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The map label for this gene is dosC [H]

Identifier: 162148023

GI number: 162148023

Start: 2295802

End: 2297217

Strand: Direct

Name: dosC [H]

Synonym: GDI_2239

Alternate gene names: 162148023

Gene position: 2295802-2297217 (Clockwise)

Preceding gene: 162148012

Following gene: 162148026

Centisome position: 58.21

GC content: 65.18

Gene sequence:

>1416_bases
ATGTCAGTTGCCCAGGTGGCCGTAGACGCCGAATTGCGGCGTGCCTTCACGAACGTGCCGGATGCCACGCGCCTTCTGGT
CCATGACGTCGTCGAACGGCTGACGCCCGCACTGGTCGCGGCGTTCTACGACCATTTCACGTCCCATCCCGAAGGCCAGA
CCTACCTGACAGATCCGGAGACGACGGAGCGCCTGCGCGGCACCCTGATGGTGTGGATGGCCGAACTGTTTTCCGCGGCG
CCGCAGCACCCGCAGCGGATGGCCGATTCGCAGCGGCATATCGGGCGCTTCCACGCACGCTCGTCCATTCCCATCCATCT
GGTGTCCGAGGGCGTTTCGCTGGTCCAGCGAACGATCATCCACGCGCTGGGTCATGAAAACCTGACCCACGAGGAACTGA
CCGACGCGGTACTGTACGTCACCAACGTGCTGACGGCCGCGACCGGGGTGATGACGTTCACCTATTGGCGGCAGCGCGAA
CGCGCGGCGTCGAACGACGTGGCGTACCGGCTGTTCGCGCTGAACCAGGACCTGTCGCGCGAGGGCGAGGGCCAGAAGGC
CGCGCTGCTGGGATGGAGTTATAACGTCCTGCGGTCGCTGTATGTCGCCAAGACGCCCGAGGATTTGCAGACGATGGGGC
TGGCCCGGTCGGAATTCGGCCTGTGGGTGACGTACAAGGTCGGCCTGCTGTTCGAAGGGACGGATATTTCGGCCCTGCTG
ACCGGCCAGATCGCCAAGGTCGATAACGAATTGCTGCCGGCCATATTGGAAGACATGGCGGCGGAACGCGATATTTCGCC
CGCCCTGGCCGCGCTGCATGCCGGCGTCACCGAAATCCTGAGCAGCCTCGAGGTCCTGTTCCACGCCAGCCGGAGCAGCA
ACGATGTCGAGGACCCGCTGACCCGCGTGCTGAACCGGCGTTTCCTGCCGTCGATCATATCCTACGAACTGCGCGTCGCC
CATCAGACGGGGCGGCCGATGAGCGTGCTGTTCCTGGATATCGACCATTTCAAGCACATCAACGACACGTTCGGCCACGC
GGCCGGCGACTCCGTCCTGCAGGCCATCGCCCGCGTGCTGGAGGAAGCCAGCCGGCTGAGCGACGTCATCTTCCGCTATG
GCGGCGAGGAATTCCTGGTCCTCATGGGCGATACCGATACCGAGCAGATGCTCATCGGTGCCGAGCGCCTGCGCCAGAAG
ATCGAGAACACGCCCATCCGACTGCCGGACGGCAACCTGCACCAGATCACGGTGTCGATCGGCGCGGCCCAGTATGCCGG
CCACCCGGATTACGAGGAACTGCTCCGTGCCGCCGACGCCGCGCTCTATCGGGCGAAGCGGAACGGGCGCAACCGGATCG
AGGTCGCCGACGCGCCGCACCATACGCCCGCCCCGAGCCTGGAGGGTGCGTCCTGA

Upstream 100 bases:

>100_bases
TTGCTGCTCGTCCGGACCCGATGCGACCTGTTCATCCCGCCGGCGGCGCCGTCCGGCTTGCCGGATCCCTTCCCCCGTTC
GCCGTGCGAGGCCGAGCATG

Downstream 100 bases:

>100_bases
TCCGGCTGAGGCCGTAACCGGATTCACCAGACCTAGGCGGCGGCCCGGTCGGTCGGGGGGCGATCGGAATCGGCCGCGCG
CCGCCCCTGCGTTACGCGTA

Product: GGDEF family signaling protein

Products: NA

Alternate protein names: DGC [H]

Number of amino acids: Translated: 471; Mature: 470

Protein sequence:

>471_residues
MSVAQVAVDAELRRAFTNVPDATRLLVHDVVERLTPALVAAFYDHFTSHPEGQTYLTDPETTERLRGTLMVWMAELFSAA
PQHPQRMADSQRHIGRFHARSSIPIHLVSEGVSLVQRTIIHALGHENLTHEELTDAVLYVTNVLTAATGVMTFTYWRQRE
RAASNDVAYRLFALNQDLSREGEGQKAALLGWSYNVLRSLYVAKTPEDLQTMGLARSEFGLWVTYKVGLLFEGTDISALL
TGQIAKVDNELLPAILEDMAAERDISPALAALHAGVTEILSSLEVLFHASRSSNDVEDPLTRVLNRRFLPSIISYELRVA
HQTGRPMSVLFLDIDHFKHINDTFGHAAGDSVLQAIARVLEEASRLSDVIFRYGGEEFLVLMGDTDTEQMLIGAERLRQK
IENTPIRLPDGNLHQITVSIGAAQYAGHPDYEELLRAADAALYRAKRNGRNRIEVADAPHHTPAPSLEGAS

Sequences:

>Translated_471_residues
MSVAQVAVDAELRRAFTNVPDATRLLVHDVVERLTPALVAAFYDHFTSHPEGQTYLTDPETTERLRGTLMVWMAELFSAA
PQHPQRMADSQRHIGRFHARSSIPIHLVSEGVSLVQRTIIHALGHENLTHEELTDAVLYVTNVLTAATGVMTFTYWRQRE
RAASNDVAYRLFALNQDLSREGEGQKAALLGWSYNVLRSLYVAKTPEDLQTMGLARSEFGLWVTYKVGLLFEGTDISALL
TGQIAKVDNELLPAILEDMAAERDISPALAALHAGVTEILSSLEVLFHASRSSNDVEDPLTRVLNRRFLPSIISYELRVA
HQTGRPMSVLFLDIDHFKHINDTFGHAAGDSVLQAIARVLEEASRLSDVIFRYGGEEFLVLMGDTDTEQMLIGAERLRQK
IENTPIRLPDGNLHQITVSIGAAQYAGHPDYEELLRAADAALYRAKRNGRNRIEVADAPHHTPAPSLEGAS
>Mature_470_residues
SVAQVAVDAELRRAFTNVPDATRLLVHDVVERLTPALVAAFYDHFTSHPEGQTYLTDPETTERLRGTLMVWMAELFSAAP
QHPQRMADSQRHIGRFHARSSIPIHLVSEGVSLVQRTIIHALGHENLTHEELTDAVLYVTNVLTAATGVMTFTYWRQRER
AASNDVAYRLFALNQDLSREGEGQKAALLGWSYNVLRSLYVAKTPEDLQTMGLARSEFGLWVTYKVGLLFEGTDISALLT
GQIAKVDNELLPAILEDMAAERDISPALAALHAGVTEILSSLEVLFHASRSSNDVEDPLTRVLNRRFLPSIISYELRVAH
QTGRPMSVLFLDIDHFKHINDTFGHAAGDSVLQAIARVLEEASRLSDVIFRYGGEEFLVLMGDTDTEQMLIGAERLRQKI
ENTPIRLPDGNLHQITVSIGAAQYAGHPDYEELLRAADAALYRAKRNGRNRIEVADAPHHTPAPSLEGAS

Specific function: Overexpression leads to an increased level of c-di-GMP, which leads to changes in the cell surface, to abnormal cell division, increased biofilm formation and decreased swimming (the latter 2 in strain W3110). In a strain able to produce cellulose (strain

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI145693134, Length=428, Percent_Identity=31.0747663551402, Blast_Score=234, Evalue=7e-63,
Organism=Escherichia coli, GI1787262, Length=201, Percent_Identity=36.81592039801, Blast_Score=127, Evalue=2e-30,
Organism=Escherichia coli, GI87081881, Length=159, Percent_Identity=41.5094339622642, Blast_Score=118, Evalue=7e-28,
Organism=Escherichia coli, GI1786584, Length=162, Percent_Identity=40.7407407407407, Blast_Score=115, Evalue=5e-27,
Organism=Escherichia coli, GI87082007, Length=162, Percent_Identity=37.037037037037, Blast_Score=100, Evalue=3e-22,
Organism=Escherichia coli, GI1787816, Length=258, Percent_Identity=28.6821705426357, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1787802, Length=131, Percent_Identity=38.1679389312977, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1788956, Length=151, Percent_Identity=37.7483443708609, Blast_Score=89, Evalue=7e-19,
Organism=Escherichia coli, GI1788381, Length=192, Percent_Identity=29.6875, Blast_Score=83, Evalue=4e-17,
Organism=Escherichia coli, GI1788085, Length=172, Percent_Identity=31.3953488372093, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1787056, Length=157, Percent_Identity=30.5732484076433, Blast_Score=75, Evalue=7e-15,
Organism=Escherichia coli, GI1787541, Length=159, Percent_Identity=30.188679245283, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI87081977, Length=166, Percent_Identity=30.7228915662651, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI87081974, Length=161, Percent_Identity=26.0869565217391, Blast_Score=71, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160
- InterPro:   IPR012292
- InterPro:   IPR009050 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 52281; Mature: 52150

Theoretical pI: Translated: 5.76; Mature: 5.76

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVAQVAVDAELRRAFTNVPDATRLLVHDVVERLTPALVAAFYDHFTSHPEGQTYLTDPE
CCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCH
TTERLRGTLMVWMAELFSAAPQHPQRMADSQRHIGRFHARSSIPIHLVSEGVSLVQRTII
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHH
HALGHENLTHEELTDAVLYVTNVLTAATGVMTFTYWRQRERAASNDVAYRLFALNQDLSR
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHC
EGEGQKAALLGWSYNVLRSLYVAKTPEDLQTMGLARSEFGLWVTYKVGLLFEGTDISALL
CCCCCEEEEECCCHHHHHHHHHCCCCHHHHHHCCHHHHCCEEEEEEEEEEEECCCCHHHH
TGQIAKVDNELLPAILEDMAAERDISPALAALHAGVTEILSSLEVLFHASRSSNDVEDPL
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
TRVLNRRFLPSIISYELRVAHQTGRPMSVLFLDIDHFKHINDTFGHAAGDSVLQAIARVL
HHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEHHHHHHHHHHHCCCCHHHHHHHHHHHH
EEASRLSDVIFRYGGEEFLVLMGDTDTEQMLIGAERLRQKIENTPIRLPDGNLHQITVSI
HHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHCCCCEECCCCCEEEEEEEE
GAAQYAGHPDYEELLRAADAALYRAKRNGRNRIEVADAPHHTPAPSLEGAS
CCHHHCCCCCHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SVAQVAVDAELRRAFTNVPDATRLLVHDVVERLTPALVAAFYDHFTSHPEGQTYLTDPE
CHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCH
TTERLRGTLMVWMAELFSAAPQHPQRMADSQRHIGRFHARSSIPIHLVSEGVSLVQRTII
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHH
HALGHENLTHEELTDAVLYVTNVLTAATGVMTFTYWRQRERAASNDVAYRLFALNQDLSR
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHC
EGEGQKAALLGWSYNVLRSLYVAKTPEDLQTMGLARSEFGLWVTYKVGLLFEGTDISALL
CCCCCEEEEECCCHHHHHHHHHCCCCHHHHHHCCHHHHCCEEEEEEEEEEEECCCCHHHH
TGQIAKVDNELLPAILEDMAAERDISPALAALHAGVTEILSSLEVLFHASRSSNDVEDPL
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
TRVLNRRFLPSIISYELRVAHQTGRPMSVLFLDIDHFKHINDTFGHAAGDSVLQAIARVL
HHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEHHHHHHHHHHHCCCCHHHHHHHHHHHH
EEASRLSDVIFRYGGEEFLVLMGDTDTEQMLIGAERLRQKIENTPIRLPDGNLHQITVSI
HHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHCCCCEECCCCCEEEEEEEE
GAAQYAGHPDYEELLRAADAALYRAKRNGRNRIEVADAPHHTPAPSLEGAS
CCHHHCCCCCHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]