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The map label for this gene is pinE [C]

Identifier: 162148031

GI number: 162148031

Start: 2304459

End: 2305064

Strand: Direct

Name: pinE [C]

Synonym: GDI_2247

Alternate gene names: 162148031

Gene position: 2304459-2305064 (Clockwise)

Preceding gene: 162148030

Following gene: 162148037

Centisome position: 58.43

GC content: 61.06

Gene sequence:

>606_bases
TTGAGATACCATCTCAATGAGGTCTTAGATTCTATGGAGATGGGCATGAGGATCGGATATGCTCGGACCAGCACGACGGA
GCAGGCGGCGGGGTTGGCGGCGCAAGTCCGTGATCTCGAAGCGCAGGGATGCAATCGGGTCTTCGCGGAACAAGTGTCAG
GCACCATCACTGTCAGGCCGGAACTGGAGGCGGCCATCGACTATCTGCGCCAAGGCGATGCGTTGGTGGTGACGAAGCCC
GACCGCTTGGCGCGATCGACGACGGACTTACTGTCGTTCGTGCATCGGGTGCAGGCCAAGGGCGCTGATCTGGTTATCCT
GTCTATGGGGGGGCAGCCTTTGGATACCAGCAGTCCGACCAGCAAGCTGACGCTGACCTTGCTGGCAGCCGTAGCCGAAT
TCGAGCGCGACATTATGAAGGAGCGGCAGGCGGAGGGGATCGCAAAAGCGAAGGCTGAAGGAAAATATCGCGGACGCAAG
CCGACCGCGCGCGCGAAGGCCGTCCATGTCAGGGCGATGTCAGCCGAAGGTGTCAGTCCTACCGAAATCGCGAAGCGGCT
GGGGATCGGTCGGGCTTCGGTCTACCGGTGCCTAGAGGTCGCGTGA

Upstream 100 bases:

>100_bases
TGTCCCATTCTGAACGTCTCATTAGGGCAGGGTCGTATGAGATGATTACGATGCAGGCGACAATCATCATTTTCTGTCTC
ACACGGATTGAAATCTCTTT

Downstream 100 bases:

>100_bases
TGAACGGCTTCCGACGGCCTGCCATAAGCGCATGTTAGACCGGCAGCTATGCGGGAGGGGAGGAAGCGGAATGTTTGGTT
CGGGTCTGCGAGGGTAGAAA

Product: resolvase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 201; Mature: 201

Protein sequence:

>201_residues
MRYHLNEVLDSMEMGMRIGYARTSTTEQAAGLAAQVRDLEAQGCNRVFAEQVSGTITVRPELEAAIDYLRQGDALVVTKP
DRLARSTTDLLSFVHRVQAKGADLVILSMGGQPLDTSSPTSKLTLTLLAAVAEFERDIMKERQAEGIAKAKAEGKYRGRK
PTARAKAVHVRAMSAEGVSPTEIAKRLGIGRASVYRCLEVA

Sequences:

>Translated_201_residues
MRYHLNEVLDSMEMGMRIGYARTSTTEQAAGLAAQVRDLEAQGCNRVFAEQVSGTITVRPELEAAIDYLRQGDALVVTKP
DRLARSTTDLLSFVHRVQAKGADLVILSMGGQPLDTSSPTSKLTLTLLAAVAEFERDIMKERQAEGIAKAKAEGKYRGRK
PTARAKAVHVRAMSAEGVSPTEIAKRLGIGRASVYRCLEVA
>Mature_201_residues
MRYHLNEVLDSMEMGMRIGYARTSTTEQAAGLAAQVRDLEAQGCNRVFAEQVSGTITVRPELEAAIDYLRQGDALVVTKP
DRLARSTTDLLSFVHRVQAKGADLVILSMGGQPLDTSSPTSKLTLTLLAAVAEFERDIMKERQAEGIAKAKAEGKYRGRK
PTARAKAVHVRAMSAEGVSPTEIAKRLGIGRASVYRCLEVA

Specific function: This Protein Catalyzes The Inversion Of An 1800-Bp E.Coli DNA Fragment, The P Region, Which Can Exist In Either Orientation. The Function Of The Inversion Is Not Yet Clear. [C]

COG id: COG1961

COG function: function code L; Site-specific recombinases, DNA invertase Pin homologs

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the site-specific recombinase resolvase family [H]

Homologues:

Organism=Escherichia coli, GI1787404, Length=146, Percent_Identity=36.986301369863, Blast_Score=97, Evalue=8e-22,
Organism=Escherichia coli, GI1787827, Length=192, Percent_Identity=33.8541666666667, Blast_Score=93, Evalue=1e-20,
Organism=Escherichia coli, GI1787638, Length=188, Percent_Identity=33.5106382978723, Blast_Score=91, Evalue=5e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR006118
- InterPro:   IPR006119
- InterPro:   IPR006120 [H]

Pfam domain/function: PF02796 HTH_7; PF00239 Resolvase [H]

EC number: NA

Molecular weight: Translated: 21817; Mature: 21817

Theoretical pI: Translated: 9.70; Mature: 9.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRYHLNEVLDSMEMGMRIGYARTSTTEQAAGLAAQVRDLEAQGCNRVFAEQVSGTITVRP
CCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECC
ELEAAIDYLRQGDALVVTKPDRLARSTTDLLSFVHRVQAKGADLVILSMGGQPLDTSSPT
CHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCH
SKLTLTLLAAVAEFERDIMKERQAEGIAKAKAEGKYRGRKPTARAKAVHVRAMSAEGVSP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHEEEEECCCCCCH
TEIAKRLGIGRASVYRCLEVA
HHHHHHHCCCHHHHHHHHHCC
>Mature Secondary Structure
MRYHLNEVLDSMEMGMRIGYARTSTTEQAAGLAAQVRDLEAQGCNRVFAEQVSGTITVRP
CCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECC
ELEAAIDYLRQGDALVVTKPDRLARSTTDLLSFVHRVQAKGADLVILSMGGQPLDTSSPT
CHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCH
SKLTLTLLAAVAEFERDIMKERQAEGIAKAKAEGKYRGRKPTARAKAVHVRAMSAEGVSP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHEEEEECCCCCCH
TEIAKRLGIGRASVYRCLEVA
HHHHHHHCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9163424 [H]