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The map label for this gene is mrdA [H]

Identifier: 162148117

GI number: 162148117

Start: 2401480

End: 2403384

Strand: Reverse

Name: mrdA [H]

Synonym: GDI_2334

Alternate gene names: 162148117

Gene position: 2403384-2401480 (Counterclockwise)

Preceding gene: 162148118

Following gene: 162148116

Centisome position: 60.94

GC content: 69.03

Gene sequence:

>1905_bases
ATGATCCGGCGCAGGAAAACCGCCACCCGCCTGATGTCGCGGCCCGATACCGGGAATCCGTCGCGCGGGGTCTTCACCCG
GCGCGCGCTGGTGGTGCTGGGGGCGCAGATGGCGGCGCTGGGCGCGCTGGGGCACCGGCTGTACGGACTCCAGGTGCTCG
ACGGCGACCATTACGCCAAGCTGGCGGAAAACAACCGCGTCAATCGCAGCCTGATCGCCCCGCCGCGCGGCCGGGTGGTC
GACCGGTTCGGCATTGCCGTGGCGTCGAACAAGGTCAACTGGCGCGCGCTGCTGCTGCCCGAGGACACCAGCGACATCTC
CGGCACGCTGGACCGGTTTTCCGCCCTGGTGCCCATCGAGGCCCGCGATCGCGCGCGTATCGAGCGCGAGATGCGGCACA
AGCGGCGCTTCATCCCGATCATGCTGCACGACTTCCTGTCGTGGGACGACATGGCGCGGATCGAACTGAATGCGCCCTCG
CTGCCCGGCGTGCTGATCGATGTCGGGACCACCCGCAACTATCCGTTCGGGCCGCTGATGGCCCATGTCGTGGGCTATGT
CGCGCCGCCGGGCGAAAAGGACGTGACGGCCGACGCGACGATGGCCCTGCCGGGAATGCGCATCGGCCGGTCGGGAATCG
AGCAGACGCAGGATACCTTGCTGCGCGGCCAGGTGGGCGCGGTGGAGATCGAGGTCAACGCCGTCGGCCGCGTCATGGCC
GAACTGTCGCGCGATGATGGGCAGGCCGGCGACGAAATCACCCTGACCATCGATTCGGGCCTGCAGCAGGCGGTGCTGGG
GCGCATCGCCGACCAGTCGGCCAGCGCCGTGGTGATGGATTGCCGCAATGGCGAGGTGCTGGCCATGGTCAGCAACCCGT
CCTTCGACCCCTCGCTGTTCGACAGCGGCGTCAGCCAGGCGCAATGGGTCGAATGGACCAACAACCAGGGCACGCCGCTG
ATCAACAAGGCGGTCGCGGGCGTCTATCCCCCCGGCTCGACCTTCAAGCCCGCCGTGGCCATGGCGGCCCTGCAGGCCGG
CACGCTGTCGCCGACCGACCGGATCTTCTGTCCCGGCTACCTGGATGTGGGCGGCACGCGCTTCCATTGCTGGTCGCGTT
ACGGCCACGGATCGCTGACGCTGAAGGACGGGCTGAAATTCTCCTGCGACGTGTTCTTCTACGAAGCCGCGCGGCGGACC
GGCATGGACGCCATCGCCGCGGCGTCGCACGCGTTCGGCCTGGGCACGCACCTGGATATCGAACTGCCGCACACCCGCCA
GGGATTGATCCCGACCCCGGACTGGCGGCGGGCGCACGGCCAGCACTGGAATGGCGGCGATACGATCGTCAGCGGCATCG
GGCAGGGATTCGTGCAGGTCACGCCGCTCCAACTCGCCACCTATGCATCGCGGATCGCCAGCGGGCGGGCGGTGCAGCCG
CATCTGGTCCGCGCGGTCGCGGGCAAGCTGGCGGAGGGCACGGACCCCACGCACTGGCCGGCGCTGGGCATGCCCGATCC
GTTCCTGGGGCAGTTGCGCGCCGGCATGTTCGCCGTGATCAACGAGGAACACGGCACCGCGCCCAAGGCGCGCCTGGATA
TTCCAGGCGTCCAGATGGCCGGCAAGACCGGGTCCGCGCAGGTGCGTCGCGTGTCGCGCGCCCTGCGCGAAAGCGGGCAT
TTCGATTCCTCCGCCCTGCCGTGGGAATATCGCCCGCATGCCCTGTTCATCTGCTATGCGCCCTATGACGCGCCGCGCTA
TGCGGTTTCGGTGGTGGTGGAACACGGCAATGCGGGCGCCGCGGTTGCGGCGCCGCTGGCCCGCGATATCATGACCGATG
CGCTGACCCGCGACCCGGCGAACCGCAAGGAACCGCCGGGCCAGGTGGTGGCCGACATCGAATAG

Upstream 100 bases:

>100_bases
TTCGCCGCCATGGCGCGCACGATCGCCAATCCCGGCCGCGCCTGACGTCCCCCTTGCGCGCGACTCATGACGTATTCTCT
GTCCTGACGCGGATGGGTGG

Downstream 100 bases:

>100_bases
TCGCCGGTGGGCTGCGTTACCCCTGCCGGAGAGGGACACATGAATTTCCAGAAGCGCCTCCTGCGCGCCGAACCCAATTT
CCGCATCCTGGCCAAGCTGT

Product: cell elongation specific D,D-transpeptidase

Products: NA

Alternate protein names: PBP-2 [H]

Number of amino acids: Translated: 634; Mature: 634

Protein sequence:

>634_residues
MIRRRKTATRLMSRPDTGNPSRGVFTRRALVVLGAQMAALGALGHRLYGLQVLDGDHYAKLAENNRVNRSLIAPPRGRVV
DRFGIAVASNKVNWRALLLPEDTSDISGTLDRFSALVPIEARDRARIEREMRHKRRFIPIMLHDFLSWDDMARIELNAPS
LPGVLIDVGTTRNYPFGPLMAHVVGYVAPPGEKDVTADATMALPGMRIGRSGIEQTQDTLLRGQVGAVEIEVNAVGRVMA
ELSRDDGQAGDEITLTIDSGLQQAVLGRIADQSASAVVMDCRNGEVLAMVSNPSFDPSLFDSGVSQAQWVEWTNNQGTPL
INKAVAGVYPPGSTFKPAVAMAALQAGTLSPTDRIFCPGYLDVGGTRFHCWSRYGHGSLTLKDGLKFSCDVFFYEAARRT
GMDAIAAASHAFGLGTHLDIELPHTRQGLIPTPDWRRAHGQHWNGGDTIVSGIGQGFVQVTPLQLATYASRIASGRAVQP
HLVRAVAGKLAEGTDPTHWPALGMPDPFLGQLRAGMFAVINEEHGTAPKARLDIPGVQMAGKTGSAQVRRVSRALRESGH
FDSSALPWEYRPHALFICYAPYDAPRYAVSVVVEHGNAGAAVAAPLARDIMTDALTRDPANRKEPPGQVVADIE

Sequences:

>Translated_634_residues
MIRRRKTATRLMSRPDTGNPSRGVFTRRALVVLGAQMAALGALGHRLYGLQVLDGDHYAKLAENNRVNRSLIAPPRGRVV
DRFGIAVASNKVNWRALLLPEDTSDISGTLDRFSALVPIEARDRARIEREMRHKRRFIPIMLHDFLSWDDMARIELNAPS
LPGVLIDVGTTRNYPFGPLMAHVVGYVAPPGEKDVTADATMALPGMRIGRSGIEQTQDTLLRGQVGAVEIEVNAVGRVMA
ELSRDDGQAGDEITLTIDSGLQQAVLGRIADQSASAVVMDCRNGEVLAMVSNPSFDPSLFDSGVSQAQWVEWTNNQGTPL
INKAVAGVYPPGSTFKPAVAMAALQAGTLSPTDRIFCPGYLDVGGTRFHCWSRYGHGSLTLKDGLKFSCDVFFYEAARRT
GMDAIAAASHAFGLGTHLDIELPHTRQGLIPTPDWRRAHGQHWNGGDTIVSGIGQGFVQVTPLQLATYASRIASGRAVQP
HLVRAVAGKLAEGTDPTHWPALGMPDPFLGQLRAGMFAVINEEHGTAPKARLDIPGVQMAGKTGSAQVRRVSRALRESGH
FDSSALPWEYRPHALFICYAPYDAPRYAVSVVVEHGNAGAAVAAPLARDIMTDALTRDPANRKEPPGQVVADIE
>Mature_634_residues
MIRRRKTATRLMSRPDTGNPSRGVFTRRALVVLGAQMAALGALGHRLYGLQVLDGDHYAKLAENNRVNRSLIAPPRGRVV
DRFGIAVASNKVNWRALLLPEDTSDISGTLDRFSALVPIEARDRARIEREMRHKRRFIPIMLHDFLSWDDMARIELNAPS
LPGVLIDVGTTRNYPFGPLMAHVVGYVAPPGEKDVTADATMALPGMRIGRSGIEQTQDTLLRGQVGAVEIEVNAVGRVMA
ELSRDDGQAGDEITLTIDSGLQQAVLGRIADQSASAVVMDCRNGEVLAMVSNPSFDPSLFDSGVSQAQWVEWTNNQGTPL
INKAVAGVYPPGSTFKPAVAMAALQAGTLSPTDRIFCPGYLDVGGTRFHCWSRYGHGSLTLKDGLKFSCDVFFYEAARRT
GMDAIAAASHAFGLGTHLDIELPHTRQGLIPTPDWRRAHGQHWNGGDTIVSGIGQGFVQVTPLQLATYASRIASGRAVQP
HLVRAVAGKLAEGTDPTHWPALGMPDPFLGQLRAGMFAVINEEHGTAPKARLDIPGVQMAGKTGSAQVRRVSRALRESGH
FDSSALPWEYRPHALFICYAPYDAPRYAVSVVVEHGNAGAAVAAPLARDIMTDALTRDPANRKEPPGQVVADIE

Specific function: Cell wall formation; PBP-2 is responsible for the determination of the rod shape of the cell. Its synthesize cross- linked peptidoglycan from the lipid intermediates [H]

COG id: COG0768

COG function: function code M; Cell division protein FtsI/penicillin-binding protein 2

Gene ontology:

Cell location: Cell inner membrane [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI1786854, Length=606, Percent_Identity=35.3135313531353, Blast_Score=346, Evalue=3e-96,
Organism=Escherichia coli, GI1786272, Length=619, Percent_Identity=27.4636510500808, Blast_Score=132, Evalue=5e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR005311
- InterPro:   IPR001460
- InterPro:   IPR017790 [H]

Pfam domain/function: PF03717 PBP_dimer; PF00905 Transpeptidase [H]

EC number: NA

Molecular weight: Translated: 68520; Mature: 68520

Theoretical pI: Translated: 8.47; Mature: 8.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRRRKTATRLMSRPDTGNPSRGVFTRRALVVLGAQMAALGALGHRLYGLQVLDGDHYAK
CCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHEEEEEEECCCCHHH
LAENNRVNRSLIAPPRGRVVDRFGIAVASNKVNWRALLLPEDTSDISGTLDRFSALVPIE
HHHCCCCCCEEECCCCCCEEHHHCEEEECCCCCEEEEEECCCCCHHHHHHHHHHEECCCC
ARDRARIEREMRHKRRFIPIMLHDFLSWDDMARIELNAPSLPGVLIDVGTTRNYPFGPLM
CHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCEEEEECCCCCCEEEEECCCCCCCCCHHHH
AHVVGYVAPPGEKDVTADATMALPGMRIGRSGIEQTQDTLLRGQVGAVEIEVNAVGRVMA
HHHHHHCCCCCCCCCCCCCCEECCCCHHCHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHH
ELSRDDGQAGDEITLTIDSGLQQAVLGRIADQSASAVVMDCRNGEVLAMVSNPSFDPSLF
HHHCCCCCCCCEEEEEECCCHHHHHHHHHHCCCCCEEEEECCCCCEEEEECCCCCCHHHH
DSGVSQAQWVEWTNNQGTPLINKAVAGVYPPGSTFKPAVAMAALQAGTLSPTDRIFCPGY
HCCCCCCEEEEECCCCCCCHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCCCCEECCCE
LDVGGTRFHCWSRYGHGSLTLKDGLKFSCDVFFYEAARRTGMDAIAAASHAFGLGTHLDI
ECCCCCEEEEEECCCCCEEEECCCCEEEEEEEEEEHHHHCCCHHHHHHHHHCCCCCEEEE
ELPHTRQGLIPTPDWRRAHGQHWNGGDTIVSGIGQGFVQVTPLQLATYASRIASGRAVQP
ECCCCCCCCCCCCCHHHHCCCCCCCHHHHHHHHCCCCEEECHHHHHHHHHHHHCCCCCCH
HLVRAVAGKLAEGTDPTHWPALGMPDPFLGQLRAGMFAVINEEHGTAPKARLDIPGVQMA
HHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHCCEEEEEECCCCCCCCCEECCCCCEEC
GKTGSAQVRRVSRALRESGHFDSSALPWEYRPHALFICYAPYDAPRYAVSVVVEHGNAGA
CCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEECCCCCCCEEEEEEEECCCCCC
AVAAPLARDIMTDALTRDPANRKEPPGQVVADIE
HHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECC
>Mature Secondary Structure
MIRRRKTATRLMSRPDTGNPSRGVFTRRALVVLGAQMAALGALGHRLYGLQVLDGDHYAK
CCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHEEEEEEECCCCHHH
LAENNRVNRSLIAPPRGRVVDRFGIAVASNKVNWRALLLPEDTSDISGTLDRFSALVPIE
HHHCCCCCCEEECCCCCCEEHHHCEEEECCCCCEEEEEECCCCCHHHHHHHHHHEECCCC
ARDRARIEREMRHKRRFIPIMLHDFLSWDDMARIELNAPSLPGVLIDVGTTRNYPFGPLM
CHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCEEEEECCCCCCEEEEECCCCCCCCCHHHH
AHVVGYVAPPGEKDVTADATMALPGMRIGRSGIEQTQDTLLRGQVGAVEIEVNAVGRVMA
HHHHHHCCCCCCCCCCCCCCEECCCCHHCHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHH
ELSRDDGQAGDEITLTIDSGLQQAVLGRIADQSASAVVMDCRNGEVLAMVSNPSFDPSLF
HHHCCCCCCCCEEEEEECCCHHHHHHHHHHCCCCCEEEEECCCCCEEEEECCCCCCHHHH
DSGVSQAQWVEWTNNQGTPLINKAVAGVYPPGSTFKPAVAMAALQAGTLSPTDRIFCPGY
HCCCCCCEEEEECCCCCCCHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCCCCEECCCE
LDVGGTRFHCWSRYGHGSLTLKDGLKFSCDVFFYEAARRTGMDAIAAASHAFGLGTHLDI
ECCCCCEEEEEECCCCCEEEECCCCEEEEEEEEEEHHHHCCCHHHHHHHHHCCCCCEEEE
ELPHTRQGLIPTPDWRRAHGQHWNGGDTIVSGIGQGFVQVTPLQLATYASRIASGRAVQP
ECCCCCCCCCCCCCHHHHCCCCCCCHHHHHHHHCCCCEEECHHHHHHHHHHHHCCCCCCH
HLVRAVAGKLAEGTDPTHWPALGMPDPFLGQLRAGMFAVINEEHGTAPKARLDIPGVQMA
HHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHCCEEEEEECCCCCCCCCEECCCCCEEC
GKTGSAQVRRVSRALRESGHFDSSALPWEYRPHALFICYAPYDAPRYAVSVVVEHGNAGA
CCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEECCCCCCCEEEEEEEECCCCCC
AVAAPLARDIMTDALTRDPANRKEPPGQVVADIE
HHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]