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The map label for this gene is dadB [H]

Identifier: 162148204

GI number: 162148204

Start: 2507879

End: 2509030

Strand: Direct

Name: dadB [H]

Synonym: GDI_2421

Alternate gene names: 162148204

Gene position: 2507879-2509030 (Clockwise)

Preceding gene: 162148200

Following gene: 162148205

Centisome position: 63.58

GC content: 69.01

Gene sequence:

>1152_bases
ATGCCTGTTCCCCCCATTGCCGATACCGCATGGCCCCATGCCCGGGCCGGCGCGACCCTGACCATCGATCTTGATGCCAT
CCGGGCCAATTACCGCACGGTACGGAACCTGGCCGCCGGCGCGGAATGCGCTGCGGTGGTCAAGGCCGACGCCTATGGGC
TGGGGGCGGCCCGGGTCGCTCCGGCGCTGCAGGAGGAAGGAGCGCGGACATTCTTCGTCGCGCATCTGGACGAGGGCATG
GCGCTGCGACGCCATGTCAGCGCCGATGCGCGCATCATCGTCCTGCATGGCGCCGTGCCGGGGGCCGAGAAGGACTGTCT
GCATGATGACCTGATCCCGGTCCTGAACAGTGTCGACCAGATCCAGCGCTGGCGCGGGTTGGCCCATGAGGCGGGCCGCG
CATTGCCGTCGATCCTGCAACTGGATACCGGGATGTCGCGCTTCGGGCTGTCCGAGAACGAGATCCGCGCGATCGTGGCG
GACCCGGACGGCCTGCGCGGGATCGACCTGCGCCTGGTCATCAGCCACCTGGCCTGCGCGGACGACCCGGACGATCCAGC
CAGTACCCGTCAGCGTGCGCGCCTGGTACGAATGCGCGCCCTGCTGCCCCCCGCGCCGGTCAGCCTGGCCGCCTCGTCGG
GTGTCTTCCTGGGGCGGGACTTTCATTTCGATCTGGTCCGTCCCGGTGCGGCGCTGTACGGCGTGGCCCCCAACGCCGCT
ACCCCGAACCCGATGCGTCCGGTCGTACGCCTGCAGGCACGAATCCTGCAAACGCGCCATGTCAGCCCGAATGACGGGGT
GGGTTACGGCCTGACCTACCGCCCCAGTGCGATGCGGCGAATCGCGACGATCGCCACGGGCTATGCCGACGGTTTCATGC
GCAGCGGTGCCGCCCGGGGCTGCGCCTGGCTGGGCGACATGCAACTGCCGGTGCTGGGCCGGATTTCGATGGATTCGATG
ACGGTCGACGTCACCGATGCCCCCGAAACGCTGACCCGGCCCGGCACCATGGTAGACCTGCTGGGGCCGCGCCGTGGCGT
CGACGACGTGGCCCGGGCGGCCGGCACCATCGGCTACGAAATCCTGACATCGCTGGGGCATCGCTATCACCGCGCCTATG
TCGCGGGCGGCTCCGACGCTTTTTCCTCCTGA

Upstream 100 bases:

>100_bases
GACATATCATGCGATCCTTCGGCAGGAAATGCCGGCCATCCTGACCTAGAGTCGGGCCAGACGCCATCCCTCCCTCTCCC
TGGCGGAGCCCGCACTATTC

Downstream 100 bases:

>100_bases
CAGATCGGCACACAGGACCATGAAGATCATCATTCTCGGCAGCGGCGTCGTGGGCGTGAGTTCCGCCTGGTACCTGGCGC
AGGTTGGCCATTCGGTCACG

Product: alanine racemase, catabolic

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 383; Mature: 382

Protein sequence:

>383_residues
MPVPPIADTAWPHARAGATLTIDLDAIRANYRTVRNLAAGAECAAVVKADAYGLGAARVAPALQEEGARTFFVAHLDEGM
ALRRHVSADARIIVLHGAVPGAEKDCLHDDLIPVLNSVDQIQRWRGLAHEAGRALPSILQLDTGMSRFGLSENEIRAIVA
DPDGLRGIDLRLVISHLACADDPDDPASTRQRARLVRMRALLPPAPVSLAASSGVFLGRDFHFDLVRPGAALYGVAPNAA
TPNPMRPVVRLQARILQTRHVSPNDGVGYGLTYRPSAMRRIATIATGYADGFMRSGAARGCAWLGDMQLPVLGRISMDSM
TVDVTDAPETLTRPGTMVDLLGPRRGVDDVARAAGTIGYEILTSLGHRYHRAYVAGGSDAFSS

Sequences:

>Translated_383_residues
MPVPPIADTAWPHARAGATLTIDLDAIRANYRTVRNLAAGAECAAVVKADAYGLGAARVAPALQEEGARTFFVAHLDEGM
ALRRHVSADARIIVLHGAVPGAEKDCLHDDLIPVLNSVDQIQRWRGLAHEAGRALPSILQLDTGMSRFGLSENEIRAIVA
DPDGLRGIDLRLVISHLACADDPDDPASTRQRARLVRMRALLPPAPVSLAASSGVFLGRDFHFDLVRPGAALYGVAPNAA
TPNPMRPVVRLQARILQTRHVSPNDGVGYGLTYRPSAMRRIATIATGYADGFMRSGAARGCAWLGDMQLPVLGRISMDSM
TVDVTDAPETLTRPGTMVDLLGPRRGVDDVARAAGTIGYEILTSLGHRYHRAYVAGGSDAFSS
>Mature_382_residues
PVPPIADTAWPHARAGATLTIDLDAIRANYRTVRNLAAGAECAAVVKADAYGLGAARVAPALQEEGARTFFVAHLDEGMA
LRRHVSADARIIVLHGAVPGAEKDCLHDDLIPVLNSVDQIQRWRGLAHEAGRALPSILQLDTGMSRFGLSENEIRAIVAD
PDGLRGIDLRLVISHLACADDPDDPASTRQRARLVRMRALLPPAPVSLAASSGVFLGRDFHFDLVRPGAALYGVAPNAAT
PNPMRPVVRLQARILQTRHVSPNDGVGYGLTYRPSAMRRIATIATGYADGFMRSGAARGCAWLGDMQLPVLGRISMDSMT
VDVTDAPETLTRPGTMVDLLGPRRGVDDVARAAGTIGYEILTSLGHRYHRAYVAGGSDAFSS

Specific function: Isomerizes L-alanine to D-alanine which is then oxidized to pyruvate by DadA [H]

COG id: COG0787

COG function: function code M; Alanine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alanine racemase family [H]

Homologues:

Organism=Escherichia coli, GI1787439, Length=355, Percent_Identity=29.8591549295775, Blast_Score=135, Evalue=6e-33,
Organism=Escherichia coli, GI1790487, Length=369, Percent_Identity=29.5392953929539, Blast_Score=129, Evalue=3e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000821
- InterPro:   IPR009006
- InterPro:   IPR011079
- InterPro:   IPR001608
- InterPro:   IPR020622 [H]

Pfam domain/function: PF00842 Ala_racemase_C; PF01168 Ala_racemase_N [H]

EC number: =5.1.1.1 [H]

Molecular weight: Translated: 40837; Mature: 40706

Theoretical pI: Translated: 8.03; Mature: 8.03

Prosite motif: PS00395 ALANINE_RACEMASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPVPPIADTAWPHARAGATLTIDLDAIRANYRTVRNLAAGAECAAVVKADAYGLGAARVA
CCCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHCCCHHHEEEECCCCCCCHHHHH
PALQEEGARTFFVAHLDEGMALRRHVSADARIIVLHGAVPGAEKDCLHDDLIPVLNSVDQ
HHHHHCCCCEEEEEECCCCHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHH
IQRWRGLAHEAGRALPSILQLDTGMSRFGLSENEIRAIVADPDGLRGIDLRLVISHLACA
HHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCEEEEEECCCCCCCCHHHHHHHHHHCC
DDPDDPASTRQRARLVRMRALLPPAPVSLAASSGVFLGRDFHFDLVRPGAALYGVAPNAA
CCCCCHHHHHHHHHHHHHHHHCCCCCCHHCCCCCEEEECCCEEEEECCCCEEEECCCCCC
TPNPMRPVVRLQARILQTRHVSPNDGVGYGLTYRPSAMRRIATIATGYADGFMRSGAARG
CCCCCHHHHHHHHHHHHHCCCCCCCCCCCCEEECHHHHHHHHHHHHHHHHHHHHCCCCCC
CAWLGDMQLPVLGRISMDSMTVDVTDAPETLTRPGTMVDLLGPRRGVDDVARAAGTIGYE
CEEECCCCCCEEEEEECCCEEEEECCCCHHHCCCCCEEEECCCCCCHHHHHHHHHHHHHH
ILTSLGHRYHRAYVAGGSDAFSS
HHHHHHHHHHEEEEECCCCCCCC
>Mature Secondary Structure 
PVPPIADTAWPHARAGATLTIDLDAIRANYRTVRNLAAGAECAAVVKADAYGLGAARVA
CCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHCCCHHHEEEECCCCCCCHHHHH
PALQEEGARTFFVAHLDEGMALRRHVSADARIIVLHGAVPGAEKDCLHDDLIPVLNSVDQ
HHHHHCCCCEEEEEECCCCHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHH
IQRWRGLAHEAGRALPSILQLDTGMSRFGLSENEIRAIVADPDGLRGIDLRLVISHLACA
HHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCEEEEEECCCCCCCCHHHHHHHHHHCC
DDPDDPASTRQRARLVRMRALLPPAPVSLAASSGVFLGRDFHFDLVRPGAALYGVAPNAA
CCCCCHHHHHHHHHHHHHHHHCCCCCCHHCCCCCEEEECCCEEEEECCCCEEEECCCCCC
TPNPMRPVVRLQARILQTRHVSPNDGVGYGLTYRPSAMRRIATIATGYADGFMRSGAARG
CCCCCHHHHHHHHHHHHHCCCCCCCCCCCCEEECHHHHHHHHHHHHHHHHHHHHCCCCCC
CAWLGDMQLPVLGRISMDSMTVDVTDAPETLTRPGTMVDLLGPRRGVDDVARAAGTIGYE
CEEECCCCCCEEEEEECCCEEEEECCCCHHHCCCCCEEEECCCCCCHHHHHHHHHHHHHH
ILTSLGHRYHRAYVAGGSDAFSS
HHHHHHHHHHEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11214968 [H]