Click here to switch to the map view.

The map label for this gene is nrgA [H]

Identifier: 162148135

GI number: 162148135

Start: 2423527

End: 2424918

Strand: Direct

Name: nrgA [H]

Synonym: GDI_2352

Alternate gene names: 162148135

Gene position: 2423527-2424918 (Clockwise)

Preceding gene: 162148134

Following gene: 162148136

Centisome position: 61.45

GC content: 68.61

Gene sequence:

>1392_bases
GTGAGTCTCCCCCGCACTATTCGGACTTTCTCACCTCGCGGCACCACCATCGCCCTGGCCACGCTGGCCGGGGCGGCGCT
GCCGGCCATGGCCGCGCCCGCCATGGCCGCCGATCCGGCCCCGCCCGCCATCAACACCGGCGATACCCCCTGGATGCTGG
TCAGCACGCGCTGGGTGCTGATGATGACCGTACCGGGCCTGGCCCTGTTCTATGGCGGCATGGTCCGCAAGAAGAACGTG
CTGGCCACGCTGATGCAGTCCTTCGCCATCTGCTGCATCGTCACGATTGTCTGGATGGTCGCGGGCTACAGCCTGGCGTT
CGGCACCGGGTCGCCCTACATCGGCGATCTGTCCCGCTTCATGCTGAACGGCATCGGCGCGCAGATTTCCAAGGGGTCCG
ATGTCGGCTTCACGCTGGGGCTGGGGTCGGCCAACGCCACGGTGATGACGATCCCCGAGAGCGTCTTCATGATGTTCCAG
ATGACGTTCGCGATCATCACCCCGGCGCTGATCACCGGCGCCTTCGCCGAGCGCATGAAGTTCAGCGCGCTGTGCGTGTT
CACCATCCTGTGGTCGCTGCTGGTCTATGCGCCGGTCGCCCACTGGGTCTGGAGCCCGCTTGGCTGGGTCGCGGGGTTCG
GCGCCATCGATTTCGCCGGCGGCACCGTGGTGCACATCAATGCCGGCGTCGCCGGCCTGGTCACGGCGCTGGTGGTGGGC
AAGCGGCAGGGCTACGGCCAGGACGATATGTCGCCCTTCAACCTGACCTATGCCGTCATCGGCGCGTCGCTGCTGTGGGT
CGGCTGGTTCGGCTTCAATGCCGGGTCGGCGGTAGGGGCCAACGGCCGCGCCGGCATGGCGATGGCGACGACGCAGATCG
CCAGCGCCGCCGCCGGGGTGGCCTGGATGCTGGCCGAATGGGCCCGCACCGGCAAGCCGACGGTGCTGGGCATCATTTCC
GGCGCCGTCGGCGGACTGGTCGCGATCACGCCCGCCGCCGGCTTCGTGCTGCCGGGCGGGGCCCTGATCATCGGCCTGCT
GGCCGGCGCGGTCTGCTACTGGACCGCCACCACCATGAAGCACATGCTGGGCTATGACGACAGCCTGGACGCCTTCGGCG
TGCATGGCATCGGCGGCATCCTGGGCGCGCTGCTGACCGGCGTGCTGGCCTATGGCCCGCTTTCGGCCACCGACGCCAAT
CCCGCCGGGGTCGTGGGGTCCTTCGCGCAGCTCGTCACCCAGGCCAAGGCCGTGGGCGTCACCATCGTCTGGTGCGCGGT
CGTCACCTTCGTCCTGCTGAAGATCGTCGACCTCGCCATCGGCCTGCGCGTCCGGAGCGAGGACGAAATCGAGGGGCTGG
ACATGACCCAGCACGGCGAACGCATCAACTGA

Upstream 100 bases:

>100_bases
AGTCCGCCCACACGGGCAAGATCGGCGACGGCAAGATCTTCGTATCCAGCCTGGACAGCGTCATCCGCATCCGGACGCGC
GAGACGGGAGAAGACGCGCT

Downstream 100 bases:

>100_bases
TCCATTTCACCGAACAACAGACAGGCCGGCCCGGGTGCATGCCCGGGCCGGCCTTTTTCTTTCCGTATTCAAGTCAAACT
GTTATGGATGATTGTCCAGA

Product: ammonium transporter

Products: NH3 [Cytoplasm] [C]

Alternate protein names: Membrane protein NrgA; Protein AmtB [H]

Number of amino acids: Translated: 463; Mature: 462

Protein sequence:

>463_residues
MSLPRTIRTFSPRGTTIALATLAGAALPAMAAPAMAADPAPPAINTGDTPWMLVSTRWVLMMTVPGLALFYGGMVRKKNV
LATLMQSFAICCIVTIVWMVAGYSLAFGTGSPYIGDLSRFMLNGIGAQISKGSDVGFTLGLGSANATVMTIPESVFMMFQ
MTFAIITPALITGAFAERMKFSALCVFTILWSLLVYAPVAHWVWSPLGWVAGFGAIDFAGGTVVHINAGVAGLVTALVVG
KRQGYGQDDMSPFNLTYAVIGASLLWVGWFGFNAGSAVGANGRAGMAMATTQIASAAAGVAWMLAEWARTGKPTVLGIIS
GAVGGLVAITPAAGFVLPGGALIIGLLAGAVCYWTATTMKHMLGYDDSLDAFGVHGIGGILGALLTGVLAYGPLSATDAN
PAGVVGSFAQLVTQAKAVGVTIVWCAVVTFVLLKIVDLAIGLRVRSEDEIEGLDMTQHGERIN

Sequences:

>Translated_463_residues
MSLPRTIRTFSPRGTTIALATLAGAALPAMAAPAMAADPAPPAINTGDTPWMLVSTRWVLMMTVPGLALFYGGMVRKKNV
LATLMQSFAICCIVTIVWMVAGYSLAFGTGSPYIGDLSRFMLNGIGAQISKGSDVGFTLGLGSANATVMTIPESVFMMFQ
MTFAIITPALITGAFAERMKFSALCVFTILWSLLVYAPVAHWVWSPLGWVAGFGAIDFAGGTVVHINAGVAGLVTALVVG
KRQGYGQDDMSPFNLTYAVIGASLLWVGWFGFNAGSAVGANGRAGMAMATTQIASAAAGVAWMLAEWARTGKPTVLGIIS
GAVGGLVAITPAAGFVLPGGALIIGLLAGAVCYWTATTMKHMLGYDDSLDAFGVHGIGGILGALLTGVLAYGPLSATDAN
PAGVVGSFAQLVTQAKAVGVTIVWCAVVTFVLLKIVDLAIGLRVRSEDEIEGLDMTQHGERIN
>Mature_462_residues
SLPRTIRTFSPRGTTIALATLAGAALPAMAAPAMAADPAPPAINTGDTPWMLVSTRWVLMMTVPGLALFYGGMVRKKNVL
ATLMQSFAICCIVTIVWMVAGYSLAFGTGSPYIGDLSRFMLNGIGAQISKGSDVGFTLGLGSANATVMTIPESVFMMFQM
TFAIITPALITGAFAERMKFSALCVFTILWSLLVYAPVAHWVWSPLGWVAGFGAIDFAGGTVVHINAGVAGLVTALVVGK
RQGYGQDDMSPFNLTYAVIGASLLWVGWFGFNAGSAVGANGRAGMAMATTQIASAAAGVAWMLAEWARTGKPTVLGIISG
AVGGLVAITPAAGFVLPGGALIIGLLAGAVCYWTATTMKHMLGYDDSLDAFGVHGIGGILGALLTGVLAYGPLSATDANP
AGVVGSFAQLVTQAKAVGVTIVWCAVVTFVLLKIVDLAIGLRVRSEDEIEGLDMTQHGERIN

Specific function: Functions as an ammonium and methylammonium transporter in the absence of glutamine. Required for ammonium utilization at low concentrations or at low pH values, when ammonium is the single nitrogen source. Required for binding of NrgB to the membrane [H]

COG id: COG0004

COG function: function code P; Ammonia permease

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ammonium transporter (TC 2.A.49) family [H]

Homologues:

Organism=Escherichia coli, GI1786656, Length=443, Percent_Identity=44.920993227991, Blast_Score=357, Evalue=1e-99,
Organism=Caenorhabditis elegans, GI17550182, Length=445, Percent_Identity=26.2921348314607, Blast_Score=127, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI17550180, Length=440, Percent_Identity=25.9090909090909, Blast_Score=120, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI133931100, Length=448, Percent_Identity=26.3392857142857, Blast_Score=115, Evalue=4e-26,
Organism=Caenorhabditis elegans, GI32564042, Length=436, Percent_Identity=25.2293577981651, Blast_Score=114, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6324187, Length=426, Percent_Identity=35.9154929577465, Blast_Score=236, Evalue=8e-63,
Organism=Saccharomyces cerevisiae, GI6325396, Length=413, Percent_Identity=34.1404358353511, Blast_Score=223, Evalue=3e-59,
Organism=Saccharomyces cerevisiae, GI6321559, Length=433, Percent_Identity=30.4849884526559, Blast_Score=204, Evalue=3e-53,
Organism=Drosophila melanogaster, GI161078314, Length=437, Percent_Identity=28.604118993135, Blast_Score=140, Evalue=2e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001905
- InterPro:   IPR018047 [H]

Pfam domain/function: PF00909 Ammonium_transp [H]

EC number: NA

Molecular weight: Translated: 47826; Mature: 47695

Theoretical pI: Translated: 7.88; Mature: 7.88

Prosite motif: PS01219 AMMONIUM_TRANSP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLPRTIRTFSPRGTTIALATLAGAALPAMAAPAMAADPAPPAINTGDTPWMLVSTRWVL
CCCCCCHHCCCCCCCEEEHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEECCEEE
MMTVPGLALFYGGMVRKKNVLATLMQSFAICCIVTIVWMVAGYSLAFGTGSPYIGDLSRF
EEHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCHHHHHHH
MLNGIGAQISKGSDVGFTLGLGSANATVMTIPESVFMMFQMTFAIITPALITGAFAERMK
HHHHCCCCCCCCCCCEEEEEECCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FSALCVFTILWSLLVYAPVAHWVWSPLGWVAGFGAIDFAGGTVVHINAGVAGLVTALVVG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCEEEEECCCHHHHHHHHHHH
KRQGYGQDDMSPFNLTYAVIGASLLWVGWFGFNAGSAVGANGRAGMAMATTQIASAAAGV
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
AWMLAEWARTGKPTVLGIISGAVGGLVAITPAAGFVLPGGALIIGLLAGAVCYWTATTMK
HHHHHHHHHCCCCEEEEEHHHHHCCEEEECCCCCEECCCHHHHHHHHHHHHHHHHHHHHH
HMLGYDDSLDAFGVHGIGGILGALLTGVLAYGPLSATDANPAGVVGSFAQLVTQAKAVGV
HHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
TIVWCAVVTFVLLKIVDLAIGLRVRSEDEIEGLDMTQHGERIN
HHHHHHHHHHHHHHHHHHHHCCEECCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SLPRTIRTFSPRGTTIALATLAGAALPAMAAPAMAADPAPPAINTGDTPWMLVSTRWVL
CCCCCHHCCCCCCCEEEHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEECCEEE
MMTVPGLALFYGGMVRKKNVLATLMQSFAICCIVTIVWMVAGYSLAFGTGSPYIGDLSRF
EEHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCHHHHHHH
MLNGIGAQISKGSDVGFTLGLGSANATVMTIPESVFMMFQMTFAIITPALITGAFAERMK
HHHHCCCCCCCCCCCEEEEEECCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FSALCVFTILWSLLVYAPVAHWVWSPLGWVAGFGAIDFAGGTVVHINAGVAGLVTALVVG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCEEEEECCCHHHHHHHHHHH
KRQGYGQDDMSPFNLTYAVIGASLLWVGWFGFNAGSAVGANGRAGMAMATTQIASAAAGV
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
AWMLAEWARTGKPTVLGIISGAVGGLVAITPAAGFVLPGGALIIGLLAGAVCYWTATTMK
HHHHHHHHHCCCCEEEEEHHHHHCCEEEECCCCCEECCCHHHHHHHHHHHHHHHHHHHHH
HMLGYDDSLDAFGVHGIGGILGALLTGVLAYGPLSATDANPAGVVGSFAQLVTQAKAVGV
HHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
TIVWCAVVTFVLLKIVDLAIGLRVRSEDEIEGLDMTQHGERIN
HHHHHHHHHHHHHHHHHHHHCCEECCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NH3 [Periplasm] [C]

Specific reaction: NH3 [Periplasm] = NH3 [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8282685; 9353933; 9384377 [H]