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The map label for this gene is acsA [H]

Identifier: 162148229

GI number: 162148229

Start: 2535522

End: 2537459

Strand: Reverse

Name: acsA [H]

Synonym: GDI_2446

Alternate gene names: 162148229

Gene position: 2537459-2535522 (Counterclockwise)

Preceding gene: 162148230

Following gene: 162148228

Centisome position: 64.33

GC content: 70.54

Gene sequence:

>1938_bases
GTGATACACGCAGAGACCTTTCCCGTTCCACCCTCGTTCGCGGCACAGGCTGCCGTCGATGCCCCCCGCCTGCGCCAGAT
GACCGATGCCGCGTCGCAGCATCCCGATCAGTTCTGGCTGGACCAGGCCGGGCGCATCGACTGGGCGGTCCGGCCGACGA
CCGCGTCGTCCTCGACCTTCGGGGGCGACGTGGCGATACGCTGGTTCGCCGACGGCACGCTGAACGCCAGCGCCAATTGC
CTGGACCGCCACCTGGTGACGCGGGGCGACCAGGTCGCGCTGATCTGGCAGGGGCAGGATCAGGGACAGGTCGAACGCCT
GACCTACCGGGCGCTGCACCAGCGCGTGTGCCGGATGGCCAACGTCCTGCGCGGGCTGGGCGTCGGGCGGGGCGACCGGG
TGGCGATCCACCTGCCGATGGTGATCGAGGGCGTGGTCGCGATGCTGGCCTGCGCCCGGATCGGCGCGGTGCATGTCGTG
CTGTTCGGCGGGTTCTCGGCGGAAGGGCTGGCCGACCGCCTGGTCAACAGCGGGGCGGTGGTGGTGATCACCGCCGACGA
GGGACGGCGCGGCGCCAAGCGCATTCCGCTGAAGGCCACGATGGACGACGCCGTGGCCCGTGACGGCGTGCAGGTGCGCC
ACGTGCTGGTCCTGGCCGTGACCGGGGCGGCGGTACCGATGCACGCGGGCCGCGACCTGCCGCTGAACCCGCTGCTGGCG
CAGGCTGCGGAGGACTGCCCGGCCGAAATCATGGGGGCCGAGGACCCGCTGTTCGTCCTCTATACCTCGGGCAGCACCGG
CAAGCCGAAGGGCATGGTCCACACCACCGGCGGCTACATGGTCTGGGCGTCCTATACCCACGAACTGGTCTTCGATCACC
GGGCGGGCGACGTGTTCTGGTGTACGGCGGACATCAGCTGGATCACCGGCCACACCTATGTCGTCTACGGCCCGCTGGCC
AATGGCAGCACCGTCGTGCTGTTCGAGGGGCTGCCGTCCCACCCCGCTCCGGGCCGGTGGTGGGAGGTGATCGACACCCA
CAAGGTCACGACCTTCTACACCGCGCCGACCGCGATCCGCGCGCTGATGCGCGACGGCGACGACGTGGTGGACCGCTACG
CGCTGGACAGCCTGCGCCTGCTGGGCAGCGTGGGCGAACCGATCAGTGCCGATGCGTGGCTGTGGTTTCACGAGAAGGTC
GGGCGCGGACGCTGCCCGGTGGTCGATACCTGGTGGCAAACCGAAACCGGCGGCATCCTGATCGCCCCGGTGCCCGGCGC
GGTGCCGCAGAAGCCCGGGGCGGCGACCCTGCCGCTGCCGGGCGTAGCCTGCGTCCTGCTGACGGACAAGGGCGCGGTGA
TCGAGGGCGAGGGCGAGGGCTGCCTGTGCGTGGCACGATCCTGGCCCGGGCAGGCCCGGACGATCTGGGCCGACCATGCG
CGGTTCCGCCAGACCTATTTCACCTTCAGCCCCGGCTATTATTTCTCGGGCGACGGGGCGCGGCGCGACGCCGACGGCTA
TTACTGGATCACCGGGCGGATCGACGACGTCATCAACGTGTCCGGCCACCGTATCGGCACGGCCGAAATCGAGGACGTGG
TGGCCACCGACCATCGCTTCGTGGAATGCGCGGCGGTAGGCATCCCCCATGACCTGAAGGGGCAGGGACTGGTGGTGTTC
GCGGTCGCGCGCGACATGGCCGAGCCCGGCCTGGCCGAGGCCGCGGCGCTGGCCGTCACCAGGGGCGTGGGGCGCTACGC
GGTGCCGGAGCGCGTCTATCTGGTGCCCGACCTGCCGAAGACCCGGTCGGGCAAGATCGTGCGGCGGCTGCTGCGCAAGA
TCGCCTGCCACGAACTGGACGGGCTGGGCGACCTGTCGACGCTGGCCGATCCCACGATCGTGGACCGGCTGATCGCCGAG
GTTGCGCGGGTGGCGTAA

Upstream 100 bases:

>100_bases
GCTCGTCTCCCAAGGGTGAAACCGTTATATTCACCGTCTATACCCTCCCATTCAAGAAGCCGGATTCCCTACCGGCCATG
ACAGGCGAATAGAATAAACC

Downstream 100 bases:

>100_bases
TGGCGGCCTGGGGGAGGGGCGGAATGATGCGATCCGTGCGGTTTTTCGCCGTGCTGGGCCTGGTCGTGGCGGCAGTATGT
GCCCCCTCCGGCGGGGCGTG

Product: acetyl-coenzyme A synthetase

Products: NA

Alternate protein names: Acetate--CoA ligase; Acyl-activating enzyme [H]

Number of amino acids: Translated: 645; Mature: 645

Protein sequence:

>645_residues
MIHAETFPVPPSFAAQAAVDAPRLRQMTDAASQHPDQFWLDQAGRIDWAVRPTTASSSTFGGDVAIRWFADGTLNASANC
LDRHLVTRGDQVALIWQGQDQGQVERLTYRALHQRVCRMANVLRGLGVGRGDRVAIHLPMVIEGVVAMLACARIGAVHVV
LFGGFSAEGLADRLVNSGAVVVITADEGRRGAKRIPLKATMDDAVARDGVQVRHVLVLAVTGAAVPMHAGRDLPLNPLLA
QAAEDCPAEIMGAEDPLFVLYTSGSTGKPKGMVHTTGGYMVWASYTHELVFDHRAGDVFWCTADISWITGHTYVVYGPLA
NGSTVVLFEGLPSHPAPGRWWEVIDTHKVTTFYTAPTAIRALMRDGDDVVDRYALDSLRLLGSVGEPISADAWLWFHEKV
GRGRCPVVDTWWQTETGGILIAPVPGAVPQKPGAATLPLPGVACVLLTDKGAVIEGEGEGCLCVARSWPGQARTIWADHA
RFRQTYFTFSPGYYFSGDGARRDADGYYWITGRIDDVINVSGHRIGTAEIEDVVATDHRFVECAAVGIPHDLKGQGLVVF
AVARDMAEPGLAEAAALAVTRGVGRYAVPERVYLVPDLPKTRSGKIVRRLLRKIACHELDGLGDLSTLADPTIVDRLIAE
VARVA

Sequences:

>Translated_645_residues
MIHAETFPVPPSFAAQAAVDAPRLRQMTDAASQHPDQFWLDQAGRIDWAVRPTTASSSTFGGDVAIRWFADGTLNASANC
LDRHLVTRGDQVALIWQGQDQGQVERLTYRALHQRVCRMANVLRGLGVGRGDRVAIHLPMVIEGVVAMLACARIGAVHVV
LFGGFSAEGLADRLVNSGAVVVITADEGRRGAKRIPLKATMDDAVARDGVQVRHVLVLAVTGAAVPMHAGRDLPLNPLLA
QAAEDCPAEIMGAEDPLFVLYTSGSTGKPKGMVHTTGGYMVWASYTHELVFDHRAGDVFWCTADISWITGHTYVVYGPLA
NGSTVVLFEGLPSHPAPGRWWEVIDTHKVTTFYTAPTAIRALMRDGDDVVDRYALDSLRLLGSVGEPISADAWLWFHEKV
GRGRCPVVDTWWQTETGGILIAPVPGAVPQKPGAATLPLPGVACVLLTDKGAVIEGEGEGCLCVARSWPGQARTIWADHA
RFRQTYFTFSPGYYFSGDGARRDADGYYWITGRIDDVINVSGHRIGTAEIEDVVATDHRFVECAAVGIPHDLKGQGLVVF
AVARDMAEPGLAEAAALAVTRGVGRYAVPERVYLVPDLPKTRSGKIVRRLLRKIACHELDGLGDLSTLADPTIVDRLIAE
VARVA
>Mature_645_residues
MIHAETFPVPPSFAAQAAVDAPRLRQMTDAASQHPDQFWLDQAGRIDWAVRPTTASSSTFGGDVAIRWFADGTLNASANC
LDRHLVTRGDQVALIWQGQDQGQVERLTYRALHQRVCRMANVLRGLGVGRGDRVAIHLPMVIEGVVAMLACARIGAVHVV
LFGGFSAEGLADRLVNSGAVVVITADEGRRGAKRIPLKATMDDAVARDGVQVRHVLVLAVTGAAVPMHAGRDLPLNPLLA
QAAEDCPAEIMGAEDPLFVLYTSGSTGKPKGMVHTTGGYMVWASYTHELVFDHRAGDVFWCTADISWITGHTYVVYGPLA
NGSTVVLFEGLPSHPAPGRWWEVIDTHKVTTFYTAPTAIRALMRDGDDVVDRYALDSLRLLGSVGEPISADAWLWFHEKV
GRGRCPVVDTWWQTETGGILIAPVPGAVPQKPGAATLPLPGVACVLLTDKGAVIEGEGEGCLCVARSWPGQARTIWADHA
RFRQTYFTFSPGYYFSGDGARRDADGYYWITGRIDDVINVSGHRIGTAEIEDVVATDHRFVECAAVGIPHDLKGQGLVVF
AVARDMAEPGLAEAAALAVTRGVGRYAVPERVYLVPDLPKTRSGKIVRRLLRKIACHELDGLGDLSTLADPTIVDRLIAE
VARVA

Specific function: Enables The Cell To Use Acetate During Aerobic Growth To Generate Energy Via The Tca Cycle, And Biosynthetic Compounds Via The Glyoxylate Shunt. Acetylates Chey, The Response Regulator Involved In Flagellar Movement And Chemotaxis. [C]

COG id: COG0365

COG function: function code I; Acyl-coenzyme A synthetases/AMP-(fatty) acid ligases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI28416953, Length=580, Percent_Identity=51.8965517241379, Blast_Score=591, Evalue=1e-169,
Organism=Homo sapiens, GI8923896, Length=670, Percent_Identity=46.5671641791045, Blast_Score=590, Evalue=1e-168,
Organism=Homo sapiens, GI253970489, Length=683, Percent_Identity=45.6808199121523, Blast_Score=585, Evalue=1e-167,
Organism=Homo sapiens, GI13375727, Length=617, Percent_Identity=34.0356564019449, Blast_Score=346, Evalue=3e-95,
Organism=Homo sapiens, GI31982927, Length=567, Percent_Identity=26.4550264550265, Blast_Score=168, Evalue=1e-41,
Organism=Homo sapiens, GI42544132, Length=526, Percent_Identity=27.1863117870722, Blast_Score=166, Evalue=6e-41,
Organism=Homo sapiens, GI38505220, Length=532, Percent_Identity=27.8195488721804, Blast_Score=157, Evalue=2e-38,
Organism=Homo sapiens, GI122937307, Length=551, Percent_Identity=25.4083484573503, Blast_Score=149, Evalue=6e-36,
Organism=Homo sapiens, GI115511026, Length=542, Percent_Identity=25.2767527675277, Blast_Score=135, Evalue=1e-31,
Organism=Homo sapiens, GI58082049, Length=524, Percent_Identity=25.381679389313, Blast_Score=127, Evalue=3e-29,
Organism=Homo sapiens, GI157311624, Length=526, Percent_Identity=24.5247148288973, Blast_Score=120, Evalue=3e-27,
Organism=Homo sapiens, GI157311622, Length=526, Percent_Identity=24.5247148288973, Blast_Score=120, Evalue=3e-27,
Organism=Homo sapiens, GI42544134, Length=348, Percent_Identity=25.8620689655172, Blast_Score=96, Evalue=1e-19,
Organism=Homo sapiens, GI195947378, Length=436, Percent_Identity=22.7064220183486, Blast_Score=89, Evalue=1e-17,
Organism=Escherichia coli, GI1790505, Length=649, Percent_Identity=51.15562403698, Blast_Score=688, Evalue=0.0,
Organism=Escherichia coli, GI1786529, Length=619, Percent_Identity=38.2875605815832, Blast_Score=396, Evalue=1e-111,
Organism=Escherichia coli, GI221142682, Length=528, Percent_Identity=25, Blast_Score=127, Evalue=2e-30,
Organism=Escherichia coli, GI1788107, Length=573, Percent_Identity=25.130890052356, Blast_Score=105, Evalue=9e-24,
Organism=Escherichia coli, GI145693145, Length=521, Percent_Identity=24.5681381957774, Blast_Score=96, Evalue=9e-21,
Organism=Caenorhabditis elegans, GI71983001, Length=653, Percent_Identity=43.7978560490046, Blast_Score=530, Evalue=1e-151,
Organism=Caenorhabditis elegans, GI71982997, Length=653, Percent_Identity=43.7978560490046, Blast_Score=530, Evalue=1e-150,
Organism=Caenorhabditis elegans, GI17569507, Length=589, Percent_Identity=22.9202037351443, Blast_Score=123, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI32563687, Length=550, Percent_Identity=22.5454545454545, Blast_Score=101, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17558820, Length=513, Percent_Identity=23.3918128654971, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17538037, Length=589, Percent_Identity=23.5993208828523, Blast_Score=85, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI115534440, Length=535, Percent_Identity=22.0560747663551, Blast_Score=70, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6323182, Length=642, Percent_Identity=46.417445482866, Blast_Score=564, Evalue=1e-161,
Organism=Saccharomyces cerevisiae, GI6319264, Length=588, Percent_Identity=47.7891156462585, Blast_Score=550, Evalue=1e-157,
Organism=Saccharomyces cerevisiae, GI6319699, Length=362, Percent_Identity=23.7569060773481, Blast_Score=96, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24667955, Length=657, Percent_Identity=46.2709284627093, Blast_Score=583, Evalue=1e-166,
Organism=Drosophila melanogaster, GI62472339, Length=550, Percent_Identity=50.3636363636364, Blast_Score=550, Evalue=1e-157,
Organism=Drosophila melanogaster, GI24667959, Length=519, Percent_Identity=50.2890173410405, Blast_Score=512, Evalue=1e-145,
Organism=Drosophila melanogaster, GI21355225, Length=607, Percent_Identity=33.1136738056013, Blast_Score=318, Evalue=8e-87,
Organism=Drosophila melanogaster, GI18859661, Length=503, Percent_Identity=25.0497017892644, Blast_Score=89, Evalue=1e-17,
Organism=Drosophila melanogaster, GI21355181, Length=576, Percent_Identity=23.0902777777778, Blast_Score=84, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24648260, Length=387, Percent_Identity=23.2558139534884, Blast_Score=72, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011904
- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.1 [H]

Molecular weight: Translated: 69543; Mature: 69543

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIHAETFPVPPSFAAQAAVDAPRLRQMTDAASQHPDQFWLDQAGRIDWAVRPTTASSSTF
CCCCCCCCCCCCHHHHHHCCCHHHHHHHHHHHCCCCHHHHCCCCCEEEEEECCCCCCCCC
GGDVAIRWFADGTLNASANCLDRHLVTRGDQVALIWQGQDQGQVERLTYRALHQRVCRMA
CCCEEEEEEECCCCCCCHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHH
NVLRGLGVGRGDRVAIHLPMVIEGVVAMLACARIGAVHVVLFGGFSAEGLADRLVNSGAV
HHHHHCCCCCCCEEEEECHHHHHHHHHHHHHHHHCEEEEEEEECCCCCHHHHHHHCCCCE
VVITADEGRRGAKRIPLKATMDDAVARDGVQVRHVLVLAVTGAAVPMHAGRDLPLNPLLA
EEEECCCCCCCCCCCCCEECHHHHHHHCCCEEEEEEEEEEECCEEECCCCCCCCCCHHHH
QAAEDCPAEIMGAEDPLFVLYTSGSTGKPKGMVHTTGGYMVWASYTHELVFDHRAGDVFW
HHHHCCCHHHCCCCCCEEEEEECCCCCCCCCEEEECCCEEEEEECCEEEEEECCCCCEEE
CTADISWITGHTYVVYGPLANGSTVVLFEGLPSHPAPGRWWEVIDTHKVTTFYTAPTAIR
EEECCEEEECCEEEEEEECCCCCEEEEECCCCCCCCCCCCEEEEECCEEEEEEECHHHHH
ALMRDGDDVVDRYALDSLRLLGSVGEPISADAWLWFHEKVGRGRCPVVDTWWQTETGGIL
HHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEHHCCCCCCCEEECCCCCCCCCEE
IAPVPGAVPQKPGAATLPLPGVACVLLTDKGAVIEGEGEGCLCVARSWPGQARTIWADHA
EECCCCCCCCCCCCCCCCCCCEEEEEEECCCCEEECCCCCEEEEEECCCCCCEEEEHHHH
RFRQTYFTFSPGYYFSGDGARRDADGYYWITGRIDDVINVSGHRIGTAEIEDVVATDHRF
HHEEEEEEECCCEEECCCCCCCCCCCEEEEEECCCCEEECCCCEECCHHHHHHHHCCCCE
VECAAVGIPHDLKGQGLVVFAVARDMAEPGLAEAAALAVTRGVGRYAVPERVYLVPDLPK
EEEEEECCCCCCCCCCEEEEEEEHHCCCCCHHHHHHHHHHHCCCCCCCCCEEEEECCCCC
TRSGKIVRRLLRKIACHELDGLGDLSTLADPTIVDRLIAEVARVA
CCCCHHHHHHHHHHHHHHCCCCCCHHHHCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MIHAETFPVPPSFAAQAAVDAPRLRQMTDAASQHPDQFWLDQAGRIDWAVRPTTASSSTF
CCCCCCCCCCCCHHHHHHCCCHHHHHHHHHHHCCCCHHHHCCCCCEEEEEECCCCCCCCC
GGDVAIRWFADGTLNASANCLDRHLVTRGDQVALIWQGQDQGQVERLTYRALHQRVCRMA
CCCEEEEEEECCCCCCCHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHH
NVLRGLGVGRGDRVAIHLPMVIEGVVAMLACARIGAVHVVLFGGFSAEGLADRLVNSGAV
HHHHHCCCCCCCEEEEECHHHHHHHHHHHHHHHHCEEEEEEEECCCCCHHHHHHHCCCCE
VVITADEGRRGAKRIPLKATMDDAVARDGVQVRHVLVLAVTGAAVPMHAGRDLPLNPLLA
EEEECCCCCCCCCCCCCEECHHHHHHHCCCEEEEEEEEEEECCEEECCCCCCCCCCHHHH
QAAEDCPAEIMGAEDPLFVLYTSGSTGKPKGMVHTTGGYMVWASYTHELVFDHRAGDVFW
HHHHCCCHHHCCCCCCEEEEEECCCCCCCCCEEEECCCEEEEEECCEEEEEECCCCCEEE
CTADISWITGHTYVVYGPLANGSTVVLFEGLPSHPAPGRWWEVIDTHKVTTFYTAPTAIR
EEECCEEEECCEEEEEEECCCCCEEEEECCCCCCCCCCCCEEEEECCEEEEEEECHHHHH
ALMRDGDDVVDRYALDSLRLLGSVGEPISADAWLWFHEKVGRGRCPVVDTWWQTETGGIL
HHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEHHCCCCCCCEEECCCCCCCCCEE
IAPVPGAVPQKPGAATLPLPGVACVLLTDKGAVIEGEGEGCLCVARSWPGQARTIWADHA
EECCCCCCCCCCCCCCCCCCCEEEEEEECCCCEEECCCCCEEEEEECCCCCCEEEEHHHH
RFRQTYFTFSPGYYFSGDGARRDADGYYWITGRIDDVINVSGHRIGTAEIEDVVATDHRF
HHEEEEEEECCCEEECCCCCCCCCCCEEEEEECCCCEEECCCCEECCHHHHHHHHCCCCE
VECAAVGIPHDLKGQGLVVFAVARDMAEPGLAEAAALAVTRGVGRYAVPERVYLVPDLPK
EEEEEECCCCCCCCCCEEEEEEEHHCCCCCHHHHHHHHHHHCCCCCCCCCEEEEECCCCC
TRSGKIVRRLLRKIACHELDGLGDLSTLADPTIVDRLIAEVARVA
CCCCHHHHHHHHHHHHHHCCCCCCHHHHCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA