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The map label for this gene is rih [H]

Identifier: 162148170

GI number: 162148170

Start: 2462671

End: 2463861

Strand: Direct

Name: rih [H]

Synonym: GDI_2387

Alternate gene names: 162148170

Gene position: 2462671-2463861 (Clockwise)

Preceding gene: 162148169

Following gene: 162148171

Centisome position: 62.44

GC content: 66.92

Gene sequence:

>1191_bases
ATGTTTTTCTGCCCTCTCCGGCGCGCGATGCGCCTCGGGACGACCCGACGCGAATCGACGCCGCGCGAAGTGCCGCGCCG
CGCCTTCCGCCGCGCCCTGGGCGCCGGCGTGCTCGGCCTGCTGGCCGCCTGGCTGACGGCCGGGCCGCCCGCGCGGGCGG
CCGCCCCGCAACTGGTGATCGAGGACAATGATTTCCTCGGTCCCGGCGGATCGGACCAGCAATCGATCATTCCGCTGCTG
TTCAATCCGGCGGTCCGGGTCCTGGGCTTCACCGTCGTGACCGGCGATGGCTGGGAAAACCAGGAATCCGCCCATCTGCG
CCGGTTCCTGGACATTATCGGCCGGGGCGACGTGCCGGTGGCGGACGGCGCGGTCTATCCGCTGATCAACAGCGTGCCGA
TGATGCGCCTGCGCGAACAGCAATACGGCGTCATCCCGTGGAAGGGCGCCTGGGGCGGAATCGGGTCGATCGACGGCACG
CCCGCGACCCAGCCCCCCTTGCCGGACCTGAAGGAGGGCGCCCCGGCACAGCGTCCGATCGATGACAGTGCGGCCCTGTT
CCTGATCCGTCAGGTCCACAATCACCCCCATCAGGTCACCATCGTGGCCGCCGGGCCGCTGACCAACCTGGCGCTGGCGA
TCCGCATCGACCCGACCTTCGCCGCGACGGCGAAACAACTGGTGTTCATGGGCGGATTGCTGGACACCAGCATGAAATCG
CTGACCGGGAACGTCGATTTCGCCTCGGACTTCAACATGATCTTCGACCCGGAGGCGGCGCATATCACGCTGACGGCACC
GTGGCCGTCGATCACCGTCGTGGGCAACGTGTCGAACGACCTGATGATGACGCGCGACTACCTGAAGCGCATCACGCAGA
AGAAGACGAAAATGACGGACTATATCGGCACCTATTACGACCCGCTGCCGATGTGGGACGAACTGGCGACGGCGATCGCC
GCCGACCCCGGCCTGATCACGTCCTCGATCGACGCCTATATGGACATCGACATTTCCAAGGGGCCGCAATACGGCCACGC
CGTCGTCTGGCCGGATGCCACCGCGCCCAGGGCGATGGGCGTCCGCAAGGTGCGGATCGTGCAGTCCGTGGATGCGGGAC
GCTTCCTCGCCACGTTCCTGCACGAGGCCCAGAGCGACATCCATCCGTCCCATGCCTTCCCCCGGCCCTGA

Upstream 100 bases:

>100_bases
ACCGGTCCTGCGCGCAACACCGTCGGGCCGCCTCCGCCTGGAAGCGCTGCTCGGCGCCCTGGTGCTGTAGGACCCGGCCC
ACCAGCATTTAGGACGCCGC

Downstream 100 bases:

>100_bases
CGATCCCATGTTGCACATGGTCAAGCTGGCGGTCGGAATCGCATCGATCGACGACCTGGCGCATCGTCAGCGCACGATGA
TGGAGGGCGCCGGAGATGGG

Product: pyrimidine-specific ribonucleoside hydrolase

Products: uracil; ribose; cytosine [C]

Alternate protein names: Cytidine/uridine-specific hydrolase [H]

Number of amino acids: Translated: 396; Mature: 396

Protein sequence:

>396_residues
MFFCPLRRAMRLGTTRRESTPREVPRRAFRRALGAGVLGLLAAWLTAGPPARAAAPQLVIEDNDFLGPGGSDQQSIIPLL
FNPAVRVLGFTVVTGDGWENQESAHLRRFLDIIGRGDVPVADGAVYPLINSVPMMRLREQQYGVIPWKGAWGGIGSIDGT
PATQPPLPDLKEGAPAQRPIDDSAALFLIRQVHNHPHQVTIVAAGPLTNLALAIRIDPTFAATAKQLVFMGGLLDTSMKS
LTGNVDFASDFNMIFDPEAAHITLTAPWPSITVVGNVSNDLMMTRDYLKRITQKKTKMTDYIGTYYDPLPMWDELATAIA
ADPGLITSSIDAYMDIDISKGPQYGHAVVWPDATAPRAMGVRKVRIVQSVDAGRFLATFLHEAQSDIHPSHAFPRP

Sequences:

>Translated_396_residues
MFFCPLRRAMRLGTTRRESTPREVPRRAFRRALGAGVLGLLAAWLTAGPPARAAAPQLVIEDNDFLGPGGSDQQSIIPLL
FNPAVRVLGFTVVTGDGWENQESAHLRRFLDIIGRGDVPVADGAVYPLINSVPMMRLREQQYGVIPWKGAWGGIGSIDGT
PATQPPLPDLKEGAPAQRPIDDSAALFLIRQVHNHPHQVTIVAAGPLTNLALAIRIDPTFAATAKQLVFMGGLLDTSMKS
LTGNVDFASDFNMIFDPEAAHITLTAPWPSITVVGNVSNDLMMTRDYLKRITQKKTKMTDYIGTYYDPLPMWDELATAIA
ADPGLITSSIDAYMDIDISKGPQYGHAVVWPDATAPRAMGVRKVRIVQSVDAGRFLATFLHEAQSDIHPSHAFPRP
>Mature_396_residues
MFFCPLRRAMRLGTTRRESTPREVPRRAFRRALGAGVLGLLAAWLTAGPPARAAAPQLVIEDNDFLGPGGSDQQSIIPLL
FNPAVRVLGFTVVTGDGWENQESAHLRRFLDIIGRGDVPVADGAVYPLINSVPMMRLREQQYGVIPWKGAWGGIGSIDGT
PATQPPLPDLKEGAPAQRPIDDSAALFLIRQVHNHPHQVTIVAAGPLTNLALAIRIDPTFAATAKQLVFMGGLLDTSMKS
LTGNVDFASDFNMIFDPEAAHITLTAPWPSITVVGNVSNDLMMTRDYLKRITQKKTKMTDYIGTYYDPLPMWDELATAIA
ADPGLITSSIDAYMDIDISKGPQYGHAVVWPDATAPRAMGVRKVRIVQSVDAGRFLATFLHEAQSDIHPSHAFPRP

Specific function: Hydrolyzes cytidine or uridine to ribose and cytosine or uracil, respectively [H]

COG id: COG1957

COG function: function code F; Inosine-uridine nucleoside N-ribohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IUNH family. RihA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786871, Length=204, Percent_Identity=27.9411764705882, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI1788486, Length=304, Percent_Identity=24.6710526315789, Blast_Score=71, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI32565474, Length=282, Percent_Identity=29.7872340425532, Blast_Score=92, Evalue=3e-19,
Organism=Drosophila melanogaster, GI24641837, Length=231, Percent_Identity=32.9004329004329, Blast_Score=66, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015910
- InterPro:   IPR023186
- InterPro:   IPR001910
- InterPro:   IPR022975 [H]

Pfam domain/function: PF01156 IU_nuc_hydro [H]

EC number: NA

Molecular weight: Translated: 43103; Mature: 43103

Theoretical pI: Translated: 7.35; Mature: 7.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFFCPLRRAMRLGTTRRESTPREVPRRAFRRALGAGVLGLLAAWLTAGPPARAAAPQLVI
CCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCEEEE
EDNDFLGPGGSDQQSIIPLLFNPAVRVLGFTVVTGDGWENQESAHLRRFLDIIGRGDVPV
ECCCCCCCCCCCCCCCHHHHHHHHHHHHEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCC
ADGAVYPLINSVPMMRLREQQYGVIPWKGAWGGIGSIDGTPATQPPLPDLKEGAPAQRPI
CCCCHHHHHHCCHHHHHHHHHCCCEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
DDSAALFLIRQVHNHPHQVTIVAAGPLTNLALAIRIDPTFAATAKQLVFMGGLLDTSMKS
CCHHHHHHHHHHHCCCCEEEEEEECCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHH
LTGNVDFASDFNMIFDPEAAHITLTAPWPSITVVGNVSNDLMMTRDYLKRITQKKTKMTD
HCCCCCCCCCCCEEECCCCCEEEEECCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHH
YIGTYYDPLPMWDELATAIAADPGLITSSIDAYMDIDISKGPQYGHAVVWPDATAPRAMG
HHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCCCCCEEECCCCCCCHHHC
VRKVRIVQSVDAGRFLATFLHEAQSDIHPSHAFPRP
CEEEEEEHHCCCHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MFFCPLRRAMRLGTTRRESTPREVPRRAFRRALGAGVLGLLAAWLTAGPPARAAAPQLVI
CCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCEEEE
EDNDFLGPGGSDQQSIIPLLFNPAVRVLGFTVVTGDGWENQESAHLRRFLDIIGRGDVPV
ECCCCCCCCCCCCCCCHHHHHHHHHHHHEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCC
ADGAVYPLINSVPMMRLREQQYGVIPWKGAWGGIGSIDGTPATQPPLPDLKEGAPAQRPI
CCCCHHHHHHCCHHHHHHHHHCCCEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
DDSAALFLIRQVHNHPHQVTIVAAGPLTNLALAIRIDPTFAATAKQLVFMGGLLDTSMKS
CCHHHHHHHHHHHCCCCEEEEEEECCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHH
LTGNVDFASDFNMIFDPEAAHITLTAPWPSITVVGNVSNDLMMTRDYLKRITQKKTKMTD
HCCCCCCCCCCCEEECCCCCEEEEECCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHH
YIGTYYDPLPMWDELATAIAADPGLITSSIDAYMDIDISKGPQYGHAVVWPDATAPRAMG
HHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCCCCCEEECCCCCCCHHHC
VRKVRIVQSVDAGRFLATFLHEAQSDIHPSHAFPRP
CEEEEEEHHCCCHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uridine; H2O; cytidine [C]

Specific reaction: uridine + H2O = uracil + ribose cytidine + H2O = cytosine + ribose [C]

General reaction: Pyrimidine-specific nucleoside hydrolase [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA