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The map label for this gene is amiA [H]

Identifier: 162148124

GI number: 162148124

Start: 2409390

End: 2410289

Strand: Reverse

Name: amiA [H]

Synonym: GDI_2341

Alternate gene names: 162148124

Gene position: 2410289-2409390 (Counterclockwise)

Preceding gene: 162148128

Following gene: 162148123

Centisome position: 61.11

GC content: 70.0

Gene sequence:

>900_bases
ATGCACAAGGATAGTTCCGATAACGTGACTGCGCGCGGACGCCGGCAGGTCCTGCGGGCCGGCCTGTCCGCGACCGGCCT
GGCGGCGATCCTGGCCGTGCGCCCACGGGTCGCGTTGGCGGCGCAGCGCCGCGTGGCGCACCATGCGCGGCATGCGACGG
GATTGGCCGCGCCCGCCATCGTCGGGCGGGCGGTGGCGCACCTGCCGCTGGTCATGCTCGACCCCGGTCATGGCGGAAAG
GACCCAGGCGAAATATGGGTCTCGGGCACCTACGAAAAACACATCGCCGAGGCCGCGGCGGCCGAACTGCGTCGGCAGTT
GCTGGCCAGCGGCGCCTATCGCGTGGCGATGACGCGGTCGGACGACCGTTTCATTCCGCTGGAAGGTCGGGTGGATATCG
CCCATGCCCACCGGGCGGCCCTGTTCATTTCCATGCATGCCGACGCGCTGACCGATCGCGGGGTGCGCGGGGCCAGCGTC
TACACCCTGTCGGGCCAGGCGTCGGACGCGCAGACCGCGGCGCTGGCGCGCACCGAAAACAGCGCCGACCGCTTCGGCGG
CCCGCAGGTGCATGCCAGTTCGCCCGAGGTGCAGGCCATCCTGGCCAGCCTGGTGACCGAGGAGACGCGGCGCGGCGCCG
CCCACATGGCCAGCAGCGTGGTCGCGTCCTTCCAGTCGCGGATCGGGCTGCTGCCCCATCCGTCCCGCCATGCGGCCTTC
GTCGTGCTGAAATCGGCCGACATCCCCTCGGTGCTGGTCGAAATGGGGTTCATGTCCAACCGGATGGACGAAGCCGCATT
GCGCCAGGCCGGACACAGGATGATGGTCGCCGGGGCCATGAAGCAGGCCATCGACCGTTATTTCGCCTCGGCCAACGGCA
TCACGCACCTGACCGGCTGA

Upstream 100 bases:

>100_bases
GCGTCCGTACCAATGACCCCCCGCAAGTCGTTGAAATGCTGACTTGGGCCACGGACCCTGCTAGGGTGGCGGGCAGGATC
CGCAACATGGACCGGTATTC

Downstream 100 bases:

>100_bases
AATATGGACCGGCCGCCTTGCGTCATCATCCTCAACGGTGTCGGCAGCGTCGGCAAGAGTTCCGTCGCGCGGGCGCTTCA
GGGGCTGCTGGACCGCCCGT

Product: N-acetylmuramoyl-L-alanine amidase amiA precurso

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 299; Mature: 299

Protein sequence:

>299_residues
MHKDSSDNVTARGRRQVLRAGLSATGLAAILAVRPRVALAAQRRVAHHARHATGLAAPAIVGRAVAHLPLVMLDPGHGGK
DPGEIWVSGTYEKHIAEAAAAELRRQLLASGAYRVAMTRSDDRFIPLEGRVDIAHAHRAALFISMHADALTDRGVRGASV
YTLSGQASDAQTAALARTENSADRFGGPQVHASSPEVQAILASLVTEETRRGAAHMASSVVASFQSRIGLLPHPSRHAAF
VVLKSADIPSVLVEMGFMSNRMDEAALRQAGHRMMVAGAMKQAIDRYFASANGITHLTG

Sequences:

>Translated_299_residues
MHKDSSDNVTARGRRQVLRAGLSATGLAAILAVRPRVALAAQRRVAHHARHATGLAAPAIVGRAVAHLPLVMLDPGHGGK
DPGEIWVSGTYEKHIAEAAAAELRRQLLASGAYRVAMTRSDDRFIPLEGRVDIAHAHRAALFISMHADALTDRGVRGASV
YTLSGQASDAQTAALARTENSADRFGGPQVHASSPEVQAILASLVTEETRRGAAHMASSVVASFQSRIGLLPHPSRHAAF
VVLKSADIPSVLVEMGFMSNRMDEAALRQAGHRMMVAGAMKQAIDRYFASANGITHLTG
>Mature_299_residues
MHKDSSDNVTARGRRQVLRAGLSATGLAAILAVRPRVALAAQRRVAHHARHATGLAAPAIVGRAVAHLPLVMLDPGHGGK
DPGEIWVSGTYEKHIAEAAAAELRRQLLASGAYRVAMTRSDDRFIPLEGRVDIAHAHRAALFISMHADALTDRGVRGASV
YTLSGQASDAQTAALARTENSADRFGGPQVHASSPEVQAILASLVTEETRRGAAHMASSVVASFQSRIGLLPHPSRHAAF
VVLKSADIPSVLVEMGFMSNRMDEAALRQAGHRMMVAGAMKQAIDRYFASANGITHLTG

Specific function: Cell-wall hydrolase involved in septum cleavage during cell division. Can also act as powerful autolysin in the presence of murein synthesis inhibitors [H]

COG id: COG0860

COG function: function code M; N-acetylmuramoyl-L-alanine amidase

Gene ontology:

Cell location: Periplasm. Note=Distributed throughout the periplasm in all cells [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 3 family [H]

Homologues:

Organism=Escherichia coli, GI1788776, Length=280, Percent_Identity=36.7857142857143, Blast_Score=149, Evalue=2e-37,
Organism=Escherichia coli, GI87082163, Length=230, Percent_Identity=37.3913043478261, Blast_Score=131, Evalue=6e-32,
Organism=Escherichia coli, GI1790611, Length=270, Percent_Identity=34.0740740740741, Blast_Score=129, Evalue=2e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002508
- InterPro:   IPR006311 [H]

Pfam domain/function: PF01520 Amidase_3 [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 31667; Mature: 31667

Theoretical pI: Translated: 11.09; Mature: 11.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHKDSSDNVTARGRRQVLRAGLSATGLAAILAVRPRVALAAQRRVAHHARHATGLAAPAI
CCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCHHHHHH
VGRAVAHLPLVMLDPGHGGKDPGEIWVSGTYEKHIAEAAAAELRRQLLASGAYRVAMTRS
HHHHHHHCCEEEECCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHCCCEEEEEECC
DDRFIPLEGRVDIAHAHRAALFISMHADALTDRGVRGASVYTLSGQASDAQTAALARTEN
CCCEECCCCCEEHHHHHHEEEEEEECHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHCCC
SADRFGGPQVHASSPEVQAILASLVTEETRRGAAHMASSVVASFQSRIGLLPHPSRHAAF
CHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEE
VVLKSADIPSVLVEMGFMSNRMDEAALRQAGHRMMVAGAMKQAIDRYFASANGITHLTG
EEEECCCCHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCEECCC
>Mature Secondary Structure
MHKDSSDNVTARGRRQVLRAGLSATGLAAILAVRPRVALAAQRRVAHHARHATGLAAPAI
CCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCHHHHHH
VGRAVAHLPLVMLDPGHGGKDPGEIWVSGTYEKHIAEAAAAELRRQLLASGAYRVAMTRS
HHHHHHHCCEEEECCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHCCCEEEEEECC
DDRFIPLEGRVDIAHAHRAALFISMHADALTDRGVRGASVYTLSGQASDAQTAALARTEN
CCCEECCCCCEEHHHHHHEEEEEEECHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHCCC
SADRFGGPQVHASSPEVQAILASLVTEETRRGAAHMASSVVASFQSRIGLLPHPSRHAAF
CHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEE
VVLKSADIPSVLVEMGFMSNRMDEAALRQAGHRMMVAGAMKQAIDRYFASANGITHLTG
EEEECCCCHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8300522; 9205837; 9278503; 1903834 [H]