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The map label for this gene is gumD [H]

Identifier: 162148330

GI number: 162148330

Start: 2643476

End: 2644996

Strand: Direct

Name: gumD [H]

Synonym: GDI_2547

Alternate gene names: 162148330

Gene position: 2643476-2644996 (Clockwise)

Preceding gene: 162148328

Following gene: 162148331

Centisome position: 67.02

GC content: 64.17

Gene sequence:

>1521_bases
TTGCCCGATGGAGATCTTCGCATGAGCGAGTGGTATGGCCAGTTGCAGGATTCGATCGGTCTTTCGACCGATCCGATCAC
ATACAAGACGCCTTCGGCCAGGATCGCCGAACCCGGGACCTATCCTTATTATCATTCCGTTCTGGCCGGGATGATAACCA
GCGTAGATACCCTGGCGATTCTGGCCGCCACGTTCATCGGCGCCTCCGTCGAACCGGCGATCATCCACCAGGACTGGCGG
GTGGCGCAGCCGCTGGCGGACCTGATGTCGGCGCTGGGGTTCCTGATGCTCCCCAAGAACCGCCGTTTGCTTCATGTCCC
TACGGTACAGGATTTTTCGGCACAGGTCCGCTATCTGACGCCGCCGCTTCTGGCCGGGGCGCTGCTGCACGTCATCGTCC
TGTACATGCTGCATCATCCCATCGTGCCGTCGTTCGAACTGGCGCTGACGTGGCTGGCTTTTTCGACCGGCGTGCTGGCG
GTGGTGCGCGGAACCGAAACGGTGCTGCTGTCCCGGCCGGCAATCGCAAACCAGCTGGCACGCAACGTGGCCGTGGTCGG
CAGCGACGACGCCGCCATCAGGCTGGCCGCCCGCATCGGGGAGGAAGCCGGATCGACCTATCGCATGATCGGCGTATTCG
ACGACCATGACACCGCGCTGGACCCCACCGCCACCACCGGCACGCTGGACGACCTGATCGAGCGCAGCCGCGAAACCCCG
CTGCATGCGATCATTCTGGCCATTCCCCCCAGTACCGATCCGATCGACCATGTAGCCGAAATCAACTGGCGGCTGCGCAG
CGTTCTGGCGGACGTCTATGTGCTGCCCAATATCGTGCATGGCATCGACGTCCTGCTGCCGATCGAACGGCTGGGGCCGT
TCGCGCTGCTGGTCCTTCAGCGTCGCCCGCTGTCGGACTGGCAGATCGTCAAGAAGACGGTCCTGGACGTCTTCCTGGGC
GCAATCGCCCTGGTGATGCTCGCGCCGCTGATGGCGGCGGTCGCGATCACGATCAAGGCGACCTCGCCCGGTCCCGTCTT
CTTCCGGCAGCCGCGCCTGGGGTTCAACAACCGGACCTTCATGGTCTTCAAGTTCCGCACGATGTTCACCGACAAGTCGG
ACATGATGGCCGCGCGCCAGACCGCGCGGGACGATCCGCGCGTGACCCCCATCGGCAAGTGGCTGCGCAAGCTGAGCATC
GACGAGCTGCCGCAATTGCTGAACGTTCTTCGCGGCGAGATGTCGCTGGTCGGTCCCCGCCCGCACGCGCCGCACACCCG
CGCGGCCGGCATGCTGCTGGACGATGCGCTGGCGGAATATGTCATCCGCCACCAGGTCAAGCCCGGCATTACCGGATGGG
CGCAGGTCAACGGCGCGCGCGGCCAGTTGGTCACGCTGGACGATCTGCGCCGCCGCGTCGAACTGGACCTGGAATACATG
CAGAGATGGTCGCTGCGCTTCGATCTGAAGATCCTGATGCTGACCGTGGTGCGAGAGGTCTTCAGCCGTCATGCCTTCTG
A

Upstream 100 bases:

>100_bases
CCTGATTGAGAAATTCGTGAACTTTTTGACACACTCGTCTTAAAAAGACTTTTTTCAGATCATAACATTTCGAAACGGTA
AAACGGTCGACAGTGAGCGC

Downstream 100 bases:

>100_bases
CGCCATCGCTCCCTTCGTCCCTGCCCCCCTCACCCCGACGCAGGCCGACGAACTGCGCGTAATGGGGCTGCGCCTGCTGG
ACATTCCTCGCCAGGACCTG

Product: polysaccharide biosynthesis glycosyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 506; Mature: 505

Protein sequence:

>506_residues
MPDGDLRMSEWYGQLQDSIGLSTDPITYKTPSARIAEPGTYPYYHSVLAGMITSVDTLAILAATFIGASVEPAIIHQDWR
VAQPLADLMSALGFLMLPKNRRLLHVPTVQDFSAQVRYLTPPLLAGALLHVIVLYMLHHPIVPSFELALTWLAFSTGVLA
VVRGTETVLLSRPAIANQLARNVAVVGSDDAAIRLAARIGEEAGSTYRMIGVFDDHDTALDPTATTGTLDDLIERSRETP
LHAIILAIPPSTDPIDHVAEINWRLRSVLADVYVLPNIVHGIDVLLPIERLGPFALLVLQRRPLSDWQIVKKTVLDVFLG
AIALVMLAPLMAAVAITIKATSPGPVFFRQPRLGFNNRTFMVFKFRTMFTDKSDMMAARQTARDDPRVTPIGKWLRKLSI
DELPQLLNVLRGEMSLVGPRPHAPHTRAAGMLLDDALAEYVIRHQVKPGITGWAQVNGARGQLVTLDDLRRRVELDLEYM
QRWSLRFDLKILMLTVVREVFSRHAF

Sequences:

>Translated_506_residues
MPDGDLRMSEWYGQLQDSIGLSTDPITYKTPSARIAEPGTYPYYHSVLAGMITSVDTLAILAATFIGASVEPAIIHQDWR
VAQPLADLMSALGFLMLPKNRRLLHVPTVQDFSAQVRYLTPPLLAGALLHVIVLYMLHHPIVPSFELALTWLAFSTGVLA
VVRGTETVLLSRPAIANQLARNVAVVGSDDAAIRLAARIGEEAGSTYRMIGVFDDHDTALDPTATTGTLDDLIERSRETP
LHAIILAIPPSTDPIDHVAEINWRLRSVLADVYVLPNIVHGIDVLLPIERLGPFALLVLQRRPLSDWQIVKKTVLDVFLG
AIALVMLAPLMAAVAITIKATSPGPVFFRQPRLGFNNRTFMVFKFRTMFTDKSDMMAARQTARDDPRVTPIGKWLRKLSI
DELPQLLNVLRGEMSLVGPRPHAPHTRAAGMLLDDALAEYVIRHQVKPGITGWAQVNGARGQLVTLDDLRRRVELDLEYM
QRWSLRFDLKILMLTVVREVFSRHAF
>Mature_505_residues
PDGDLRMSEWYGQLQDSIGLSTDPITYKTPSARIAEPGTYPYYHSVLAGMITSVDTLAILAATFIGASVEPAIIHQDWRV
AQPLADLMSALGFLMLPKNRRLLHVPTVQDFSAQVRYLTPPLLAGALLHVIVLYMLHHPIVPSFELALTWLAFSTGVLAV
VRGTETVLLSRPAIANQLARNVAVVGSDDAAIRLAARIGEEAGSTYRMIGVFDDHDTALDPTATTGTLDDLIERSRETPL
HAIILAIPPSTDPIDHVAEINWRLRSVLADVYVLPNIVHGIDVLLPIERLGPFALLVLQRRPLSDWQIVKKTVLDVFLGA
IALVMLAPLMAAVAITIKATSPGPVFFRQPRLGFNNRTFMVFKFRTMFTDKSDMMAARQTARDDPRVTPIGKWLRKLSID
ELPQLLNVLRGEMSLVGPRPHAPHTRAAGMLLDDALAEYVIRHQVKPGITGWAQVNGARGQLVTLDDLRRRVELDLEYMQ
RWSLRFDLKILMLTVVREVFSRHAF

Specific function: Slime polysaccharide colanic acid biosynthesis. [C]

COG id: COG2148

COG function: function code M; Sugar transferases involved in lipopolysaccharide synthesis

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial sugar transferase family [H]

Homologues:

Organism=Escherichia coli, GI1788360, Length=369, Percent_Identity=33.3333333333333, Blast_Score=209, Evalue=4e-55,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003362
- InterPro:   IPR017475
- InterPro:   IPR017473 [H]

Pfam domain/function: PF02397 Bac_transf [H]

EC number: NA

Molecular weight: Translated: 56203; Mature: 56072

Theoretical pI: Translated: 8.08; Mature: 8.08

Prosite motif: PS01034 GLYCOSYL_HYDROL_F16

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPDGDLRMSEWYGQLQDSIGLSTDPITYKTPSARIAEPGTYPYYHSVLAGMITSVDTLAI
CCCCCCCHHHHHHHHHHHCCCCCCCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
LAATFIGASVEPAIIHQDWRVAQPLADLMSALGFLMLPKNRRLLHVPTVQDFSAQVRYLT
HHHHHHCCCCCCHHEECCCHHHHHHHHHHHHHHHEECCCCCCEEECCCCCCCCCCEEECC
PPLLAGALLHVIVLYMLHHPIVPSFELALTWLAFSTGVLAVVRGTETVLLSRPAIANQLA
CHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCEEEEECCCEEEEECCHHHHHHH
RNVAVVGSDDAAIRLAARIGEEAGSTYRMIGVFDDHDTALDPTATTGTLDDLIERSRETP
HCEEEECCCCHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCCCCCCHHHHHHHCCCCC
LHAIILAIPPSTDPIDHVAEINWRLRSVLADVYVLPNIVHGIDVLLPIERLGPFALLVLQ
EEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHEEEEC
RRPLSDWQIVKKTVLDVFLGAIALVMLAPLMAAVAITIKATSPGPVFFRQPRLGFNNRTF
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCEEEECCCCCCCCCEE
MVFKFRTMFTDKSDMMAARQTARDDPRVTPIGKWLRKLSIDELPQLLNVLRGEMSLVGPR
EEEEEEEHHCCCHHHHHHHHHCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHCHHHCCCCC
PHAPHTRAAGMLLDDALAEYVIRHQVKPGITGWAQVNGARGQLVTLDDLRRRVELDLEYM
CCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCCCEEEHHHHHHHHHHHHHHH
QRWSLRFDLKILMLTVVREVFSRHAF
HHCCCHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
PDGDLRMSEWYGQLQDSIGLSTDPITYKTPSARIAEPGTYPYYHSVLAGMITSVDTLAI
CCCCCCHHHHHHHHHHHCCCCCCCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
LAATFIGASVEPAIIHQDWRVAQPLADLMSALGFLMLPKNRRLLHVPTVQDFSAQVRYLT
HHHHHHCCCCCCHHEECCCHHHHHHHHHHHHHHHEECCCCCCEEECCCCCCCCCCEEECC
PPLLAGALLHVIVLYMLHHPIVPSFELALTWLAFSTGVLAVVRGTETVLLSRPAIANQLA
CHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCEEEEECCCEEEEECCHHHHHHH
RNVAVVGSDDAAIRLAARIGEEAGSTYRMIGVFDDHDTALDPTATTGTLDDLIERSRETP
HCEEEECCCCHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCCCCCCHHHHHHHCCCCC
LHAIILAIPPSTDPIDHVAEINWRLRSVLADVYVLPNIVHGIDVLLPIERLGPFALLVLQ
EEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHEEEEC
RRPLSDWQIVKKTVLDVFLGAIALVMLAPLMAAVAITIKATSPGPVFFRQPRLGFNNRTF
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCEEEECCCCCCCCCEE
MVFKFRTMFTDKSDMMAARQTARDDPRVTPIGKWLRKLSIDELPQLLNVLRGEMSLVGPR
EEEEEEEHHCCCHHHHHHHHHCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHCHHHCCCCC
PHAPHTRAAGMLLDDALAEYVIRHQVKPGITGWAQVNGARGQLVTLDDLRRRVELDLEYM
CCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCCCEEEHHHHHHHHHHHHHHH
QRWSLRFDLKILMLTVVREVFSRHAF
HHCCCHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8759852; 9097040; 9278503 [H]