATCTGGACTGGGCGGTGGCTTGCTTCCGCATCGCCGCGTCCGGGGTGGCGGATGTGACGCAGATCCA
TACCCACATGTGCTATTCCGAGTTCAATGACATCATCGCCGCCATCGGGGCGATGGATGCCGATGTCATCTCGATCGAGA
CGGCACGCTCGAAGATGGAACTGCTCGATGCCTTCGTCGGGCATCGCTATCCGGCCGAAATCGGACCCGGCGTCTACGAC
ATCCATTCCCCGCGCGTACCCACGGTGGCCGAAATGACGGCGCTGCTGGCGGCGGCGGCCGCGCGGATCGATGCCGACCA
GATCTGGGTGAATCCCGATTGCGGTCTCAAGACCCGGAAATGGCCCGAGGTCCGCGCGGCGATCGCCGGGATGGTGGAGG
CCGCGCGCCGGATGCGGGCGGGCGACGCGACCGGCTGA

Upstream 100 bases:

>100_bases
GGTGTCCGGACGGGTCGTGACGGCACCGTGCCCGGGATGATCCGGCGCGGCTGGTGACGCGTGCCTGTTCCCCGGTCCCG
CCCAACCTGACGAGGCTGTC

Downstream 100 bases:

>100_bases
CCCCGGCCTGTTGCGTCCGGACGCGGAACGATGTTGGTTGGGCGAATCCGCGTGCGATTCGCACCCACCCCCGCCCGACT
GCCGGAGAAGCCCAGACGTG

Product: 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase

Products: NA

Alternate protein names: Cobalamin-independent methionine synthase; Methionine synthase, vitamin-B12 independent isozyme [H]

Number of amino acids: Translated: 785; Mature: 784

Protein sequence:

>785_residues
MTVTVAKIEVATLGFPRIGPRRELKAELENHWAGRTDAASLHAAAARLRAANWAWQRDRGADVIPSNDFSLYDHVLDTTV
MVGAIPPAYGWTGGTVDSATYFALARGTQATPDGQADTCAHGHAHAHGAGVPASEMTKWFDTNYHYLVPEFSADQVFRLS
STKPVDEYREARAQGIETRPVLLGPVSYLLLGKARGGGFDPLTLLPRLLPVYVAILRALADAGAEWVQIDEPCLVLDLDD
AARAAFVQAYAQLGAGAGEVRLMLATYFGALGDNLATALSLPVHGLHVDLVRAPEQLDAVLAGARPDLVLSLGVIDGRNV
WRADLNAILARIAPAVRAGRTLQLAPSCSLLHAPLDLDRETALETDVRSWLAFAVQKIAELAVLARALNEGEATVREALD
ASSAAVASRRTSPRINDPVVQARLAAEGPTAAHRASAFPVRRAAQRARLKLPAYPTTTIGSFPQTPEIRQARAAHARGEM
PRADYEAFLHAEIARAVAWQEEAGLDVLVHGEFERNDMVQYFGERLSGFAFTRHAWVQSYGSRYVRPPIIYGDVSRPEAM
TVAWWRHAQSLTARPMKGMLTGPVTILNWSFVRDDQPRETTCRQIAYAIRDEVVDLERAGAAIIQIDEAALREGLPLRRG
EWQAYLDWAVACFRIAASGVADVTQIHTHMCYSEFNDIIAAIGAMDADVISIETARSKMELLDAFVGHRYPAEIGPGVYD
IHSPRVPTVAEMTALLAAAAARIDADQIWVNPDCGLKTRKWPEVRAAIAGMVEAARRMRAGDATG

Sequences:

>Translated_785_residues
MTVTVAKIEVATLGFPRIGPRRELKAELENHWAGRTDAASLHAAAARLRAANWAWQRDRGADVIPSNDFSLYDHVLDTTV
MVGAIPPAYGWTGGTVDSATYFALARGTQATPDGQADTCAHGHAHAHGAGVPASEMTKWFDTNYHYLVPEFSADQVFRLS
STKPVDEYREARAQGIETRPVLLGPVSYLLLGKARGGGFDPLTLLPRLLPVYVAILRALADAGAEWVQIDEPCLVLDLDD
AARAAFVQAYAQLGAGAGEVRLMLATYFGALGDNLATALSLPVHGLHVDLVRAPEQLDAVLAGARPDLVLSLGVIDGRNV
WRADLNAILARIAPAVRAGRTLQLAPSCSLLHAPLDLDRETALETDVRSWLAFAVQKIAELAVLARALNEGEATVREALD
ASSAAVASRRTSPRINDPVVQARLAAEGPTAAHRASAFPVRRAAQRARLKLPAYPTTTIGSFPQTPEIRQARAAHARGEM
PRADYEAFLHAEIARAVAWQEEAGLDVLVHGEFERNDMVQYFGERLSGFAFTRHAWVQSYGSRYVRPPIIYGDVSRPEAM
TVAWWRHAQSLTARPMKGMLTGPVTILNWSFVRDDQPRETTCRQIAYAIRDEVVDLERAGAAIIQIDEAALREGLPLRRG
EWQAYLDWAVACFRIAASGVADVTQIHTHMCYSEFNDIIAAIGAMDADVISIETARSKMELLDAFVGHRYPAEIGPGVYD
IHSPRVPTVAEMTALLAAAAARIDADQIWVNPDCGLKTRKWPEVRAAIAGMVEAARRMRAGDATG
>Mature_784_residues
TVTVAKIEVATLGFPRIGPRRELKAELENHWAGRTDAASLHAAAARLRAANWAWQRDRGADVIPSNDFSLYDHVLDTTVM
VGAIPPAYGWTGGTVDSATYFALARGTQATPDGQADTCAHGHAHAHGAGVPASEMTKWFDTNYHYLVPEFSADQVFRLSS
TKPVDEYREARAQGIETRPVLLGPVSYLLLGKARGGGFDPLTLLPRLLPVYVAILRALADAGAEWVQIDEPCLVLDLDDA
ARAAFVQAYAQLGAGAGEVRLMLATYFGALGDNLATALSLPVHGLHVDLVRAPEQLDAVLAGARPDLVLSLGVIDGRNVW
RADLNAILARIAPAVRAGRTLQLAPSCSLLHAPLDLDRETALETDVRSWLAFAVQKIAELAVLARALNEGEATVREALDA
SSAAVASRRTSPRINDPVVQARLAAEGPTAAHRASAFPVRRAAQRARLKLPAYPTTTIGSFPQTPEIRQARAAHARGEMP
RADYEAFLHAEIARAVAWQEEAGLDVLVHGEFERNDMVQYFGERLSGFAFTRHAWVQSYGSRYVRPPIIYGDVSRPEAMT
VAWWRHAQSLTARPMKGMLTGPVTILNWSFVRDDQPRETTCRQIAYAIRDEVVDLERAGAAIIQIDEAALREGLPLRRGE
WQAYLDWAVACFRIAASGVADVTQIHTHMCYSEFNDIIAAIGAMDADVISIETARSKMELLDAFVGHRYPAEIGPGVYDI
HSPRVPTVAEMTALLAAAAARIDADQIWVNPDCGLKTRKWPEVRAAIAGMVEAARRMRAGDATG

Specific function: Catalyzes the transfer of a methyl group from 5- methyltetrahydrofolate to homocysteine resulting in methionine formation [H]

COG id: COG0620

COG function: function code E; Methionine synthase II (cobalamin-independent)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the vitamin-B12 independent methionine synthase family [H]

Homologues:

Organism=Escherichia coli, GI2367304, Length=770, Percent_Identity=54.2857142857143, Blast_Score=803, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6320936, Length=782, Percent_Identity=48.2097186700767, Blast_Score=716, Evalue=0.0,

Paralogues:

None

Copy number: 45,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013215
- InterPro:   IPR006276
- InterPro:   IPR002629 [H]

Pfam domain/function: PF08267 Meth_synt_1; PF01717 Meth_synt_2 [H]

EC number: =2.1.1.14 [H]

Molecular weight: Translated: 85306; Mature: 85175

Theoretical pI: Translated: 6.40; Mature: 6.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVTVAKIEVATLGFPRIGPRRELKAELENHWAGRTDAASLHAAAARLRAANWAWQRDRG
CEEEEEEEEEEECCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCC
ADVIPSNDFSLYDHVLDTTVMVGAIPPAYGWTGGTVDSATYFALARGTQATPDGQADTCA
CCCCCCCCHHHHHHHHHHHHHEECCCCCCCCCCCCCCCHHEEEEECCCCCCCCCCCCCCC
HGHAHAHGAGVPASEMTKWFDTNYHYLVPEFSADQVFRLSSTKPVDEYREARAQGIETRP
CCCCCCCCCCCCHHHHHHHHCCCCEEECCCCCCCCEEEECCCCCHHHHHHHHHCCCCCCC
VLLGPVSYLLLGKARGGGFDPLTLLPRLLPVYVAILRALADAGAEWVQIDEPCLVLDLDD
EEECCHHHHHEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCEEEEECCH
AARAAFVQAYAQLGAGAGEVRLMLATYFGALGDNLATALSLPVHGLHVDLVRAPEQLDAV
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHCCHHHHHHH
LAGARPDLVLSLGVIDGRNVWRADLNAILARIAPAVRAGRTLQLAPSCSLLHAPLDLDRE
HCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCEEEECCCCCEEECCCCCCHH
TALETDVRSWLAFAVQKIAELAVLARALNEGEATVREALDASSAAVASRRTSPRINDPVV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHCCCCCCCCCHHH
QARLAAEGPTAAHRASAFPVRRAAQRARLKLPAYPTTTIGSFPQTPEIRQARAAHARGEM
HHHHHCCCCCHHHHHCCCHHHHHHHHHCEECCCCCCCCCCCCCCCCHHHHHHHHHHCCCC
PRADYEAFLHAEIARAVAWQEEAGLDVLVHGEFERNDMVQYFGERLSGFAFTRHAWVQSY
CCHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHCCHHHHHHHHHHHH
GSRYVRPPIIYGDVSRPEAMTVAWWRHAQSLTARPMKGMLTGPVTILNWSFVRDDQPRET
CCCCCCCCEEECCCCCCCCEEHHHHHHHHHHHCCCCCCCCCCCEEEEEEEECCCCCCHHH
TCRQIAYAIRDEVVDLERAGAAIIQIDEAALREGLPLRRGEWQAYLDWAVACFRIAASGV
HHHHHHHHHHHHHHHHHHCCEEEEEECHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCH
ADVTQIHTHMCYSEFNDIIAAIGAMDADVISIETARSKMELLDAFVGHRYPAEIGPGVYD
HHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHCCCCCHHHCCCEEE
IHSPRVPTVAEMTALLAAAAARIDADQIWVNPDCGLKTRKWPEVRAAIAGMVEAARRMRA
CCCCCCCHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHCC
GDATG
CCCCC
>Mature Secondary Structure 
TVTVAKIEVATLGFPRIGPRRELKAELENHWAGRTDAASLHAAAARLRAANWAWQRDRG
EEEEEEEEEEECCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCC
ADVIPSNDFSLYDHVLDTTVMVGAIPPAYGWTGGTVDSATYFALARGTQATPDGQADTCA
CCCCCCCCHHHHHHHHHHHHHEECCCCCCCCCCCCCCCHHEEEEECCCCCCCCCCCCCCC
HGHAHAHGAGVPASEMTKWFDTNYHYLVPEFSADQVFRLSSTKPVDEYREARAQGIETRP
CCCCCCCCCCCCHHHHHHHHCCCCEEECCCCCCCCEEEECCCCCHHHHHHHHHCCCCCCC
VLLGPVSYLLLGKARGGGFDPLTLLPRLLPVYVAILRALADAGAEWVQIDEPCLVLDLDD
EEECCHHHHHEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCEEEEECCH
AARAAFVQAYAQLGAGAGEVRLMLATYFGALGDNLATALSLPVHGLHVDLVRAPEQLDAV
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHCCHHHHHHH
LAGARPDLVLSLGVIDGRNVWRADLNAILARIAPAVRAGRTLQLAPSCSLLHAPLDLDRE
HCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCEEEECCCCCEEECCCCCCHH
TALETDVRSWLAFAVQKIAELAVLARALNEGEATVREALDASSAAVASRRTSPRINDPVV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHCCCCCCCCCHHH
QARLAAEGPTAAHRASAFPVRRAAQRARLKLPAYPTTTIGSFPQTPEIRQARAAHARGEM
HHHHHCCCCCHHHHHCCCHHHHHHHHHCEECCCCCCCCCCCCCCCCHHHHHHHHHHCCCC
PRADYEAFLHAEIARAVAWQEEAGLDVLVHGEFERNDMVQYFGERLSGFAFTRHAWVQSY
CCHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHCCHHHHHHHHHHHH
GSRYVRPPIIYGDVSRPEAMTVAWWRHAQSLTARPMKGMLTGPVTILNWSFVRDDQPRET
CCCCCCCCEEECCCCCCCCEEHHHHHHHHHHHCCCCCCCCCCCEEEEEEEECCCCCCHHH
TCRQIAYAIRDEVVDLERAGAAIIQIDEAALREGLPLRRGEWQAYLDWAVACFRIAASGV
HHHHHHHHHHHHHHHHHHCCEEEEEECHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCH
ADVTQIHTHMCYSEFNDIIAAIGAMDADVISIETARSKMELLDAFVGHRYPAEIGPGVYD
HHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHCCCCCHHHCCCEEE
IHSPRVPTVAEMTALLAAAAARIDADQIWVNPDCGLKTRKWPEVRAAIAGMVEAARRMRA
CCCCCCCHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHCC
GDATG
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11259647 [H]