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The map label for this gene is leuS [H]

Identifier: 162148176

GI number: 162148176

Start: 2469763

End: 2472393

Strand: Direct

Name: leuS [H]

Synonym: GDI_2393

Alternate gene names: 162148176

Gene position: 2469763-2472393 (Clockwise)

Preceding gene: 162148175

Following gene: 162148177

Centisome position: 62.62

GC content: 69.29

Gene sequence:

>2631_bases
GTGCCCCGGACCATGATCATGACCGAGAATTCCCCCGCCCCCTATGATTTCCGCACGGCCGAGCCGCACTGGCAGGCCGA
ATGGGACCGGGCGGGGGCTTTCGACGTGCCCGACGTGCCCCCCGCCGACCGCCCGAAATATTACGTGCTGGAGATGTTCC
CCTACCCGTCGGGGCAGCTTCATATGGGCCATGTCCGCAACTATACGCTGGGCGACGTGGTGGCTCGGTACAAGCGGGCC
CGCGGCCATTCGGTGCTGCACCCGATGGGCTGGACGCGTTCGGCCTGCCGGCGGAAAACGCGGCGCGCGAACGCGGCGTG
CACCCGCAGGCCTGGACCTGGACAAATCGCCGCCATGCGCGCGACGCTGAAGCGCCTGGGCTTCTCGTTCAACTGGGACC
GCGAGATCGCGACCTGCCTGCCGGACTATTACGGCAAGCAGCAGAAGCTGTTCCTGGACATGCTGGGGGCCGGCCTGGTC
GAACGCCGCGAAAGCTGGGTGAACTGGGACCCGGTGGACCATACGGTGCTGGCGAACGAACAGGTGGTGGACGGCCGCGG
CTGGCGGTCCGGCGCGCTGATCGAAAAGAAGCAGCTTTCGCAGTGGTTCCTGCGCATCACCAAATTCGCGCCCGACCTGC
TGGACGGGCTGTCGCACCTGCCCCGCTGGCCCGAGCGGGTACGGACGATGCAGGAACGCTGGATCGGCCGGTCGGTCGGC
GCGCGGGTGCGTTTTGCCCTGCATGCCCCGCCCGAGGGTTTCGACACCGACCTCGATTCGGTCGAGGTCTTCACCACGCG
GCCGGATACGCTGTTCGGCATGGCCTTCCTCGCCGTCGCGGCGGACCACCCGCTGGCGGCCAAGGTGGCGCAGGACAATC
CCGCCGCCGCCGCGTTCATCGCCGAATGCCGCCGCCTGGGCACGTCCGAGGAAGCGATCGAAACGGCGGAAAAGCGCGGT
TTCGATACCGGGTTGCGGGTATCGCATCCCTTCCTGCCGGATCGCAGCTTCCCGGTCTGGATCGCCAATTTCGTGCTGAT
GGATTACGGCACGGGCGCGATCTTCGGCTGCCCCTGCGGTGACCAGCGCGACCTGGATTTCGCCAATGCCTATCACCTGC
CGTTCCAGCCGGTGATCCTGCCGCCCGGCGAGGACGCCACGACCTTCACCATTACCGGCAAGGCGTGGGACGGTGACGGC
ACGCTGTTCAATTCCGGCTTCCTCGACGGGCTGGATTCGGCCACCGCGAAGGCCGAGGCGATCACCCGGCTGGAAGGCAT
GGGCGTCGGCCGGGGCGTGGTGAACTGGCGCCTGCGCGACTGGGGCATCTCGCGCCAGCGGTACTGGGGCTGCCCGATCC
CGGTGATCCATTGCGCGTCCTGCGGCGCGGTGCCGGTGCCCGACGACCGGTTGCCCGTGGTGCTGCCCGAGGACGTGACG
TTCGACCGTCCCGGCAATCCGCTGGACCATCATCCGACCTGGAAGCACGTCGCCTGCCCGCGTTGCGGCGCCGCCGCCAC
GCGTGAAACCGATACGTTCGACACGTTCGTGGACAGTTCGTGGTACTTCGCCCGCTTCACCGCGCCGCACGCGGCAGCGC
CGACCGTGCGCCCGGCGGCGGACGGCTGGCTGCCGGTCGACCAGTATATCGGCGGCATCGAACACGCGATCCTGCATCTG
CTCTATGCCCGCTTCTTCACCCGGGCGATGCGCGAAACCGGGCATGTCGGGCTGGACGAGCCGTTCGACGGCCTGTTCAC
CCAGGGCATGGTGAACCACGAGAGCTATCGCGACGCGGCGGGCCAGTGGCTGTATCCCGAGGAAGTCGACCGCCGTGCCG
ATGGCGCGGTCAGCCGCGATACCGGCGCCCCGGTGACGATCGGCCGCGTCGAGAAAATGTCCAAGTCCAAGCGCAACACC
GTAGCGCCGGTCGCGATCATCGAACGGTTCGGCGCCGATACCGCACGCTGGTTCGTGCTGTCGGACAGCCCGCCGGAACG
CGACATGGAATGGACCGAGGCCGGCGTGGCCGGCGCCGCGCGCTTCCTGCAGCGGCTGTACCGGACCGTGCGCACCGTCG
CCGAATCCGTGCAGCCCGTGGCGGAAGCCGCCGCCACGCCCACGGAAGCCGCCGATACGCTGCGCCGGGCCACCCACCGC
GCCATCGCCGCCGTGACCGACGCGCTGGAAAGCTTCGCGATCAACGTCGCGGTGGCCCGCCTGCACGAACTGACGACCGC
GCTGGCGGAGTCCGAACGCGCGTCAGGCGACGACGGCATGGATTTCGCCCGGCGCGAGGCCGCGCTGACCCTGTGCCTGC
TGGCCGCCCCGATGGTGCCGCACCAGGCCGAGGACATGCTGGCCCTGCTGGAGCCCGGACAATCCCCCGCCGTCAGCCGC
CCGTGGCCGGTCGCCGAGCCCGAACTGCTGGTCGCCCGCCAGGTCACGATCGCGGTTCAGATCATGGGCAAGCTGCGCGG
CACGGTCGCGGCCCCGCCCGACGCCCCGGCGGATGCGGTCATCGCCCTGGCGGAAACGGAGACCAACGTCGCCCGGCTGC
TGGAGGGCAAGCGCATCGTCAAGCGCATCCACGTGCCCAACCGCATCGTCAATTTCGTGATCGCGGGCTGA

Upstream 100 bases:

>100_bases
CAGCTTCGCGCCGACAGCGGCGGCCACTGACCCGCGCCGCCCCGGCGCGGCCCGCTTCCCTGCATCGATCGATCAGGGCC
CCCATAGACGATCGGGCCAC

Downstream 100 bases:

>100_bases
CCCGATGACCGCCCCGCCGCGCCGCGCCGTCCTTGGGGCCCTGTTGCTGGCCGGGTCGCTGTCCCTGGGCGGATGCGGCT
TCCAGCCCCTCTATGGCGGC

Product: leucyl-tRNA synthetase

Products: NA

Alternate protein names: Leucine--tRNA ligase; LeuRS [H]

Number of amino acids: Translated: 876; Mature: 875

Protein sequence:

>876_residues
MPRTMIMTENSPAPYDFRTAEPHWQAEWDRAGAFDVPDVPPADRPKYYVLEMFPYPSGQLHMGHVRNYTLGDVVARYKRA
RGHSVLHPMGWTRSACRRKTRRANAACTRRPGPGQIAAMRATLKRLGFSFNWDREIATCLPDYYGKQQKLFLDMLGAGLV
ERRESWVNWDPVDHTVLANEQVVDGRGWRSGALIEKKQLSQWFLRITKFAPDLLDGLSHLPRWPERVRTMQERWIGRSVG
ARVRFALHAPPEGFDTDLDSVEVFTTRPDTLFGMAFLAVAADHPLAAKVAQDNPAAAAFIAECRRLGTSEEAIETAEKRG
FDTGLRVSHPFLPDRSFPVWIANFVLMDYGTGAIFGCPCGDQRDLDFANAYHLPFQPVILPPGEDATTFTITGKAWDGDG
TLFNSGFLDGLDSATAKAEAITRLEGMGVGRGVVNWRLRDWGISRQRYWGCPIPVIHCASCGAVPVPDDRLPVVLPEDVT
FDRPGNPLDHHPTWKHVACPRCGAAATRETDTFDTFVDSSWYFARFTAPHAAAPTVRPAADGWLPVDQYIGGIEHAILHL
LYARFFTRAMRETGHVGLDEPFDGLFTQGMVNHESYRDAAGQWLYPEEVDRRADGAVSRDTGAPVTIGRVEKMSKSKRNT
VAPVAIIERFGADTARWFVLSDSPPERDMEWTEAGVAGAARFLQRLYRTVRTVAESVQPVAEAAATPTEAADTLRRATHR
AIAAVTDALESFAINVAVARLHELTTALAESERASGDDGMDFARREAALTLCLLAAPMVPHQAEDMLALLEPGQSPAVSR
PWPVAEPELLVARQVTIAVQIMGKLRGTVAAPPDAPADAVIALAETETNVARLLEGKRIVKRIHVPNRIVNFVIAG

Sequences:

>Translated_876_residues
MPRTMIMTENSPAPYDFRTAEPHWQAEWDRAGAFDVPDVPPADRPKYYVLEMFPYPSGQLHMGHVRNYTLGDVVARYKRA
RGHSVLHPMGWTRSACRRKTRRANAACTRRPGPGQIAAMRATLKRLGFSFNWDREIATCLPDYYGKQQKLFLDMLGAGLV
ERRESWVNWDPVDHTVLANEQVVDGRGWRSGALIEKKQLSQWFLRITKFAPDLLDGLSHLPRWPERVRTMQERWIGRSVG
ARVRFALHAPPEGFDTDLDSVEVFTTRPDTLFGMAFLAVAADHPLAAKVAQDNPAAAAFIAECRRLGTSEEAIETAEKRG
FDTGLRVSHPFLPDRSFPVWIANFVLMDYGTGAIFGCPCGDQRDLDFANAYHLPFQPVILPPGEDATTFTITGKAWDGDG
TLFNSGFLDGLDSATAKAEAITRLEGMGVGRGVVNWRLRDWGISRQRYWGCPIPVIHCASCGAVPVPDDRLPVVLPEDVT
FDRPGNPLDHHPTWKHVACPRCGAAATRETDTFDTFVDSSWYFARFTAPHAAAPTVRPAADGWLPVDQYIGGIEHAILHL
LYARFFTRAMRETGHVGLDEPFDGLFTQGMVNHESYRDAAGQWLYPEEVDRRADGAVSRDTGAPVTIGRVEKMSKSKRNT
VAPVAIIERFGADTARWFVLSDSPPERDMEWTEAGVAGAARFLQRLYRTVRTVAESVQPVAEAAATPTEAADTLRRATHR
AIAAVTDALESFAINVAVARLHELTTALAESERASGDDGMDFARREAALTLCLLAAPMVPHQAEDMLALLEPGQSPAVSR
PWPVAEPELLVARQVTIAVQIMGKLRGTVAAPPDAPADAVIALAETETNVARLLEGKRIVKRIHVPNRIVNFVIAG
>Mature_875_residues
PRTMIMTENSPAPYDFRTAEPHWQAEWDRAGAFDVPDVPPADRPKYYVLEMFPYPSGQLHMGHVRNYTLGDVVARYKRAR
GHSVLHPMGWTRSACRRKTRRANAACTRRPGPGQIAAMRATLKRLGFSFNWDREIATCLPDYYGKQQKLFLDMLGAGLVE
RRESWVNWDPVDHTVLANEQVVDGRGWRSGALIEKKQLSQWFLRITKFAPDLLDGLSHLPRWPERVRTMQERWIGRSVGA
RVRFALHAPPEGFDTDLDSVEVFTTRPDTLFGMAFLAVAADHPLAAKVAQDNPAAAAFIAECRRLGTSEEAIETAEKRGF
DTGLRVSHPFLPDRSFPVWIANFVLMDYGTGAIFGCPCGDQRDLDFANAYHLPFQPVILPPGEDATTFTITGKAWDGDGT
LFNSGFLDGLDSATAKAEAITRLEGMGVGRGVVNWRLRDWGISRQRYWGCPIPVIHCASCGAVPVPDDRLPVVLPEDVTF
DRPGNPLDHHPTWKHVACPRCGAAATRETDTFDTFVDSSWYFARFTAPHAAAPTVRPAADGWLPVDQYIGGIEHAILHLL
YARFFTRAMRETGHVGLDEPFDGLFTQGMVNHESYRDAAGQWLYPEEVDRRADGAVSRDTGAPVTIGRVEKMSKSKRNTV
APVAIIERFGADTARWFVLSDSPPERDMEWTEAGVAGAARFLQRLYRTVRTVAESVQPVAEAAATPTEAADTLRRATHRA
IAAVTDALESFAINVAVARLHELTTALAESERASGDDGMDFARREAALTLCLLAAPMVPHQAEDMLALLEPGQSPAVSRP
WPVAEPELLVARQVTIAVQIMGKLRGTVAAPPDAPADAVIALAETETNVARLLEGKRIVKRIHVPNRIVNFVIAG

Specific function: Unknown

COG id: COG0495

COG function: function code J; Leucyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI7661872, Length=867, Percent_Identity=36.1014994232987, Blast_Score=489, Evalue=1e-138,
Organism=Escherichia coli, GI1786861, Length=870, Percent_Identity=43.2183908045977, Blast_Score=686, Evalue=0.0,
Organism=Caenorhabditis elegans, GI71997510, Length=288, Percent_Identity=36.1111111111111, Blast_Score=179, Evalue=5e-45,
Organism=Caenorhabditis elegans, GI71997517, Length=285, Percent_Identity=36.4912280701754, Blast_Score=179, Evalue=6e-45,
Organism=Caenorhabditis elegans, GI212645227, Length=319, Percent_Identity=26.3322884012539, Blast_Score=129, Evalue=1e-29,
Organism=Saccharomyces cerevisiae, GI6323414, Length=888, Percent_Identity=35.2477477477478, Blast_Score=516, Evalue=1e-147,
Organism=Drosophila melanogaster, GI21355409, Length=787, Percent_Identity=34.9428208386277, Blast_Score=396, Evalue=1e-110,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR002300
- InterPro:   IPR002302
- InterPro:   IPR014729
- InterPro:   IPR009080
- InterPro:   IPR013155
- InterPro:   IPR009008 [H]

Pfam domain/function: PF08264 Anticodon_1; PF00133 tRNA-synt_1 [H]

EC number: =6.1.1.4 [H]

Molecular weight: Translated: 96868; Mature: 96737

Theoretical pI: Translated: 6.72; Mature: 6.72

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRTMIMTENSPAPYDFRTAEPHWQAEWDRAGAFDVPDVPPADRPKYYVLEMFPYPSGQL
CCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCE
HMGHVRNYTLGDVVARYKRARGHSVLHPMGWTRSACRRKTRRANAACTRRPGPGQIAAMR
EECCCCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHH
ATLKRLGFSFNWDREIATCLPDYYGKQQKLFLDMLGAGLVERRESWVNWDPVDHTVLANE
HHHHHHCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCC
QVVDGRGWRSGALIEKKQLSQWFLRITKFAPDLLDGLSHLPRWPERVRTMQERWIGRSVG
EEECCCCCCCCCCHHHHHHHHHHHHHHHHCHHHHHHHHHCCCCHHHHHHHHHHHHCCCCC
ARVRFALHAPPEGFDTDLDSVEVFTTRPDTLFGMAFLAVAADHPLAAKVAQDNPAAAAFI
CEEEEEEECCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHCCCCCEEEECCCCCHHHHHH
AECRRLGTSEEAIETAEKRGFDTGLRVSHPFLPDRSFPVWIANFVLMDYGTGAIFGCPCG
HHHHHCCCCHHHHHHHHHCCCCCCCEECCCCCCCCCCCHHHHHHHHHCCCCCCEEECCCC
DQRDLDFANAYHLPFQPVILPPGEDATTFTITGKAWDGDGTLFNSGFLDGLDSATAKAEA
CCCCCCHHCEECCCCCCEEECCCCCCEEEEEEEEEECCCCCEECCCCCCCCCHHHHHHHH
ITRLEGMGVGRGVVNWRLRDWGISRQRYWGCPIPVIHCASCGAVPVPDDRLPVVLPEDVT
HHHHHCCCCCCCEEEEEECCCCCCCCCCCCCCCHHHHHCCCCCCCCCCCCCCEECCCCCC
FDRPGNPLDHHPTWKHVACPRCGAAATRETDTFDTFVDSSWYFARFTAPHAAAPTVRPAA
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCEEEEEECCCCCCCCCCCCCC
DGWLPVDQYIGGIEHAILHLLYARFFTRAMRETGHVGLDEPFDGLFTQGMVNHESYRDAA
CCCEEHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCCHHHHHHC
GQWLYPEEVDRRADGAVSRDTGAPVTIGRVEKMSKSKRNTVAPVAIIERFGADTARWFVL
CCCCCHHHHHHHHCCCCCCCCCCCEEHHHHHHHHHHHCCCCHHHHHHHHHCCCCEEEEEE
SDSPPERDMEWTEAGVAGAARFLQRLYRTVRTVAESVQPVAEAAATPTEAADTLRRATHR
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
AIAAVTDALESFAINVAVARLHELTTALAESERASGDDGMDFARREAALTLCLLAAPMVP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCC
HQAEDMLALLEPGQSPAVSRPWPVAEPELLVARQVTIAVQIMGKLRGTVAAPPDAPADAV
CCHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCE
IALAETETNVARLLEGKRIVKRIHVPNRIVNFVIAG
EEEECCHHHHHHHHHHHHHHHHHCCCHHHHHEEECC
>Mature Secondary Structure 
PRTMIMTENSPAPYDFRTAEPHWQAEWDRAGAFDVPDVPPADRPKYYVLEMFPYPSGQL
CCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCE
HMGHVRNYTLGDVVARYKRARGHSVLHPMGWTRSACRRKTRRANAACTRRPGPGQIAAMR
EECCCCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHH
ATLKRLGFSFNWDREIATCLPDYYGKQQKLFLDMLGAGLVERRESWVNWDPVDHTVLANE
HHHHHHCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCC
QVVDGRGWRSGALIEKKQLSQWFLRITKFAPDLLDGLSHLPRWPERVRTMQERWIGRSVG
EEECCCCCCCCCCHHHHHHHHHHHHHHHHCHHHHHHHHHCCCCHHHHHHHHHHHHCCCCC
ARVRFALHAPPEGFDTDLDSVEVFTTRPDTLFGMAFLAVAADHPLAAKVAQDNPAAAAFI
CEEEEEEECCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHCCCCCEEEECCCCCHHHHHH
AECRRLGTSEEAIETAEKRGFDTGLRVSHPFLPDRSFPVWIANFVLMDYGTGAIFGCPCG
HHHHHCCCCHHHHHHHHHCCCCCCCEECCCCCCCCCCCHHHHHHHHHCCCCCCEEECCCC
DQRDLDFANAYHLPFQPVILPPGEDATTFTITGKAWDGDGTLFNSGFLDGLDSATAKAEA
CCCCCCHHCEECCCCCCEEECCCCCCEEEEEEEEEECCCCCEECCCCCCCCCHHHHHHHH
ITRLEGMGVGRGVVNWRLRDWGISRQRYWGCPIPVIHCASCGAVPVPDDRLPVVLPEDVT
HHHHHCCCCCCCEEEEEECCCCCCCCCCCCCCCHHHHHCCCCCCCCCCCCCCEECCCCCC
FDRPGNPLDHHPTWKHVACPRCGAAATRETDTFDTFVDSSWYFARFTAPHAAAPTVRPAA
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCEEEEEECCCCCCCCCCCCCC
DGWLPVDQYIGGIEHAILHLLYARFFTRAMRETGHVGLDEPFDGLFTQGMVNHESYRDAA
CCCEEHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCCHHHHHHC
GQWLYPEEVDRRADGAVSRDTGAPVTIGRVEKMSKSKRNTVAPVAIIERFGADTARWFVL
CCCCCHHHHHHHHCCCCCCCCCCCEEHHHHHHHHHHHCCCCHHHHHHHHHCCCCEEEEEE
SDSPPERDMEWTEAGVAGAARFLQRLYRTVRTVAESVQPVAEAAATPTEAADTLRRATHR
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
AIAAVTDALESFAINVAVARLHELTTALAESERASGDDGMDFARREAALTLCLLAAPMVP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCC
HQAEDMLALLEPGQSPAVSRPWPVAEPELLVARQVTIAVQIMGKLRGTVAAPPDAPADAV
CCHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCE
IALAETETNVARLLEGKRIVKRIHVPNRIVNFVIAG
EEEECCHHHHHHHHHHHHHHHHHCCCHHHHHEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA